Abstract：
:Phosphatidylinositol catabolism in Saccharomyces cerevisiae cells cultured in media containing inositol results in the release of glycerophosphoinositol (GroPIns) into the medium. As the extracellular concentration of inositol decreases with growth, the released GroPIns is transported back into the cell. Exploiting the ability of the inositol auxotroph, ino1, to use exogenous GroPIns as an inositol source, we have isolated mutants (Git-) defective in the uptake and metabolism of GroPIns. One mutant was found to be affected in the gene encoding the transcription factor, SPT7. Mutants of the positive regulatory gene INO2, but not of its partner, INO4, also have the Git- phenotype. Another mutant was complemented by a single open reading frame (ORF) termed GIT1 (glycerophosphoinositol). This ORF consists of 1556 bp predicted to encode a polypeptide of 518 amino acids and 57.3 kD. The predicted Git1p has similarity to a variety of S. cerevisiae transporters, including a phosphate transporter (Pho84p), and both inositol transporters (Itr1p and Itr2p). Furthermore, Git1p contains a sugar transport motif and 12 potential membrane-spanning domains. Transport assays performed on a git1 mutant together with the above evidence indicate that the GIT1 gene encodes a permease involved in the uptake of GroPIns.
journal_name
Geneticsjournal_title
Geneticsauthors
Patton-Vogt JL,Henry SAsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
1707-15issue
4eissn
0016-6731issn
1943-2631journal_volume
149pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The process of close recombinant formation in bacteriophage T5 crosses has been studied by examining the structure of internal heterozygotes (HETs), the immediate products of recombination events. The T5 system was chosen because it permits the study of internal heterozygotes exclusively, thus avoiding the ambiguities...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：:Life-history traits or "fitness components"-such as age and size at maturity, fecundity and fertility, age-specific rates of survival, and life span-are the major phenotypic determinants of Darwinian fitness. Analyzing the evolution and genetics of these phenotypic targets of selection is central to our understanding ...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.119.300160
更新日期：2020-01-01 00:00:00
abstract：:We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verifi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.076216
更新日期：2007-09-01 00:00:00
abstract：:Telomere position effects on transcription (TPE, or telomeric silencing) are nucleated by association of nonhistone silencing factors with the telomere and propagated in subtelomeric regions through association of silencing factors with the specifically modified histones H3 and H4. However, the function of histone H2A...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:The range of possible gene interactions in a multilocus model of a complex inherited disease is studied by exploring genotype-specific risks subject to the constraint that the allele frequencies and marginal risks are known. We quantify the effect of gene interactions by defining the interaction ratio, CR=KR/KRI, wher...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.119008
更新日期：2010-12-01 00:00:00
abstract：:Mitotic cell divisions increase cell number while faithfully distributing the replicated genome at each division. The Caenorhabditis elegans embryo is a powerful model for eukaryotic cell division. Nearly all of the genes that regulate cell division in C. elegans are conserved across metazoan species, including humans...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.118.301367
更新日期：2019-01-01 00:00:00
abstract：:Vegetative incompatibility in fungi has long been known to reduce the transmission of viruses between individuals, but the barrier to transmission is incomplete. In replicated laboratory assays, we showed conclusively that the transmission of viruses between individuals of the chestnut blight fungus Cryphonectria para...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Sir2 is a highly conserved NAD+-dependent histone deacetylase that functions in heterochromatin formation and promotes replicative life span (RLS) in the budding yeast, Saccharomyces cerevisiae Within the yeast rDNA locus, Sir2 is required for efficient cohesin recruitment and maintaining the stability of the tandem a...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302047
更新日期：2019-05-01 00:00:00
abstract：:Sequences of Bronze2 (Bz2), a maize gene which is required for the synthesis of the purple pigment anthocyanin, have been cloned by combining the techniques of transposon tagging and differential hybridization. First, a mutable bz2 allele (bz2-mu1) was recovered from a Mutator line. The mutation was assumed to result ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:We present a two-part system for conditional FLP-out of FRT-flanked sequences in Caenorhabditis elegans to control gene activity in a spatially and/or temporally regulated manner. Using reporters, we assess the system for efficacy and demonstrate its use as a cell lineage marking tool. In addition, we construct and te...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.090274
更新日期：2008-09-01 00:00:00
abstract：:At pH 6.8, a substantial fraction of recombinant colonies obtained from conjugation with an HfrH donor contained multiple recombinant classes in a single colony (polygenotype colony). In contrast, when the conjugation was performed at pH 7.6, the number of polygenotypic colonies was drastically reduced, and the recomb...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：:The bristle worm Platynereis dumerilii displays many interesting biological characteristics. These include its reproductive timing, which is synchronized to the moon phase, its regenerative capacity that is hormonally controlled, and a slow rate of evolution, which permits analyses of ancestral genes and cell types. A...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.112.148254
更新日期：2014-05-01 00:00:00
abstract：:We estimated DNA sequence variation in a 5.7-kb fragment of the furrowed (fw) gene region within and between four populations of Drosophila ananassae; fw is located in a chromosomal region of very low recombination. We analyzed gene flow between these four populations along a latitudinal transect on the Indian subcont...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:Dna2 is a nuclease and helicase that functions redundantly with other proteins in Okazaki fragment processing, double-strand break resection, and checkpoint kinase activation. Dna2 is an essential enzyme, required for yeast and mammalian cell viability. Here, we report that numerous mutations affecting the DNA damage ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300809
更新日期：2018-05-01 00:00:00
abstract：:The sojourn times until fixation of an overdominant allele were investigated based on the diffusion equation. Furthermore, the rate of accumulation of mutations, or the substitution rate, was predicted from the mean extinction time of a common overdominant allele. The substitution rate calculated theoretically agreed ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitos from 13 backcross families that included 679 progeny....
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:Knowledge of multigenic family organization should provide insight into their mode of evolution. Accordingly, we characterized the 5S ribosomal gene family in the Drosophila melanogaster strain ry506. The 5S genes in this strain display a striking HindIII restriction difference compared to the "standard" D. melanogast...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:A BC1 population of the self-compatible tomato Lycopersicon esculentum and its wild self-incompatible relative L. hirsutum f. typicum was used for restriction fragment length polymorphism linkage analysis and quantitative trait loci (QTL) mapping of reproductive behavior and floral traits. The self-incompatibility loc...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:The walls of Aspergillus nidulans conidia contain a green pigment that protects the spores from damage by ultraviolet light. At least two genes, wA and yA, are required for pigment synthesis: yA mutants produce yellow spores, wA mutants produce white spores, and wA mutations are epistatic to yA mutations. We cloned wA...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:The variances of genetic variances within and between finite populations were systematically studied using a general multiple allele model with mutation in terms of identity by descent measures. We partitioned the genetic variances into components corresponding to genetic variances and covariances within and between l...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:A large number of arginine-requiring mutants of Neurospora was isolated, using a strain already partially impaired in an enzyme of the pathway. Among the mutants, all previously described loci, except one, were represented, and several new loci were defined and mapped. Four groups of mutants were of particular interes...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:Dispersal is a crucial factor in natural evolution, since it determines the habitat experienced by any population and defines the spatial scale of interactions between individuals. There is compelling evidence for systematic differences in dispersal characteristics within the same population, i.e., genotype-dependent ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.193946
更新日期：2017-01-01 00:00:00
abstract：:Active endogenous transposable elements, useful tools for gene isolation, have not been reported from any legume species. An active transposable element was suggested to reside in the W4 locus that governs flower color in soybean. Through biochemical and molecular analyses of several revertants of the w4-m allele, we ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.107904
更新日期：2010-01-01 00:00:00
abstract：:The fission yeast DNA repair gene rad21+ is essential for cell growth. To investigate the function essential for cell proliferation, we have isolated a temperature-sensitive mutant of the rad21+ gene. The mutant, rad21-K1, showed abnormal mitosis at the nonpermissive temperature. Some cells contained abnormal nuclear ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Resolving the mechanistic and genetic bases of reproductive barriers between species is essential to understanding the evolutionary forces that shape speciation. Intrinsic hybrid incompatibilities are often treated as fixed between species, yet there can be considerable variation in the strength of reproductive isolat...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300840
更新日期：2018-07-01 00:00:00
abstract：:Recessive mutant alleles at the autosomal dpy-21 locus of C. elegans cause a dumpy phenotype in XX animals but not in XO animals. This dumpy phenotype is characteristic of X chromosome aneuploids with higher than normal X to autosome ratios and is proposed to result from overexpression of X-linked genes. We have isola...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:To investigate the genetic basis of maize adaptation to temperate climate, collections of 375 inbred lines and 275 landraces, representative of American and European diversity, were evaluated for flowering time under short- and long-day conditions. The inbred line collection was genotyped for 55 genomewide simple sequ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.048603
更新日期：2006-04-01 00:00:00
abstract：:Pseudo-homothallism is a reproductive strategy elected by some fungi producing heterokaryotic sexual spores containing genetically different but sexually compatible nuclei. This lifestyle appears as a compromise between true homothallism (self-fertility with predominant inbreeding) and complete heterothallism (with ex...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.159988
更新日期：2014-05-01 00:00:00
abstract：:Genetic analyses of nine traits associated with stem water-soluble carbohydrate (SWSC) accumulation and remobilization at grain-filling period under drought stress (DS) and well-watered (WW) conditions were undertaken using doubled haploid lines (DHLs) derived from two Chinese common wheat cultivars. Some significantl...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.068361
更新日期：2007-05-01 00:00:00
abstract：:We recently described a yeast assay suitable for genetic screening in which simple religation nonhomologous end-joining (NHEJ) and single-strand annealing (SSA) compete for repair of an I-SceI-created double-strand break. Here, the required allele has been introduced into an array of 4781 MATa deletion mutants and eac...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
