Abstract:
:Mismatch repair of palindromic loops in the presence or absence of single-base mismatches was investigated in wild-type and mismatch-binding defective mutant Chinese hamster ovary cells. Recombination intermediates with a maximum heteroduplex DNA (hDNA) region of 697 bp contained a centrally located, phenotypically silent 12-base palindromic loop mismatch, and/or five single-base mismatches. In wild-type cells, both loops and single-base mismatches were efficiently repaired (80-100%). When no other mismatches were present in hDNA, loops were retained with a 1.6-1.9:1 bias. However, this bias was eliminated when single-base mismatches were present, perhaps because single-base mismatches signal nick-directed repair. In the multiple marker crosses, most repair tracts were long and continuous, with preferential loss of markers in cis to proximal nicks, consistent with nicks directing most repair in this situation. However, approximately 25% of repair tracts were discontinuous as a result of loop-specific repair, or from segregation or short tract repair of single-base mismatches. In mutant cells, single-base mismatches were repaired less frequently, but the loop was still repaired efficiently and with bias toward loop retention, indicating that the defect in these cells does not affect loop-specific repair. Repair tracts in products from mutant cells showed a wide variety of mosaic patterns reflecting short regions of repair and segregation consistent with reduced nick-directed repair. In mutant cells, single-base mismatches were repaired more efficiently in the presence of the loop than in its absence, a likely consequence of corepair initiated at the loop.
journal_name
Geneticsjournal_title
Geneticsauthors
Taghian DG,Hough H,Nickoloff JAsubject
Has Abstractpub_date
1998-03-01 00:00:00pages
1257-68issue
3eissn
0016-6731issn
1943-2631journal_volume
148pub_type
杂志文章相关文献
GENETICS文献大全abstract::Eight hundred second chromosomes were extracted from the Ishigakijima population, one of the southernmost populations of Drosophila melanogaster in Japan. Half of them were extracted in Native cytoplasm (P-type), and half in Foreign cytoplasm (M-type). Various population-genetic parameters, including the frequency of ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::USING A NEWLY DEVELOPED PLATING SYSTEM, WE HAVE MEASURED CELL SURVIVAL AND THE FREQUENCIES OF VARIATION IN AN INHERITED TRAIT AFTER TREATMENT OF SOYBEAN CELL SUSPENSIONS WITH DIFFERENT MUTAGENS: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-Methyl-N'-nitro-N-nitroso-guanidine (MNNG), hycanthone (1-{[2...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::There are two naturally occurring functional alleles of the recombination hotspot cog, which is located 3.5 kb from the his-3 locus of Neurospora crassa. The presence of the cog+ allele in a cross significantly increases recombination in the his-3 region compared to a cross homozygous for the cog allele. Data obtained...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.025080
更新日期:2004-07-01 00:00:00
abstract::Explanations of the evolution of diploidy have focused on the advantages gained from masking deleterious alleles. Recent theory has shown, however, that masking does not always provide an advantage to diploidy and would never favor diploidy in predominantly asexual organisms. We explore a neglected alternative theory ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Determining parentage is a fundamental problem in biology and in applications such as identifying pedigrees. Difficulties inferring parentage derive from extensive inbreeding within the population, whether natural or planned; using an insufficient number of hypervariable loci; and from allele mismatches caused by muta...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::Populations of several co-existing species of Simocephalus were examined electrophoretically at 11 enzyme loci. The prevailing pattern was one of extensive allelic substitution between species and low levels of polymorphism (0-10%) within species. Genetic identity values between species were very low (10-50%). These r...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-09-01 00:00:00
abstract::An analysis of the effects of spontaneous mutations affecting age-specific mortality was conducted using 29 lines of Drosophila melanogaster that had accumulated spontaneous mutations for 19 generations. Divergence among the lines was used to estimate the mutational variance for weekly mortality rates and the covarian...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Delta-9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F(1)'s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Cryptococcus neoformans is a fungal pathogen that causes meningitis in immunocompromised hosts. The organism has a known sexual cycle, and strains of the MATalpha mating type are more virulent than isogenic MATa strains in mice, and they are more common in the environment and infected hosts. A C. neoformans homolog of...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::This study examines the intrinsic fitness burden associated with RNA polymerase (rpoB) mutations conferring rifampin resistance in Escherichia coli K12 (MG1655) and explores the nature of adaptation to the costs of resistance. Among 28 independent Rif(r) mutants, the per-generation fitness burden (in the absence of ri...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Type II topoisomerases are essential ATP-dependent homodimeric enzymes required for transcription, replication, and chromosome segregation. These proteins alter DNA topology by generating transient enzyme-linked double-strand breaks for passage of one DNA strand through another. The central role of type II topoisomera...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.144006
更新日期:2012-11-01 00:00:00
abstract::There are two distinct reasons for making comparisons of genetic variation for quantitative characters. The first is to compare evolvabilities, or ability to respond to selection, and the second is to make inferences about the forces that maintain genetic variability. Measures of variation that are standardized by the...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::In order to examine the mechanisms of mutagenesis by a bulky DNA lesion at the guanine N7 position, the replicative form DNA of phage M13AB28 (mp8 without the amber codons in phage genes) was modified in vitro with aflatoxin B1-2,3-dichloride and transfected into appropriate Escherichia coli cells. Forward mutations i...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::A common theme in medical microbiology is that the amount of amino acid sequence variation in proteins that are targets of the host immune system greatly exceeds that found in metabolic enzymes or other housekeeping proteins. Twenty-four Mycobacterium tuberculosis genes coding for targets of the host immune system wer...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Phenoxazinone synthetase, which catalyzes the condensation of 3-hydroxykynurenine to xanthommatin, the brown eye pigment of Drosophila, is shown to exist in association with a particle which resembles the cytologically defined Type I pigment granule. Several classical eye color mutants (v, cn, st, ltd, cd, w), includi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-01-01 00:00:00
abstract::Although the role of U1 small nuclear RNAs (snRNAs) in 5' splice site recognition is well established, suppressor U1 snRNAs active in intact multicellular animals have been lacking. Here we describe suppression of a 5' splice site mutation in the Drosophila melanogaster white gene (wDR18) by compensatory changes in U1...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Adaptation in diploids is predicted to proceed via mutations that are at least partially dominant in fitness. Recently, we argued that many adaptive mutations might also be commonly overdominant in fitness. Natural (directional) selection acting on overdominant mutations should drive them into the population but then,...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.185165
更新日期:2016-07-01 00:00:00
abstract::A location of the structural gene(s) for dopa decarboxylase (EC 4.1.1.26) is proposed on the basis of enzyme determinations in a set of duplication-bearing aneuploids, which revealed only one dosage-sensitive region in the Drosophila genome. This region lies between 36EF and 37D on the left arm of chromosome 2. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Bisphenol A (BPA) is an organic compound to which human populations are ubiquitously exposed. Epidemiological data suggest BPA exposure might be associated with higher rates of diabetes and reproductive anomalies. Health concerns also include transgenerational consequences, but these mechanisms are crudely defined. Si...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.163170
更新日期:2014-05-01 00:00:00
abstract::Competition among domesticated plants or animals can have a dramatic negative impact on yield of a stand or farm. The usual quantitative genetic model ignores these competitive interactions and could result in seriously incorrect breeding decisions and acerbate animal well-being. A general solution to this problem is ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.035956
更新日期:2005-07-01 00:00:00
abstract::In fission yeast, the septation initiation network (SIN) is thought to promote cytokinesis by downstream activation of Rho1, a conserved GTPase that controls cell growth and division. Here we show that Etd1 and PP2A-Pab1, antagonistic regulators of SIN, are Rho1 regulators. Our genetic and biochemical studies indicate...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.155218
更新日期:2014-02-01 00:00:00
abstract::We have identified two Saccharomyces cerevisiae genes that, in high copy, confer resistance to Kluyveromyces lactis zymocin, an inhibitor that blocks cells in the G(1) phase of the cell cycle prior to budding and DNA replication. One gene (GRX3) encodes a glutaredoxin and is likely to act at the level of zymocin entry...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Mutation of a CCG sequence in the 5'-untranslated region of the mitochondrially encoded cytochrome b mRNA in Saccharomyces cerevisiae results in destabilization of the message and respiratory deficiency of the mutant strain. This phenotype mimics that of a mutation in the nuclear CBP1 gene. Here it is shown that overe...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.036467
更新日期:2005-11-01 00:00:00
abstract::Mitochondria of Neurospora crassa contain a cyanide-resistant alternative respiratory pathway in addition to the cytochrome pathway. The alternative oxidase is present only when electron flow through the cytochrome chain is restricted. Both genomic and cDNA copies for the alternative oxidase gene have been isolated an...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Very few mutations derived from Mutator maize lines have been studied at the molecular level. The variety of Mu elements that can induce mutations, the relative frequency of mutant induction by insertion of a given class of Mu elements or by a Mu-induced genomic rearrangement, a possible intragenic insertion site spec...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::Genes in nematode and ascidian genomes frequently occur in operons--multiple genes sharing a common promoter to generate a polycistronic primary transcript--and such genes comprise 15-20% of the coding genome for Caenorhabditis elegans and Ciona intestinalis. Recent work in nematodes has demonstrated that the identity...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.115766
更新日期:2010-06-01 00:00:00
abstract::Biology is characterized by complex interactions between phenotypes, such as recursive and simultaneous relationships between substrates and enzymes in biochemical systems. Structural equation models (SEMs) can be used to study such relationships in multivariate analyses, e.g., with multiple traits in a quantitative g...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.112979
更新日期:2010-06-01 00:00:00
abstract::This article focuses on conducting global testing for association between a binary trait and a set of rare variants (RVs), although its application can be much broader to other types of traits, common variants (CVs), and gene set or pathway analysis. We show that many of the existing tests have deteriorating performan...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.165035
更新日期:2014-08-01 00:00:00
abstract::Twelve mutations affecting nuclear migration, a major developmental phase in Schizophyllum commune, display a complex pattern of complementation and recombination. They are expressed only when a genetic factor controlling this phase of development, the B incompatibility factor, is operative. All twelve mutations are l...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::The LmR1 locus, which controls seedling resistance to the blackleg fungus Leptosphaeria maculans in the Brassica napus cultivar Shiralee, was positioned on linkage group N7. Fine genetic mapping in a population of 2500 backcross lines identified three molecular markers that cosegregated with LmR1. Additional linkage m...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.049098
更新日期:2005-12-01 00:00:00