Abstract:
:Adaptation in diploids is predicted to proceed via mutations that are at least partially dominant in fitness. Recently, we argued that many adaptive mutations might also be commonly overdominant in fitness. Natural (directional) selection acting on overdominant mutations should drive them into the population but then, instead of bringing them to fixation, should maintain them as balanced polymorphisms via heterozygote advantage. If true, this would make adaptive evolution in sexual diploids differ drastically from that of haploids. The validity of this prediction has not yet been tested experimentally. Here, we performed four replicate evolutionary experiments with diploid yeast populations (Saccharomyces cerevisiae) growing in glucose-limited continuous cultures. We sequenced 24 evolved clones and identified initial adaptive mutations in all four chemostats. The first adaptive mutations in all four chemostats were three copy number variations, all of which proved to be overdominant in fitness. The fact that fitness overdominant mutations were always the first step in independent adaptive walks supports the prediction that heterozygote advantage can arise as a common outcome of directional selection in diploids and demonstrates that overdominance of de novo adaptive mutations in diploids is not rare.
journal_name
Geneticsjournal_title
Geneticsauthors
Sellis D,Kvitek DJ,Dunn B,Sherlock G,Petrov DAdoi
10.1534/genetics.115.185165subject
Has Abstractpub_date
2016-07-01 00:00:00pages
1401-13issue
3eissn
0016-6731issn
1943-2631pii
genetics.115.185165journal_volume
203pub_type
杂志文章相关文献
GENETICS文献大全abstract::We report the identification of bang senseless (bss), a Drosophila melanogaster mutant exhibiting seizure-like behaviors, as an allele of the paralytic (para) voltage-gated Na(+) (Na(V)) channel gene. Mutants are more prone to seizure episodes than normal flies because of a lowered seizure threshold. The bss phenotype...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.123299
更新日期:2011-02-01 00:00:00
abstract::Fisher's geometric model was originally introduced to argue that complex adaptations must occur in small steps because of pleiotropic constraints. When supplemented with the assumption of additivity of mutational effects on phenotypic traits, it provides a simple mechanism for the emergence of genotypic epistasis from...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.199497
更新日期:2017-06-01 00:00:00
abstract::Cornell Control White Leghorn chicks were grown in a common environment to five weeks of age and selected for fast and slow gain in body weight from five to nine weeks of age at two temperatures, 21.1 degrees (cold) and 32.2 degrees (hot), during which time a constant 50% relative humidity was maintained. All lines we...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract::The SUF13 and SUF14 genes were identified among extragenic suppressors of +1 frameshift mutations. SUF13 is synonymous with MBF1, a single-copy nonessential gene coding for a POLII transcription factor. The suf13-1 mutation is a two-nucleotide deletion in the SUF13/MBF1 coding region. A suf13::TRP1 null mutant suppres...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.106.069963
更新日期:2007-01-01 00:00:00
abstract::Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from Drosophila to humans. Here, we...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303419
更新日期:2020-09-01 00:00:00
abstract::Selective sweeps are typically associated with a local reduction of genetic diversity around the adaptive site. However, selective sweeps can also quickly carry neutral mutations to observable population frequencies if they arise early in a sweep and hitchhike with the adaptive allele. We show that the interplay betwe...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.138461
更新日期:2012-06-01 00:00:00
abstract::The K1 killer plasmid, [KIL-k1], of Saccharomyces cerevisiae is a 1.25 x 10(6) dalton linear double-stranded RNA plasmid coding for a protein toxin and immunity to that toxin. The [KIL-sd1] plasmid is a replication-defective mutant of [KIL-k1] that depends on one of the recessive chromosomal superkiller (ski-) mutatio...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::We have precisely mapped and sequenced the three 5' exons of the Drosophila melanogaster Gld gene and have identified the start sites for transcription and translation. The first exon is composed of 335 nucleotides and does not contain any putative translation start codons. The second exon is separated from the first ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Telomere position effects on transcription (TPE, or telomeric silencing) are nucleated by association of nonhistone silencing factors with the telomere and propagated in subtelomeric regions through association of silencing factors with the specifically modified histones H3 and H4. However, the function of histone H2A...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::Fifty-eight isochromosomal lines sampled from two natural populations of Drosophila pseudoobscura in California and one from Bogota, Colombia, were examined using four-cutter restriction mapping. A 4.6-kb region of the xanthine dehydrogenase locus was probed and 66 of 135 restriction sites scored were polymorphic. Thi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract::Secondary trisomics and telotrisomics representing the 12 chromosomes of rice were isolated from the progenies of primary trisomics. A large population of each primary trisomic was grown. Plants showing variation in gross morphology compared to the primary trisomics and disomic sibs were selected and analyzed cytologi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::The complex structure and repetitive nature of eukaryotic ribosomal DNA (rDNA) is a challenge for genome assembly, thus the consequences of sequence variation in rDNA remain unexplored. However, renewed interest in the role that rDNA variation may play in diverse cellular functions, aside from ribosome production, hig...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302351
更新日期:2019-09-01 00:00:00
abstract::We have isolated and characterized deletions arising within a P transposon, P[hswa], in the presence of P transposase. P[hswa] carries white-apricot (wa) sequences, including a complete copia element, under the control of an hsp70 promoter, and resembles the original wa allele in eye color phenotype. In the presence o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::In Drosophila melanogaster, heterochromatin-induced silencing or position-effect variegation (PEV) of a reporter gene has provided insights into the properties of heterochromatin. Class I modifiers suppress PEV, and class II modifiers enhance PEV when the modifier gene is present in fewer than two doses. We have exami...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Immature spermatids from Caenorhabditis elegans are stimulated by an external activation signal to reorganize their membranes and cytoskeleton to form crawling spermatozoa. This rapid maturation, termed spermiogenesis, occurs without any new gene expression. To better understand this signal transduction pathway, we is...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Ataxia telangiectasia mutated (ATM) is a phosphatidyl-3-kinase-related protein kinase that functions as a central regulator of the DNA damage response in eukaryotic cells. In humans, mutations in ATM cause the devastating neurodegenerative disease ataxia telangiectasia. Previously, we characterized the homolog of ATM ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.080879
更新日期:2008-02-01 00:00:00
abstract::Brassica carinata, an allotetraploid with B and C genomes, has a number of traits that would be valuable to introgress into B. napus. Interspecific hybrids were created between B. carinata (BBCC) and B. napus (AACC), using an advanced backcross approach to identify and introgress traits of agronomic interest from the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.124925
更新日期:2011-03-01 00:00:00
abstract::The bacterium Myxococcus xanthus glides over surfaces using two different locomotive mechanisms, called S (social) and A (adventurous) motility that enable cells to move both as groups and as individuals. Neither mechanism involves flagella. The functions of these two motors are coordinated by the activity of a small ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.076182
更新日期:2007-09-01 00:00:00
abstract::We have examined the effects of prolonged histidine deprivation on the reversion of Salmonella typhimurium histidine auxotrophs containing either hisG46, a missense mutation (CTC----CCC), or hisG428, an ochre mutation (CAA----TAA). Both of these mutants can revert to His+ via intragenic and extragenic mechanisms. Wher...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Conditional cdc1(Ts) mutants of S. cerevisiae arrest with a phenotype similar to that exhibited by Mn(2+)-depleted cells. Sequence similarity between Cdc1p and a class of Mn(2+)-dependent phosphoesterases, as well as the observation that conditional cdc1(Ts) growth can be ameliorated by Mn(2+) supplement, suggests tha...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.167.1.107
更新日期:2004-05-01 00:00:00
abstract::The Drosophila hook gene encodes a novel component of the endocytic compartment. Previously identified hook alleles, which still expressed truncated Hook proteins, affected the accumulation of internalized transmembrane ligands into multivesicular bodies (MVBs). To determine the hook null phenotype, we isolated nine n...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::The Su(f) protein of Drosophila melanogaster shares extensive homologies with proteins from yeast (RNA14) and man (77 kD subunit of cleavage stimulation factor) that are required for 3' end processing of mRNA. These homologies suggest that su(f) is involved in mRNA 3' end formation and that some aspects of this proces...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::Eukaryotic genomes are rich in transcription units encoding "long noncoding RNAs" (lncRNAs). The purpose of all this transcription is unclear since most lncRNAs are quickly targeted for destruction during synthesis or shortly thereafter. As debates continue over the functional significance of many specific lncRNAs, su...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.117.300095
更新日期:2017-10-01 00:00:00
abstract::Two closely related bacteriophage, varphiCr30 and varphiCr35, are the first bacteriophage shown to mediate generalized transduction in Caulobacter crescentus. Unlike most other transducing phage, they are virulent and do not form any sort of lysogenic relationship with their host. However, they are rather inefficient ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1977-11-01 00:00:00
abstract::We have analyzed pregastrulation cleavage patterns in Caenorhabditis elegans embryos homozygous for various chromosomal deficiencies. By two different estimates these deficiencies represent between 37 and 49% of the genome, including the entire X chromosome and substantial portions of each of the five autosomes. Among...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::We have cloned and disrupted in vivo, by repeat-induced point mutations, the nuclear gene coding for an iron sulfur subunit of complex I from Neurospora crassa, homologue of the mammalian TYKY protein. Analysis of the obtained mutant nuo21.3c revealed that complex I fails to assemble. The peripheral arm of the enzyme ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects; however, the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes and pathways importa...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300527
更新日期:2018-02-01 00:00:00
abstract::The distribution of the number of HLA haplotypes shared by sibs affected with the same HLA-linked disease can be used to obtain information on the genetics of the disease. Since the inception of the use of sib-pair methods for the analysis of the HLA-associated diseases, the question has been raised of how to include ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::Sensory cues regulate several aspects of behavior and development in Caenorhabditis elegans, including entry into and exit from an alternative developmental stage called the dauer larva. Three parallel pathways, including a TGF-beta-like pathway, regulate dauer formation. The mechanisms by which the activities of thes...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00