Abstract：
:Ataxia telangiectasia mutated (ATM) is a phosphatidyl-3-kinase-related protein kinase that functions as a central regulator of the DNA damage response in eukaryotic cells. In humans, mutations in ATM cause the devastating neurodegenerative disease ataxia telangiectasia. Previously, we characterized the homolog of ATM (AtmA) in the filamentous fungus Aspergillus nidulans. In addition to its expected role in the DNA damage response, we found that AtmA is also required for polarized hyphal growth. Here, we extended these studies by investigating which components of the DNA damage response pathway are interacting with AtmA. The AtmA(ATM) loss of function caused synthetic lethality when combined with mutation in UvsB(ATR). Our results suggest that AtmA and UvsB are interacting and they are probably partially redundant in terms of DNA damage sensing and/or repairing and polar growth. We identified and inactivated A. nidulans chkA(CHK1) and chkB(CHK2) genes. These genes are also redundantly involved in A. nidulans DNA damage response. We constructed several combinations of double mutants for DeltaatmA, DeltauvsB, DeltachkA, and DeltachkB. We observed a complex genetic relationship with these mutations during the DNA replication checkpoint and DNA damage response. Finally, we observed epistatic and synergistic interactions between AtmA, and bimE(APC1), ankA(WEE1) and the cdc2-related kinase npkA, at S-phase checkpoint and in response to DNA-damaging agents.
journal_name
Geneticsjournal_title
Geneticsauthors
Malavazi I,Lima JF,de Castro PA,Savoldi M,de Souza Goldman MH,Goldman GHdoi
10.1534/genetics.107.080879subject
Has Abstractpub_date
2008-02-01 00:00:00pages
675-91issue
2eissn
0016-6731issn
1943-2631pii
genetics.107.080879journal_volume
178pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Site-specific recombinases are widely used tools for analysis of genetics, development, and cell biology, and many schemes have been devised to alter gene expression by recombinase-mediated DNA rearrangements. Because the FRT and lox target sites for the commonly used FLP and Cre recombinases are asymmetrical, and mus...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303394
更新日期：2020-08-01 00:00:00
abstract：:Previous work showed that C. elegans gon-14 is required for gonadogenesis. Here we report that gon-14 encodes a protein with similarity to LIN-15B, a class B synMuv protein. An extensive region of GON-14 contains blocks of sequence similarity to transposases of the hAT superfamily, but key residues are not conserved, ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.048751
更新日期：2006-02-01 00:00:00
abstract：:RNA transcribed from the Saccharomyces cerevisiae retrotransposon Ty1 accumulates to a high level in mitotically growing haploid cells, yet transposition occurs at very low frequencies. The product of reverse transcription is a linear double-stranded DNA molecule that reenters the genome by either Ty1-integrase-mediat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Vegetative incompatibility in fungi has long been known to reduce the transmission of viruses between individuals, but the barrier to transmission is incomplete. In replicated laboratory assays, we showed conclusively that the transmission of viruses between individuals of the chestnut blight fungus Cryphonectria para...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Segregation data were obtained for 1260 potato linkage group I-specific AFLP loci from a heterozygous diploid potato population. Analytical tools that identified potential typing errors and/or inconsistencies in the data and that assembled cosegregating markers into bins were applied. Bins contain multiple-marker data...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:Predicting the chromosomal location of mapped markers has been difficult because linkage maps do not reveal differences in crossover frequencies along the physical structure of chromosomes. Here we combine a physical crossover map based on the distribution of recombination nodules (RNs) on Solanum lycopersicum (tomato...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.074138
更新日期：2007-08-01 00:00:00
abstract：:One of the key factors contributing to the success of a quantitative trait locus (QTL) mapping experiment is the precision with which QTL positions can be estimated. We show, using simulations, that QTL mapping precision for an experimental cross can be increased by the use of a genotypically selected sample of indivi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.033746
更新日期：2005-05-01 00:00:00
abstract：:The idealized individual in many fungal species is a haploid self-sterile hermaphrodite that may be propagated by asexually produced spores or that may reproduce sexually. In field populations, polymorphism occurs for female-sterile/hermaphrodite status, and female-sterile mutants, which function only as males during ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:Knowledge of multigenic family organization should provide insight into their mode of evolution. Accordingly, we characterized the 5S ribosomal gene family in the Drosophila melanogaster strain ry506. The 5S genes in this strain display a striking HindIII restriction difference compared to the "standard" D. melanogast...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:Sphingolipid signaling plays an important role in the regulation of central cellular processes, including cell growth, survival, and differentiation. Many of the essential pathways responsible for sphingolipid biogenesis, and key cellular responses to changes in sphingolipid balance, are conserved between mammalian an...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.064527
更新日期：2007-01-01 00:00:00
abstract：:Genetic analysis of 131 independent transpositions of the tetracycline-resistance element Tn10 from a single site in phage P22 into the histidine operon of Salmonella typhimurium reveals that Tn10 insertions are not randomly distributed along this chromosomal target. The insertions occur in 22 different "clusters"; in...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Twelve laboratory populations of recently collected Drosophila willistoni were begun with different frequencies of alleles at three enzyme loci, six populations at 25 degrees and six at 19 degrees . Periodic sampling of the populations allowed monitoring of the frequency changes in allozymes over time.-At Lap-5 (a loc...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-11-01 00:00:00
abstract：:Acrylamide gel electrophoresis was performed on the enzyme xanthine dehydrogenase in sixty isochromosomal lines of Drosophila persimilis from three geographic populations. Sequential electrophoretic analysis using varied gel concentrations and buffers revealed twenty-three alleles in this species where only five had b...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:The contribution of epigenetic alterations to natural variation for gene transcription levels remains unclear. In this study, we investigated the functional targets of the maize chromomethylase ZMET2 in multiple inbred lines to determine whether epigenetic changes conditioned by this chromomethylase are conserved or v...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.072702
更新日期：2007-10-01 00:00:00
abstract：:Two protocols are presented that allow the selection of mutations mapping to micronuclear chromosome 5 in Tetrahymena thermophilia. One protocol involves crossing mutagenized diploid cells directly to a strain nullisomic for chromosome 5 and screening the monosomic progeny for a mutant phenotype. The second protocol f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:The genetic and environmental components of variance for two quantitative characters were measured in the descendants of Drosophila melanogaster populations which had been grown for several generations at densities of 100, 200, 300, and 400 eggs per vial. Populations subject to intermediate densities had a greater pro...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-03-01 00:00:00
abstract：:To identify the callipyge mutation, we have resequenced 184 kb spanning the DLK1-, GTL2-, PEG11-, and MEG8-imprinted domain and have identified an A-to-G transition in a highly conserved dodecamer motif between DLK1 and GTL2. This was the only difference found between the callipyge (CLPG) allele and a phylogenetically...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Age-related macular degeneration (AMD) is a leading cause of blindness in the developed world. While many AMD susceptibility variants have been identified, their influence on AMD progression has not been elucidated. Using data from two large clinical trials, Age-Related Eye Disease Study (AREDS) and AREDS2, we evaluat...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.196998
更新日期：2017-05-01 00:00:00
abstract：:Maintenance of cell integrity and cell-to-cell communication are fundamental biological processes. Filamentous fungi, such as Neurospora crassa, depend on communication to locate compatible cells, coordinate cell fusion, and establish a robust hyphal network. Two MAP kinase (MAPK) pathways are essential for communicat...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300904
更新日期：2018-06-01 00:00:00
abstract：:Several adhesins are induced by pheromones during mating in Saccharomyces cerevisiae, including Aga1p, Aga2p, Sag1p (Agalpha1p), and Fig2p. These four proteins all participate in or influence a well-studied agglutinin interaction mediated by Aga1p-Aga2p complexes and Sag1p; however, they also play redundant and essent...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.100073
更新日期：2009-05-01 00:00:00
abstract：:Four maternal-effect sterile genes, mes-2, mes-3, mes-4, and mes-6, are essential for germline development in Caenorhabditis elegans. Homozygous mes progeny from heterozygous mothers are themselves fertile but produce sterile progeny with underproliferated and degenerated germlines. All four mes genes encode chromatin...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:Drosophila adult leg development provides an ideal model system for characterizing the molecular mechanisms of hormone-triggered morphogenesis. A pulse of the steroid hormone ecdysone at the onset of metamorphosis triggers the rapid transformation of a flat leg imaginal disc into an immature adult leg, largely through...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:We examine the influence of nonrandom mating and immigration on the evolutionary dynamics of cytonuclear associations in hybrid zones. Recursion equations for allelic and genotypic cytonuclear disequilibria were generated under models of (1) migration alone, assuming hybrid zone matings are random with respect to cyto...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:Samples of mtDNA isolated from each of 92 lizards representing all color pattern classes of Cnemidophorus tesselatus and two populations of C. tigris marmoratus were digested with the restriction endonucleases MboI, TaqI, RsaI and MspI. The mtDNA fragment sizes were compared after radioactive labeling and gel electrop...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:The nuclear aod-1 gene of Neurospora crassa encodes the alternative oxidase and is induced when the standard cytochrome-mediated respiratory chain of mitochondria is inhibited. To study elements of the pathway responsible for alternative oxidase induction, we generated a series of mutations in the region upstream from...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.068635
更新日期：2007-04-01 00:00:00
abstract：:A new suppressor of +1 frameshift mutations has been isolated in Salmonella typhimurium. This suppressor, sufJ, maps at minute 89 on the Salmonella genetic map between the argH and rpo(rif) loci, closely linked to the gene for the ochre suppressor tyrU(supM). The suppressor mutation is dominant to its wild-type allele...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Differential gene expression across cell types underlies development and cell physiology in multicellular organisms. Caenorhabditis elegans is a powerful, extensively used model to address these biological questions. A remaining bottleneck relates to the difficulty to obtain comprehensive tissue-specific gene transcri...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303774
更新日期：2020-12-01 00:00:00
abstract：:The Podospora anserina PaMpk1 MAP kinase (MAPK) signaling pathway can generate a cytoplasmic and infectious element resembling prions. When present in the cells, this C element causes the crippled growth (CG) cell degeneration. CG results from the inappropriate autocatalytic activation of the PaMpk1 MAPK pathway durin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.139469
更新日期：2012-06-01 00:00:00
abstract：:The mak7-1 mutant loses the killer toxin-encoding M1 dsRNA. MAK7 is RPL4A, one of two genes encoding ribosomal protein L4. KRB1 is a dominant suppressor of mak7-1 that is tightly centromerelinked, but not linked to centromere markers of chromosomes I-XVI. Our orthogonal field agarose gel electrophoresis analysis of ch...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:The yeast genetics community has embraced genomic biology, and there is a general understanding that obtaining a full encyclopedia of functions of the approximately 6000 genes is a worthwhile goal. The yeast literature comprises over 40,000 research papers, and the number of yeast researchers exceeds the number of gen...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.107.074468
更新日期：2007-05-01 00:00:00
