Abstract：
:Two protocols are presented that allow the selection of mutations mapping to micronuclear chromosome 5 in Tetrahymena thermophilia. One protocol involves crossing mutagenized diploid cells directly to a strain nullisomic for chromosome 5 and screening the monosomic progeny for a mutant phenotype. The second protocol first takes the mutagenized diploid cells through round I of genomic exclusion to create useful and reusable mutant heterokaryons, which are then assayed for the presence of mutations on chromosome 5 by crossing to the nullisomic 5 strain. Of 14 putative chromosome 5 mutations obtained by these two methods, seven are shown by genetic analysis to be recessive mutations on chromosome 5; one mapped elsewhere in the genome; six were infertile or failed to yield progeny in some of the diagnostic crosses and, thus, their genetic nature could not be determined with certainty.
journal_name
Geneticsjournal_title
Geneticsauthors
Altschuler MI,Bruns PJsubject
Has Abstractpub_date
1984-03-01 00:00:00pages
387-401issue
3eissn
0016-6731issn
1943-2631journal_volume
106pub_type
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