Abstract:
:The nuclear aod-1 gene of Neurospora crassa encodes the alternative oxidase and is induced when the standard cytochrome-mediated respiratory chain of mitochondria is inhibited. To study elements of the pathway responsible for alternative oxidase induction, we generated a series of mutations in the region upstream from the aod-1 structural gene and transformed the constructs into an aod-1 mutant strain. Transformed conidia were plated on media containing antimycin A, which inhibits the cytochrome-mediated electron transport chain so that only cells expressing alternative oxidase will grow. Using this functional in vivo assay, we identified an alternative oxidase induction motif (AIM) that is required for efficient expression of aod-1. The AIM sequence consists of two CGG repeats separated by 7 bp and is similar to sequences known to be bound by members of the Zn(II)2Cys6 binuclear cluster family of transcription factors. The AIM motif appears to be conserved in other species found in the order Sordariales.
journal_name
Geneticsjournal_title
Geneticsauthors
Chae MS,Lin CC,Kessler KE,Nargang CE,Tanton LL,Hahn LB,Nargang FEdoi
10.1534/genetics.106.068635subject
Has Abstractpub_date
2007-04-01 00:00:00pages
1597-606issue
4eissn
0016-6731issn
1943-2631pii
genetics.106.068635journal_volume
175pub_type
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