Regulation of Cell-to-Cell Communication and Cell Wall Integrity by a Network of MAP Kinase Pathways and Transcription Factors in Neurospora crassa.

Abstract:

:Maintenance of cell integrity and cell-to-cell communication are fundamental biological processes. Filamentous fungi, such as Neurospora crassa, depend on communication to locate compatible cells, coordinate cell fusion, and establish a robust hyphal network. Two MAP kinase (MAPK) pathways are essential for communication and cell fusion in N. crassa: the cell wall integrity/MAK-1 pathway and the MAK-2 (signal response) pathway. Previous studies have demonstrated several points of cross-talk between the MAK-1 and MAK-2 pathways, which is likely necessary for coordinating chemotropic growth toward an extracellular signal, and then mediating cell fusion. Canonical MAPK pathways begin with signal reception and end with a transcriptional response. Two transcription factors, ADV-1 and PP-1, are essential for communication and cell fusion. PP-1 is the conserved target of MAK-2, but it is unclear what targets ADV-1. We did RNA sequencing on Δadv-1, Δpp-1, and wild-type cells and found that ADV-1 and PP-1 have a shared regulon including many genes required for communication, cell fusion, growth, development, and stress response. We identified ADV-1 and PP-1 binding sites across the genome by adapting the in vitro method of DNA-affinity purification sequencing for N. crassa To elucidate the regulatory network, we misexpressed each transcription factor in each upstream MAPK deletion mutant. Misexpression of adv-1 was sufficient to fully suppress the phenotype of the Δpp-1 mutant and partially suppress the phenotype of the Δmak-1 mutant. Collectively, our data demonstrate that the MAK-1/ADV-1 and MAK-2/PP-1 pathways form a tight regulatory network that maintains cell integrity and mediates communication and cell fusion.

journal_name

Genetics

journal_title

Genetics

authors

Fischer MS,Wu VW,Lee JE,O'Malley RC,Glass NL

doi

10.1534/genetics.118.300904

subject

Has Abstract

pub_date

2018-06-01 00:00:00

pages

489-506

issue

2

eissn

0016-6731

issn

1943-2631

pii

genetics.118.300904

journal_volume

209

pub_type

杂志文章

相关文献

GENETICS文献大全
  • "Wrecks of Ancient Life": Genetic Variants Vetted by Natural Selection.

    abstract::The Genetics Society of America's George W. Beadle Award honors individuals who have made outstanding contributions to the community of genetics researchers and who exemplify the qualities of its namesake as a respected academic, administrator, and public servant. The 2015 recipient is John Postlethwait. He has made g...

    journal_title:Genetics

    pub_type: 历史文章,杂志文章

    doi:10.1534/genetics.115.178442

    authors: Postlethwait JH

    更新日期:2015-07-01 00:00:00

  • Analysis of B-genome chromosome introgression in interspecific hybrids of Brassica napus × B. carinata.

    abstract::Brassica carinata, an allotetraploid with B and C genomes, has a number of traits that would be valuable to introgress into B. napus. Interspecific hybrids were created between B. carinata (BBCC) and B. napus (AACC), using an advanced backcross approach to identify and introgress traits of agronomic interest from the ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.110.124925

    authors: Navabi ZK,Stead KE,Pires JC,Xiong Z,Sharpe AG,Parkin IA,Rahman MH,Good AG

    更新日期:2011-03-01 00:00:00

  • Novel Innate Immune Genes Regulating the Macrophage Response to Gram Positive Bacteria.

    abstract::Host variation in Toll-like receptors and other innate immune signaling molecules alters infection susceptibility. However, only a portion of the variability observed in the innate immune response is accounted for by known genes in these pathways. Thus, the identification of additional genes that regulate the response...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.115.185314

    authors: Alper S,Warg LA,De Arras L,Flatley BR,Davidson EJ,Adams J,Smith K,Wohlford-Lenane CL,McCray PB Jr,Pedersen BS,Schwartz DA,Yang IV

    更新日期:2016-09-01 00:00:00

  • Beyond Thermodynamic Constraints: Evolutionary Sampling Generates Realistic Protein Sequence Variation.

    abstract::Biological evolution generates a surprising amount of site-specific variability in protein sequences. Yet, attempts at modeling this process have been only moderately successful, and current models based on protein structural metrics explain, at best, 60% of the observed variation. Surprisingly, simple measures of pro...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.118.300699

    authors: Jiang Q,Teufel AI,Jackson EL,Wilke CO

    更新日期:2018-04-01 00:00:00

  • Epistatic control of non-Mendelian inheritance in mouse interspecific crosses.

    abstract::Strong deviation of allele frequencies from Mendelian inheritance favoring Mus spretus-derived alleles has been described previously for X-linked loci in four mouse interspecific crosses. We reanalyzed data for three of these crosses focusing on the location of the gene(s) controlling deviation on the X chromosome and...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Montagutelli X,Turner R,Nadeau JH

    更新日期:1996-08-01 00:00:00

  • Logjam encodes a predicted EMP24/GP25 protein that is required for Drosophila oviposition behavior.

    abstract::A newly characterized Drosophila melanogaster gene, logjam (loj), functions in female reproduction by modulating oviposition behavior. The locus encodes at least six overlapping transcripts with unique 5' ends. P-element mutants that express very low levels of loj transcripts are unable to oviposit mature eggs. This p...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Carney GE,Taylor BJ

    更新日期:2003-05-01 00:00:00

  • Selective genotyping and phenotyping strategies in a complex trait context.

    abstract::Selective genotyping and phenotyping strategies are used to lower the cost of quantitative trait locus studies. Their efficiency has been studied primarily in simplified contexts--when a single locus contributes to the phenotype, and when the residual error (phenotype conditional on the genotype) is normally distribut...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.108.094607

    authors: Sen S,Johannes F,Broman KW

    更新日期:2009-04-01 00:00:00

  • The genetic basis of rapidly evolving male genital morphology in Drosophila.

    abstract::The external genitalia are some of the most rapidly evolving morphological structures in insects. The posterior lobe of the male genital arch shows striking differences in both size and shape among closely related species of the Drosophila melanogaster species subgroup. Here, we dissect the genetic basis of posterior ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.111.130815

    authors: Masly JP,Dalton JE,Srivastava S,Chen L,Arbeitman MN

    更新日期:2011-09-01 00:00:00

  • Genetic analysis of the Drosophila alphaPS2 integrin subunit reveals discrete adhesive, morphogenetic and sarcomeric functions.

    abstract::The integrin family of cell surface receptors mediates cell-substrate and cell-to-cell adhesion and transmits intracellular signals. In Drosophila there is good evidence for an adhesive role of integrins, but evidence for integrin signalling has remained elusive. Each integrin is an alphabeta heterodimer, and the Dros...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Bloor JW,Brown NH

    更新日期:1998-03-01 00:00:00

  • UGA suppressors in Saccharomyces cerevisiae: allelism, action spectra and map positions.

    abstract::Sixty independent UGA suppressors of Saccharomyces cerevisiae have been studied. They are dominant and are divided into 16 groups (loci) by recombination. Suppressors representing these loci are divided into two classes by action spectra; four in class 1 (a broad action spectrum) and 12 in class 2 (a narrow action spe...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Ono BI,Fujimoto R,Ohno Y,Maeda N,Tsuchiya Y,Usui T,Ishino-Arao Y

    更新日期:1988-01-01 00:00:00

  • Excursions along the Interface between Disruptive and Stabilizing Selection.

    abstract::When a polygenic character is exposed to natural selection in which the curve giving fitness as a function of phenotype is a mixture of two Gaussian (normal) curves, the population may respond either by evolving to a specialized phenotype near one of the two optimum phenotypes, or by evolving to a generalized phenotyp...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Felsenstein J

    更新日期:1979-11-01 00:00:00

  • Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster: characterization of male-sterile mutants generated by single P element mutagenesis.

    abstract::We describe 83 recessive autosomal male-sterile mutations, generated by single P element mutagenesis in Drosophila melanogaster. Each mutation has been localized to a lettered subdivision of the polytene map. Reversion analyses, as well as complementation tests using available chromosomal deficiencies, indicate that t...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Castrillon DH,Gönczy P,Alexander S,Rawson R,Eberhart CG,Viswanathan S,DiNardo S,Wasserman SA

    更新日期:1993-10-01 00:00:00

  • Stable Patterns of CENH3 Occupancy Through Maize Lineages Containing Genetically Similar Centromeres.

    abstract::While the approximate chromosomal position of centromeres has been identified in many species, little is known about the dynamics and diversity of centromere positions within species. Multiple lines of evidence indicate that DNA sequence has little or no impact in specifying centromeres in maize and in most multicellu...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.115.177360

    authors: Gent JI,Wang K,Jiang J,Dawe RK

    更新日期:2015-08-01 00:00:00

  • Interaction between the oxa1 and rmp1 genes modulates respiratory complex assembly and life span in Podospora anserina.

    abstract::A causal link between deficiency of the cytochrome respiratory pathway and life span was previously shown in the filamentous fungus Podospora anserina. To gain more insight into the relationship between mitochondrial function and life span, we have constructed a strain carrying a thermosensitive mutation of the gene o...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.033837

    authors: Sellem CH,Lemaire C,Lorin S,Dujardin G,Sainsard-Chanet A

    更新日期:2005-03-01 00:00:00

  • Heritability of nestling growth in cross-fostered European Starlings Sturnus vulgaris.

    abstract::In altricial birds, growth rates and nestling morphology vary between broods. For natural selection to produce evolutionary change in these variables, ther must exist heritable variation. Since nestling traits are not any longer presents in parents, traditional offspring-parent regressions cannot estimate heritabiliti...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Smith HG,Wettermark KJ

    更新日期:1995-10-01 00:00:00

  • Effects of pleiotropy on predictions concerning mutation-selection balance for polygenic traits.

    abstract::Previous mathematical analyses of mutation-selection balance for metric traits assume that selection acts on the relevant loci only through the character(s) under study. Thus, they implicitly assume that all of the relevant mutation and selection parameters are estimable. A more realistic analysis must recognize that ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Turelli M

    更新日期:1985-09-01 00:00:00

  • Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

    abstract::Genome stability in fission yeast requires the conserved S-phase kinase Hsk1 (Cdc7) and its partner Dfp1 (Dbf4). In addition to their established function in the initiation of DNA replication, we show that these proteins are important in maintaining genome integrity later in S phase and G2. hsk1 cells suffer increased...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.109.112284

    authors: Dolan WP,Le AH,Schmidt H,Yuan JP,Green M,Forsburg SL

    更新日期:2010-05-01 00:00:00

  • Unique and Overlapping Functions of Formins Frl and DAAM During Ommatidial Rotation and Neuronal Development in Drosophila.

    abstract::The noncanonical Frizzled/planar cell polarity (PCP) pathway regulates establishment of polarity within the plane of an epithelium to generate diversity of cell fates, asymmetric, but highly aligned structures, or to orchestrate the directional migration of cells during convergent extension during vertebrate gastrulat...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.115.181438

    authors: Dollar G,Gombos R,Barnett AA,Sanchez Hernandez D,Maung SM,Mihály J,Jenny A

    更新日期:2016-03-01 00:00:00

  • P-Element insertion at the polyhomeotic gene leads to formation of a novel chimeric protein that negatively regulates yellow gene expression in P-element-induced alleles of Drosophila melanogaster.

    abstract::Polyhomeotic is a member of the Polycomb group (Pc-G) of homeotic repressors. The proteins encoded by the Pc-G genes form repressive complexes on the polycomb group response element sites. The phP1 mutation was induced by insertion of a 1.2-kb P element into the 5' transcribed nontranslated region of the proximal poly...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Belenkaya T,Soldatov A,Nabirochkina E,Biryukova I,Georgieva S,Georgiev P

    更新日期:1998-10-01 00:00:00

  • Drosophila calmodulin mutants with specific defects in the musculature or in the nervous system.

    abstract::We have studied lethal mutations in the single calmodulin gene (Cam) of Drosophila to gain insight into the in vivo functions of this important calcium sensor. As a result of maternal calmodulin (CaM) in the mature egg, lethality is delayed until the postembryonic stages. Prior to death in the first larval instar, Cam...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Wang B,Sullivan KM,Beckingham K

    更新日期:2003-11-01 00:00:00

  • Stochastic demography and the neutral substitution rate in class-structured populations.

    abstract::The neutral rate of allelic substitution is analyzed for a class-structured population subject to a stationary stochastic demographic process. The substitution rate is shown to be generally equal to the effective mutation rate, and under overlapping generations it can be expressed as the effective mutation rate in new...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.114.163345

    authors: Lehmann L

    更新日期:2014-05-01 00:00:00

  • Notch and the awesome power of genetics.

    abstract::Notch is a receptor that mediates cell-cell interactions in animal development, and aberrations in Notch signal transduction can cause cancer and other human diseases. Here, I describe the major advances in the Notch field from the identification of the first mutant in Drosophila almost a century ago through the eluci...

    journal_title:Genetics

    pub_type: 杂志文章,评审

    doi:10.1534/genetics.112.141812

    authors: Greenwald I

    更新日期:2012-07-01 00:00:00

  • A simple formula useful for positional cloning.

    abstract::We derive a formula for the distribution of the length T of the recombination interval containing a target gene and using N gametes in a region where R kilobases correspond to 1 cM. The formula can be used to calculate the number of meiotic events required to narrow a target gene down to a specific interval size and h...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Durrett RT,Chen KY,Tanksley SD

    更新日期:2002-01-01 00:00:00

  • Recombination in the Vicinity of Insertions of Transposon Tn 5 in MYXOCOCCUS XANTHUS.

    abstract::To test genetic recombination in the vicinity of insertions of the transposon Tn5, crosses were performed by transduction between M. xanthus strains carrying different insertions of Tn5. One member of each pair carried resistance to kanamycin (Tn5-Km); the other carried resistance to tetracycline (Tn5-Tc). The distanc...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Sodergren E,Cheng Y,Avery L,Kaiser D

    更新日期:1983-10-01 00:00:00

  • The EGL-4 PKG acts with KIN-29 salt-inducible kinase and protein kinase A to regulate chemoreceptor gene expression and sensory behaviors in Caenorhabditis elegans.

    abstract::The regulation of chemoreceptor (CR) gene expression by environmental signals and internal cues may contribute to the modulation of multiple physiological processes and behavior in Caenorhabditis elegans. We previously showed that KIN-29, a homolog of salt-inducible kinase, acts in sensory neurons to regulate the expr...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.108.094771

    authors: van der Linden AM,Wiener S,You YJ,Kim K,Avery L,Sengupta P

    更新日期:2008-11-01 00:00:00

  • The evolution and consequences of sex-specific reproductive variance.

    abstract::Natural selection favors alleles that increase the number of offspring produced by their carriers. But in a world that is inherently uncertain within generations, selection also favors alleles that reduce the variance in the number of offspring produced. If previous studies have established this principle, they have l...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.113.156067

    authors: Mullon C,Reuter M,Lehmann L

    更新日期:2014-01-01 00:00:00

  • Inbreeding in populations with overlapping generations.

    abstract::An inbreeding matrix is defined for populations with overlapping generations. In the short term it can be expressed in terms of a matrix specifying the passage of genes between the different age groups (and sexes) and a diagonal matrix whose elements depend on the number of individuals in each age group. Formulae for ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Johnson DL

    更新日期:1977-11-01 00:00:00

  • Local PCA Shows How the Effect of Population Structure Differs Along the Genome.

    abstract::Population structure leads to systematic patterns in measures of mean relatedness between individuals in large genomic data sets, which are often discovered and visualized using dimension reduction techniques such as principal component analysis (PCA). Mean relatedness is an average of the relationships across locus-s...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.118.301747

    authors: Li H,Ralph P

    更新日期:2019-01-01 00:00:00

  • The P-3 and EST loci in the honeybee Apis mellifera.

    abstract::Data for Apis mellifera indicate that the P-3 proteins and one esterase enzyme are controlled by two genes, P-3 and Est, with two alleles each. The frequency of the P-3 alleles is different in the two subspecies (adansonii and ligustica), that for P-3(F) in Italian bees being 46.9% and in African 0.5%. The frequency o...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Mestriner MA,Contel EP

    更新日期:1972-12-01 00:00:00

  • Donor-Recipient Identification in Para- and Poly-phyletic Trees Under Alternative HIV-1 Transmission Hypotheses Using Approximate Bayesian Computation.

    abstract::Diversity of the founding population of Human Immunodeficiency Virus Type 1 (HIV-1) transmissions raises many important biological, clinical, and epidemiological issues. In up to 40% of sexual infections, there is clear evidence for multiple founding variants, which can influence the efficacy of putative prevention me...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.117.300284

    authors: Romero-Severson EO,Bulla I,Hengartner N,Bártolo I,Abecasis A,Azevedo-Pereira JM,Taveira N,Leitner T

    更新日期:2017-11-01 00:00:00