Abstract：
:RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the approach. Further, to demonstrate the versatility of the technology, we provide examples of the various applications of the method to problems in signal transduction and cell and developmental biology. Finally, we discuss emerging technological advances that will extend RNAi-based screening methods.
journal_name
Geneticsjournal_title
Geneticsauthors
Perrimon N,Mathey-Prevot Bdoi
10.1534/genetics.106.069963subject
Has Abstractpub_date
2007-01-01 00:00:00pages
7-16issue
1eissn
0016-6731issn
1943-2631pii
175/1/7journal_volume
175pub_type
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