Abstract:
:The bacterium Myxococcus xanthus glides over surfaces using two different locomotive mechanisms, called S (social) and A (adventurous) motility that enable cells to move both as groups and as individuals. Neither mechanism involves flagella. The functions of these two motors are coordinated by the activity of a small Ras-like protein, encoded by the mglA gene. The results of previous studies of a second-site suppressor of the mglA-8 missense mutation masK-815 indicate that MglA interacts with a protein tyrosine kinase, MasK, to control social motility. Sequence analysis of the sites of 12 independent insertions of the transposon magellan-4 that result in the loss of motility in an M. xanthus mglA-8 masK-815 double mutant shows that nine of these 12 insertions are in genes known to be required for S gliding motility. This result confirms that the masK-815 suppressor restores S but not A motility. Three of the 12 insertions define three new genes required for S motility and show that the attachment of heptose to the lipopolysaccharide inner core, an ortholog of the CheR methyltransferase, and a large protein with YD repeat motifs, are required for S motility. When these three insertions are backcrossed into an otherwise wild-type genetic background, their recombinants are found to have defects in S, but not, A motility. The spectrum of magellan-4 insertions that lead to the loss of S motility in the mglA-8 masK-815 double mutant background is different than that resulting from a previous mutant hunt starting with a different (A mutant) genetic background, suggesting that the number of genes required for S motility in M. xanthus is quite large.
journal_name
Geneticsjournal_title
Geneticsauthors
Youderian P,Hartzell PLdoi
10.1534/genetics.107.076182subject
Has Abstractpub_date
2007-09-01 00:00:00pages
557-66issue
1eissn
0016-6731issn
1943-2631pii
genetics.107.076182journal_volume
177pub_type
杂志文章相关文献
GENETICS文献大全abstract::The Pif1 family of DNA helicases is conserved from yeast to humans. Although the helicase domains of family members are well conserved, the amino termini of these proteins are not. The Saccharomyces cerevisiae genome encodes two Pif1 family members, Rrm3p and Pif1p, that have very different functions. To determine if ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.028035
更新日期:2004-11-01 00:00:00
abstract::Next generation sequencing (NGS) is about to revolutionize genetic analysis. Currently NGS techniques are mainly used to sequence individual genomes. Due to the high sequence coverage required, the costs for population-scale analyses are still too high to allow an extension to nonmodel organisms. Here, we show that NG...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.114397
更新日期:2010-09-01 00:00:00
abstract::The primary structures of peptides may be adapted for efficient synthesis as well as proper function. Here, the Saccharomyces cerevisiae genome sequence, DNA microarray expression data, tRNA gene numbers, and functional categorizations of proteins are employed to determine whether the amino acid composition of peptide...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::We have used a novel method to activate the DNA damage S-phase checkpoint response in Saccharomyces cerevisiae to slow lagging-strand DNA replication by exposing cells expressing a drug-sensitive DNA polymerase δ (L612M-DNA pol δ) to the inhibitory drug phosphonoacetic acid (PAA). PAA-treated pol3-L612M cells arrest a...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.131938
更新日期:2011-12-01 00:00:00
abstract::We consider some practical statistical issues in QTL analysis where several crosses originate in multiple inbred parents. Our results show that ignoring background polygenic variation in different crosses may lead to biased interval mapping estimates of QTL effects or loss of efficiency. Threshold and power approximat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract::The segregation and random assortment of characters observed by Mendel have their basis in the behavior of chromosomes in meiosis. But showing this actually to be the case requires a correct understanding of the meiotic behavior of chromosomes. This was achieved only gradually, over several decades, with much dispute ...
journal_title:Genetics
pub_type: 传,历史文章,杂志文章
doi:10.1534/genetics.112.139733
更新日期:2012-06-01 00:00:00
abstract::Argonaute2 (Ago2) is a rapidly evolving nuclease in the Drosophila melanogaster RNA interference (RNAi) pathway that targets viruses and transposable elements in somatic tissues. Here we reconstruct the history of Ago2 duplications across the D. obscura group and use patterns of gene expression to infer new functional...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.192336
更新日期:2016-10-01 00:00:00
abstract::We studied the control of pharyngeal excitation in Caenorhabditis elegans. By laser ablating subsets of the pharyngeal nervous system, we found that the MC neuron type is necessary and probably sufficient for rapid pharyngeal pumping. Electropharyngeograms showed that MC transmits excitatory postsynaptic potentials, s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::137Cs-gamma irradiation of bacteriophage T4 induces large deletions plus a variety of types of point mutations. All mutations arise with single-hit kinetics, and all by a misrepair process. The estimated point mutation rate is 1.5 X 10(-9) per locus per rad. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract::We have analyzed the 2E1-3A1 area of the X chromosome with special attention to loci related to embryogenesis. Published maps indicate that this chromosomal segment contains ten bands. Our genetic analysis has identified 11 complementation groups: one recessive visible (prune), two female steriles and eight lethals. O...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::A spontaneous lethal mutation rate approximately twenty to thirty times greater than normal has been discovered in second and third chromosomes derived from an irradiated isogenic line and paired with marked inversion chromosomes. Mutations resulting in reductions of viability of varying magnitude short of complete le...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-09-01 00:00:00
abstract::Self-incompatibility (SI) is a genetic system found in some hermaphrodite plants. Recognition of pollen by pistils expressing cognate specificities at two linked genes leads to rejection of self pollen and pollen from close relatives, i.e., to avoidance of self-fertilization and inbred matings, and thus increased outc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.127399
更新日期:2011-07-01 00:00:00
abstract::A simple two-locus gene conversion model is considered to investigate the amounts of DNA variation and linkage disequilibrium in small multigene families. The exact solutions for the expectations and variances of the amounts of variation within and between two loci are obtained. It is shown that gene conversion increa...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::There is an increasing awareness that selection affecting linked neutral sites strongly influences on how diversity is distributed across the genome. In particular, linked selection is likely involved in the formation of heterogenous landscapes of genetic diversity, including genomic regions with locally reduced effec...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.301991
更新日期:2019-05-01 00:00:00
abstract::C. elegans spermatogenesis employs lysosome-related fibrous body-membranous organelles (FB-MOs) for transport of many cellular components. Previous work showed that spe-10 mutants contain FB-MOs that prematurely disassemble, resulting in defective transport of FB components into developing spermatids. Consequently, sp...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.047340
更新日期:2006-01-01 00:00:00
abstract::To understand long terminal repeat (LTR)-retrotransposon copy number dynamics, Ty1 elements were reintroduced into a "Ty-less" Saccharomyces strain where elements had been lost by LTR-LTR recombination. Repopulated strains exhibited alterations in chromosome size that were associated with Ty1 insertions, but did not b...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.037317
更新日期:2005-04-01 00:00:00
abstract::Seed component structures were grouped into maternal and offspring (embryo and endosperm) tissues to characterize a dormancy quantitative trait locus (QTL) for tissue-specific function using a marker-assisted genetic approach. The approach was devised to test if genotypic/allelic frequencies of a marker tightly linked...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.092007
更新日期:2008-08-01 00:00:00
abstract::Previous work showed that C. elegans gon-14 is required for gonadogenesis. Here we report that gon-14 encodes a protein with similarity to LIN-15B, a class B synMuv protein. An extensive region of GON-14 contains blocks of sequence similarity to transposases of the hAT superfamily, but key residues are not conserved, ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.048751
更新日期:2006-02-01 00:00:00
abstract::For most common diseases with heritable components, not a single or a few single-nucleotide polymorphisms (SNPs) explain most of the variance for these disorders. Instead, much of the variance may be caused by interactions (epistasis) among multiple SNPs or interactions with environmental conditions. We present a new ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.071696
更新日期:2007-06-01 00:00:00
abstract::Heterochromatin protein 1a (HP1a) is a highly conserved and versatile epigenetic factor that can both silence and activate transcription. However, the function of HP1a in development has been underinvestigated. Here, we report the role of maternal HP1a in producing maternal transcripts that drive early Drosophila embr...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301704
更新日期:2019-01-01 00:00:00
abstract::The degree to which genomic architecture varies across space and time is central to the evolution of genomes in response to natural selection. Bulked-segregant mapping combined with pooled sequencing provides an efficient means to estimate the effect of genetic variants on quantitative traits. We develop a novel likel...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.201483
更新日期:2017-07-01 00:00:00
abstract::In altricial birds, growth rates and nestling morphology vary between broods. For natural selection to produce evolutionary change in these variables, ther must exist heritable variation. Since nestling traits are not any longer presents in parents, traditional offspring-parent regressions cannot estimate heritabiliti...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::Haplotype variation in 9.7 kb of genomic DNA sequence from the human lipoprotein lipase (LPL) gene was scored in three populations: African-Americans from Jackson, Mississippi (24 individuals), Finns from North Karelia, Finland (24), and non-Hispanic whites from Rochester, Minnesota (23). Earlier analyses had indicate...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::We developed a screening approach that utilizes an inverse polymerase chain reaction (PCR) to detect P element insertions in or near previously cloned genes in Drosophila melanogaster. We used this approach in a large scale genetic screen in which P elements were mobilized from sites on the X chromosome to new autosom...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::While bacteria divide clonally, horizontal gene transfer followed by homologous recombination is now recognized as an important contributor to their evolution. However, the details of how the competition between clonality and recombination shapes genome diversity remains poorly understood. Using a computational model,...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300061
更新日期:2017-09-01 00:00:00
abstract::The forest tent caterpillar is polymorphic for two melanic genes affecting wing color of moths. These are the first genetically determined morphological traits reported for the genus. Dark (D) is a sex-limited, autosomal dominant with a phenotype of dark brown males. Frequencies in population samples varied from 8 to ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Mitochondrial DNAs (mtDNAs) from 167 American Indians including 87 Amerind-speakers (Amerinds) and 80 Nadene-speakers (Nadene) were surveyed for sequence variation by detailed restriction analysis. All Native American mtDNAs clustered into one of four distinct lineages, defined by the restriction site variants: HincII...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::To study the evolutionary role played by differential male and female fertility (sexual asymmetry) both between individuals and over the life span within single individuals, the terms "intrinsic male fertility" and "intrinsic female fertility" are introduced. With the help of these terms, the concept of sexual asymmet...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::Expectations for the time scale and structure of allelic genealogies in finite populations are formed under three models of sporophytic self-incompatibility. The models differ in the dominance interactions among the alleles that determine the self-incompatibility phenotype: In the SSIcod model, alleles act codominantl...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::The reproductive success of individual fire ant queens (Solenopsis invicta) previously has been shown to be strongly influenced by their genotype at a single enzyme-encoding gene, designated Pgm-3. This paper presents evidence that a second, tightly linked gene, designated Gp-9, is under similarly strong selection in ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00