Abstract：
:Conditional cdc1(Ts) mutants of S. cerevisiae arrest with a phenotype similar to that exhibited by Mn(2+)-depleted cells. Sequence similarity between Cdc1p and a class of Mn(2+)-dependent phosphoesterases, as well as the observation that conditional cdc1(Ts) growth can be ameliorated by Mn(2+) supplement, suggests that Cdc1p activity is sensitive to intracellular Mn(2+) levels. This article identifies several previously uncharacterized cdc1(Ts) suppressors as class E vps (vacuolar protein sorting) mutants and shows that these, as well as other vps mutants, accumulate high levels of intracellular Mn(2+). Yeast VPS genes play a role in delivery of membrane transporters to the vacuole for degradation, and we show that the vps mutants accumulate elevated levels of the high-affinity Mn(2+) transporter Smf1p. cdc1(Ts) conditional growth is also alleviated by mutations, including doa4 and ubc4, that compromise protein ubiquitination, and these ubiquitination defects are associated with Smf1p accumulation. Epistasis studies show that these suppressors require functional Smf1p to alleviate the cdc1(Ts) growth defect, whereas Smf1p is dispensable for cdc1(Ts) suppression by a mutation (cos16/per1) that does not influence intracellular Mn(2+) levels. Because Smf1p is ubiquitinated in vivo, we propose that Smf1p is targeted to the vacuole for degradation by ubiquitination-dependent protein sorting.
journal_name
Geneticsjournal_title
Geneticsauthors
Eguez L,Chung YS,Kuchibhatla A,Paidhungat M,Garrett Sdoi
10.1534/genetics.167.1.107keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
107-17issue
1eissn
0016-6731issn
1943-2631pii
167/1/107journal_volume
167pub_type
杂志文章相关文献
GENETICS文献大全abstract：:We have characterized tandem duplications in the rII regions of phage T4. The rII deletion r1589 blocks only the function of the rIIA cistron, although it extends into the B cistron. Another rII deletion, r1236, blocks the function of the rIIB cistron and overlaps r1589. When a cross is made between r1589 and r1236, t...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:Heterologous yeast artificial chromosomes (YACs) do not recombine with each other and missegregate in 25% of meiosis I events. Recombination hot spots in the yeast Saccharomyces cerevisiae have previously been shown to be associated with sites of meiosis-induced double-strand breaks (DSBs). A 6-kb fragment containing ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:The Saccharomyces cerevisiae SEN1 gene codes for a nuclear-localized superfamily I helicase. SEN1 is an ortholog of human SETX (senataxin), which has been implicated in the neurological disorders ataxia-ocular apraxia type 2 and juvenile amyotrophic lateral sclerosis. Pleiotropic phenotypes conferred by sen1 mutations...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.110031
更新日期：2010-01-01 00:00:00
abstract：:A two-locus model is used to analyze the effectiveness of a mixture of insecticides in delaying resistance, compared to the use of the insecticides singly. The effects of factors such as recombination, effective dominance, initial value of allele frequencies and initial value of linkage disequilibrium are considered. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:To study the interdependence of Gcr1p and Rap1p, we prepared a series of synthetic regulatory sequences that contained various numbers and combinations of CT-boxes (Gcr1p-binding sites) and RPG-boxes (Rap1p-binding sites). The ability of the synthetic oligonucleotides to function as regulatory sequences was tested usi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:We have tested the hypothesis that genetic differences among conspecific populations may result in diverse responses to selection, using natural populations of Drosophila melanogaster. Selection for ethanol tolerance in a tube measuring knockdown resistance was imposed on five West Coast populations. In 24 generations...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:The essential neurotransmitter acetylcholine functions throughout the animal kingdom. In Caenorhabditis elegans, the acetylcholine biosynthetic enzyme [choline acetyltransferase (ChAT)] and vesicular transporter [vesicular acetylcholine transporter (VAChT)] are encoded by the cha-1 and unc-17 genes, respectively. Thes...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.173765
更新日期：2015-03-01 00:00:00
abstract：:The larval body wall muscles of Drosophila melanogaster arise by fusion of founder myoblasts (FMs) and fusion-competent myoblasts (FCMs). Sticks-and-Stones (SNS) is expressed on the surface of all FCMs and mediates adhesion with FMs and developing syncytia. Intracellular components essential for myoblast fusion are th...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.083808
更新日期：2008-03-01 00:00:00
abstract：:Although the role of U1 small nuclear RNAs (snRNAs) in 5' splice site recognition is well established, suppressor U1 snRNAs active in intact multicellular animals have been lacking. Here we describe suppression of a 5' splice site mutation in the Drosophila melanogaster white gene (wDR18) by compensatory changes in U1...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Interactions with bacteria play a major role in immune responses, ecology, and evolution of all animals, but they have been neglected until recently in the case of C. elegans. We report a genetic investigation of the interaction of C. elegans with the nematode-specific pathogen Microbacterium nematophilum, which colon...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.045716
更新日期：2005-11-01 00:00:00
abstract：:DNA methylation is a chromatin modification that contributes to epigenetic regulation of gene expression. The inheritance patterns and trans-generational stability of 962 differentially methylated regions (DMRs) were assessed in a panel of 71 near-isogenic lines (NILs) derived from maize (Zea mays) inbred lines B73 an...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.158980
更新日期：2014-03-01 00:00:00
abstract：:Divergence of the hyperthermophilic Archaea, Pyrococcus furiosus and Pyrococcus horikoshii, was assessed by analysis of complete genomic sequences of both species. The average nucleotide identity between the genomic sequences is 70-75% within ORFs. The P. furiosus genome (1.908 mbp) is 170 kbp larger than the P. horik...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:The Spt-Ada-Gcn5-acetyltransferase (SAGA) complex of Saccharomyces cerevisiae is a multifunctional coactivator complex that has been shown to regulate transcription by distinct mechanisms. Previous results have shown that the Spt3 and Spt8 components of SAGA regulate initiation of transcription of particular genes by ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.081976
更新日期：2007-12-01 00:00:00
abstract：:Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects; however, the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes and pathways importa...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.300527
更新日期：2018-02-01 00:00:00
abstract：:Neural ganglia of wild type third-instar larvae of Drosophila melanogaster were incubated for 13 hours at various concentrations of BUdR (1, 3, 9, 27 micrograms/ml). Metaphases were collected with colchicine, stained with Hoechst 33258, and scored under a fluorescence microscope. Metaphases in which the sister chromat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
abstract：:A female frog heterozygous at two unlinked loci, specifying electrophoretic forms of mannosephosphate isomerase (MPI) and malate dehydrogenase (MDH) was crossed to male frogs homozygous for different alleles at each locus. In the offspring approximately ten percent proved to be triploid according to nucleolar and chro...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:Simple meiotic gene conversion tracts produced in wild-type females were compared with those from two meiotic mutants, mei-9 and mei-218. The positions and lengths of conversion tracts were determined by denaturing gradient gels and DNA sequencing. Conversion tracts in wild type averaged 885 base pairs in length, were...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The LmR1 locus, which controls seedling resistance to the blackleg fungus Leptosphaeria maculans in the Brassica napus cultivar Shiralee, was positioned on linkage group N7. Fine genetic mapping in a population of 2500 backcross lines identified three molecular markers that cosegregated with LmR1. Additional linkage m...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.049098
更新日期：2005-12-01 00:00:00
abstract：:The St. Thomas Hospital (STH) rabbit has been previously shown to have a Mendelian form of hypertriglyceridemia, accompanied by accelerated atherosclerosis, and these animals may serve as a useful model for human dyslipoproteinemia syndromes. Here we describe the establishment of a new colony of these STH animals, and...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Heteroduplexes with single base pair mismatches of known sequence were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. A series of transition (G:T and A:C) and transversion (G:A and C:T) mismatches located throughout most of the bacteriophage lambda cI gene h...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:Genetic study of the reproductive barriers between related species plays an essential role in understanding the process of speciation. We developed a new method for mapping all possible factors causing deviations from expected Mendelian segregation ratios in F(2) progeny, which substantially contribute to reproductive...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:Cryptococcus neoformans is a fungal pathogen that causes meningitis in immunocompromised hosts. The organism has a known sexual cycle, and strains of the MATalpha mating type are more virulent than isogenic MATa strains in mice, and they are more common in the environment and infected hosts. A C. neoformans homolog of...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Sir2 is a highly conserved NAD+-dependent histone deacetylase that functions in heterochromatin formation and promotes replicative life span (RLS) in the budding yeast, Saccharomyces cerevisiae Within the yeast rDNA locus, Sir2 is required for efficient cohesin recruitment and maintaining the stability of the tandem a...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302047
更新日期：2019-05-01 00:00:00
abstract：:Various measures have been proposed for characterizing the statistical association that arises between alleles at different loci. Hedrick has compared these measures with the standardized measure D' proposed by Lewontin on the grounds that this latter measure is independent of allele frequency. Although D' has the sam...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:The eukaryotic cytoplasm contains a variety of ribonucleoprotein (RNP) granules in addition to the better-understood membrane-bound organelles. These granules form in response to specific stress conditions and contain a number of signaling molecules important for the control of cell growth and survival. However, relat...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.187419
更新日期：2016-07-01 00:00:00
abstract：:Genetic control over chromosome stability in the interspecific hybrid embryos of Hordeum vulgare and H. bulbosum has been hypothesized to reside on specific chromosomes. In this study, crosses between the primary trisomic lines for the seven different H. vulgare chromosomes and tetraploid H. bulbosum revealed that bot...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
abstract：:To investigate the genetic basis of maize adaptation to temperate climate, collections of 375 inbred lines and 275 landraces, representative of American and European diversity, were evaluated for flowering time under short- and long-day conditions. The inbred line collection was genotyped for 55 genomewide simple sequ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.048603
更新日期：2006-04-01 00:00:00
abstract：:The mutational origin and subsequent evolution of de novo genes, which are hypothesized to be genes of recent origin that are not obviously related to ancestral coding sequence, are poorly understood. However, accumulating evidence suggests that such genes may often function in male reproduction. Here we use testis-de...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.069245
更新日期：2007-06-01 00:00:00
abstract：:Birds are characterized by female heterogamety; females carry the Z and W sex chromosomes, while males have two copies of the Z chromosome. We suggest here that full differentiation of the Z and W sex chromosomes of birds did not take place until after the split of major contemporary lineages, in the late Cretaceous. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:A Plasmodium-refractory strain of Anopheles gambiae melanotically encapsulates many species of Plasmodium, whereas wild-type mosquitoes are usually susceptible. This encapsulation trait can also be observed by studying the response of refractory and susceptible strains to intrathoracically injected CM-Sephadex beads. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
