Marker-based cloning of the region containing the UhAvr1 avirulence gene from the basidiomycete barley pathogen Ustilago hordei.

Abstract:

:Race-cultivar specialization during the interaction of the basidiomycete smut pathogen Ustilago hordei with its barley host was described in the 1940s. Subsequent genetic analyses revealed the presence of dominant avirulence genes in the pathogen that conform to the gene-for-gene theory. This pathosystem therefore presents an opportunity for the molecular genetic characterization of fungal genes controlling avirulence. We performed a cross between U. hordei strains to obtain 54 progeny segregating for three dominant avirulence genes on three differential barley cultivars. Bulked segregant analysis was used to identify RAPD and AFLP markers tightly linked to the avirulence gene UhAvr1. The UhAvr1 gene is located in an area containing repetitive DNA and this region is undetectable in cosmid libraries prepared from the avirulent parental strain. PCR and hybridization probes developed from the linked markers were therefore used to identify cosmid clones from the virulent (Uhavr1) parent. By walking on Uhavr1-linked cosmid clones, a nonrepetitive, nearby probe was found that recognized five overlapping BAC clones spanning 170 kb from the UhAvr1 parent. A contig of the clones in the UhAvr1 region was constructed and selected probes were used for RFLP analysis of the segregating population. This approach genetically defined an approximately 80-kb region that carries the UhAvr1 gene and provided cloned sequences for subsequent genetic analysis. UhAvr1 represents the first avirulence gene cloned from a basidiomycete plant pathogen.

journal_name

Genetics

journal_title

Genetics

authors

Linning R,Lin D,Lee N,Abdennadher M,Gaudet D,Thomas P,Mills D,Kronstad JW,Bakkeren G

doi

10.1534/genetics.166.1.99

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

99-111

issue

1

eissn

0016-6731

issn

1943-2631

pii

166/1/99

journal_volume

166

pub_type

杂志文章

相关文献

GENETICS文献大全
  • Recovery of heritable, transposon-induced, mutant alleles of the rf 2 nuclear restorer of T-cytoplasm maize.

    abstract::T (Texas) cytoplasm is associated with a mitochondrial disruption that is phenotypically expressed during microsporogenesis resulting in male sterility. Restoration of pollen fertility in T-cytoplasm maize is controlled by dominant alleles at two unlinked, complementary, nuclear-encoded genes, rf1 and rf2. As a first ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Schnable PS,Wise RP

    更新日期:1994-03-01 00:00:00

  • Architectural and functional diversity of polycomb group response elements in Drosophila.

    abstract::Polycomb group response elements (PREs) play an essential role in gene regulation by the Polycomb group (PcG) repressor proteins in Drosophila. PREs are required for the recruitment and maintenance of repression by the PcG proteins. PREs are made up of binding sites for multiple DNA-binding proteins, but it is still u...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.113.153247

    authors: Brown JL,Kassis JA

    更新日期:2013-10-01 00:00:00

  • Efficiency of the use of pedigree and molecular marker information in conservation programs.

    abstract::The value of molecular markers and pedigree records, separately or in combination, to assist in the management of conserved populations has been tested. The general strategy for managing the population was to optimize contributions of parents to the next generation for minimizing the global weighted coancestry. Strate...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.037325

    authors: Fernández J,Villanueva B,Pong-Wong R,Toro MA

    更新日期:2005-07-01 00:00:00

  • Biased short tract repair of palindromic loop mismatches in mammalian cells.

    abstract::Mismatch repair of palindromic loops in the presence or absence of single-base mismatches was investigated in wild-type and mismatch-binding defective mutant Chinese hamster ovary cells. Recombination intermediates with a maximum heteroduplex DNA (hDNA) region of 697 bp contained a centrally located, phenotypically si...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Taghian DG,Hough H,Nickoloff JA

    更新日期:1998-03-01 00:00:00

  • An investigation of heterochromatin domains on the fourth chromosome of Drosophila melanogaster.

    abstract::The banded portion of Drosophila melanogaster chromosome 4 exhibits euchromatic and heterochromatic characteristics. Reminiscent of heterochromatin, it contains a high percentage of repetitive elements, does not undergo recombination, and exhibits high levels of HP1 and histone-3 lysine-9 dimethylation. However, in th...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.107.081828

    authors: Riddle NC,Leung W,Haynes KA,Granok H,Wuller J,Elgin SC

    更新日期:2008-03-01 00:00:00

  • Host and phi X 174 mutations affecting the morphogenesis or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediate.

    abstract::The morphogenetic pathway of bacteriophage phi X 174 was investigated in rep mutant hosts that specifically block stage III single-stranded DNA synthesis. The defects conferred by the mutant rep protein most likely affect the formation or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediat...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Ekechukwu MC,Oberste DJ,Fane BA

    更新日期:1995-08-01 00:00:00

  • The let-60 locus controls the switch between vulval and nonvulval cell fates in Caenorhabditis elegans.

    abstract::During induction of the Caenorhabditis elegans hermaphrodite vulva by the anchor cell of the gonad, six multipotent vulval precursor cells (VPCs) have two distinct fates: three VPCs generate the vulva and the other three VPCs generate nonspecialized hypodermis. Genes that control the fates of the VPCs in response to t...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Han M,Aroian RV,Sternberg PW

    更新日期:1990-12-01 00:00:00

  • Mapping quantitative trait loci for milk production and health of dairy cattle in a large outbred pedigree.

    abstract::Quantitative trait loci (QTL) affecting milk production and health of dairy cattle were mapped in a very large Holstein granddaughter design. The analysis included 1794 sons of 14 sires and 206 genetic markers distributed across all 29 autosomes and flanking an estimated 2497 autosomal cM using Kosambi's mapping funct...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Zhang Q,Boichard D,Hoeschele I,Ernst C,Eggen A,Murkve B,Pfister-Genskow M,Witte LA,Grignola FE,Uimari P,Thaller G,Bishop MD

    更新日期:1998-08-01 00:00:00

  • OrthoList 2: A New Comparative Genomic Analysis of Human and Caenorhabditis elegans Genes.

    abstract::OrthoList, a compendium of Caenorhabditis elegans genes with human orthologs compiled in 2011 by a meta-analysis of four orthology-prediction methods, has been a popular tool for identifying conserved genes for research into biological and disease mechanisms. However, the efficacy of orthology prediction depends on th...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.118.301307

    authors: Kim W,Underwood RS,Greenwald I,Shaye DD

    更新日期:2018-10-01 00:00:00

  • Proteasome mutants, pre4-2 and ump1-2, suppress the essential function but not the mitochondrial RNase P function of the Saccharomyces cerevisiae gene RPM2.

    abstract::The Saccharomyces cerevisiae nuclear gene RPM2 encodes a component of the mitochondrial tRNA-processing enzyme RNase P. Cells grown on fermentable carbon sources do not require mitochondrial tRNA processing activity, but still require RPM2, indicating an additional function for the Rpm2 protein. RPM2-null cells arrest...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Lutz MS,Ellis SR,Martin NC

    更新日期:2000-03-01 00:00:00

  • Synaptonemal complex and recombination nodules in wild-type Drosophila melanogaster females.

    abstract::Electron microscope serial section reconstruction analysis of all zygotene-pachytene nuclei of meiotic cells from three wild-type germaria (a subunit of the ovary containing the early meiotic stages arrayed in temporal developmental sequence) of Drosophila melanogaster females corroborates and extends earlier observat...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Carpenter AT

    更新日期:1979-06-01 00:00:00

  • Multiple Transcriptional and Post-transcriptional Pathways Collaborate to Control Sense and Antisense RNAs of Tf2 Retroelements in Fission Yeast.

    abstract::Retrotransposons are mobile genetic elements that colonize eukaryotic genomes by replicating through an RNA intermediate. As retrotransposons can move within the host genome, defense mechanisms have evolved to repress their potential mutagenic activities. In the fission yeast Schizosaccharomyces pombe, the mRNA of Tf2...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.116.193870

    authors: Mallet PL,Larochelle M,Bachand F

    更新日期:2017-02-01 00:00:00

  • Drosophila calmodulin mutants with specific defects in the musculature or in the nervous system.

    abstract::We have studied lethal mutations in the single calmodulin gene (Cam) of Drosophila to gain insight into the in vivo functions of this important calcium sensor. As a result of maternal calmodulin (CaM) in the mature egg, lethality is delayed until the postembryonic stages. Prior to death in the first larval instar, Cam...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Wang B,Sullivan KM,Beckingham K

    更新日期:2003-11-01 00:00:00

  • Mapping quantitative trait loci for anxiety in chromosome substitution strains of mice.

    abstract::Anxious behavior in the mouse is a complex quantitative phenotype that varies widely among inbred mouse strains. We examined a panel of chromosome substitution strains bearing individual A/J chromosomes in an otherwise C57BL/6J background in open-field and light-dark transition tests. Our results confirmed previous re...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.031492

    authors: Singer JB,Hill AE,Nadeau JH,Lander ES

    更新日期:2005-02-01 00:00:00

  • Genetic dissection of clonally inherited genomes of Poeciliopsis: II. Investigation of a silent carboxylesterase allele.

    abstract::According to the ratchet mechanism hypothesis, deleterious mutations should accumulate in clonal genomes of unisexual fish of the genus Poeciliopsis. This study defines one such mutant, a silent carboxylesterase allele (ES-5 degrees) which is found in the heterozygous condition in a particular population of P. monacha...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Spinella DG,Vrijenhoek RC

    更新日期:1982-02-01 00:00:00

  • Spatial autocorrelation of genotypes under directional selection.

    abstract::The spatial distributions of genetic variation under selection-mutation equilibrium within populations that have limited dispersal are investigated. The results show that directional selection with moderate strength rapidly reduces the amount of genetic structure and spatial autocorrelations far below that predicted f...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Epperson BK

    更新日期:1990-03-01 00:00:00

  • Virility Deficiency and the Sex-Ratio Trait in DROSOPHILA PSEUDOOBSCURA. I. Sperm Displacement and Sexual Selection.

    abstract::The Sex-Ratio (SR) phenomenon is associated with the X chromosome of many species of Drosophila. Males carrying SR transmit predominantly sperm bearing the X chromosome. SR, therefore, has a very significant advantage in segregation. This paper provides an experimental analysis of the role of virility selection in pre...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Wu CI

    更新日期:1983-11-01 00:00:00

  • Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata).

    abstract::The complete nucleotide sequence of the 14,771-bp-long mitochondrial (mt) DNA of a urochordate (Chordata)-the ascidian Halocynthia roretzi-was determined. All the Halocynthia mt-genes were found to be located on a single strand, which is rich in T and G rather than in A and C. Like nematode and Mytilus edulis mtDNAs, ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Yokobori Si,Ueda T,Feldmaier-Fuchs G,Pääbo S,Ueshima R,Kondow A,Nishikawa K,Watanabe K

    更新日期:1999-12-01 00:00:00

  • Spatial structure of two-locus genotypes under isolation by distance.

    abstract::Extensive Monte Carlo simulations are conducted of spatial distributions of two-locus genotypes in large, continuous populations under isolation by distance models. The results show that substantial patches of double homozygotes are present in the spatial structures, even when loci are unlinked. The stochastic spread ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Epperson BK

    更新日期:1995-05-01 00:00:00

  • Molecular-genetic characterization of CMS-S restorer-of-fertility alleles identified in Mexican maize and teosinte.

    abstract::Restorer-of-fertility (Rf) alleles for S-type cytoplasmic male sterility (CMS-S) are prevalent in Mexican races of maize and teosinte. Forty-five Rf alleles from 26 races of maize and 6 Rf alleles from different accessions of teosinte were found to be homozygous viable, consistent with the hypothesis that they are nat...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.166.2.959

    authors: Gabay-Laughnan S,Chase CD,Ortega VM,Zhao L

    更新日期:2004-02-01 00:00:00

  • Impact of amplified fragment length polymorphism size homoplasy on the estimation of population genetic diversity and the detection of selective loci.

    abstract::AFLP markers are becoming one of the most popular tools for genetic analysis in the fields of evolutionary genetics and ecology and conservation of genetic resources. The technique combines a high-information content and fidelity with the possibility of carrying out genomewide scans. However, a potential problem with ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.107.083246

    authors: Caballero A,Quesada H,Rolán-Alvarez E

    更新日期:2008-05-01 00:00:00

  • Conidial Morphogenesis and Septin-Mediated Plant Infection Require Smo1, a Ras GTPase-Activating Protein in Magnaporthe oryzae.

    abstract::The pathogenic life cycle of the rice blast fungus Magnaporthe oryzae involves a series of morphogenetic changes, essential for its ability to cause disease. The smo mutation was identified > 25 years ago, and affects the shape and development of diverse cell types in M. oryzae, including conidia, appressoria, and asc...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.118.301490

    authors: Kershaw MJ,Basiewicz M,Soanes DM,Yan X,Ryder LS,Csukai M,Oses-Ruiz M,Valent B,Talbot NJ

    更新日期:2019-01-01 00:00:00

  • Genetic analysis of metabolic crosstalk and its impact on thiamine synthesis in Salmonella typhimurium.

    abstract::The first five steps in de novo purine biosynthesis are involved in the formation of the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine. We show here that the first enzyme in de novo purine biosynthesis, PurF, is required for thiamine synthesis during aerobic growth on some but not other carbon s...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Petersen L,Enos-Berlage J,Downs DM

    更新日期:1996-05-01 00:00:00

  • Chromosome-scale genetic mapping using a set of 16 conditionally stable Saccharomyces cerevisiae chromosomes.

    abstract::We have created a resource to rapidly map genetic traits to specific chromosomes in yeast. This mapping is done using a set of 16 yeast strains each containing a different chromosome with a conditionally functional centromere. Conditional centromere function is achieved by integration of a GAL1 promoter in cis to cent...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.108.087999

    authors: Reid RJ,Sunjevaric I,Voth WP,Ciccone S,Du W,Olsen AE,Stillman DJ,Rothstein R

    更新日期:2008-12-01 00:00:00

  • Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM protein.

    abstract::The cdc19+ gene encodes an essential member of the MCM family of replication proteins in Schizosaccharomyces pombe. We have examined the structure and function of the Cdc19p protein using molecular and genetic approaches. We find that overproduction of wild-type Cdc19p in wild-type cells has no effect, but cdc19-P1 mu...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Forsburg SL,Sherman DA,Ottilie S,Yasuda JR,Hodson JA

    更新日期:1997-11-01 00:00:00

  • Novel acid phosphatase in Candida glabrata suggests selective pressure and niche specialization in the phosphate signal transduction pathway.

    abstract::Evolution through natural selection suggests unnecessary genes are lost. We observed that the yeast Candida glabrata lost the gene encoding a phosphate-repressible acid phosphatase (PHO5) present in many yeasts including Saccharomyces cerevisiae. However, C. glabrata still had phosphate starvation-inducible phosphatas...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.110.120824

    authors: Orkwis BR,Davies DL,Kerwin CL,Sanglard D,Wykoff DD

    更新日期:2010-11-01 00:00:00

  • A transgene-induced mitotic arrest mutation in the mouse allelic with Oligosyndactylism.

    abstract::Oligosyndactylism (Os) is a radiation-induced mutation on mouse chromosome 8 associated with early postimplantation lethality in homozygotes and abnormal development of the limbs and kidneys in heterozygotes. The recessive lethal effect of Os is due to a mitotic block of the embryonic cells that becomes apparent at th...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Pravtcheva DD,Wise TL

    更新日期:1996-12-01 00:00:00

  • The three-dimensional organization of polytene nuclei in male Drosophila melanogaster with compound XY or ring X chromosomes.

    abstract::The three-dimensional organization of polytene chromosomes within nuclei containing rearranged X chromosomes was examined in male Drosophila melanogaster. Salivary glands of third instar larvae containing either an inverted X chromosome (YSX.YL, In(1)EN/O) or a ring X chromosome (R(1) 2/BSYy+) were fixed, embedded, an...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Mathog D,Sedat JW

    更新日期:1989-02-01 00:00:00

  • Analysis of Nondisjunction Induced by the R-X1 Deficiency during Microsporogenesis in Zea Mays L.

    abstract::The r-X1 deficiency in maize induces nondisjunction at the second mitotic division during embryo sac formation. However, it was not known if this deficiency also induces nondisjunction during the microspore divisions. Microsporogenesis in plants lacking or containing this deficiency was compared using two approaches. ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Zhao ZY,Weber DF

    更新日期:1988-08-01 00:00:00

  • A Polycomb and GAGA dependent silencer adjoins the Fab-7 boundary in the Drosophila bithorax complex.

    abstract::The homeotic genes of the Drosophila bithorax complex are controlled by a large cis-regulatory region that ensures their segmentally restricted pattern of expression. A deletion that removes the Frontabdominal-7 cis-regulatory region (Fab-7') dominantly transforms parasegment 11 into parasegment 12. Previous studies s...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Hagstrom K,Muller M,Schedl P

    更新日期:1997-08-01 00:00:00