Transmission dynamics of heritable silencing induced by double-stranded RNA in Caenorhabditis elegans.

Abstract:

:Heritable silencing effects are gene suppression phenomena that can persist for generations after induction. In the majority of RNAi experiments conducted in Caenorhabditis elegans, the silencing response results in a hypomorphic phenotype where the effects recede after the F1 generation. F2 and subsequent generations revert to the original phenotype. Specific examples of transgenerational RNAi in which effects persist to the F2 generation and beyond have been described. In this study, we describe a systematic pedigree-based analysis of heritable silencing processes resulting from initiation of interference targeted at the C. elegans oocyte maturation factor oma-1. Heritable silencing of oma-1 is a dose-dependent process where the inheritance of the silencing factor is unequally distributed among the population. Heritability is not constant over generational time, with silenced populations appearing to undergo a bottleneck three to four generations following microinjection of RNA. Transmission of silencing through these generations can be through either maternal or paternal gamete lines and is surprisingly more effective through the male gametic line. Genetic linkage tests reveal that silencing in the early generations is transmitted independently of the original targeted locus, in a manner indicative of a diffusible epigenetic element.

journal_name

Genetics

journal_title

Genetics

authors

Alcazar RM,Lin R,Fire AZ

doi

10.1534/genetics.108.089433

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

1275-88

issue

3

eissn

0016-6731

issn

1943-2631

pii

genetics.108.089433

journal_volume

180

pub_type

杂志文章

相关文献

GENETICS文献大全
  • Deciphering Sex-Specific Genetic Architectures Using Local Bayesian Regressions.

    abstract::Many complex human traits exhibit differences between sexes. While numerous factors likely contribute to this phenomenon, growing evidence from genome-wide studies suggest a partial explanation: that males and females from the same population possess differing genetic architectures. Despite this, mapping gene-by-sex (...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.120.303120

    authors: Funkhouser SA,Vazquez AI,Steibel JP,Ernst CW,Los Campos G

    更新日期:2020-05-01 00:00:00

  • Bromodeoxyuridine-induced mutations in synchronous Chinese hamster cells: temporal induction of 6-thioguanine and ouabain resistance during DNA replication.

    abstract::Mutations were induced in synchronous Chinese hamster cells by bromodeoxyuridine (BUdR) incorporated into cells for one-hour periods in the cell cycle. There was a very pronounced temporal dependence during the first half of the DNA synthesis period for the induction of damage leading to 6-thioguanine (6TG) and ouabai...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Burki HJ,Aebersold PM

    更新日期:1978-10-01 00:00:00

  • High genetic diversity in the chemoreceptor superfamily of Caenorhabditis elegans.

    abstract::We investigated genetic polymorphism in the Caenorhabditis elegans srh and str chemoreceptor gene families, each of which consists of approximately 300 genes encoding seven-pass G-protein-coupled receptors. Almost one-third of the genes in each family are annotated as pseudogenes because of apparent functional defects...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.035329

    authors: Stewart MK,Clark NL,Merrihew G,Galloway EM,Thomas JH

    更新日期:2005-04-01 00:00:00

  • Chromosomal patterns of microsatellite variability contrast sharply in African and non-African populations of Drosophila melanogaster.

    abstract::Levels of neutral variation are influenced by background selection and hitchhiking. The relative contribution of these evolutionary forces to the distribution of neutral variation is still the subject of ongoing debates. Using 133 microsatellites, we determined levels of variability on X chromosomes and autosomes in A...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Kauer M,Zangerl B,Dieringer D,Schlötterer C

    更新日期:2002-01-01 00:00:00

  • Measuring selection coefficients affecting the alcohol dehydrogenase polymorphism in Drosophila melanogaster.

    abstract::This paper describes a perturbation experiment on the frequency of the F and S Alcohol dehydrogenase (Adh) alleles of D. melanogaster. Fifty-four iso-female lines set up from three wild populations and with initial F frequencies of either 0.25, 0.50 or 0.75 were maintained on standard laboratory food medium at 22 degr...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Wilson SR,Oakeshott JG,Gibson JB,Anderson PR

    更新日期:1982-01-01 00:00:00

  • Control of Locomotory Behavior of Caenorhabditis elegans by the Immunoglobulin Superfamily Protein RIG-3.

    abstract::Cell surface immunoglobulin superfamily (IgSF) proteins play important roles in the development and function of the nervous system . Here we define the role of a Caenorhabditis elegans IgSF protein, RIG-3, in the function of the AVA command interneuron. This study reveals that RIG-3 regulates the abundance of the glut...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.119.302872

    authors: Bhardwaj A,Pandey P,Babu K

    更新日期:2020-01-01 00:00:00

  • The Inheritance of Enzyme Variants for Tyrosine Aminotransferase, Nadp-Dependent Malate Dehydrogenase, Nadp-Dependent Isocitrate Dehydrogenase, and Tetrazolium Oxidase in TETRAHYMENA PYRIFORMIS, Syngen 1.

    abstract::The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzym...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Borden D,Miller ET,Nanney DL,Whitt GS

    更新日期:1973-08-01 00:00:00

  • Recent and massive expansion of the mating-type-specific region in the smut fungus Microbotryum.

    abstract::The presence of large genomic regions with suppressed recombination (SR) is a key shared property of some sex- and mating-type determining (mat) chromosomes identified to date in animals, plants, and fungi. Why such regions form and how they evolve remain central questions in evolutionary genetics. The smut fungus Mic...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.114.171702

    authors: Whittle CA,Votintseva A,Ridout K,Filatov DA

    更新日期:2015-03-01 00:00:00

  • Dominance genetic variance for traits under directional selection in Drosophila serrata.

    abstract::In contrast to our growing understanding of patterns of additive genetic variance in single- and multi-trait combinations, the relative contribution of nonadditive genetic variance, particularly dominance variance, to multivariate phenotypes is largely unknown. While mechanisms for the evolution of dominance genetic v...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.115.175489

    authors: Sztepanacz JL,Blows MW

    更新日期:2015-05-01 00:00:00

  • Inversions fail to account for allozyme clines.

    abstract::Allozyme and inversion data from natural populations of Drosophila melanogaster from the eastern United States were analyzed to determine whether the clines at allozyme loci are due to nonrandom associations with common cosmopolitan inversions. All inversions show strong clines. Clines were large and significant for h...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Voelker RA,Cockerham CC,Johnson FM,Schaffer HE,Mukai T,Mettler LE

    更新日期:1978-03-01 00:00:00

  • Quantitative Measurement of the Ability of Different Mutagens to Induce an Inherited Change in Phenotype to Allow Maltose Utilization in Suspension Cultures of the Soybean, GLYCINE MAX (L.) Merr.

    abstract::USING A NEWLY DEVELOPED PLATING SYSTEM, WE HAVE MEASURED CELL SURVIVAL AND THE FREQUENCIES OF VARIATION IN AN INHERITED TRAIT AFTER TREATMENT OF SOYBEAN CELL SUSPENSIONS WITH DIFFERENT MUTAGENS: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-Methyl-N'-nitro-N-nitroso-guanidine (MNNG), hycanthone (1-{[2...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Weber G,Lark KG

    更新日期:1980-09-01 00:00:00

  • Allozyme Segregation Ratios in the Interspecific Cross CUCURBITA MAXIMAxC. ECUADORENSIS Suggest That Hybrid Breakdown Is Not Caused by Minor Alterations in Chromosome Structure.

    abstract::The parentals of the interspecific cross Cucurbita maxima xC. ecuadorensis had different isozyme phenotypes for 12 enzyme systems. Characterization of the systems demonstrated that the expression and intracellular distribution of the isozymes were similar to those in other plant taxa; however, a considerable number of...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Weeden NF,Robinson RW

    更新日期:1986-10-01 00:00:00

  • Evolutionary Rescue over a Fitness Landscape.

    abstract::Evolutionary rescue describes a situation where adaptive evolution prevents the extinction of a population facing a stressing environment. Models of evolutionary rescue could in principle be used to predict the level of stress beyond which extinction becomes likely for species of conservation concern, or, conversely, ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.118.300908

    authors: Anciaux Y,Chevin LM,Ronce O,Martin G

    更新日期:2018-05-01 00:00:00

  • A screen for mutations that suppress the phenotype of Drosophila armadillo, the beta-catenin homolog.

    abstract::During development signaling pathways coordinate cell fates and regulate the choice between cell survival or programmed cell death. The well-conserved Wingless/Wnt pathway is required for many developmental decisions in all animals. One transducer of the Wingless/Wnt signal is Armadillo/beta-catenin. Drosophila Armadi...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Cox RT,McEwen DG,Myster DL,Duronio RJ,Loureiro J,Peifer M

    更新日期:2000-08-01 00:00:00

  • Genetic and physical analysis of the M26 recombination hotspot of Schizosaccharomyces pombe.

    abstract::The ade6-M26 mutation of Schizosaccharomyces pombe has previously been reported to stimulate ade6 intragenic meiotic recombination. We report here that the ade6-M26 mutation is a single G----T nucleotide change, that M26 stimulated recombination within ade6 but not at other distinct loci, and that M26 stimulated meiot...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Ponticelli AS,Sena EP,Smith GR

    更新日期:1988-07-01 00:00:00

  • Gene expression analysis of the function of the male-specific lethal complex in Drosophila.

    abstract::Dosage compensation refers to the equal expression of X-linked genes despite the difference in copy number between the two sexes. The male-specific lethal (MSL) complex is concentrated on the X chromosome in males. A gene expression assay for embryos was developed to examine the function of this complex. In mutant mal...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.104.036020

    authors: Bhadra MP,Bhadra U,Kundu J,Birchler JA

    更新日期:2005-04-01 00:00:00

  • Genetic control of malate dehydrogenase isozymes in maize.

    abstract::At least six nuclear loci are responsible for the genetic control of malate dehydrogenase (L-malate: NAD oxidoreductase; EC 1.1.1.37; MDH) in coleoptiles of maize. Three independently segregating loci (Mdh1, Mdh2, Mdh3) govern the production of MDH isozymes resistant to inactivation by ascorbic acid and found largely ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Goodman MM,Stuber CW,Lee CN,Johnson FM

    更新日期:1980-01-01 00:00:00

  • Multiple mechanisms for degradation of bacteriophage T4 soc mRNA.

    abstract::The dmd gene of bacteriophage T4 is required for regulation of mRNA stability in a stage-dependent manner during infection. When this gene is mutated, late genes are globally silenced because of rapid degradation of mRNAs. To investigate the mechanism of such mRNA degradation, we analyzed the late gene soc transcripts...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Kai T,Yonesaki T

    更新日期:2002-01-01 00:00:00

  • A genome-scan method to identify selected loci appropriate for both dominant and codominant markers: a Bayesian perspective.

    abstract::Identifying loci under natural selection from genomic surveys is of great interest in different research areas. Commonly used methods to separate neutral effects from adaptive effects are based on locus-specific population differentiation coefficients to identify outliers. Here we extend such an approach to estimate d...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.108.092221

    authors: Foll M,Gaggiotti O

    更新日期:2008-10-01 00:00:00

  • The genetic structure of natural populations of Drosophila melanogaster. XII. Linkage disequilibrium in a large local population.

    abstract::Seven hundred and three second chromosomes were extracted from a Raleigh, North Carolina population of Drosophila melanogaster in 1970. Additionally, four hundred and eighty-nine third chromosomes were extracted from a large cage population founded from the flies in the 1970 Raleigh collection. The alpha glycerol-3-ph...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Muki T,Watanabe TK,Yamaguchi O

    更新日期:1974-08-01 00:00:00

  • A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae.

    abstract::Loss of heterozygosity (LOH) can be a driving force in the evolution of mitotic/somatic diploid cells, and cellular changes that increase the rate of LOH have been proposed to facilitate this process. In the yeast Saccharomyces cerevisiae, spontaneous LOH occurs by a number of mechanisms including chromosome loss and ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.108.089250

    authors: Andersen MP,Nelson ZW,Hetrick ED,Gottschling DE

    更新日期:2008-07-01 00:00:00

  • Reversion from suppression to nonsuppression in SUQ5 [psi+] strains of yeast: the classificaion of mutations.

    abstract::Reversion from the suppressed to nonsuppressed phenotype in strains of geno;type SUQ5 [psi+] ade2-1 his5-2 lys1-1 can1-100 ura3-1 has been induced by treatment with ethyl methanesulphonate, nitrosoguanidine or UV (254 nm) light. Spontaneously occurring revertants have also been selected by two different methods. Rever...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Cox BS,Tuite MF,Mundy CJ

    更新日期:1980-07-01 00:00:00

  • On the identification of the rosy locus DNA in Drosophila melanogaster: intragenic recombination mapping of mutations associated with insertions and deletions.

    abstract::DNA extracts of several rosy-mutation-bearing strains were associated with large insertions and deletions in a defined region of the molecular map believed to include the rosy locus DNA. Large-scale, intragenic mapping experiments were carried out that localized these mutations within the boundaries of the previously ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Clark SH,McCarron M,Love C,Chovnick A

    更新日期:1986-04-01 00:00:00

  • Terminal synthesis of xanthommatin in Drosophila melanogaster. 3. Mutational pleiotropy and pigment granule association of phenoxazinone synthetase.

    abstract::Phenoxazinone synthetase, which catalyzes the condensation of 3-hydroxykynurenine to xanthommatin, the brown eye pigment of Drosophila, is shown to exist in association with a particle which resembles the cytologically defined Type I pigment granule. Several classical eye color mutants (v, cn, st, ltd, cd, w), includi...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Phillips JP,Forrest HS,Kulkarni AD

    更新日期:1973-01-01 00:00:00

  • Structure, expression and phylogenetic analysis of the gene encoding actin I in Pneumocystis carinii.

    abstract::Actin is a major component of the cytoskeleton and one of the most abundant proteins found in eukaryotic cells. Comparative sequence analysis shows that this essential gene has been highly conserved throughout eukaryotic evolution making it useful for phylogenetic analysis. Complete cDNA clones for the actin-encoding ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Fletcher LD,McDowell JM,Tidwell RR,Meagher RB,Dykstra CC

    更新日期:1994-07-01 00:00:00

  • Respiratory chain complex I is essential for sexual development in neurospora and binding of iron sulfur clusters are required for enzyme assembly.

    abstract::We have cloned and disrupted in vivo, by repeat-induced point mutations, the nuclear gene coding for an iron sulfur subunit of complex I from Neurospora crassa, homologue of the mammalian TYKY protein. Analysis of the obtained mutant nuo21.3c revealed that complex I fails to assemble. The peripheral arm of the enzyme ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Duarte M,Videira A

    更新日期:2000-10-01 00:00:00

  • Recombination in relation to ultraviolet sensitivity in Chlamydomonas reinhardi.

    abstract::A mutant strain of Chlamydomonas reinhardi which is UV sensitive as a result of a single-gene chromosomal mutation has also been found to exhibit reduced recombination. In crosses homozygous for the mutant allele, a reduction in recombination frequency was demonstrated in two different linkage groups and in three diff...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Rosen H,Ebersold WT

    更新日期:1972-06-01 00:00:00

  • A perspective on micro-evo-devo: progress and potential.

    abstract::The term "micro-evo-devo" refers to the combined study of the genetic and developmental bases of natural variation in populations and the evolutionary forces that have shaped this variation. It thus represents a synthesis of the fields of evolutionary developmental biology and population genetics. As has been pointed ...

    journal_title:Genetics

    pub_type: 杂志文章,评审

    doi:10.1534/genetics.113.156463

    authors: Nunes MD,Arif S,Schlötterer C,McGregor AP

    更新日期:2013-11-01 00:00:00

  • Sequence-dependent gene conversion: can duplicated genes diverge fast enough to escape conversion?

    abstract::Conversion between duplicated genes limits their independent evolution. Models in which conversion frequencies decrease as genes diverge are examined to determine conditions under which genes can "escape" further conversion and hence escape from a gene family. A review of results from various recombination systems sug...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:

    authors: Walsh JB

    更新日期:1987-11-01 00:00:00

  • HaploBlocker: Creation of Subgroup-Specific Haplotype Blocks and Libraries.

    abstract::The concept of haplotype blocks has been shown to be useful in genetics. Fields of application range from the detection of regions under positive selection to statistical methods that make use of dimension reduction. We propose a novel approach ("HaploBlocker") for defining and inferring haplotype blocks that focuses ...

    journal_title:Genetics

    pub_type: 杂志文章

    doi:10.1534/genetics.119.302283

    authors: Pook T,Schlather M,de Los Campos G,Mayer M,Schoen CC,Simianer H

    更新日期:2019-08-01 00:00:00