Abstract:
:This study concerns the problem of odor receptor gene choice in the fruit fly Drosophila melanogaster. From a family of 60 Odor receptor genes, only one or a small number are selected for expression by each olfactory receptor neuron. Little is known about how an olfactory receptor neuron selects a receptor, or how the nucleotide sequences flanking a receptor gene dictate its expression in a particular neuron. Previous investigation has primarily concerned the maxillary palp, the simpler of the fly's two olfactory organs. Here we focus on genes encoding four antennal receptors that respond to fly odors in an in vivo expression system. To investigate the logic of odor receptor expression, we carry out a genetic analysis of their upstream regulatory sequences. Deletion analysis reveals that relatively short regulatory regions are sufficient to confer expression in the appropriate neurons, with limited if any misexpression. We find evidence for both positive and negative regulation. Multiple repressive functions restrict expression to the antenna, to a region of the antenna, and to neurons. Through deletion and base substitution mutagenesis we identify GCAATTA elements and find evidence that they act in both positive and negative regulation.
journal_name
Geneticsjournal_title
Geneticsauthors
Miller CJ,Carlson JRdoi
10.1534/genetics.110.117622subject
Has Abstractpub_date
2010-09-01 00:00:00pages
79-95issue
1eissn
0016-6731issn
1943-2631pii
genetics.110.117622journal_volume
186pub_type
杂志文章相关文献
GENETICS文献大全abstract::In diploid Saccharomyces cerevisiae cells, bud-site selection is determined by two cortical landmarks, Bud8p and Bud9p, at the distal and proximal poles, respectively. Their localizations depend on the multigenerational proteins Rax1p/Rax2p. Many genes involved in bud-site selection were identified previously by genom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.128231
更新日期:2011-08-01 00:00:00
abstract::Wolbachia-induced cytoplasmic incompatibility (CI) is expressed when infected males are crossed with either uninfected females or females infected with Wolbachia of different CI specificity. In diploid insects, CI results in embryonic mortality, apparently due to the the loss of the paternal set of chromosomes, usuall...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.086488
更新日期:2008-04-01 00:00:00
abstract::Biofilms are microbial communities that form on surfaces. They are the primary form of microbial growth in nature and can have detrimental impacts on human health. Some strains of the budding yeast Saccharomyces cerevisiae form colony biofilms, and there is substantial variation in colony architecture between biofilm-...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.142067
更新日期:2013-02-01 00:00:00
abstract::A genotype probability index (GPI) is proposed to indicate the information content of genotype probabilities derived from a segregation analysis. Typically, some individuals are genotyped at a marker locus or a quantitative trait locus, and segregation analysis is used to make genotype inferences about ungenotyped rel...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Obtaining accurate estimates of the genetic covariance matrix Sigma(G) for multivariate data is a fundamental task in quantitative genetics and important for both evolutionary biologists and plant or animal breeders. Classical methods for estimating Sigma(G) are well known to suffer from substantial sampling errors; i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.113381
更新日期:2010-07-01 00:00:00
abstract::Simple meiotic gene conversion tracts produced in wild-type females were compared with those from two meiotic mutants, mei-9 and mei-218. The positions and lengths of conversion tracts were determined by denaturing gradient gels and DNA sequencing. Conversion tracts in wild type averaged 885 base pairs in length, were...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::We have analyzed two variants of Drosophila melanogaster (RS and RE) which lead to the dual phenotype of elevated DDC activity and increased resistance to dietary alpha-methyldopa relative to Oregon-R controls. Both phenotypes show tight genetic linkage to the dopa decarboxylase, Ddc, and l(2)amd genes (i.e., less tha...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::The genomes of all organisms are subject to continuous bombardment of deleterious genomic mutations (DGM). Our ability to accurately estimate various parameters of DGM has profound significance in population and evolutionary genetics. The Deng-Lynch method can estimate the parameters of DGM in natural selfing and outc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::Several adhesins are induced by pheromones during mating in Saccharomyces cerevisiae, including Aga1p, Aga2p, Sag1p (Agalpha1p), and Fig2p. These four proteins all participate in or influence a well-studied agglutinin interaction mediated by Aga1p-Aga2p complexes and Sag1p; however, they also play redundant and essent...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.100073
更新日期:2009-05-01 00:00:00
abstract::This paper presents the results of a study of linkage disequilibrium between five polymorphic enzyme genes located on chromosome 3 of D. melanogaster. Three sets of chromosomes were examined: two represented samples from successive years of the same natural population, and one came from a large laboratory population. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-02-01 00:00:00
abstract::A wheat (Triticum aestivum L. emend Thell) disomic addition line (2n = 6x = 44), SH1-152-2, with a pair of Elytrigia pontica (Podp.) Holub 2n = 10x = 70 [syn. Agropyron elongatum (Host) P.B.] chromosomes controlling blue aleurone color was crossed with a short-statured spring wheat ;Sonora 64' (T. aestivum). Isoline p...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::The 73AD salivary chromosome region of Drosophila melanogaster was subjected to mutational analysis in order to (1) generate a collection of chromosome breakpoints that would allow a correlation between the genetic, cytological and molecular maps of the region and (2) define the number and gross organization of comple...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::The forest tent caterpillar is polymorphic for two melanic genes affecting wing color of moths. These are the first genetically determined morphological traits reported for the genus. Dark (D) is a sex-limited, autosomal dominant with a phenotype of dark brown males. Frequencies in population samples varied from 8 to ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Aspergillus nidulans is an important experimental organism, and it is a model organism for the genus Aspergillus that includes serious pathogens as well as commercially important organisms. Gene targeting by homologous recombination during transformation is possible in A. nidulans, but the frequency of correct gene ta...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.052563
更新日期:2006-03-01 00:00:00
abstract::The aim of many genetic studies is to locate the genomic regions (called quantitative trait loci, QTL) that contribute to variation in a quantitative trait (such as body weight). Confidence intervals for the locations of QTL are particularly important for the design of further experiments to identify the gene or genes...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.061549
更新日期:2006-09-01 00:00:00
abstract::Rp1 is a complex rust resistance locus of maize. The HRp1-D haplotype is composed of Rp1-D and eight paralogues, seven of which also code for predicted nucleotide binding site-leucine rich repeat (NBS-LRR) proteins similar to the Rp1-D gene. The paralogues are polymorphic (DNA identities 91-97%), especially in the C-t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::Comparison of population differentiation in neutral marker genes and in genes coding quantitative traits by means of F(ST) and Q(ST) indexes has become commonplace practice. While the properties and estimation of F(ST) have been the subject of much interest, little is known about the precision and possible bias in Q(S...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.044545
更新日期:2005-11-01 00:00:00
abstract::Meiosis was analyzed cytogenetically in autotetraploids of Arabidopsis, including both established lines and newly generated autotetraploid plants. Fluorescent in situ hybridization with 5S and 45S rDNA probes was used to identify the different chromosomes at metaphase I of meiosis. Multivalents were observed frequent...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Antisuppressor mutations in the eEF1A gene of Podospora anserina were previously shown to impair ascospore formation, to drastically increase life span, and to permit the development of the Crippled Growth degenerative process. Here, we show that eEF1A controls ascospore formation through accuracy level maintenance. E...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Preliminary studies with restriction fragment length polymorphisms of mitochondrial DNA (mtDNA) in natural populations of Drosophila melanogaster revealed considerable variation in terms of nucleotide sequence and overall size. In this report we present data from more isofemale lines and more restriction enzymes, and ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::Forty-one stocks from 30 Drosophila species were surveyed for Wolbachia infection using PCR technology. D. sechellia and two strains of D. auraria were found to be infected and were tested for the expression of cytoplasmic incompatibility, along with D. ananassae and D. melanogaster strains, which are already known to...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::The L-shaped distribution of estimated QTL effects (R(2)) has long been reported. We recently showed that a metabolic mechanism could account for this phenomenon. But other nonexclusive genetic or nongenetic causes may contribute to generate such a distribution. Using analysis and simulations of an additive genetic mo...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::We have characterized tandem duplications in the rII regions of phage T4. The rII deletion r1589 blocks only the function of the rIIA cistron, although it extends into the B cistron. Another rII deletion, r1236, blocks the function of the rIIB cistron and overlaps r1589. When a cross is made between r1589 and r1236, t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::The T-box genes comprise an ancient family of putative transcription factors conserved across species as divergent as Mus musculus and Caenorhabditis elegans. All T-box gene products are characterized by a novel 174-186-amino acid DNA binding domain called the T-box that was first discovered in the polypeptide product...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::We use the ancestral influence graph (AIG) for a two-locus, two-allele selection model in the limit of a large population size to obtain an analytic approximation for the probability of ultimate fixation of a single mutant allele A. We assume that this new mutant is introduced at a given locus into a finite population...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.136309
更新日期:2012-02-01 00:00:00
abstract::AFLP markers are becoming one of the most popular tools for genetic analysis in the fields of evolutionary genetics and ecology and conservation of genetic resources. The technique combines a high-information content and fidelity with the possibility of carrying out genomewide scans. However, a potential problem with ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.083246
更新日期:2008-05-01 00:00:00
abstract::A study of DNA polymorphism and divergence was conducted for the cytosolic phosphoglucose isomerase (PGI:E.C.5.3.1.9) gene of five species of the mustard genus Leavenworthia: Leavenworthia stylosa, L. alabamica, L. crassa, L. uniflora, and L. torulosa. Sequences of an internal 2.3-kb PgiC gene region spanning exons 6-...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::I factors in Drosophila melanogaster are non-LTR retrotransposons that transpose at very high frequencies in the germ line of females resulting from crosses between reactive females (devoid of active I factors) and inducer males (containing active I factors). Constructs containing I factor ORF1 under the control of th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::We develop a maximum penalized-likelihood (MPL) method to estimate the fitnesses of amino acids and the distribution of selection coefficients (S = 2Ns) in protein-coding genes from phylogenetic data. This improves on a previous maximum-likelihood method. Various penalty functions are used to penalize extreme estimate...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.162263
更新日期:2014-05-01 00:00:00
abstract::Thirty-five nucleotide polymorphisms were found in a 21.5-kbp region including the Est6 locus among 42 isoallelic lines extracted from a single natural population of Drosophila melanogaster. The heterozygosity per nucleotide pair was estimated to be 0.010 overall, but was lower in sequences hybridizing to transcripts ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00