Abstract：
:Three-dimensional (3D) cell cultures produce more in vivo-like multicellular structures such as spheroids that cannot be obtained in two-dimensional (2D) cell cultures. Thus, they are increasingly employed as models for cancer and drug research, as well as tissue engineering. It has proven challenging to stabilize spheroid architectures for detailed morphological examination. Here we overcome this issue using a silica bioreplication (SBR) process employed on spheroids formed from human pluripotent stem cells (hPSCs) and hepatocellular carcinoma HepG2 cells cultured in the nanofibrillar cellulose (NFC) hydrogel. The cells in the spheroids are more round and tightly interacting with each other than those in 2D cultures, and they develop microvilli-like structures on the cell membranes as seen in 2D cultures. Furthermore, SBR preserves extracellular matrix-like materials and cellular proteins. These findings provide the first evidence of intact hPSC spheroid architectures and similar fine structures to 2D-cultured cells, providing a pathway to enable our understanding of morphogenesis in 3D cultures.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lou YR,Kanninen L,Kaehr B,Townson JL,Niklander J,Harjumäki R,Jeffrey Brinker C,Yliperttula Mdoi
10.1038/srep13635subject
Has Abstractpub_date
2015-09-01 00:00:00pages
13635issn
2045-2322pii
srep13635journal_volume
5pub_type
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