Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium.

abstract：BACKGROUND:L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS:In this study, Corynebacterium gl...

journal_title：Microbial cell factories

authors： Zhang B,Yu M,Zhou Y,Li Y,Ye BC

更新日期：2017-09-22 00:00:00

abstract：:Industrial development may result in the increase of environmental risks. The enzymatic transformation of polluting compounds to less toxic or even innocuous products is an alternative to their complete removal. In this regard, a number of different redox enzymes are able to transform a wide variety of toxic pollutant...

journal_title：Microbial cell factories

authors： Garcia-Arellano H,Alcalde M,Ballesteros A

更新日期：2004-08-02 00:00:00

abstract：BACKGROUND:The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose....

journal_title：Microbial cell factories

authors： Ying H,Tao S,Wang J,Ma W,Chen K,Wang X,Ouyang P

更新日期：2017-03-27 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potenti...

journal_title：Microbial cell factories

authors： Yang YJ,Wang Y,Li ZF,Gong Y,Zhang P,Hu WC,Sheng DH,Li YZ

更新日期：2017-08-16 00:00:00

abstract：BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...

journal_title：Microbial cell factories

authors： Nowrouzi B,Li RA,Walls LE,d'Espaux L,Malcı K,Liang L,Jonguitud-Borrego N,Lerma-Escalera AI,Morones-Ramirez JR,Keasling JD,Rios-Solis L

更新日期：2020-11-02 00:00:00

abstract：BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...

journal_title：Microbial cell factories

authors： Papi RM,Chaitidou SA,Trikka FA,Kyriakidis DA

更新日期：2005-12-14 00:00:00

abstract：BACKGROUND:Saccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase (PDH) complex ex...

journal_title：Microbial cell factories

authors： Zhang Y,Su M,Qin N,Nielsen J,Liu Z

更新日期：2020-12-10 00:00:00

abstract：BACKGROUND:Ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. These compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. Bio...

journal_title：Microbial cell factories

authors： Nielsen CA,Folly C,Hatsch A,Molt A,Schröder H,O'Connor SE,Naesby M

更新日期：2014-08-12 00:00:00

abstract：BACKGROUND:The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis. RESULTS:The presence of preformed biomass precursor mol...

journal_title：Microbial cell factories

authors： Li Z,Nimtz M,Rinas U

更新日期：2014-03-23 00:00:00

abstract：:The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community easy and versatile access to the sequence, two web-sites have been installed as a resource for genomic sequence, gene and protein...

journal_title：Microbial cell factories

authors： Mattanovich D,Callewaert N,Rouzé P,Lin YC,Graf A,Redl A,Tiels P,Gasser B,De Schutter K

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmf...

journal_title：Microbial cell factories

authors： Kawai Y,Noda S,Ogino C,Takeshima Y,Okai N,Tanaka T,Kondo A

更新日期：2013-05-07 00:00:00

abstract：BACKGROUND:The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-...

journal_title：Microbial cell factories

authors： Huo YY,Jian SL,Cheng H,Rong Z,Cui HL,Xu XW

更新日期：2018-01-30 00:00:00

abstract：BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...

journal_title：Microbial cell factories

authors： Hossain AH,Li A,Brickwedde A,Wilms L,Caspers M,Overkamp K,Punt PJ

更新日期：2016-07-28 00:00:00

abstract：BACKGROUND:Understanding the metabolic mechanism of sterols to produce valuable steroid intermediates in mycobacterium by a noncoding small RNA (sRNA) view is still limited. In the work, RNA-seq was implemented to investigate the noncoding transcriptome of Mycobacterium neoaurum (Mn) in the transformation process of st...

journal_title：Microbial cell factories

authors： Liu M,Zhu ZT,Tao XY,Wang FQ,Wei DZ

更新日期：2016-04-25 00:00:00

abstract：BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...

journal_title：Microbial cell factories

authors： Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

更新日期：2015-04-17 00:00:00

abstract：:All of humans and other mammalian species are colonized by some types of microorganisms such as bacteria, archaea, unicellular eukaryotes like fungi and protozoa, multicellular eukaryotes like helminths, and viruses, which in whole are called microbiota. These microorganisms have multiple different types of interactio...

journal_title：Microbial cell factories

authors： Baghbani T,Nikzad H,Azadbakht J,Izadpanah F,Haddad Kashani H

更新日期：2020-11-26 00:00:00

abstract：BACKGROUND:Under N-limiting conditions, Saccharomyces cerevisiae strains display a substantial variability in their biomass yield from consumed nitrogen -in particular wine yeasts exhibit high growth abilities- that is correlated with their capacity to complete alcoholic fermentation, a trait of interest for fermented ...

journal_title：Microbial cell factories

authors： Crépin L,Sanchez I,Nidelet T,Dequin S,Camarasa C

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, ...

journal_title：Microbial cell factories

authors： Yamaoka M,Osawa S,Morinaga T,Takenaka S,Yoshida K

更新日期：2011-09-07 00:00:00

abstract：BACKGROUND:Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermophilus α-amylase in B...

journal_title：Microbial cell factories

authors： Yao D,Su L,Li N,Wu J

更新日期：2019-04-11 00:00:00

abstract：BACKGROUND:Recent years have seen a huge growth in the market of industrial yeasts with the need for strains affording better performance or to be used in new applications. Stress tolerance of commercial Saccharomyces cerevisiae yeasts is, without doubt, a trait that needs improving. Such trait is, however, complex, an...

journal_title：Microbial cell factories

authors： Pérez-Torrado R,Panadero J,Hernández-López MJ,Prieto JA,Randez-Gil F

更新日期：2010-07-13 00:00:00

abstract：BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...

journal_title：Microbial cell factories

authors： Pyeon HR,Nah HJ,Kang SH,Choi SS,Kim ES

更新日期：2017-05-31 00:00:00

abstract：BACKGROUND:β-Ionone is a fragrant terpenoid that generates a pleasant floral scent and is used in diverse applications as a cosmetic and flavoring ingredient. A growing consumer desire for natural products has increased the market demand for natural β-ionone. To date, chemical extraction from plants remains the main ap...

journal_title：Microbial cell factories

authors： Czajka JJ,Nathenson JA,Benites VT,Baidoo EEK,Cheng Q,Wang Y,Tang YJ

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. RESULTS:In this stu...

journal_title：Microbial cell factories

authors： Zhang H,Liu Q,Cao Y,Feng X,Zheng Y,Zou H,Liu H,Yang J,Xian M

更新日期：2014-02-10 00:00:00

abstract：:BACKGROUND: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence signal generated b...

journal_title：Microbial cell factories

authors： Rautio J,Barken KB,Lahdenperä J,Breitenstein A,Molin S,Neubauer P

更新日期：2003-04-28 00:00:00

abstract：BACKGROUND:Intracellular metabolism of glucocorticoid hormones plays an important role in the pathogenesis of metabolic syndrome and regulates, among many physiological processes, collagen metabolism in skin. At the peripheral level the concentration of active glucocorticoids is mainly regulated by the 11β-hydroxystero...

journal_title：Microbial cell factories

authors： Vanella R,Callari R,Weston A,Heider H,Schwab MS,Kübler E

更新日期：2016-03-15 00:00:00

abstract：BACKGROUND:In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Alt...

journal_title：Microbial cell factories

authors： Shang W,Zhai Y,Ma Z,Yang G,Ding Y,Han D,Li J,Zhang H,Liu J,Wang PG,Liu XW,Chen M

更新日期：2016-08-11 00:00:00

abstract：BACKGROUND:D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. ...

journal_title：Microbial cell factories

authors： Feng X,Jiang L,Han X,Liu X,Zhao Z,Liu H,Xian M,Zhao G

更新日期：2017-11-21 00:00:00

abstract：:L-arginine (ARG) is an important amino acid for both medicinal and industrial applications. For almost six decades, the research has been going on for its improved industrial level production using different microorganisms. While the initial approaches involved random mutagenesis for increased tolerance to ARG and con...

journal_title：Microbial cell factories

authors： Shin JH,Lee SY

更新日期：2014-12-03 00:00:00

abstract：: Microbes utilize enzymes to perform a variety of functions. Enzymes are biocatalysts working as highly efficient machines at the molecular level. In the past, enzymes have been viewed as static entities and their function has been explained on the basis of direct structural interactions between the enzyme and the sub...

journal_title：Microbial cell factories

authors： Agarwal PK

更新日期：2006-01-12 00:00:00

abstract：:Dunaliella salina is a unicellular green alga with a high α-linolenic acid (ALA) level, but a low eicosapentaenoic acid (EPA) level. In a previous analysis of the catalytic activity of delta 6 fatty acid desaturase (FADS6) from various species, FADS6 from Thalassiosira pseudonana (TpFADS6), a marine diatom, showed the...

journal_title：Microbial cell factories

authors： Shi H,Luo X,Wu R,Yue X

更新日期：2018-01-13 00:00:00