Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyase.

abstract：BACKGROUND:Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the maj...

journal_title：Microbial cell factories

authors： Yin H,Zhang R,Xia M,Bai X,Mou J,Zheng Y,Wang M

更新日期：2017-06-15 00:00:00

abstract：BACKGROUND:Streptomycetes produce a plethora of natural products including antibiotics and anticancer drugs, as well as many industrial enzymes. Their mycelial life style is a major bottleneck for industrial exploitation and over decades strain improvement programs have selected production strains with better growth pr...

journal_title：Microbial cell factories

authors： van Dissel D,Claessen D,Roth M,van Wezel GP

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:The application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, ...

journal_title：Microbial cell factories

authors： Raddadi N,Giacomucci L,Totaro G,Fava F

更新日期：2017-11-02 00:00:00

abstract：BACKGROUND:Numerous challenges remain to achieve industrially competitive space-time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development and biocatalyst selection; studies at conventional lab scale are ti...

journal_title：Microbial cell factories

authors： Kolmar JF,Thum O,Baganz F

更新日期：2017-10-10 00:00:00

abstract：BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...

journal_title：Microbial cell factories

authors： Li P,Sun H,Chen Z,Li Y,Zhu T

更新日期：2015-02-21 00:00:00

abstract：BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...

journal_title：Microbial cell factories

authors： Lehtinen T,Efimova E,Santala S,Santala V

更新日期：2018-02-08 00:00:00

abstract：BACKGROUND:β-Ionone is a fragrant terpenoid that generates a pleasant floral scent and is used in diverse applications as a cosmetic and flavoring ingredient. A growing consumer desire for natural products has increased the market demand for natural β-ionone. To date, chemical extraction from plants remains the main ap...

journal_title：Microbial cell factories

authors： Czajka JJ,Nathenson JA,Benites VT,Baidoo EEK,Cheng Q,Wang Y,Tang YJ

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose....

journal_title：Microbial cell factories

authors： Ying H,Tao S,Wang J,Ma W,Chen K,Wang X,Ouyang P

更新日期：2017-03-27 00:00:00

abstract：BACKGROUND:Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. To date, strain specific parameters, which are needed to set up feeding profiles for fed batch cultivations, are determined by time-consuming continuous cultures or consecutive ...

journal_title：Microbial cell factories

authors： Dietzsch C,Spadiut O,Herwig C

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...

journal_title：Microbial cell factories

authors： Yue YC,Yang BY,Lu J,Zhang SW,Liu L,Nassar K,Xu XX,Pang XY,Lv JP

更新日期：2020-11-23 00:00:00

abstract：BACKGROUND:Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an ...

journal_title：Microbial cell factories

authors： Dong W,Cai Y,Xu Z,Fu B,Chen Q,Cui Y,Ruan Z,Liang Y,Peng N,Zhao S

更新日期：2020-10-07 00:00:00

abstract：BACKGROUND:Acetic acid is a byproduct of Saccharomyces cerevisiae alcoholic fermentation. Together with high concentrations of ethanol and other toxic metabolites, acetic acid may contribute to fermentation arrest and reduced ethanol productivity. This weak acid is also a present in lignocellulosic hydrolysates, a high...

journal_title：Microbial cell factories

authors： Mira NP,Palma M,Guerreiro JF,Sá-Correia I

更新日期：2010-10-25 00:00:00

abstract：BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

journal_title：Microbial cell factories

authors： Upadhyay V,Singh A,Jha D,Singh A,Panda AK

更新日期：2016-06-08 00:00:00

abstract：BACKGROUND:Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose r...

journal_title：Microbial cell factories

authors： Cheng H,Wang S,Bilal M,Ge X,Zhang C,Fickers P,Cheng H

更新日期：2018-08-29 00:00:00

abstract：BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...

journal_title：Microbial cell factories

authors： Wu S,Gu W,Huang A,Li Y,Kumar M,Lim PE,Huan L,Gao S,Wang G

更新日期：2019-09-23 00:00:00

abstract：BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...

journal_title：Microbial cell factories

authors： Bruschi M,Krömer JO,Steen JA,Nielsen LK

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:4-Hydroxycinnamaldehydes are important intermediates in several secondary metabolism pathways, including those involved in the biosynthesis of phenolic acids, flavonoids, terpenoids and monolignols. They are also involved in the biosynthesis and degradation of lignins, which are important limiting factors du...

journal_title：Microbial cell factories

authors： Liu S,Qi Q,Chao N,Hou J,Rao G,Xie J,Lu H,Jiang X,Gai Y

更新日期：2015-08-12 00:00:00

abstract：BACKGROUND:Bacterial surface display is of interest in many applications, including live vaccine development, screening of protein libraries and the development of whole cell biocatalysts. The goal of this work was to understand which parameters result in production of large quantities of cells that at the same time ex...

journal_title：Microbial cell factories

authors： Gustavsson M,Bäcklund E,Larsson G

更新日期：2011-09-14 00:00:00

abstract：BACKGROUND:The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis. RESULTS:The presence of preformed biomass precursor mol...

journal_title：Microbial cell factories

authors： Li Z,Nimtz M,Rinas U

更新日期：2014-03-23 00:00:00

abstract：: The aim of this work is to present a critical review on slurry bioreactors (SB) and their application to bioremediation of soils and sediments polluted with recalcitrant and toxic compounds. The scope of the review encompasses the following subjects: (i) process fundamentals of SB and analysis of advantages and disad...

journal_title：Microbial cell factories

authors： Robles-González IV,Fava F,Poggi-Varaldo HM

更新日期：2008-02-29 00:00:00

abstract：BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...

journal_title：Microbial cell factories

authors： Pyeon HR,Nah HJ,Kang SH,Choi SS,Kim ES

更新日期：2017-05-31 00:00:00

abstract：BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...

journal_title：Microbial cell factories

authors： Scalcinati G,Partow S,Siewers V,Schalk M,Daviet L,Nielsen J

更新日期：2012-08-31 00:00:00

abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...

journal_title：Microbial cell factories

authors： Wu W,Xing L,Zhou B,Lin Z

更新日期：2011-02-15 00:00:00

abstract：BACKGROUND:L-tryptophan is an aromatic amino acid widely used in the food, chemical and pharmaceutical industries. In Escherichia coli, L-tryptophan is synthesized from phosphoenolpyruvate and erythrose 4-phosphate by enzymes in the shikimate pathway and L-tryptophan branch pathway, while L-serine and phosphoribosylpyr...

journal_title：Microbial cell factories

authors： Gu P,Yang F,Kang J,Wang Q,Qi Q

更新日期：2012-03-02 00:00:00

abstract：BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...

journal_title：Microbial cell factories

authors： Silva R,Aguiar TQ,Coelho E,Jiménez A,Revuelta JL,Domingues L

更新日期：2019-03-28 00:00:00

abstract：BACKGROUND:Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether prot...

journal_title：Microbial cell factories

authors： Cai D,Wang H,He P,Zhu C,Wang Q,Wei X,Nomura CT,Chen S

更新日期：2017-04-24 00:00:00

abstract：BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...

journal_title：Microbial cell factories

authors： Brink HG,Nicol W

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...

journal_title：Microbial cell factories

authors： Qiu C,Chen X,Rexida R,Shen Y,Qi Q,Bao X,Hou J

更新日期：2020-07-20 00:00:00

abstract：BACKGROUND:The Bacillus subtilis spore has long been used to display antigens and enzymes. Spore display can be accomplished by a recombinant and a non-recombinant approach, with the latter proved more efficient than the recombinant one. We used the non-recombinant approach to independently adsorb two thermophilic enzy...

journal_title：Microbial cell factories

authors： Mattossovich R,Iacono R,Cangiano G,Cobucci-Ponzano B,Isticato R,Moracci M,Ricca E

更新日期：2017-11-28 00:00:00

abstract：BACKGROUND:Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications deriv...

journal_title：Microbial cell factories

authors： Sun H,Zhao D,Xiong B,Zhang C,Bi C

更新日期：2016-08-24 00:00:00