Abstract:
BACKGROUND:The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis. RESULTS:The presence of preformed biomass precursor molecules in rich media such as Luria Bertani supported rapid growth concomitant to acetate formation and apparently unbalanced abundances of central metabolic pathway enzymes, e.g. high levels of lower glycolytic pathway enzymes as well as pyruvate dehydrogenase, and low levels of TCA cycle and high levels of the acetate forming enzymes Pta and AckA. The proteome of cells growing exponentially in glucose-supplemented mineral salt medium was dominated by enzymes of amino acid synthesis pathways, contained more balanced abundances of central metabolic pathway enzymes, and a lower portion of ribosomal and other translational proteins. Entry into stationary phase led to a reconstruction of the bacterial proteome by increasing e.g. the portion of proteins required for scavenging rare nutrients and general cell protection. This proteomic reconstruction during entry into stationary phase was more noticeable in cells growing in rich medium as they have a greater reservoir of recyclable proteins from the translational machinery. CONCLUSIONS:The proteomic comparison of cells growing exponentially in different media reflected the antagonistic and competitive regulation of central metabolic pathways through the global transcriptional regulators Cra, Crp, and ArcA. For example, the proteome of cells growing exponentially in rich medium was consistent with a dominating role of phosphorylated ArcA most likely a result from limitations in reoxidizing reduced quinones in the respiratory chain under these growth conditions. The proteomic alterations of exponentially growing cells into stationary phase cells were consistent with stringent-like and stationary phase responses and a dominating control through DksA-ppGpp and RpoS.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Li Z,Nimtz M,Rinas Udoi
10.1186/1475-2859-13-45subject
Has Abstractpub_date
2014-03-23 00:00:00pages
45issue
1issn
1475-2859pii
1475-2859-13-45journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Phasin (PhaP), a kind of polyhydroxyalkanoate (PHA) granule-associated proteins, has a role in controlling the properties of PHA granules surface, and is thought to have influence on PHA biosynthesis in PHA-producing bacteria. This study focused on the phaP1(Re) locus in Ralstonia eutropha as a site of chrom...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0380-8
更新日期:2015-11-23 00:00:00
abstract:: The aim of this work is to present a critical review on slurry bioreactors (SB) and their application to bioremediation of soils and sediments polluted with recalcitrant and toxic compounds. The scope of the review encompasses the following subjects: (i) process fundamentals of SB and analysis of advantages and disad...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-7-5
更新日期:2008-02-29 00:00:00
abstract:INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1036-2
更新日期:2018-12-03 00:00:00
abstract:BACKGROUND:p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmf...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-45
更新日期:2013-05-07 00:00:00
abstract:BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01466-2
更新日期:2020-11-23 00:00:00
abstract:BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-13-11
更新日期:2014-01-17 00:00:00
abstract:BACKGROUND:D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0822-6
更新日期:2017-11-21 00:00:00
abstract:BACKGROUND:The oleaginous yeast Yarrowia lipolytica is an organism of choice for the tailored production of various compounds such as biofuels or biopolymers. When properly engineered, it is capable of producing medium-chain-length polyhydroxyalkanoate (mcl-PHA), a biobased and biodegradable polymer that can be used as...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1140-y
更新日期:2019-05-31 00:00:00
abstract:BACKGROUND:Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol production. Although Z. mobilis is robustly resilient to certain abiotic stress such as ethanol, ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01406-0
更新日期:2020-07-20 00:00:00
abstract:BACKGROUND:Cholesterol, the precursor of all steroid hormones, is the most abundant steroid in vertebrates and exhibits highly hydrophobic properties, rendering it a difficult substrate for aqueous microbial biotransformations. In the present study, we developed a Bacillus megaterium based whole-cell system that allows...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0300-y
更新日期:2015-07-29 00:00:00
abstract:BACKGROUND:Microalgae has been concerned as a potential source of biodiesel in the recent years. However, it is costly to harvest microalgae as it is commonly cultured in water and the cells are too small to harvest. In order to reduce the cost of cultivation and harvesting, it is important to improve the biomass produ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0142-z
更新日期:2014-10-01 00:00:00
abstract::The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/s12934-020-1289-4
更新日期:2020-02-05 00:00:00
abstract:BACKGROUND:Bacillus subtilis is an important industrial workhorse applied in the production of many different commercially relevant proteins, especially enzymes. Virtually all of these proteins are secreted via the general secretion (Sec) pathway. Studies from different laboratories have demonstrated essential or non-e...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01315-2
更新日期:2020-02-28 00:00:00
abstract:BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01458-2
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:Thymosin α1 (Tα1), a 28-amino acid Nα-acetylated peptide, has a powerful general immunostimulating activity. Although biosynthesis is an attractive means of large-scale manufacture, to date, Tα1 can only be chemosynthesized because of two obstacles to its biosynthesis: the difficulties in expressing small pe...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-26
更新日期:2011-04-22 00:00:00
abstract::A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tiss...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-76
更新日期:2012-06-11 00:00:00
abstract:BACKGROUND:In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understood reasons, the produc...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-24
更新日期:2013-03-12 00:00:00
abstract:BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-4-35
更新日期:2005-12-14 00:00:00
abstract:BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-43
更新日期:2011-06-07 00:00:00
abstract:BACKGROUND:It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of practical interest for the heterologous protein production, PHB production, etc. Although the carbon and...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-8
更新日期:2010-01-27 00:00:00
abstract::Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/s12934-017-0669-x
更新日期:2017-04-04 00:00:00
abstract:BACKGROUND:Corynebacterium glutamicum is a well-known producer of various L-amino acids in industry. During the fermenting process, C. glutamicum unavoidably encounters oxidative stress due to a specific reactive oxygen species (ROS) produced by consistent adverse conditions. To combat the ROS, C. glutamicum has develo...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1048-y
更新日期:2018-12-26 00:00:00
abstract:BACKGROUND:Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In f...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-11
更新日期:2012-01-20 00:00:00
abstract:BACKGROUND:Transcriptional engineering has presented a strong ability of phenotypic improvement in microorganisms. However, it could not be directly applied to Actinoplanes teichomyceticus L-27 because of the paucity of endogenous transcription factors in the strain. In this study, exogenous transcription factors were ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-13-10
更新日期:2014-01-16 00:00:00
abstract:BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0504-9
更新日期:2016-06-08 00:00:00
abstract:BACKGROUND:A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0837-z
更新日期:2017-12-12 00:00:00
abstract:BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0708-7
更新日期:2017-05-31 00:00:00
abstract:BACKGROUND:Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0936-5
更新日期:2018-06-07 00:00:00
abstract:BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0977-9
更新日期:2018-08-21 00:00:00
abstract:BACKGROUND:Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives i...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-138
更新日期:2012-10-24 00:00:00