Abstract：
BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gamma-aminobutyrate (GABA), a versatile bulk chemical used in food and pharmaceutical industry, is also used as a key monomer for nylon 4. GABA can be biologically produced by decarboxylation of glutamate. RESULTS:In this study, we examined high glutamate-producing Corynebacterium glutamicum strains as hosts for enhanced production of GABA from glucose and xylose as carbon sources. An Escherichia coli gadB mutant with a broad pH range of activity and E. coli xylAB genes were expressed under the control of a synthetic H36 promoter. When empty fruit bunch (EFB) solution was used as carbon source (45 g/L glucose and 5 g/L xylose), 12.54 ± 0.07 g/L GABA was produced by recombinant C. glutamicum H36GD1852 expressing E. coli gadB mutant gene and xylAB genes. Batch fermentation of the same strain resulted in the production of 35.47 g/L of GABA when EFB solution was added to support 90 g/L glucose and 10 g/L xylose. CONCLUSIONS:This is the first report of GABA production by recombinant C. glutamicum strains from co-utilization of glucose and xylose from EFB solution. Recombinant C. glutamicum strains developed in this study should be useful for an efficient and sustainable production of GABA from lignocellulosic biomasses.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Baritugo KA,Kim HT,David Y,Khang TU,Hyun SM,Kang KH,Yu JH,Choi JH,Song JJ,Joo JC,Park SJdoi
10.1186/s12934-018-0977-9subject
Has Abstractpub_date
2018-08-21 00:00:00pages
129issue
1issn
1475-2859pii
10.1186/s12934-018-0977-9journal_volume
17pub_type
杂志文章abstract：BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...
journal_title：Microbial cell factories
pub_type： 杂志文章
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更新日期：2015-11-05 00:00:00
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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doi：10.1186/1475-2859-12-111
更新日期：2013-11-16 00:00:00
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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更新日期：2012-05-09 00:00:00
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journal_title：Microbial cell factories
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更新日期：2011-08-11 00:00:00
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journal_title：Microbial cell factories
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更新日期：2016-08-02 00:00:00
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更新日期：2015-08-12 00:00:00
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journal_title：Microbial cell factories
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doi：10.1186/s12934-018-0918-7
更新日期：2018-05-18 00:00:00
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journal_title：Microbial cell factories
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更新日期：2019-03-18 00:00:00
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journal_title：Microbial cell factories
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更新日期：2011-12-15 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2017-04-04 00:00:00
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journal_title：Microbial cell factories
pub_type： 社论
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更新日期：2004-08-02 00:00:00
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更新日期：2010-03-10 00:00:00
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journal_title：Microbial cell factories
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