Carbohydrate catabolic flexibility in the mammalian intestinal commensal Lactobacillus ruminis revealed by fermentation studies aligned to genome annotations.

abstract：BACKGROUND:Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of medical interest, as it is "Generally Recognized As Safe". Furthermore, as L. lactis naturally secretes only one major endoge...

journal_title：Microbial cell factories

authors： Samazan F,Rokbi B,Seguin D,Telles F,Gautier V,Richarme G,Chevret D,Varela PF,Velours C,Poquet I

更新日期：2015-07-16 00:00:00

abstract：BACKGROUND:Despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. Shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. Thu...

journal_title：Microbial cell factories

authors： Flitsch D,Ladner T,Lukacs M,Büchs J

更新日期：2016-02-24 00:00:00

abstract：: The aim of this work is to present a critical review on slurry bioreactors (SB) and their application to bioremediation of soils and sediments polluted with recalcitrant and toxic compounds. The scope of the review encompasses the following subjects: (i) process fundamentals of SB and analysis of advantages and disad...

journal_title：Microbial cell factories

authors： Robles-González IV,Fava F,Poggi-Varaldo HM

更新日期：2008-02-29 00:00:00

abstract：BACKGROUND:Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives i...

journal_title：Microbial cell factories

authors： Peifer S,Barduhn T,Zimmet S,Volmer DA,Heinzle E,Schneider K

更新日期：2012-10-24 00:00:00

abstract：INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...

journal_title：Microbial cell factories

authors： Haq SF,Shanbhag AP,Karthikeyan S,Hassan I,Thanukrishnan K,Ashok A,Sukumaran S,Ramaswamy S,Bharatham N,Datta S,Samant S,Katagihallimath N

更新日期：2018-12-03 00:00:00

abstract：BACKGROUND:The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation. In this context, the bacterium Thermus thermophilus HB27 is a metal tolerant thermophile containing a set of genes involved in arsenic resistance which, differen...

journal_title：Microbial cell factories

authors： Antonucci I,Gallo G,Limauro D,Contursi P,Ribeiro AL,Blesa A,Berenguer J,Bartolucci S,Fiorentino G

更新日期：2018-05-18 00:00:00

abstract：BACKGROUND:The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-...

journal_title：Microbial cell factories

authors： Huo YY,Jian SL,Cheng H,Rong Z,Cui HL,Xu XW

更新日期：2018-01-30 00:00:00

abstract：:Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...

journal_title：Microbial cell factories

authors： Villaverde A

更新日期：2010-07-05 00:00:00

abstract：BACKGROUND:Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, constituting direct repeats (D...

journal_title：Microbial cell factories

authors： Ding T,Huang C,Liang Z,Ma X,Wang N,Huo YX

更新日期：2020-03-10 00:00:00

abstract：BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...

journal_title：Microbial cell factories

authors： Silva R,Aguiar TQ,Coelho E,Jiménez A,Revuelta JL,Domingues L

更新日期：2019-03-28 00:00:00

abstract：BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...

journal_title：Microbial cell factories

authors： Lehtinen T,Efimova E,Santala S,Santala V

更新日期：2018-02-08 00:00:00

abstract：BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...

journal_title：Microbial cell factories

authors： Qiu C,Chen X,Rexida R,Shen Y,Qi Q,Bao X,Hou J

更新日期：2020-07-20 00:00:00

abstract：BACKGROUND:Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hos...

journal_title：Microbial cell factories

authors： Jin H,Jeong Y,Yoo SH,Johnston TV,Ku S,Ji GE

更新日期：2019-06-13 00:00:00

abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...

journal_title：Microbial cell factories

authors： Wu W,Xing L,Zhou B,Lin Z

更新日期：2011-02-15 00:00:00

abstract：BACKGROUND:2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in human milk which protects against the infection of enteric pathogens. Because 2-FL can be synthesized through the enzymatic fucosylation of lactose with guanosine 5'-diphosphate (GDP)-l-fucose by α-1,2-fucosyltransferase (FucT2), an 2-FL pro...

journal_title：Microbial cell factories

authors： Lee WH,Pathanibul P,Quarterman J,Jo JH,Han NS,Miller MJ,Jin YS,Seo JH

更新日期：2012-04-30 00:00:00

abstract：BACKGROUND:Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database. Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, s...

journal_title：Microbial cell factories

authors： Yang Y,Yang J,Wang R,Liu J,Zhang Y,Liu L,Wang F,Yuan H

更新日期：2019-09-21 00:00:00

abstract：BACKGROUND:Most bacteria can use various compounds as carbon sources. These carbon sources can be either co-metabolized or sequentially metabolized, where the latter phenomenon typically occurs as catabolite repression. From the practical application point of view of utilizing lignocellulose for the production of biofu...

journal_title：Microbial cell factories

authors： Yao R,Hirose Y,Sarkar D,Nakahigashi K,Ye Q,Shimizu K

更新日期：2011-08-11 00:00:00

abstract：BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...

journal_title：Microbial cell factories

authors： Xue YP,Zeng H,Jin XL,Liu ZQ,Zheng YG

更新日期：2016-09-22 00:00:00

abstract：BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...

journal_title：Microbial cell factories

authors： Wons E,Koscielniak D,Szadkowska M,Sektas M

更新日期：2018-09-21 00:00:00

abstract：BACKGROUND:Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether prot...

journal_title：Microbial cell factories

authors： Cai D,Wang H,He P,Zhu C,Wang Q,Wei X,Nomura CT,Chen S

更新日期：2017-04-24 00:00:00

abstract：BACKGROUND:Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest...

journal_title：Microbial cell factories

authors： Vogt CM,Schraner EM,Aguilar C,Eichwald C

更新日期：2016-08-11 00:00:00

abstract：BACKGROUND:Transcriptional engineering has presented a strong ability of phenotypic improvement in microorganisms. However, it could not be directly applied to Actinoplanes teichomyceticus L-27 because of the paucity of endogenous transcription factors in the strain. In this study, exogenous transcription factors were ...

journal_title：Microbial cell factories

authors： Wang H,Yang L,Wu K,Li G

更新日期：2014-01-16 00:00:00

abstract：BACKGROUND:Green microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entiti...

journal_title：Microbial cell factories

authors： Sivakumar G,Jeong K,Lay JO Jr

更新日期：2014-06-03 00:00:00

abstract：BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...

journal_title：Microbial cell factories

authors： van der Straat L,Vernooij M,Lammers M,van den Berg W,Schonewille T,Cordewener J,van der Meer I,Koops A,de Graaff LH

更新日期：2014-01-17 00:00:00

abstract：BACKGROUND:The metabolic capabilities of acetogens to ferment a wide range of sugars, to grow autotrophically on H2/CO2, and more importantly on synthesis gas (H2/CO/CO2) make them very attractive candidates as production hosts for biofuels and biocommodities. Acetogenic metabolism is considered one of the earliest mod...

journal_title：Microbial cell factories

authors： Nagarajan H,Sahin M,Nogales J,Latif H,Lovley DR,Ebrahim A,Zengler K

更新日期：2013-11-25 00:00:00

abstract：BACKGROUND:The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics and a work-intense s...

journal_title：Microbial cell factories

authors： Schwarzhans JP,Wibberg D,Winkler A,Luttermann T,Kalinowski J,Friehs K

更新日期：2016-05-20 00:00:00

abstract：BACKGROUND:Cinnamyl alcohol is not only a kind of flavoring agent and fragrance, but also a versatile chemical applied in the production of various compounds. At present, the preparation of cinnamyl alcohol depends on plant extraction and chemical synthesis, which have several drawbacks, including limited scalability, ...

journal_title：Microbial cell factories

authors： Zhang C,Xu Q,Hou H,Wu J,Zheng Z,Ouyang J

更新日期：2020-08-12 00:00:00

abstract：:L-arginine (ARG) is an important amino acid for both medicinal and industrial applications. For almost six decades, the research has been going on for its improved industrial level production using different microorganisms. While the initial approaches involved random mutagenesis for increased tolerance to ARG and con...

journal_title：Microbial cell factories

authors： Shin JH,Lee SY

更新日期：2014-12-03 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

journal_title：Microbial cell factories

authors： Menzella HG

更新日期：2011-03-03 00:00:00