Cooperation of hydrolysis modes among xylanases reveals the mechanism of hemicellulose hydrolysis by Penicillium chrysogenum P33.

abstract：BACKGROUND:Recombinant protein expression in bacteria often leads to the formation of intracellular insoluble protein deposits, a major bottleneck for the production of soluble and active products. However, in recent years, these bacterial protein aggregates, commonly known as inclusion bodies (IBs), have been shown to...

journal_title：Microbial cell factories

authors： Gil-Garcia M,Navarro S,Ventura S

更新日期：2020-06-01 00:00:00

abstract：BACKGROUND:Microalgae has been concerned as a potential source of biodiesel in the recent years. However, it is costly to harvest microalgae as it is commonly cultured in water and the cells are too small to harvest. In order to reduce the cost of cultivation and harvesting, it is important to improve the biomass produ...

journal_title：Microbial cell factories

authors： Chen X,Liu T,Wang Q

更新日期：2014-10-01 00:00:00

abstract：BACKGROUND:A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS:Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. O...

journal_title：Microbial cell factories

authors： Faijes M,Mars AE,Smid EJ

更新日期：2007-08-20 00:00:00

abstract：BACKGROUND:Targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the N-terminus of a secretory protein. These signal sequences usually contain an N-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signa...

journal_title：Microbial cell factories

authors： Yarimizu T,Nakamura M,Hoshida H,Akada R

更新日期：2015-02-14 00:00:00

abstract：BACKGROUND:Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward...

journal_title：Microbial cell factories

authors： Faria C,Borges N,Rocha I,Santos H

更新日期：2018-11-16 00:00:00

abstract：BACKGROUND:Numerous challenges remain to achieve industrially competitive space-time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development and biocatalyst selection; studies at conventional lab scale are ti...

journal_title：Microbial cell factories

authors： Kolmar JF,Thum O,Baganz F

更新日期：2017-10-10 00:00:00

abstract：BACKGROUND:Fine-tuning the aeration for cultivations when oxygen-limited conditions are demanded (such as the production of vaccines, isobutanol, 2-3 butanediol, acetone, and bioethanol) is still a challenge in the area of bioreactor automation and advanced control. In this work, an innovative control strategy based on...

journal_title：Microbial cell factories

authors： Mesquita TJB,Sargo CR,Fuzer JR Neto,Paredes SAH,Giordano RC,Horta ACL,Zangirolami TC

更新日期：2019-09-04 00:00:00

abstract：BACKGROUND:Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. RESULTS:In this stu...

journal_title：Microbial cell factories

authors： Zhang H,Liu Q,Cao Y,Feng X,Zheng Y,Zou H,Liu H,Yang J,Xian M

更新日期：2014-02-10 00:00:00

abstract：BACKGROUND:β-Ionone is a fragrant terpenoid that generates a pleasant floral scent and is used in diverse applications as a cosmetic and flavoring ingredient. A growing consumer desire for natural products has increased the market demand for natural β-ionone. To date, chemical extraction from plants remains the main ap...

journal_title：Microbial cell factories

authors： Czajka JJ,Nathenson JA,Benites VT,Baidoo EEK,Cheng Q,Wang Y,Tang YJ

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...

journal_title：Microbial cell factories

authors： Bruschi M,Krömer JO,Steen JA,Nielsen LK

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:(2S)-Pinocembrin is a chiral flavanone with versatile pharmacological and biological activities. Its health-promoting effects have spurred on research effects on the microbial production of (2S)-pinocembrin. However, an often-overlooked salient feature in the analysis of microbial (2S)-pinocembrin is its chi...

journal_title：Microbial cell factories

authors： Guo L,Chen X,Li LN,Tang W,Pan YT,Kong JQ

更新日期：2016-02-04 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potenti...

journal_title：Microbial cell factories

authors： Yang YJ,Wang Y,Li ZF,Gong Y,Zhang P,Hu WC,Sheng DH,Li YZ

更新日期：2017-08-16 00:00:00

abstract：BACKGROUND:In spite of the substantial metabolic engineering effort previously devoted to the development of Saccharomyces cerevisiae strains capable of fermenting both the hexose and pentose sugars present in lignocellulose hydrolysates, the productivity of reported strains for conversion of the naturally most abundan...

journal_title：Microbial cell factories

authors： Krahulec S,Petschacher B,Wallner M,Longus K,Klimacek M,Nidetzky B

更新日期：2010-03-10 00:00:00

abstract：BACKGROUND:The efficiency of biotechnological production processes depends on selecting the best performing microbial strain and the optimal cultivation conditions. Thus, many experiments have to be conducted, which conflicts with the demand to speed up drug development processes. Consequently, there is a great need fo...

journal_title：Microbial cell factories

authors： Funke M,Buchenauer A,Mokwa W,Kluge S,Hein L,Müller C,Kensy F,Büchs J

更新日期：2010-11-13 00:00:00

abstract：BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...

journal_title：Microbial cell factories

authors： Scalcinati G,Partow S,Siewers V,Schalk M,Daviet L,Nielsen J

更新日期：2012-08-31 00:00:00

abstract：BACKGROUND:Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing attention, but there were no reports of successful implementation. Escherichia coli has been engin...

journal_title：Microbial cell factories

authors： Wang W,He P,Zhao D,Ye L,Dai L,Zhang X,Sun Y,Zheng J,Bi C

更新日期：2019-07-05 00:00:00

abstract：:L-arginine (ARG) is an important amino acid for both medicinal and industrial applications. For almost six decades, the research has been going on for its improved industrial level production using different microorganisms. While the initial approaches involved random mutagenesis for increased tolerance to ARG and con...

journal_title：Microbial cell factories

authors： Shin JH,Lee SY

更新日期：2014-12-03 00:00:00

abstract：BACKGROUND:Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest...

journal_title：Microbial cell factories

authors： Vogt CM,Schraner EM,Aguilar C,Eichwald C

更新日期：2016-08-11 00:00:00

abstract：BACKGROUND:Streptomyces venezuelae ATCC 10712 produces antibiotics chloramphenicol (Cml) and jadomycin (Jad) in response to nutrient limitation and ethanol shock (ES), respectively. Biosynthesis of Cml and Jad was shown to be reciprocally regulated via the action of regulatory proteins JadR1 and JadR2 encoded by the ja...

journal_title：Microbial cell factories

authors： Sekurova ON,Zhang J,Kristiansen KA,Zotchev SB

更新日期：2016-05-20 00:00:00

abstract：: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been th...

journal_title：Microbial cell factories

authors： Gasser B,Saloheimo M,Rinas U,Dragosits M,Rodríguez-Carmona E,Baumann K,Giuliani M,Parrilli E,Branduardi P,Lang C,Porro D,Ferrer P,Tutino ML,Mattanovich D,Villaverde A

更新日期：2008-04-04 00:00:00

abstract：BACKGROUND:Chinese Nong-flavor (NF) liquor is continuously and stably produced by solid-state fermentation technology for 1000 years, resulting in enrichment of special microbial community and enzymes system in its starter. Based on traditional culture-dependent methods, these functional enzymes are hardly obtained. Ac...

journal_title：Microbial cell factories

authors： Yi Z,Fang Y,He K,Liu D,Luo H,Zhao D,He H,Jin Y,Zhao H

更新日期：2018-02-22 00:00:00

abstract：BACKGROUND:Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a vola...

journal_title：Microbial cell factories

authors： Duell ER,D'Agostino PM,Shapiro N,Woyke T,Fuchs TM,Gulder TAM

更新日期：2019-02-07 00:00:00

abstract：:Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...

journal_title：Microbial cell factories

authors： Cai G,Zheng W,Yang X,Zhang B,Zheng T

更新日期：2014-05-24 00:00:00

abstract：BACKGROUND:The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose....

journal_title：Microbial cell factories

authors： Ying H,Tao S,Wang J,Ma W,Chen K,Wang X,Ouyang P

更新日期：2017-03-27 00:00:00

abstract：BACKGROUND:L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS:In this study, Corynebacterium gl...

journal_title：Microbial cell factories

authors： Zhang B,Yu M,Zhou Y,Li Y,Ye BC

更新日期：2017-09-22 00:00:00

abstract：BACKGROUND:Cinnamyl alcohol is not only a kind of flavoring agent and fragrance, but also a versatile chemical applied in the production of various compounds. At present, the preparation of cinnamyl alcohol depends on plant extraction and chemical synthesis, which have several drawbacks, including limited scalability, ...

journal_title：Microbial cell factories

authors： Zhang C,Xu Q,Hou H,Wu J,Zheng Z,Ouyang J

更新日期：2020-08-12 00:00:00

abstract：BACKGROUND:Under aerobic conditions, acetic acid is the major byproduct produced by E. coli during the fermentation. And acetic acid is detrimental to cell growth as it destroys transmembrane pH gradients. Hence, how to reduce the production of acetic acid and how to utilize it as a feedstock are of intriguing interest...

journal_title：Microbial cell factories

authors： Yang J,Nie Q

更新日期：2016-05-05 00:00:00

abstract：BACKGROUND:Cell surface display of recombinant proteins has become a powerful tool for biotechnology and biomedical applications. As a model eukaryotic microorganism, Saccharomyces cerevisiae is an ideal candidate for surface display of heterologous proteins. However, the frequently used commercial yeast surface displa...

journal_title：Microbial cell factories

authors： Yang X,Tang H,Song M,Shen Y,Hou J,Bao X

更新日期：2019-05-18 00:00:00

abstract：BACKGROUND:Zygosaccharomyces bailii is a diploid budding yeast still poorly characterized, but widely recognised as tolerant to several stresses, most of which related to industrial processes of production. Because of that, it would be very interesting to develop its ability as a cell factory. Gas1p is a beta-1,3-gluca...

journal_title：Microbial cell factories

authors： Passolunghi S,Riboldi L,Dato L,Porro D,Branduardi P

更新日期：2010-01-26 00:00:00

abstract：BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...

journal_title：Microbial cell factories

authors： Yang J,Son JH,Kim H,Cho S,Na JG,Yeon YJ,Lee J

更新日期：2019-10-10 00:00:00