当前位置： SCI文献检索 > Microbial Cell Factories期刊下所有文献 > Combination of uniform design with artificial neural network coupling genetic algorithm: an effective way to obtain high yield of biomass and algicidal compound of a novel HABs control actinomycete.

Combination of uniform design with artificial neural network coupling genetic algorithm: an effective way to obtain high yield of biomass and algicidal compound of a novel HABs control actinomycete.

Abstract：

:Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, we developed a protocol to increase the yield of both biomass and algicidal compound present in a novel algicidal actinomycete Streptomyces alboflavus RPS, which kills Phaeocystis globosa. To overcome the problem in algicidal compound quantification, we chose algicidal ratio as the index and used artificial neural network to fit the data, which was appropriate for this nonlinear situation. In this protocol, we firstly determined five main influencing factors through single factor experiments and generated the multifactorial experimental groups with a U15(155) uniform-design-table. Then, we used the traditional quadratic polynomial stepwise regression model and an accurate, fully optimized BP-neural network to simulate the fermentation. Optimized with genetic algorithm and verified using experiments, we successfully increased the algicidal ratio of the fermentation broth by 16.90% and the dry mycelial weight by 69.27%. These results suggested that this newly developed approach is a viable and easy way to optimize the fermentation conditions for algicidal microorganisms.

journal_name

Microb Cell Fact

journal_title

Microbial cell factories

authors

Cai G,Zheng W,Yang X,Zhang B,Zheng T

doi

10.1186/1475-2859-13-75

subject

Has Abstract

pub_date

2014-05-24 00:00:00

pages

75

issn

1475-2859

pii

1475-2859-13-75

journal_volume

13

pub_type

杂志文章

相关文献

Microbial Cell Factories文献大全
  • Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice.

    abstract：BACKGROUND:Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. METHODS:We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxy...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0378-2

    authors： Shigemori S,Watanabe T,Kudoh K,Ihara M,Nigar S,Yamamoto Y,Suda Y,Sato T,Kitazawa H,Shimosato T

    更新日期：2015-11-25 00:00:00

  • Engineering an efficient and tight D-amino acid-inducible gene expression system in Rhodosporidium/Rhodotorula species.

    abstract：BACKGROUND:Rhodosporidium and Rhodotorula are two genera of oleaginous red yeast with great potential for industrial biotechnology. To date, there is no effective method for inducible expression of proteins and RNAs in these hosts. RESULTS:We have developed a luciferase gene reporter assay based on a new codon-optimiz...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0357-7

    authors： Liu Y,Koh CMJ,Ngoh ST,Ji L

    更新日期：2015-10-26 00:00:00

  • Inducible intracellular membranes: molecular aspects and emerging applications.

    abstract：:Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of ce...

    journal_title：Microbial cell factories

    pub_type： 杂志文章,评审

    doi：10.1186/s12934-020-01433-x

    authors： Royes J,Biou V,Dautin N,Tribet C,Miroux B

    更新日期：2020-09-04 00:00:00

  • Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract：BACKGROUND:The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO2 for biotechnological applications, the genetic toolbox for Synechocystis 6803 is still a limited factor. RESULTS:We systematical...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-018-0897-8

    authors： Liu D,Pakrasi HB

    更新日期：2018-03-26 00:00:00

  • The important ergot alkaloid intermediate chanoclavine-I produced in the yeast Saccharomyces cerevisiae by the combined action of EasC and EasE from Aspergillus japonicus.

    abstract：BACKGROUND:Ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. These compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. Bio...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0095-2

    authors： Nielsen CA,Folly C,Hatsch A,Molt A,Schröder H,O'Connor SE,Naesby M

    更新日期：2014-08-12 00:00:00

  • Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children's feces.

    abstract：BACKGROUND:Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hos...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1158-1

    authors： Jin H,Jeong Y,Yoo SH,Johnston TV,Ku S,Ji GE

    更新日期：2019-06-13 00:00:00

  • Relative contributions of non-essential Sec pathway components and cell envelope-associated proteases to high-level enzyme secretion by Bacillus subtilis.

    abstract：BACKGROUND:Bacillus subtilis is an important industrial workhorse applied in the production of many different commercially relevant proteins, especially enzymes. Virtually all of these proteins are secreted via the general secretion (Sec) pathway. Studies from different laboratories have demonstrated essential or non-e...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01315-2

    authors： Neef J,Bongiorni C,Schmidt B,Goosens VJ,van Dijl JM

    更新日期：2020-02-28 00:00:00

  • A novel method to prepare L-arabinose from xylose mother liquor by yeast-mediated biopurification.

    abstract：BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-10-43

    authors： Cheng H,Wang H,Lv J,Jiang M,Lin S,Deng Z

    更新日期：2011-06-07 00:00:00

  • Increased production of ganoderic acids by overexpression of homologous farnesyl diphosphate synthase and kinetic modeling of ganoderic acid production in Ganoderma lucidum.

    abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1164-3

    authors： Fei Y,Li N,Zhang DH,Xu JW

    更新日期：2019-06-28 00:00:00

  • Production and characterization of two medium-chain-length polydroxyalkanoates by engineered strains of Yarrowia lipolytica.

    abstract：BACKGROUND:The oleaginous yeast Yarrowia lipolytica is an organism of choice for the tailored production of various compounds such as biofuels or biopolymers. When properly engineered, it is capable of producing medium-chain-length polyhydroxyalkanoate (mcl-PHA), a biobased and biodegradable polymer that can be used as...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1140-y

    authors： Rigouin C,Lajus S,Ocando C,Borsenberger V,Nicaud JM,Marty A,Avérous L,Bordes F

    更新日期：2019-05-31 00:00:00

  • Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression.

    abstract：BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-018-0999-3

    authors： Wons E,Koscielniak D,Szadkowska M,Sektas M

    更新日期：2018-09-21 00:00:00

  • A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris.

    abstract：BACKGROUND:Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. To date, strain specific parameters, which are needed to set up feeding profiles for fed batch cultivations, are determined by time-consuming continuous cultures or consecutive ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-10-14

    authors： Dietzsch C,Spadiut O,Herwig C

    更新日期：2011-03-03 00:00:00

  • Bioprocess control in microscale: scalable fermentations in disposable and user-friendly microfluidic systems.

    abstract：BACKGROUND:The efficiency of biotechnological production processes depends on selecting the best performing microbial strain and the optimal cultivation conditions. Thus, many experiments have to be conducted, which conflicts with the demand to speed up drug development processes. Consequently, there is a great need fo...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-9-86

    authors： Funke M,Buchenauer A,Mokwa W,Kluge S,Hein L,Müller C,Kensy F,Büchs J

    更新日期：2010-11-13 00:00:00

  • Yeast prions form infectious amyloid inclusion bodies in bacteria.

    abstract：BACKGROUND:Prions were first identified as infectious proteins associated with fatal brain diseases in mammals. However, fungal prions behave as epigenetic regulators that can alter a range of cellular processes. These proteins propagate as self-perpetuating amyloid aggregates being an example of structural inheritance...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-11-89

    authors： Espargaró A,Villar-Piqué A,Sabaté R,Ventura S

    更新日期：2012-06-25 00:00:00

  • Nanotechnology, bionanotechnology and microbial cell factories.

    abstract：:Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...

    journal_title：Microbial cell factories

    pub_type： 社论

    doi：10.1186/1475-2859-9-53

    authors： Villaverde A

    更新日期：2010-07-05 00:00:00

  • Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyase.

    abstract：BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-4-35

    authors： Papi RM,Chaitidou SA,Trikka FA,Kyriakidis DA

    更新日期：2005-12-14 00:00:00

  • Production of biologically active human interleukin-10 by Bifidobacterium bifidum BGN4.

    abstract：BACKGROUND:Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been reported to have various in vivo and in vitro bio functionalities (e.g., anti-a...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01505-y

    authors： Hong N,Ku S,Yuk K,Johnston TV,Ji GE,Park MS

    更新日期：2021-01-19 00:00:00

  • Metabolic engineering of microorganisms for the production of L-arginine and its derivatives.

    abstract：:L-arginine (ARG) is an important amino acid for both medicinal and industrial applications. For almost six decades, the research has been going on for its improved industrial level production using different microorganisms. While the initial approaches involved random mutagenesis for increased tolerance to ARG and con...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0166-4

    authors： Shin JH,Lee SY

    更新日期：2014-12-03 00:00:00

  • Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite.

    abstract：BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0381-7

    authors： Bongers M,Chrysanthopoulos PK,Behrendorff JB,Hodson MP,Vickers CE,Nielsen LK

    更新日期：2015-11-26 00:00:00

  • Systematic pathway engineering of Corynebacterium glutamicum S9114 for L-ornithine production.

    abstract：BACKGROUND:L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS:In this study, Corynebacterium gl...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0776-8

    authors： Zhang B,Yu M,Zhou Y,Li Y,Ye BC

    更新日期：2017-09-22 00:00:00

  • MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker's yeast in lean dough.

    abstract：BACKGROUND:In Saccharomyces cerevisiae, alpha-glucosidase (maltase) is a key enzyme in maltose metabolism. In addition, the overexpression of the alpha-glucosidase-encoding gene MAL62 has been shown to increase the freezing tolerance of yeast in lean dough. However, its cryoprotection mechanism is still not clear. RES...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01454-6

    authors： Sun X,Zhang J,Fan ZH,Xiao P,Li F,Liu HQ,Zhu WB

    更新日期：2020-10-19 00:00:00

  • Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans.

    abstract：BACKGROUND:Lipases (EC 3.1.1.3) catalyze the hydrolysis of triacyl glycerol to glycerol and are involved in the synthesis of both short chain and long chain acylglycerols. They are widely used industrially in various applications, such as baking, laundry detergents and as biocatalysts in alternative energy strategies. ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-11-72

    authors： Selvin J,Kennedy J,Lejon DP,Kiran GS,Dobson AD

    更新日期：2012-06-01 00:00:00

  • Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.

    abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-018-1032-6

    authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

    更新日期：2018-11-24 00:00:00

  • Efficient ammonium uptake and mobilization of vacuolar arginine by Saccharomyces cerevisiae wine strains during wine fermentation.

    abstract：BACKGROUND:Under N-limiting conditions, Saccharomyces cerevisiae strains display a substantial variability in their biomass yield from consumed nitrogen -in particular wine yeasts exhibit high growth abilities- that is correlated with their capacity to complete alcoholic fermentation, a trait of interest for fermented ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0109-0

    authors： Crépin L,Sanchez I,Nidelet T,Dequin S,Camarasa C

    更新日期：2014-08-19 00:00:00

  • Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield.

    abstract：BACKGROUND:The production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically eukaryotic protein production experiments have relied on varying expression construct cassettes such as promoters and tags, or cultur...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-8-10

    authors： Bonander N,Darby RA,Grgic L,Bora N,Wen J,Brogna S,Poyner DR,O'Neill MA,Bill RM

    更新日期：2009-01-29 00:00:00

  • Construction of Escherichia coli cell factories for crocin biosynthesis.

    abstract：BACKGROUND:Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing attention, but there were no reports of successful implementation. Escherichia coli has been engin...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1166-1

    authors： Wang W,He P,Zhao D,Ye L,Dai L,Zhang X,Sun Y,Zheng J,Bi C

    更新日期：2019-07-05 00:00:00

  • Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger.

    abstract：BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-13-11

    authors： van der Straat L,Vernooij M,Lammers M,van den Berg W,Schonewille T,Cordewener J,van der Meer I,Koops A,de Graaff LH

    更新日期：2014-01-17 00:00:00

  • A new-generation of Bacillus subtilis cell factory for further elevated scyllo-inositol production.

    abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inosi...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0682-0

    authors： Tanaka K,Natsume A,Ishikawa S,Takenaka S,Yoshida KI

    更新日期：2017-04-21 00:00:00

  • Expressing a cytosolic pyruvate dehydrogenase complex to increase free fatty acid production in Saccharomyces cerevisiae.

    abstract：BACKGROUND:Saccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase (PDH) complex ex...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01493-z

    authors： Zhang Y,Su M,Qin N,Nielsen J,Liu Z

    更新日期：2020-12-10 00:00:00

  • Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills.

    abstract：BACKGROUND:The application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0797-3

    authors： Raddadi N,Giacomucci L,Totaro G,Fava F

    更新日期：2017-11-02 00:00:00