Expressing a cytosolic pyruvate dehydrogenase complex to increase free fatty acid production in Saccharomyces cerevisiae.

abstract：BACKGROUND:Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. RESULTS:In this stu...

journal_title：Microbial cell factories

authors： Zhang H,Liu Q,Cao Y,Feng X,Zheng Y,Zou H,Liu H,Yang J,Xian M

更新日期：2014-02-10 00:00:00

abstract：BACKGROUND:Messenger RNA decay is an important mechanism for controlling gene expression in all organisms. The rate of the mRNA degradation directly affects the steady state concentration of mRNAs and therefore influences the protein synthesis. RNaseE has a key importance for the general mRNA decay in E. coli. While RN...

journal_title：Microbial cell factories

authors： Kemmer C,Neubauer P

更新日期：2006-12-12 00:00:00

abstract：BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

journal_title：Microbial cell factories

authors： Upadhyay V,Singh A,Jha D,Singh A,Panda AK

更新日期：2016-06-08 00:00:00

abstract：BACKGROUND:Extracts and products (roots and/or aerial parts) from Echinacea ssp. represent a profitable market sector for herbal medicines thanks to different functional features. Alkamides and polyacetylenes, phenols like caffeic acid and its derivatives, polysaccharides and glycoproteins are the main bioactive compou...

journal_title：Microbial cell factories

authors： Rizzello CG,Coda R,Macías DS,Pinto D,Marzani B,Filannino P,Giuliani G,Paradiso VM,Di Cagno R,Gobbetti M

更新日期：2013-05-04 00:00:00

abstract：BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...

journal_title：Microbial cell factories

authors： Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

更新日期：2012-11-04 00:00:00

abstract：BACKGROUND:Thymosin α1 (Tα1), a 28-amino acid Nα-acetylated peptide, has a powerful general immunostimulating activity. Although biosynthesis is an attractive means of large-scale manufacture, to date, Tα1 can only be chemosynthesized because of two obstacles to its biosynthesis: the difficulties in expressing small pe...

journal_title：Microbial cell factories

authors： Ren Y,Yao X,Dai H,Li S,Fang H,Chen H,Zhou C

更新日期：2011-04-22 00:00:00

abstract：BACKGROUND:Chitinases are ubiquitous enzymes that have gained a recent biotechnological attention due to their ability to transform biological waste from chitin into valued chito-oligomers with wide agricultural, industrial or medical applications. The biological activity of these molecules is related to their size and...

journal_title：Microbial cell factories

authors： Kidibule PE,Santos-Moriano P,Jiménez-Ortega E,Ramírez-Escudero M,Limón MC,Remacha M,Plou FJ,Sanz-Aparicio J,Fernández-Lobato M

更新日期：2018-03-22 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, ...

journal_title：Microbial cell factories

authors： Yamaoka M,Osawa S,Morinaga T,Takenaka S,Yoshida K

更新日期：2011-09-07 00:00:00

abstract：BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...

journal_title：Microbial cell factories

authors： Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

更新日期：2015-04-17 00:00:00

abstract：BACKGROUND:Spore-forming bacteria of the Bacillus genus are widely used probiotics known to exert their beneficial effects also through the stimulation of the host immune response. The oral delivery of B. toyonensis spores has been shown to improve the immune response to a parenterally administered viral antigen in mic...

journal_title：Microbial cell factories

authors： Santos FDS,Mazzoli A,Maia AR,Saggese A,Isticato R,Leite F,Iossa S,Ricca E,Baccigalupi L

更新日期：2020-02-19 00:00:00

abstract：BACKGROUND:A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered ...

journal_title：Microbial cell factories

authors： Liu JL,Shriver-Lake LC,Anderson GP,Zabetakis D,Goldman ER

更新日期：2017-12-12 00:00:00

abstract：BACKGROUND:Oxygen-evolving photoautotrophic organisms, like cyanobacteria, protect their photosynthetic machinery by a number of regulatory mechanisms, including alternative electron transfer pathways. Despite the importance in modulating the electron flux distribution between the photosystems, alternative electron tra...

journal_title：Microbial cell factories

authors： Thiel K,Patrikainen P,Nagy C,Fitzpatrick D,Pope N,Aro EM,Kallio P

更新日期：2019-11-05 00:00:00

abstract：BACKGROUND:The filamentous fungus T. reesei effectively degrades cellulose and is known to produce various cellulolytic enzymes such as beta-glucosidase, endoglucanase, and cellobiohydrolase. The expression levels of each cellulase are controlled simultaneously, and their ratios and synergetic effects are important for...

journal_title：Microbial cell factories

authors： Yamada R,Taniguchi N,Tanaka T,Ogino C,Fukuda H,Kondo A

更新日期：2010-05-14 00:00:00

abstract：BACKGROUND:Omega hydroxy fatty acids (ω-OHFAs) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. However, the terminal oxygenation of saturated or unsaturated fatty acids for the synthesis of ω-OHFAs is intricate ...

journal_title：Microbial cell factories

authors： Durairaj P,Malla S,Nadarajan SP,Lee PG,Jung E,Park HH,Kim BG,Yun H

更新日期：2015-04-02 00:00:00

abstract：BACKGROUND:Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed. To this end, a crucial process is translation initiation which takes place at the ribosome binding s...

journal_title：Microbial cell factories

authors： Volkenborn K,Kuschmierz L,Benz N,Lenz P,Knapp A,Jaeger KE

更新日期：2020-07-29 00:00:00

abstract：BACKGROUND:Recombinant protein expression in bacteria often leads to the formation of intracellular insoluble protein deposits, a major bottleneck for the production of soluble and active products. However, in recent years, these bacterial protein aggregates, commonly known as inclusion bodies (IBs), have been shown to...

journal_title：Microbial cell factories

authors： Gil-Garcia M,Navarro S,Ventura S

更新日期：2020-06-01 00:00:00

abstract：BACKGROUND:Despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. Shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. Thu...

journal_title：Microbial cell factories

authors： Flitsch D,Ladner T,Lukacs M,Büchs J

更新日期：2016-02-24 00:00:00

abstract：BACKGROUND:Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the maj...

journal_title：Microbial cell factories

authors： Yin H,Zhang R,Xia M,Bai X,Mou J,Zheng Y,Wang M

更新日期：2017-06-15 00:00:00

abstract：BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...

journal_title：Microbial cell factories

authors： Yang CH,Liu EJ,Chen YL,Ou-Yang FY,Li SY

更新日期：2016-08-02 00:00:00

abstract：:Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In t...

journal_title：Microbial cell factories

authors： Yan S,Wu G

更新日期：2017-07-19 00:00:00

abstract：BACKGROUND:Under aerobic conditions, acetic acid is the major byproduct produced by E. coli during the fermentation. And acetic acid is detrimental to cell growth as it destroys transmembrane pH gradients. Hence, how to reduce the production of acetic acid and how to utilize it as a feedstock are of intriguing interest...

journal_title：Microbial cell factories

authors： Yang J,Nie Q

更新日期：2016-05-05 00:00:00

abstract：BACKGROUND:A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS:Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. O...

journal_title：Microbial cell factories

authors： Faijes M,Mars AE,Smid EJ

更新日期：2007-08-20 00:00:00

abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

journal_title：Microbial cell factories

authors： Fei Y,Li N,Zhang DH,Xu JW

更新日期：2019-06-28 00:00:00

abstract：BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...

journal_title：Microbial cell factories

authors： Zhang H,Li Z,Pereira B,Stephanopoulos G

更新日期：2015-09-15 00:00:00

abstract：BACKGROUND:Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. RESULTS:In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-produci...

journal_title：Microbial cell factories

authors： Lu H,Liu X,Huang M,Xia J,Chu J,Zhuang Y,Zhang S,Noorman H

更新日期：2015-09-17 00:00:00

abstract：BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

journal_title：Microbial cell factories

authors： Menzella HG

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose....

journal_title：Microbial cell factories

authors： Ying H,Tao S,Wang J,Ma W,Chen K,Wang X,Ouyang P

更新日期：2017-03-27 00:00:00

abstract：BACKGROUND:Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry to develop new molecules using structure-based drug design studies. Specifically, membrane-bound catechol-O-methyltransferase (MBCOMT) is an...

journal_title：Microbial cell factories

authors： Pedro AQ,Martins LM,Dias JM,Bonifácio MJ,Queiroz JA,Passarinha LA

更新日期：2015-08-07 00:00:00

abstract：BACKGROUND:Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications deriv...

journal_title：Microbial cell factories

authors： Sun H,Zhao D,Xiong B,Zhang C,Bi C

更新日期：2016-08-24 00:00:00

abstract：BACKGROUND:Strains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination and modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced in the ...

journal_title：Microbial cell factories

authors： Ma L,Li Y,Chen X,Ding M,Wu Y,Yuan YJ

更新日期：2019-03-11 00:00:00