Bottleneck in secretion of α-amylase in Bacillus subtilis.

abstract：: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been th...

journal_title：Microbial cell factories

authors： Gasser B,Saloheimo M,Rinas U,Dragosits M,Rodríguez-Carmona E,Baumann K,Giuliani M,Parrilli E,Branduardi P,Lang C,Porro D,Ferrer P,Tutino ML,Mattanovich D,Villaverde A

更新日期：2008-04-04 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：BACKGROUND:Predictable control of gene expression is necessary for the rational design and optimization of cell factories. In the yeast Saccharomyces cerevisiae, the promoter is one of the most important tools available for controlling gene expression. However, the complex expression patterns of yeast promoters have no...

journal_title：Microbial cell factories

authors： Peng B,Williams TC,Henry M,Nielsen LK,Vickers CE

更新日期：2015-06-26 00:00:00

abstract：BACKGROUND:Emergence of antibiotic resistance and growing consumer trend towards foods containing biopreservatives stimulated the search for alternative antimicrobials. This research is aimed at characterizing, investigating the mechanism of action, scale up optimization and evaluating the biopreservative potential of ...

journal_title：Microbial cell factories

authors： Wayah SB,Philip K

更新日期：2018-08-13 00:00:00

abstract：BACKGROUND:Intracellular metabolism of glucocorticoid hormones plays an important role in the pathogenesis of metabolic syndrome and regulates, among many physiological processes, collagen metabolism in skin. At the peripheral level the concentration of active glucocorticoids is mainly regulated by the 11β-hydroxystero...

journal_title：Microbial cell factories

authors： Vanella R,Callari R,Weston A,Heider H,Schwab MS,Kübler E

更新日期：2016-03-15 00:00:00

abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

journal_title：Microbial cell factories

authors： Fei Y,Li N,Zhang DH,Xu JW

更新日期：2019-06-28 00:00:00

abstract：BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...

journal_title：Microbial cell factories

authors： van der Straat L,Vernooij M,Lammers M,van den Berg W,Schonewille T,Cordewener J,van der Meer I,Koops A,de Graaff LH

更新日期：2014-01-17 00:00:00

abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...

journal_title：Microbial cell factories

authors： Wu W,Xing L,Zhou B,Lin Z

更新日期：2011-02-15 00:00:00

abstract：BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...

journal_title：Microbial cell factories

authors： Otto M,Teixeira PG,Vizcaino MI,David F,Siewers V

更新日期：2019-11-25 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...

journal_title：Microbial cell factories

authors： Rajamanickam V,Metzger K,Schmid C,Spadiut O

更新日期：2017-09-13 00:00:00

abstract：BACKGROUND:p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmf...

journal_title：Microbial cell factories

authors： Kawai Y,Noda S,Ogino C,Takeshima Y,Okai N,Tanaka T,Kondo A

更新日期：2013-05-07 00:00:00

abstract：BACKGROUND:Activation of silent biosynthetic gene clusters (BGCs) in marine-derived actinomycete strains is a feasible strategy to discover bioactive natural products. Actinoalloteichus sp. AHMU CJ021, isolated from the seashore, was shown to contain an intact but silent caerulomycin A (CRM A) BGC-cam in its genome. Th...

journal_title：Microbial cell factories

authors： Xie Y,Chen J,Wang B,Chen T,Chen J,Zhang Y,Liu X,Chen Q

更新日期：2020-08-06 00:00:00

abstract：BACKGROUND:The production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically eukaryotic protein production experiments have relied on varying expression construct cassettes such as promoters and tags, or cultur...

journal_title：Microbial cell factories

authors： Bonander N,Darby RA,Grgic L,Bora N,Wen J,Brogna S,Poyner DR,O'Neill MA,Bill RM

更新日期：2009-01-29 00:00:00

abstract：BACKGROUND:Gene therapy has gained an increasing interest in its anti-tumor efficiency. However, numerous efforts are required to promote them to clinics. In this study, a novel and efficient delivery platform based on bacterial magnetosomes (BMs) were developed, and the efficiency of BMs in delivering small interferin...

journal_title：Microbial cell factories

authors： Dai Q,Long R,Wang S,Kankala RK,Wang J,Jiang W,Liu Y

更新日期：2017-11-28 00:00:00

abstract：BACKGROUND:The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics and a work-intense s...

journal_title：Microbial cell factories

authors： Schwarzhans JP,Wibberg D,Winkler A,Luttermann T,Kalinowski J,Friehs K

更新日期：2016-05-20 00:00:00

abstract：BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...

journal_title：Microbial cell factories

authors： Pyeon HR,Nah HJ,Kang SH,Choi SS,Kim ES

更新日期：2017-05-31 00:00:00

abstract：BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...

journal_title：Microbial cell factories

authors： Li P,Sun H,Chen Z,Li Y,Zhu T

更新日期：2015-02-21 00:00:00

abstract：BACKGROUND:Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry to develop new molecules using structure-based drug design studies. Specifically, membrane-bound catechol-O-methyltransferase (MBCOMT) is an...

journal_title：Microbial cell factories

authors： Pedro AQ,Martins LM,Dias JM,Bonifácio MJ,Queiroz JA,Passarinha LA

更新日期：2015-08-07 00:00:00

abstract：BACKGROUND:The metabolic capabilities of acetogens to ferment a wide range of sugars, to grow autotrophically on H2/CO2, and more importantly on synthesis gas (H2/CO/CO2) make them very attractive candidates as production hosts for biofuels and biocommodities. Acetogenic metabolism is considered one of the earliest mod...

journal_title：Microbial cell factories

authors： Nagarajan H,Sahin M,Nogales J,Latif H,Lovley DR,Ebrahim A,Zengler K

更新日期：2013-11-25 00:00:00

abstract：BACKGROUND:In Saccharomyces cerevisiae, alpha-glucosidase (maltase) is a key enzyme in maltose metabolism. In addition, the overexpression of the alpha-glucosidase-encoding gene MAL62 has been shown to increase the freezing tolerance of yeast in lean dough. However, its cryoprotection mechanism is still not clear. RES...

journal_title：Microbial cell factories

authors： Sun X,Zhang J,Fan ZH,Xiao P,Li F,Liu HQ,Zhu WB

更新日期：2020-10-19 00:00:00

abstract：BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...

journal_title：Microbial cell factories

authors： Hossain AH,Li A,Brickwedde A,Wilms L,Caspers M,Overkamp K,Punt PJ

更新日期：2016-07-28 00:00:00

abstract：BACKGROUND:Most bacteria can use various compounds as carbon sources. These carbon sources can be either co-metabolized or sequentially metabolized, where the latter phenomenon typically occurs as catabolite repression. From the practical application point of view of utilizing lignocellulose for the production of biofu...

journal_title：Microbial cell factories

authors： Yao R,Hirose Y,Sarkar D,Nakahigashi K,Ye Q,Shimizu K

更新日期：2011-08-11 00:00:00

abstract：BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...

journal_title：Microbial cell factories

authors： Zhang H,Li Z,Pereira B,Stephanopoulos G

更新日期：2015-09-15 00:00:00

abstract：BACKGROUND:Green microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entiti...

journal_title：Microbial cell factories

authors： Sivakumar G,Jeong K,Lay JO Jr

更新日期：2014-06-03 00:00:00

abstract：:The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community easy and versatile access to the sequence, two web-sites have been installed as a resource for genomic sequence, gene and protein...

journal_title：Microbial cell factories

authors： Mattanovich D,Callewaert N,Rouzé P,Lin YC,Graf A,Redl A,Tiels P,Gasser B,De Schutter K

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-thr...

journal_title：Microbial cell factories

authors： Navarro D,Couturier M,da Silva GG,Berrin JG,Rouau X,Asther M,Bignon C

更新日期：2010-07-16 00:00:00

abstract：BACKGROUND:Oil palm empty fruit bunch (EFB) is a lignocellulosic waste produced in palm oil industry. EFB mainly consists of cellulose, hemicellulose (mainly xylan) and lignin and has a great potential to be reused. Converting EFB to fermentable sugars and value-added chemicals is a much better choice than treating EFB...

journal_title：Microbial cell factories

authors： Li Q,Ng WT,Wu JC

更新日期：2014-11-11 00:00:00

abstract：BACKGROUND:D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. ...

journal_title：Microbial cell factories

authors： Feng X,Jiang L,Han X,Liu X,Zhao Z,Liu H,Xian M,Zhao G

更新日期：2017-11-21 00:00:00

abstract：BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...

journal_title：Microbial cell factories

authors： Wons E,Koscielniak D,Szadkowska M,Sektas M

更新日期：2018-09-21 00:00:00

abstract：:A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tiss...

journal_title：Microbial cell factories

authors： Villaverde A,García-Fruitós E,Rinas U,Seras-Franzoso J,Kosoy A,Corchero JL,Vazquez E

更新日期：2012-06-11 00:00:00