Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid.

abstract：BACKGROUND:(2S)-Pinocembrin is a chiral flavanone with versatile pharmacological and biological activities. Its health-promoting effects have spurred on research effects on the microbial production of (2S)-pinocembrin. However, an often-overlooked salient feature in the analysis of microbial (2S)-pinocembrin is its chi...

journal_title：Microbial cell factories

authors： Guo L,Chen X,Li LN,Tang W,Pan YT,Kong JQ

更新日期：2016-02-04 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：BACKGROUND:Bacteriocin-producing Lactic acid bacteria (LAB) have vast applications in human and animal health, as well as in food industry. The structural, immunity, regulatory, export and modification genes are required for effective bacteriocin biosynthesis. Variations in gene sequence, composition and organisation w...

journal_title：Microbial cell factories

authors： Tai HF,Foo HL,Abdul Rahim R,Loh TC,Abdullah MP,Yoshinobu K

更新日期：2015-06-16 00:00:00

abstract：BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

journal_title：Microbial cell factories

authors： Menzella HG

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...

journal_title：Microbial cell factories

authors： Wu W,Xing L,Zhou B,Lin Z

更新日期：2011-02-15 00:00:00

abstract：BACKGROUND:Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol production. Although Z. mobilis is robustly resilient to certain abiotic stress such as ethanol, ...

journal_title：Microbial cell factories

authors： Fuchino K,Bruheim P

更新日期：2020-07-20 00:00:00

abstract：BACKGROUND:The biocontrol strain Pseudomonas chlororaphis GP72 isolated from the green pepper rhizosphere synthesizes three antifungal phenazine compounds, 2-Hydroxyphenazine (2-OH-PHZ), 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) and phenazine-1-carboxylic acid (PCA). PCA has been a commercialized antifungal pest...

journal_title：Microbial cell factories

authors： Liu K,Hu H,Wang W,Zhang X

更新日期：2016-07-28 00:00:00

abstract：BACKGROUND:Numerous challenges remain to achieve industrially competitive space-time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development and biocatalyst selection; studies at conventional lab scale are ti...

journal_title：Microbial cell factories

authors： Kolmar JF,Thum O,Baganz F

更新日期：2017-10-10 00:00:00

abstract：BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...

journal_title：Microbial cell factories

authors： Wu S,Gu W,Huang A,Li Y,Kumar M,Lim PE,Huan L,Gao S,Wang G

更新日期：2019-09-23 00:00:00

abstract：:Like natural viruses, manmade protein cages for drug delivery are to be ideally formed by repetitive subunits with self-assembling properties, mimicking viral functions and molecular organization. Naturally formed nanostructures (such as viruses, flagella or simpler protein oligomers) can be engineered to acquire spec...

journal_title：Microbial cell factories

authors： Vázquez E,Villaverde A

更新日期：2010-12-30 00:00:00

abstract：BACKGROUND:Streptomyces venezuelae ATCC 10712 produces antibiotics chloramphenicol (Cml) and jadomycin (Jad) in response to nutrient limitation and ethanol shock (ES), respectively. Biosynthesis of Cml and Jad was shown to be reciprocally regulated via the action of regulatory proteins JadR1 and JadR2 encoded by the ja...

journal_title：Microbial cell factories

authors： Sekurova ON,Zhang J,Kristiansen KA,Zotchev SB

更新日期：2016-05-20 00:00:00

abstract：:Innovative new genome engineering technologies for manipulating chromosomes have appeared in the last decade. One of these technologies, recombination mediated genetic engineering (recombineering) allows for precision DNA engineering of chromosomes and plasmids in Escherichia coli. Single-stranded DNA recombineering (...

journal_title：Microbial cell factories

authors： van Pijkeren JP,Britton RA

更新日期：2014-08-29 00:00:00

abstract：BACKGROUND:A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered ...

journal_title：Microbial cell factories

authors： Liu JL,Shriver-Lake LC,Anderson GP,Zabetakis D,Goldman ER

更新日期：2017-12-12 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...

journal_title：Microbial cell factories

authors： Rajamanickam V,Metzger K,Schmid C,Spadiut O

更新日期：2017-09-13 00:00:00

abstract：:Dunaliella salina is a unicellular green alga with a high α-linolenic acid (ALA) level, but a low eicosapentaenoic acid (EPA) level. In a previous analysis of the catalytic activity of delta 6 fatty acid desaturase (FADS6) from various species, FADS6 from Thalassiosira pseudonana (TpFADS6), a marine diatom, showed the...

journal_title：Microbial cell factories

authors： Shi H,Luo X,Wu R,Yue X

更新日期：2018-01-13 00:00:00

abstract：BACKGROUND:The rational design of L-phenylalanine (L-Phe) overproducing microorganisms has been successfully achieved by combining different genetic strategies such as inactivation of the phosphoenolpyruvate: phosphotransferase transport system (PTS) and overexpression of key genes (DAHP synthase, transketolase and cho...

journal_title：Microbial cell factories

authors： Báez-Viveros JL,Flores N,Juárez K,Castillo-España P,Bolivar F,Gosset G

更新日期：2007-09-19 00:00:00

abstract：BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...

journal_title：Microbial cell factories

authors： Pyeon HR,Nah HJ,Kang SH,Choi SS,Kim ES

更新日期：2017-05-31 00:00:00

abstract：BACKGROUND:Gene therapy has gained an increasing interest in its anti-tumor efficiency. However, numerous efforts are required to promote them to clinics. In this study, a novel and efficient delivery platform based on bacterial magnetosomes (BMs) were developed, and the efficiency of BMs in delivering small interferin...

journal_title：Microbial cell factories

authors： Dai Q,Long R,Wang S,Kankala RK,Wang J,Jiang W,Liu Y

更新日期：2017-11-28 00:00:00

abstract：BACKGROUND:Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of medical interest, as it is "Generally Recognized As Safe". Furthermore, as L. lactis naturally secretes only one major endoge...

journal_title：Microbial cell factories

authors： Samazan F,Rokbi B,Seguin D,Telles F,Gautier V,Richarme G,Chevret D,Varela PF,Velours C,Poquet I

更新日期：2015-07-16 00:00:00

abstract：BACKGROUND:Chinese Nong-flavor (NF) liquor is continuously and stably produced by solid-state fermentation technology for 1000 years, resulting in enrichment of special microbial community and enzymes system in its starter. Based on traditional culture-dependent methods, these functional enzymes are hardly obtained. Ac...

journal_title：Microbial cell factories

authors： Yi Z,Fang Y,He K,Liu D,Luo H,Zhao D,He H,Jin Y,Zhao H

更新日期：2018-02-22 00:00:00

abstract：BACKGROUND:Calreticulin (CRT) resides in the endoplasmic reticulum (ER) and functions to chaperone proteins, ensuring proper folding, and intracellular Ca(2+) homeostasis. Emerging evidence shows that CRT is a multifunctional protein with significant roles in physiological and pathological processes with presence both ...

journal_title：Microbial cell factories

authors： Čiplys E,Žitkus E,Gold LI,Daubriac J,Pavlides SC,Højrup P,Houen G,Wang WA,Michalak M,Slibinskas R

更新日期：2015-10-15 00:00:00

abstract：:Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In t...

journal_title：Microbial cell factories

authors： Yan S,Wu G

更新日期：2017-07-19 00:00:00

abstract：BACKGROUND:Despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. Shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. Thu...

journal_title：Microbial cell factories

authors： Flitsch D,Ladner T,Lukacs M,Büchs J

更新日期：2016-02-24 00:00:00

abstract：BACKGROUND:Phasin (PhaP), a kind of polyhydroxyalkanoate (PHA) granule-associated proteins, has a role in controlling the properties of PHA granules surface, and is thought to have influence on PHA biosynthesis in PHA-producing bacteria. This study focused on the phaP1(Re) locus in Ralstonia eutropha as a site of chrom...

journal_title：Microbial cell factories

authors： Kawashima Y,Orita I,Nakamura S,Fukui T

更新日期：2015-11-23 00:00:00

abstract：BACKGROUND:Recent years have seen a huge growth in the market of industrial yeasts with the need for strains affording better performance or to be used in new applications. Stress tolerance of commercial Saccharomyces cerevisiae yeasts is, without doubt, a trait that needs improving. Such trait is, however, complex, an...

journal_title：Microbial cell factories

authors： Pérez-Torrado R,Panadero J,Hernández-López MJ,Prieto JA,Randez-Gil F

更新日期：2010-07-13 00:00:00

abstract：BACKGROUND:The Bacillus subtilis spore has long been used to display antigens and enzymes. Spore display can be accomplished by a recombinant and a non-recombinant approach, with the latter proved more efficient than the recombinant one. We used the non-recombinant approach to independently adsorb two thermophilic enzy...

journal_title：Microbial cell factories

authors： Mattossovich R,Iacono R,Cangiano G,Cobucci-Ponzano B,Isticato R,Moracci M,Ricca E

更新日期：2017-11-28 00:00:00

abstract：BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...

journal_title：Microbial cell factories

authors： de la Torre A,Metivier A,Chu F,Laurens LM,Beck DA,Pienkos PT,Lidstrom ME,Kalyuzhnaya MG

更新日期：2015-11-25 00:00:00

abstract：: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been th...

journal_title：Microbial cell factories

authors： Gasser B,Saloheimo M,Rinas U,Dragosits M,Rodríguez-Carmona E,Baumann K,Giuliani M,Parrilli E,Branduardi P,Lang C,Porro D,Ferrer P,Tutino ML,Mattanovich D,Villaverde A

更新日期：2008-04-04 00:00:00

abstract：BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...

journal_title：Microbial cell factories

authors： Zhang F,Qian X,Si H,Xu G,Han R,Ni Y

更新日期：2015-11-05 00:00:00

abstract：BACKGROUND:The metabolic capabilities of acetogens to ferment a wide range of sugars, to grow autotrophically on H2/CO2, and more importantly on synthesis gas (H2/CO/CO2) make them very attractive candidates as production hosts for biofuels and biocommodities. Acetogenic metabolism is considered one of the earliest mod...

journal_title：Microbial cell factories

authors： Nagarajan H,Sahin M,Nogales J,Latif H,Lovley DR,Ebrahim A,Zengler K

更新日期：2013-11-25 00:00:00