Easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles.

abstract：BACKGROUND:Zygosaccharomyces bailii is a diploid budding yeast still poorly characterized, but widely recognised as tolerant to several stresses, most of which related to industrial processes of production. Because of that, it would be very interesting to develop its ability as a cell factory. Gas1p is a beta-1,3-gluca...

journal_title：Microbial cell factories

authors： Passolunghi S,Riboldi L,Dato L,Porro D,Branduardi P

更新日期：2010-01-26 00:00:00

abstract：BACKGROUND:Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity and efficacy, a variety of membrane receptors. Disulphide-reticulated peptides have evolved to display improved specificity, low immunogenicity and t...

journal_title：Microbial cell factories

authors： Turchetto J,Sequeira AF,Ramond L,Peysson F,Brás JL,Saez NJ,Duhoo Y,Blémont M,Guerreiro CI,Quinton L,De Pauw E,Gilles N,Darbon H,Fontes CM,Vincentelli R

更新日期：2017-01-17 00:00:00

abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

journal_title：Microbial cell factories

authors： Fei Y,Li N,Zhang DH,Xu JW

更新日期：2019-06-28 00:00:00

abstract：BACKGROUND:Targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the N-terminus of a secretory protein. These signal sequences usually contain an N-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signa...

journal_title：Microbial cell factories

authors： Yarimizu T,Nakamura M,Hoshida H,Akada R

更新日期：2015-02-14 00:00:00

abstract：:Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of ce...

journal_title：Microbial cell factories

authors： Royes J,Biou V,Dautin N,Tribet C,Miroux B

更新日期：2020-09-04 00:00:00

abstract：BACKGROUND:Bacterial surface display is of interest in many applications, including live vaccine development, screening of protein libraries and the development of whole cell biocatalysts. The goal of this work was to understand which parameters result in production of large quantities of cells that at the same time ex...

journal_title：Microbial cell factories

authors： Gustavsson M,Bäcklund E,Larsson G

更新日期：2011-09-14 00:00:00

abstract：BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...

journal_title：Microbial cell factories

authors： de la Torre A,Metivier A,Chu F,Laurens LM,Beck DA,Pienkos PT,Lidstrom ME,Kalyuzhnaya MG

更新日期：2015-11-25 00:00:00

abstract：BACKGROUND:The biocontrol strain Pseudomonas chlororaphis GP72 isolated from the green pepper rhizosphere synthesizes three antifungal phenazine compounds, 2-Hydroxyphenazine (2-OH-PHZ), 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) and phenazine-1-carboxylic acid (PCA). PCA has been a commercialized antifungal pest...

journal_title：Microbial cell factories

authors： Liu K,Hu H,Wang W,Zhang X

更新日期：2016-07-28 00:00:00

abstract：BACKGROUND:Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the mitochondrial thiol oxidase ERV1 gene was the most effective for high GSSG production in Saccharomyc...

journal_title：Microbial cell factories

authors： Kobayashi J,Sasaki D,Hara KY,Hasunuma T,Kondo A

更新日期：2017-03-15 00:00:00

abstract：BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...

journal_title：Microbial cell factories

authors： Qiu C,Chen X,Rexida R,Shen Y,Qi Q,Bao X,Hou J

更新日期：2020-07-20 00:00:00

abstract：BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...

journal_title：Microbial cell factories

authors： Baritugo KA,Kim HT,David Y,Khang TU,Hyun SM,Kang KH,Yu JH,Choi JH,Song JJ,Joo JC,Park SJ

更新日期：2018-08-21 00:00:00

abstract：BACKGROUND:Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages, which is why current ...

journal_title：Microbial cell factories

authors： Capone S,Horvat J,Herwig C,Spadiut O

更新日期：2015-07-10 00:00:00

abstract：BACKGROUND:Proteins can be secreted from a host organism with the aid of N-terminal secretion signals. The budding yeast Pichia pastoris (Komagataella sp.) is widely employed to secrete proteins of academic and industrial interest. For this yeast, the most commonly used secretion signal is the N-terminal portion of pre...

journal_title：Microbial cell factories

authors： Barrero JJ,Casler JC,Valero F,Ferrer P,Glick BS

更新日期：2018-10-12 00:00:00

abstract：BACKGROUND:Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose r...

journal_title：Microbial cell factories

authors： Cheng H,Wang S,Bilal M,Ge X,Zhang C,Fickers P,Cheng H

更新日期：2018-08-29 00:00:00

abstract：BACKGROUND:Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol production. Although Z. mobilis is robustly resilient to certain abiotic stress such as ethanol, ...

journal_title：Microbial cell factories

authors： Fuchino K,Bruheim P

更新日期：2020-07-20 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, ...

journal_title：Microbial cell factories

authors： Yamaoka M,Osawa S,Morinaga T,Takenaka S,Yoshida K

更新日期：2011-09-07 00:00:00

abstract：BACKGROUND:The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO2 for biotechnological applications, the genetic toolbox for Synechocystis 6803 is still a limited factor. RESULTS:We systematical...

journal_title：Microbial cell factories

authors： Liu D,Pakrasi HB

更新日期：2018-03-26 00:00:00

abstract：BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...

journal_title：Microbial cell factories

authors： Pyeon HR,Nah HJ,Kang SH,Choi SS,Kim ES

更新日期：2017-05-31 00:00:00

abstract：BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...

journal_title：Microbial cell factories

authors： Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

更新日期：2012-11-04 00:00:00

abstract：BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...

journal_title：Microbial cell factories

authors： Bruschi M,Krömer JO,Steen JA,Nielsen LK

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:Protoilludene is a valuable sesquiterpene and serves as a precursor for several medicinal compounds and antimicrobial chemicals. It can be synthesized by heterologous expression of protoilludene synthase in Escherichia coli with overexpression of mevalonate (MVA) or methylerythritol-phosphate (MEP) pathway, ...

journal_title：Microbial cell factories

authors： Yang L,Wang C,Zhou J,Kim SW

更新日期：2016-01-19 00:00:00

abstract：BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...

journal_title：Microbial cell factories

authors： Otto M,Teixeira PG,Vizcaino MI,David F,Siewers V

更新日期：2019-11-25 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：BACKGROUND:Bacterial biofilms are a preferred mode of growth for many types of microorganisms in their natural environments. The ability of pathogens to integrate within a biofilm is pivotal to their survival. The possibility of biofilm formation in Lactobacillus communities is also important in various industrial and ...

journal_title：Microbial cell factories

authors： Jalilsood T,Baradaran A,Song AA,Foo HL,Mustafa S,Saad WZ,Yusoff K,Rahim RA

更新日期：2015-07-07 00:00:00

abstract：BACKGROUND:A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in order to maintain adequate life-long immune memory: this demands for high production levels and degre...

journal_title：Microbial cell factories

authors： Piubelli L,Campa M,Temporini C,Binda E,Mangione F,Amicosante M,Terreni M,Marinelli F,Pollegioni L

更新日期：2013-11-19 00:00:00

abstract：BACKGROUND:Plasmid expression is a popular method in studies of MVA pathway for isoprenoid production in Escherichia coli. However, heterologous gene expression with plasmid is often not stable and might burden growth of host cells, decreases cell mass and product yield. In this study, MVA pathway was divided into thre...

journal_title：Microbial cell factories

authors： Ye L,Zhang C,Bi C,Li Q,Zhang X

更新日期：2016-12-01 00:00:00

abstract：BACKGROUND:Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest...

journal_title：Microbial cell factories

authors： Vogt CM,Schraner EM,Aguilar C,Eichwald C

更新日期：2016-08-11 00:00:00

abstract：BACKGROUND:The expression of heterologous proteins in Escherichia coli is strongly affected by codon bias. This phenomenon occurs when the codon usage of the mRNA coding for the foreign protein differs from that of the bacterium. The ribosome pauses upon encountering a rare codon and may detach from the mRNA, thereby t...

journal_title：Microbial cell factories

authors： Rosano GL,Ceccarelli EA

更新日期：2009-07-24 00:00:00

abstract：:Recent evidence has demonstrated that cell-to-cell signaling is a fundamental activity carried out by numerous microorganisms. A number of specialized processes are reported to be regulated by density-dependent signaling molecules including antibiotic production, bioluminescence, biofilm formation, genetic competence,...

journal_title：Microbial cell factories

authors： DeLisa MP,Bentley WE

更新日期：2002-12-20 00:00:00

abstract：BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...

journal_title：Microbial cell factories

authors： Scalcinati G,Partow S,Siewers V,Schalk M,Daviet L,Nielsen J

更新日期：2012-08-31 00:00:00