Abstract:
:Dunaliella salina is a unicellular green alga with a high α-linolenic acid (ALA) level, but a low eicosapentaenoic acid (EPA) level. In a previous analysis of the catalytic activity of delta 6 fatty acid desaturase (FADS6) from various species, FADS6 from Thalassiosira pseudonana (TpFADS6), a marine diatom, showed the highest catalytic activity for ALA. In this study, to enhance EPA production in D. salina, FADS6 from D. salina (DsFADS6) was identified, and substrate specificities for DsFADS6 and TpFADS6 were characterized. Furthermore, a plasmid harboring the TpFADS6 gene was constructed and overexpressed in D. salina. Our results revealed that EPA production reached 21.3 ± 1.5 mg/L in D. salina transformants. To further increase EPA production, myoinositol (MI) was used as a growth-promoting agent; it increased the dry cell weight of D. salina transformants, and EPA production reached 91.3 ± 11.6 mg/L. The combination of 12% CO2 aeration with glucose/KNO3 in the medium improved EPA production to 192.9 ± 25.7 mg/L in the Ds-TpFADS6 transformant. We confirmed that the increase in ALA was optimal at 8 °C; the EPA percentage reached 41.12 ± 4.78%. The EPA yield was further increased to 554.3 ± 95.6 mg/L by supplementation with 4 g/L perilla seed meal (PeSM), 500 mg/L MI, and 12% CO2 aeration with glucose/KNO3 at varying temperatures. EPA production and the percentage of EPA in D. salina were 343.8-fold and 25-fold higher than those in wild-type D. salina, respectively. IMPORTANCE:FADS6 from Thalassiosira pseudonana, which demonstrates high catalytic activity toward α-linolenic acid, was used to enhance EPA production by Dunaliella salina. Transformation of FADS6 from Thalassiosira pseudonana into Dunaliella salina with myoinositol, CO2, low temperatures, and perilla seed meal supplementation substantially increased EPA production in Dunaliella salina to 554.3 ± 95.6 mg/L. Accordingly, D. salina could be a potential alternative source of EPA and is suitable for its large-scale production.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Shi H,Luo X,Wu R,Yue Xdoi
10.1186/s12934-018-0857-3subject
Has Abstractpub_date
2018-01-13 00:00:00pages
7issue
1issn
1475-2859pii
10.1186/s12934-018-0857-3journal_volume
17pub_type
杂志文章abstract:: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been th...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-7-11
更新日期:2008-04-04 00:00:00
abstract:BACKGROUND:Chitinases are ubiquitous enzymes that have gained a recent biotechnological attention due to their ability to transform biological waste from chitin into valued chito-oligomers with wide agricultural, industrial or medical applications. The biological activity of these molecules is related to their size and...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0895-x
更新日期:2018-03-22 00:00:00
abstract:BACKGROUND:A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in order to maintain adequate life-long immune memory: this demands for high production levels and degre...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-115
更新日期:2013-11-19 00:00:00
abstract:BACKGROUND:Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermophilus α-amylase in B...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1119-8
更新日期:2019-04-11 00:00:00
abstract::The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/s12934-020-1289-4
更新日期:2020-02-05 00:00:00
abstract:BACKGROUND:Ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. These compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. Bio...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0095-2
更新日期:2014-08-12 00:00:00
abstract:BACKGROUND:Numerous challenges remain to achieve industrially competitive space-time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development and biocatalyst selection; studies at conventional lab scale are ti...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0788-4
更新日期:2017-10-10 00:00:00
abstract:BACKGROUND:Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the maj...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0717-6
更新日期:2017-06-15 00:00:00
abstract:BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-145
更新日期:2012-11-04 00:00:00
abstract:BACKGROUND:The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have problems such as screeni...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-30
更新日期:2010-05-11 00:00:00
abstract:BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-4-35
更新日期:2005-12-14 00:00:00
abstract:BACKGROUND:Recent years have seen a huge growth in the market of industrial yeasts with the need for strains affording better performance or to be used in new applications. Stress tolerance of commercial Saccharomyces cerevisiae yeasts is, without doubt, a trait that needs improving. Such trait is, however, complex, an...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-56
更新日期:2010-07-13 00:00:00
abstract::Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...
journal_title:Microbial cell factories
pub_type: 社论
doi:10.1186/1475-2859-9-53
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0936-5
更新日期:2018-06-07 00:00:00
abstract:BACKGROUND:Under aerobic conditions, acetic acid is the major byproduct produced by E. coli during the fermentation. And acetic acid is detrimental to cell growth as it destroys transmembrane pH gradients. Hence, how to reduce the production of acetic acid and how to utilize it as a feedstock are of intriguing interest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0475-x
更新日期:2016-05-05 00:00:00
abstract:BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1213-y
更新日期:2019-10-10 00:00:00
abstract:BACKGROUND:Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-47
更新日期:2010-06-17 00:00:00
abstract:BACKGROUND:In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Alt...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0538-z
更新日期:2016-08-11 00:00:00
abstract:BACKGROUND:Bacillus subtilis is an important industrial workhorse applied in the production of many different commercially relevant proteins, especially enzymes. Virtually all of these proteins are secreted via the general secretion (Sec) pathway. Studies from different laboratories have demonstrated essential or non-e...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01315-2
更新日期:2020-02-28 00:00:00
abstract:BACKGROUND:Griseoviridin (GV) and viridogrisein (VG, also referred as etamycin), both biosynthesized by a distinct 105 kb biosynthetic gene cluster (BGC) in Streptomyces griseoviridis NRRL 2427, are a pair of synergistic streptogramin antibiotics and very important in treating infections of many multi-drug resistant mi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0792-8
更新日期:2017-10-25 00:00:00
abstract:BACKGROUND:To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-thr...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-58
更新日期:2010-07-16 00:00:00
abstract:BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0381-7
更新日期:2015-11-26 00:00:00
abstract:BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1113-1
更新日期:2019-03-28 00:00:00
abstract:BACKGROUND:Trypsin has many applications in food and pharmaceutical manufacturing. Although commercial trypsin is usually extracted from porcine pancreas, this source carries the risks of infectivity and immunogenicity. Microbial Streptomyces griseus trypsin (SGT) is a prime alternative because it possesses efficient h...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01338-9
更新日期:2020-04-13 00:00:00
abstract:BACKGROUND:D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0822-6
更新日期:2017-11-21 00:00:00
abstract:BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0206-8
更新日期:2015-02-21 00:00:00
abstract:BACKGROUND:Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose r...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0982-z
更新日期:2018-08-29 00:00:00
abstract:BACKGROUND:Terpenoids constitute a large family of natural products, attracting commercial interest for a variety of uses as flavours, fragrances, drugs and alternative fuels. Saccharomyces cerevisiae offers a versatile cell factory, as the precursors of terpenoid biosynthesis are naturally synthesized by the sterol bi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-4
更新日期:2011-01-28 00:00:00
abstract:BACKGROUND:Bioethanol produced by the yeast Saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. Lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. However, hemicellulosic materials cannot be...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0145-9
更新日期:2014-10-12 00:00:00
abstract:BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0708-7
更新日期:2017-05-31 00:00:00