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Production of human blood group B antigen epitope conjugated protein in Escherichia coli and utilization of the adsorption blood group B antibody.

Abstract：

BACKGROUND:In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Although there are commercial columns to do this work, the application is still limited because of the high production cost. RESULTS:In this study, the PglB glycosylation pathway from Campylobacter jejuni was exploited to produce glycoprotein conjugated with Escherichia coli O86:B7 O-antigen, which bears the blood group B antigen epitope to absorb blood group B antibody in blood. The titers of blood group B antibody were reduced to a safe level without changing the clotting function of plasma after glycoprotein absorption of B antibodies in the plasma. CONCLUSIONS:We developed a feasible strategy for the specific adsorption/removal of blood group antibodies. This method will be useful in ABOi organ transplantation and universal blood transfusion.

journal_name

Microb Cell Fact

journal_title

Microbial cell factories

authors

Shang W,Zhai Y,Ma Z,Yang G,Ding Y,Han D,Li J,Zhang H,Liu J,Wang PG,Liu XW,Chen M

doi

10.1186/s12934-016-0538-z

subject

Has Abstract

pub_date

2016-08-11 00:00:00

pages

138

issue

1

issn

1475-2859

pii

10.1186/s12934-016-0538-z

journal_volume

15

pub_type

杂志文章

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