Abstract：
:Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fungal-derived BPP phytase. We identified a phytase highly differentially expressed only in the parasitic stage of a nematophagous fungus, Arhtrobotrys oliogospora, during the development of the 3D traps. We found that this phytase was homologous to the known bacterial BPP phytase, thus we referred the new phytase to Aophytase. The heterologous expression of codon-optimized Aophytase gene in Pichia pastoris was successfully investigated to yield recombinant Aophytase (r-Aophytase) with high specific enzyme activity of 74.71 U/mg, much higher than those of recombinant BPP phytases derived bacteria. The kinetic parameters of the r-Aophytase, the optimum pH and temperature, as well as the effects of surfactant, EDTA and different ions on its enzyme activity were further investigated. The potential utilization of r-Aophytase in feed processing was finally explored. We found that the optimal pH value was about 7.5, and the optimal temperature was 50 °C.; r-Aophytase significantly increased the release of inorganic phosphorus from soybean meal, and improved the release of soluble minerals from the durum wheat flour and finger millet flour. The findings indicate its potential utilization in the feed processing to ameliorate nutritional value of cereals and animal feed in the future.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Hou X,Shen Z,Li N,Kong X,Sheng K,Wang J,Wang Ydoi
10.1186/s12934-020-01346-9subject
Has Abstractpub_date
2020-04-06 00:00:00pages
84issue
1issn
1475-2859pii
10.1186/s12934-020-01346-9journal_volume
19pub_type
杂志文章abstract：BACKGROUND:Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their superior physical and mechanical properties. We engineered a reversed fatty acid β-oxidation pathway i...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-019-1186-x
更新日期：2019-08-13 00:00:00
abstract：BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01466-2
更新日期：2020-11-23 00:00:00
abstract：BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0243-3
更新日期：2015-04-17 00:00:00
abstract：BACKGROUND:The rational design of L-phenylalanine (L-Phe) overproducing microorganisms has been successfully achieved by combining different genetic strategies such as inactivation of the phosphoenolpyruvate: phosphotransferase transport system (PTS) and overexpression of key genes (DAHP synthase, transketolase and cho...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-6-30
更新日期：2007-09-19 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0529-0
更新日期：2016-07-28 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-5-38
更新日期：2006-12-12 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0788-4
更新日期：2017-10-10 00:00:00
abstract：BACKGROUND:Streptomyces venezuelae ATCC 10712 produces antibiotics chloramphenicol (Cml) and jadomycin (Jad) in response to nutrient limitation and ethanol shock (ES), respectively. Biosynthesis of Cml and Jad was shown to be reciprocally regulated via the action of regulatory proteins JadR1 and JadR2 encoded by the ja...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0484-9
更新日期：2016-05-20 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0532-5
更新日期：2016-08-11 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0356-8
更新日期：2015-10-15 00:00:00
abstract：BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-10-36
更新日期：2011-05-18 00:00:00
abstract：BACKGROUND:Bacillus subtilis has been widely used as a host for heterologous protein expression in food industry. B. subtilis ATCC6051 is an alternative expression host for the production of industrial enzymes, and exhibits favorable growth properties compared to B. subtilis 168. Extracellular expression of pullulanase...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-1011-y
更新日期：2018-10-22 00:00:00
abstract：:Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features...
journal_title：Microbial cell factories
pub_type： 杂志文章,评审
doi：10.1186/s12934-017-0669-x
更新日期：2017-04-04 00:00:00
abstract：BACKGROUND:Oxygen-evolving photoautotrophic organisms, like cyanobacteria, protect their photosynthetic machinery by a number of regulatory mechanisms, including alternative electron transfer pathways. Despite the importance in modulating the electron flux distribution between the photosystems, alternative electron tra...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-019-1238-2
更新日期：2019-11-05 00:00:00
abstract：BACKGROUND:In past years research has focused on the development of alternative Gram positive bacterial expression systems to produce industrially relevant proteins. Brevibacillus choshinensis is an easy to handle non-sporulating bacterium, lacking extracellular proteases, that has been already shown to provide a high ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-12-12
更新日期：2013-02-01 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0776-8
更新日期：2017-09-22 00:00:00
abstract：BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0999-3
更新日期：2018-09-21 00:00:00
abstract：BACKGROUND:Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0693-x
更新日期：2017-05-02 00:00:00
abstract：BACKGROUND:Despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. Shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. Thu...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0444-4
更新日期：2016-02-24 00:00:00
abstract：: Microbes utilize enzymes to perform a variety of functions. Enzymes are biocatalysts working as highly efficient machines at the molecular level. In the past, enzymes have been viewed as static entities and their function has been explained on the basis of direct structural interactions between the enzyme and the sub...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-5-2
更新日期：2006-01-12 00:00:00
abstract：BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0377-3
更新日期：2015-11-25 00:00:00
abstract：BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01458-2
更新日期：2020-11-02 00:00:00
abstract：BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0319-0
更新日期：2015-09-15 00:00:00
abstract：BACKGROUND:Glucose is the main carbon source of E. coli and a typical substrate in production processes. The main glucose uptake system is the glucose specific phosphotransferase system (Glc-PTS). The PTS couples glucose uptake with its phosphorylation. This is achieved by the concomitant conversion of phosphoenolpyruv...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0980-1
更新日期：2018-08-28 00:00:00
abstract：BACKGROUND:Prions were first identified as infectious proteins associated with fatal brain diseases in mammals. However, fungal prions behave as epigenetic regulators that can alter a range of cellular processes. These proteins propagate as self-perpetuating amyloid aggregates being an example of structural inheritance...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-89
更新日期：2012-06-25 00:00:00
abstract：BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0977-9
更新日期：2018-08-21 00:00:00
abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0768-8
更新日期：2017-09-13 00:00:00
abstract：INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-1036-2
更新日期：2018-12-03 00:00:00
abstract：BACKGROUND:A cellular stress response is triggered upon induction of recombinant protein expression which feedback inhibits both growth as well as protein synthesis. In order to separate these two effects, it was decided to study "quiescent cultures" which continue to be metabolically active and express recombinant pro...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0744-3
更新日期：2017-07-28 00:00:00
abstract：BACKGROUND:Gene therapy has gained an increasing interest in its anti-tumor efficiency. However, numerous efforts are required to promote them to clinics. In this study, a novel and efficient delivery platform based on bacterial magnetosomes (BMs) were developed, and the efficiency of BMs in delivering small interferin...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0830-6
更新日期：2017-11-28 00:00:00
