A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris.

abstract：BACKGROUND:Due to their high energy density and compatible physical properties, several monoterpenes have been investigated as potential renewable transportation fuels, either as blendstocks with petroleum or as drop-in replacements for use in vehicles (both heavy and light-weight) or in aviation. Sustainable microbial...

journal_title：Microbial cell factories

authors： Zhuang X,Kilian O,Monroe E,Ito M,Tran-Gymfi MB,Liu F,Davis RW,Mirsiaghi M,Sundstrom E,Pray T,Skerker JM,George A,Gladden JM

更新日期：2019-03-18 00:00:00

abstract：BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...

journal_title：Microbial cell factories

authors： Rohe P,Venkanna D,Kleine B,Freudl R,Oldiges M

更新日期：2012-10-31 00:00:00

abstract：:The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...

journal_title：Microbial cell factories

authors： Liu Q,Yu Z,Tian F,Zhao J,Zhang H,Zhai Q,Chen W

更新日期：2020-02-05 00:00:00

abstract：BACKGROUND:Yeast viability and vitality are essential for different industrial processes where the yeast Saccharomyces cerevisiae is used as a biotechnological tool. Therefore, the decline of yeast biological functions during aging may compromise their successful biotechnological use. Life span is controlled by a varie...

journal_title：Microbial cell factories

authors： Orozco H,Matallana E,Aranda A

更新日期：2013-01-02 00:00:00

abstract：BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...

journal_title：Microbial cell factories

authors： Lehtinen T,Efimova E,Santala S,Santala V

更新日期：2018-02-08 00:00:00

abstract：BACKGROUND:Terpenoids constitute a large family of natural products, attracting commercial interest for a variety of uses as flavours, fragrances, drugs and alternative fuels. Saccharomyces cerevisiae offers a versatile cell factory, as the precursors of terpenoid biosynthesis are naturally synthesized by the sterol bi...

journal_title：Microbial cell factories

authors： Ignea C,Cvetkovic I,Loupassaki S,Kefalas P,Johnson CB,Kampranis SC,Makris AM

更新日期：2011-01-28 00:00:00

abstract：BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...

journal_title：Microbial cell factories

authors： Yang CH,Liu EJ,Chen YL,Ou-Yang FY,Li SY

更新日期：2016-08-02 00:00:00

abstract：BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...

journal_title：Microbial cell factories

authors： Silva R,Aguiar TQ,Coelho E,Jiménez A,Revuelta JL,Domingues L

更新日期：2019-03-28 00:00:00

abstract：BACKGROUND:In past years research has focused on the development of alternative Gram positive bacterial expression systems to produce industrially relevant proteins. Brevibacillus choshinensis is an easy to handle non-sporulating bacterium, lacking extracellular proteases, that has been already shown to provide a high ...

journal_title：Microbial cell factories

authors： D'Urzo N,Martinelli M,Nenci C,Brettoni C,Telford JL,Maione D

更新日期：2013-02-01 00:00:00

abstract：BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

journal_title：Microbial cell factories

authors： Upadhyay V,Singh A,Jha D,Singh A,Panda AK

更新日期：2016-06-08 00:00:00

abstract：BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...

journal_title：Microbial cell factories

authors： Papi RM,Chaitidou SA,Trikka FA,Kyriakidis DA

更新日期：2005-12-14 00:00:00

abstract：BACKGROUND:Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, constituting direct repeats (D...

journal_title：Microbial cell factories

authors： Ding T,Huang C,Liang Z,Ma X,Wang N,Huo YX

更新日期：2020-03-10 00:00:00

abstract：BACKGROUND:L-tryptophan is an aromatic amino acid widely used in the food, chemical and pharmaceutical industries. In Escherichia coli, L-tryptophan is synthesized from phosphoenolpyruvate and erythrose 4-phosphate by enzymes in the shikimate pathway and L-tryptophan branch pathway, while L-serine and phosphoribosylpyr...

journal_title：Microbial cell factories

authors： Gu P,Yang F,Kang J,Wang Q,Qi Q

更新日期：2012-03-02 00:00:00

abstract：BACKGROUND:For the cultivation of Escherichia coli in bioreactors trace element solutions are generally designed for optimal growth under aerobic conditions. They do normally not contain selenium and nickel. Molybdenum is only contained in few of them. These elements are part of the formate hydrogen lyase (FHL) complex...

journal_title：Microbial cell factories

authors： Soini J,Ukkonen K,Neubauer P

更新日期：2008-08-07 00:00:00

abstract：BACKGROUND:Bioethanol produced by the yeast Saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. Lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. However, hemicellulosic materials cannot be...

journal_title：Microbial cell factories

authors： Hasunuma T,Hori Y,Sakamoto T,Ochiai M,Hatanaka H,Kondo A

更新日期：2014-10-12 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potenti...

journal_title：Microbial cell factories

authors： Yang YJ,Wang Y,Li ZF,Gong Y,Zhang P,Hu WC,Sheng DH,Li YZ

更新日期：2017-08-16 00:00:00

abstract：BACKGROUND:Targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the N-terminus of a secretory protein. These signal sequences usually contain an N-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signa...

journal_title：Microbial cell factories

authors： Yarimizu T,Nakamura M,Hoshida H,Akada R

更新日期：2015-02-14 00:00:00

abstract：BACKGROUND:The application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, ...

journal_title：Microbial cell factories

authors： Raddadi N,Giacomucci L,Totaro G,Fava F

更新日期：2017-11-02 00:00:00

abstract：BACKGROUND:Catalase (EC 1.11.1.6) is one of the important industrial enzyme employed in diagnostic and analytical methods in the form of biomarkers and biosensors in addition to their enormous applications in textile, paper, food and pharmaceutical sectors. The present study demonstrates the utility of a newly isolated...

journal_title：Microbial cell factories

authors： Kauldhar BS,Sooch BS

更新日期：2016-01-14 00:00:00

abstract：BACKGROUND:Glucose is the main carbon source of E. coli and a typical substrate in production processes. The main glucose uptake system is the glucose specific phosphotransferase system (Glc-PTS). The PTS couples glucose uptake with its phosphorylation. This is achieved by the concomitant conversion of phosphoenolpyruv...

journal_title：Microbial cell factories

authors： Kyselova L,Kreitmayer D,Kremling A,Bettenbrock K

更新日期：2018-08-28 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：: The aim of this work is to present a critical review on slurry bioreactors (SB) and their application to bioremediation of soils and sediments polluted with recalcitrant and toxic compounds. The scope of the review encompasses the following subjects: (i) process fundamentals of SB and analysis of advantages and disad...

journal_title：Microbial cell factories

authors： Robles-González IV,Fava F,Poggi-Varaldo HM

更新日期：2008-02-29 00:00:00

abstract：BACKGROUND:Bacillus subtilis has been widely used as a host for heterologous protein expression in food industry. B. subtilis ATCC6051 is an alternative expression host for the production of industrial enzymes, and exhibits favorable growth properties compared to B. subtilis 168. Extracellular expression of pullulanase...

journal_title：Microbial cell factories

authors： Liu X,Wang H,Wang B,Pan L

更新日期：2018-10-22 00:00:00

abstract：:Innovative new genome engineering technologies for manipulating chromosomes have appeared in the last decade. One of these technologies, recombination mediated genetic engineering (recombineering) allows for precision DNA engineering of chromosomes and plasmids in Escherichia coli. Single-stranded DNA recombineering (...

journal_title：Microbial cell factories

authors： van Pijkeren JP,Britton RA

更新日期：2014-08-29 00:00:00

abstract：BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...

journal_title：Microbial cell factories

authors： Zhang F,Qian X,Si H,Xu G,Han R,Ni Y

更新日期：2015-11-05 00:00:00

abstract：:Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...

journal_title：Microbial cell factories

authors： Villaverde A

更新日期：2010-07-05 00:00:00

abstract：: Microbes utilize enzymes to perform a variety of functions. Enzymes are biocatalysts working as highly efficient machines at the molecular level. In the past, enzymes have been viewed as static entities and their function has been explained on the basis of direct structural interactions between the enzyme and the sub...

journal_title：Microbial cell factories

authors： Agarwal PK

更新日期：2006-01-12 00:00:00

abstract：BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...

journal_title：Microbial cell factories

authors： de la Torre A,Metivier A,Chu F,Laurens LM,Beck DA,Pienkos PT,Lidstrom ME,Kalyuzhnaya MG

更新日期：2015-11-25 00:00:00

abstract：BACKGROUND:Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermophilus α-amylase in B...

journal_title：Microbial cell factories

authors： Yao D,Su L,Li N,Wu J

更新日期：2019-04-11 00:00:00

abstract：BACKGROUND:Cholesterol, the precursor of all steroid hormones, is the most abundant steroid in vertebrates and exhibits highly hydrophobic properties, rendering it a difficult substrate for aqueous microbial biotransformations. In the present study, we developed a Bacillus megaterium based whole-cell system that allows...

journal_title：Microbial cell factories

authors： Gerber A,Kleser M,Biedendieck R,Bernhardt R,Hannemann F

更新日期：2015-07-29 00:00:00