Abstract：
BACKGROUND:In past years research has focused on the development of alternative Gram positive bacterial expression systems to produce industrially relevant proteins. Brevibacillus choshinensis is an easy to handle non-sporulating bacterium, lacking extracellular proteases, that has been already shown to provide a high level of recombinant protein expression. One major drawback, limiting the applicability of the Brevibacillus expression system, is the absence of expression vectors based on inducible promoters. Here we used the PxylA inducible promoter, commonly employed in other Bacillae expression systems, in Brevibacillus. RESULTS:Using GFP, α-amylase and TcdA-GT as model proteins, high level of intracellular protein expression (up to 250 mg/L for the GFP) was achieved in Brevibacillus, using the pHis1522 vector carrying the B. megaterium xylose-inducible promoter (PxylA). The GFP expression yields were more than 25 fold higher than those reported for B. megaterium carrying the same vector. All the tested proteins show significant increment in their expression levels (2-10 folds) than those obtained using the available plasmids based on the P2 constitutive promoter. CONCLUSION:Combining the components of two different commercially available Gram positive expression systems, such as Brevibacillus (from Takara Bio) and B. megaterium (from Mobitec), we demonstrate that vectors based on the B. megaterium PxylA xylose inducible promoter can be successfully used to induce high level of intracellular expression of heterologous proteins in Brevibacillus.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
D'Urzo N,Martinelli M,Nenci C,Brettoni C,Telford JL,Maione Ddoi
10.1186/1475-2859-12-12subject
Has Abstractpub_date
2013-02-01 00:00:00pages
12issn
1475-2859pii
1475-2859-12-12journal_volume
12pub_type
杂志文章abstract：:Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use of strong methanol-...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-5-39
更新日期：2006-12-14 00:00:00
abstract：BACKGROUND:Activation of silent biosynthetic gene clusters (BGCs) in marine-derived actinomycete strains is a feasible strategy to discover bioactive natural products. Actinoalloteichus sp. AHMU CJ021, isolated from the seashore, was shown to contain an intact but silent caerulomycin A (CRM A) BGC-cam in its genome. Th...
journal_title：Microbial cell factories
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doi：10.1186/s12934-020-01418-w
更新日期：2020-08-06 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-1-5
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abstract：BACKGROUND:Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal intestinal flora is crucial for the development of intestinal inflammation. However, due to the complexity of the intest...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-6-18
更新日期：2007-06-06 00:00:00
abstract：BACKGROUND:Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by lignocellulose-degrading fungi including Pycnoporus cinnabarinus. We investigated the cellulolytic system of P. cinnabarinus, focusing on the involvement of CDH in the deconstruction of lignocellulosic biomass. RESULTS:First, P. ...
journal_title：Microbial cell factories
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doi：10.1186/1475-2859-10-113
更新日期：2011-12-28 00:00:00
abstract：BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...
journal_title：Microbial cell factories
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doi：10.1186/1475-2859-11-117
更新日期：2012-08-31 00:00:00
abstract：BACKGROUND:C4-dicarboxylic acids, including malic acid, fumaric acid and succinic acid, are valuable organic acids that can be produced and secreted by a number of microorganisms. Previous studies on organic acid production by Aspergillus carbonarius, which is capable of producing high amounts of citric acid from varie...
journal_title：Microbial cell factories
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更新日期：2017-03-14 00:00:00
abstract：BACKGROUND:(2S)-Pinocembrin is a chiral flavanone with versatile pharmacological and biological activities. Its health-promoting effects have spurred on research effects on the microbial production of (2S)-pinocembrin. However, an often-overlooked salient feature in the analysis of microbial (2S)-pinocembrin is its chi...
journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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更新日期：2019-02-07 00:00:00
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journal_title：Microbial cell factories
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doi：10.1186/1475-2859-10-36
更新日期：2011-05-18 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-58
更新日期：2012-05-09 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0538-z
更新日期：2016-08-11 00:00:00
abstract：BACKGROUND:L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS:In this study, Corynebacterium gl...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0776-8
更新日期：2017-09-22 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0504-9
更新日期：2016-06-08 00:00:00
abstract：BACKGROUND:A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS:Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. O...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-6-27
更新日期：2007-08-20 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-8-53
更新日期：2009-10-16 00:00:00
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journal_title：Microbial cell factories
pub_type： 社论
doi：10.1186/1475-2859-9-53
更新日期：2010-07-05 00:00:00
abstract：BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-161
更新日期：2012-12-15 00:00:00
abstract：BACKGROUND:Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis and hepatocellular carcinoma. Current therapies to treat HCV infection are shown to be partially effective and poorly tolerated. Therefore, ample efforts are underway to rationally design therapies targeting the HCV non-structural prot...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-12-111
更新日期：2013-11-16 00:00:00
abstract：BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0977-9
更新日期：2018-08-21 00:00:00
abstract：BACKGROUND:Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0693-x
更新日期：2017-05-02 00:00:00
abstract：BACKGROUND:Corynebacterium glutamicum is a well-known producer of various L-amino acids in industry. During the fermenting process, C. glutamicum unavoidably encounters oxidative stress due to a specific reactive oxygen species (ROS) produced by consistent adverse conditions. To combat the ROS, C. glutamicum has develo...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-1048-y
更新日期：2018-12-26 00:00:00
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pub_type： 杂志文章
doi：10.1186/s12934-020-01346-9
更新日期：2020-04-06 00:00:00
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pub_type： 杂志文章
doi：10.1186/s12934-017-0658-0
更新日期：2017-03-15 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0527-2
更新日期：2016-07-28 00:00:00
abstract：BACKGROUND:Chinese Nong-flavor (NF) liquor is continuously and stably produced by solid-state fermentation technology for 1000 years, resulting in enrichment of special microbial community and enzymes system in its starter. Based on traditional culture-dependent methods, these functional enzymes are hardly obtained. Ac...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0878-y
更新日期：2018-02-22 00:00:00
abstract：BACKGROUND:The Bacillus subtilis spore has long been used to display antigens and enzymes. Spore display can be accomplished by a recombinant and a non-recombinant approach, with the latter proved more efficient than the recombinant one. We used the non-recombinant approach to independently adsorb two thermophilic enzy...
journal_title：Microbial cell factories
pub_type： 杂志文章
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更新日期：2017-11-28 00:00:00
abstract：BACKGROUND:For the cultivation of Escherichia coli in bioreactors trace element solutions are generally designed for optimal growth under aerobic conditions. They do normally not contain selenium and nickel. Molybdenum is only contained in few of them. These elements are part of the formate hydrogen lyase (FHL) complex...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-7-26
更新日期：2008-08-07 00:00:00
abstract：BACKGROUND:Anaerobic digesters become unstable when operated at a high organi c loading rate (OLR). Investigating the microbial community response to OLR disturbance is helpful for achieving efficient and stable process operation. However, previous studies have only focused on community succession during different proc...
journal_title：Microbial cell factories
pub_type： 杂志文章
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更新日期：2016-04-25 00:00:00
abstract：BACKGROUND:Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0532-5
更新日期：2016-08-11 00:00:00
