解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
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神经系统科学
护理
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产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
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公共、环境和职业卫生
放射学,核医学和医学成像
康复学
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风湿病学
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热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been reported to have various in vivo and in vitro bio functionalities (e.g., anti-a...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01505-y
更新日期:2021-01-19 00:00:00
abstract:BACKGROUND:Saccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase (PDH) complex ex...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01493-z
更新日期:2020-12-10 00:00:00
abstract::All of humans and other mammalian species are colonized by some types of microorganisms such as bacteria, archaea, unicellular eukaryotes like fungi and protozoa, multicellular eukaryotes like helminths, and viruses, which in whole are called microbiota. These microorganisms have multiple different types of interactio...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/s12934-020-01483-1
更新日期:2020-11-26 00:00:00
abstract:BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01466-2
更新日期:2020-11-23 00:00:00
abstract:BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01458-2
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis in the filamentous fungal cell factory Aspergillus niger indicated that low a...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01450-w
更新日期:2020-10-23 00:00:00
abstract:BACKGROUND:In Saccharomyces cerevisiae, alpha-glucosidase (maltase) is a key enzyme in maltose metabolism. In addition, the overexpression of the alpha-glucosidase-encoding gene MAL62 has been shown to increase the freezing tolerance of yeast in lean dough. However, its cryoprotection mechanism is still not clear. RES...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01454-6
更新日期:2020-10-19 00:00:00
abstract:BACKGROUND:Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01448-4
更新日期:2020-10-07 00:00:00
abstract::Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of ce...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/s12934-020-01433-x
更新日期:2020-09-04 00:00:00
abstract:BACKGROUND:Cinnamyl alcohol is not only a kind of flavoring agent and fragrance, but also a versatile chemical applied in the production of various compounds. At present, the preparation of cinnamyl alcohol depends on plant extraction and chemical synthesis, which have several drawbacks, including limited scalability, ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01419-9
更新日期:2020-08-12 00:00:00
abstract:BACKGROUND:Activation of silent biosynthetic gene clusters (BGCs) in marine-derived actinomycete strains is a feasible strategy to discover bioactive natural products. Actinoalloteichus sp. AHMU CJ021, isolated from the seashore, was shown to contain an intact but silent caerulomycin A (CRM A) BGC-cam in its genome. Th...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01418-w
更新日期:2020-08-06 00:00:00
abstract:BACKGROUND:Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed. To this end, a crucial process is translation initiation which takes place at the ribosome binding s...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01404-2
更新日期:2020-07-29 00:00:00
abstract:BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01405-1
更新日期:2020-07-20 00:00:00
abstract:BACKGROUND:Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol production. Although Z. mobilis is robustly resilient to certain abiotic stress such as ethanol, ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01406-0
更新日期:2020-07-20 00:00:00
abstract:BACKGROUND:L-Alanyl-L-glutamine (AQ) is a functional dipeptide with high water solubility, good thermal stability and high bioavailability. It is widely used in clinical treatment, post-operative rehabilitation, sports health care and other fields. AQ is mainly produced via chemical synthesis which is complicated, time...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01369-2
更新日期:2020-06-11 00:00:00
abstract:BACKGROUND:Recombinant protein expression in bacteria often leads to the formation of intracellular insoluble protein deposits, a major bottleneck for the production of soluble and active products. However, in recent years, these bacterial protein aggregates, commonly known as inclusion bodies (IBs), have been shown to...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01375-4
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investiga...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01365-6
更新日期:2020-05-19 00:00:00
abstract:BACKGROUND:Trypsin has many applications in food and pharmaceutical manufacturing. Although commercial trypsin is usually extracted from porcine pancreas, this source carries the risks of infectivity and immunogenicity. Microbial Streptomyces griseus trypsin (SGT) is a prime alternative because it possesses efficient h...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01338-9
更新日期:2020-04-13 00:00:00
abstract::Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fu...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01346-9
更新日期:2020-04-06 00:00:00
abstract:BACKGROUND:Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, constituting direct repeats (D...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01321-4
更新日期:2020-03-10 00:00:00
abstract:BACKGROUND:Bacillus subtilis is an important industrial workhorse applied in the production of many different commercially relevant proteins, especially enzymes. Virtually all of these proteins are secreted via the general secretion (Sec) pathway. Studies from different laboratories have demonstrated essential or non-e...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01315-2
更新日期:2020-02-28 00:00:00
abstract:BACKGROUND:Spore-forming bacteria of the Bacillus genus are widely used probiotics known to exert their beneficial effects also through the stimulation of the host immune response. The oral delivery of B. toyonensis spores has been shown to improve the immune response to a parenterally administered viral antigen in mic...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01308-1
更新日期:2020-02-19 00:00:00
abstract::The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/s12934-020-1289-4
更新日期:2020-02-05 00:00:00
abstract:BACKGROUND:Histamine is a key mediator of the anti-inflammatory activity conferred by the probiotic organism Lactobacillus reuteri ATCC PTA 6475 in animal models of colitis and colorectal cancer. In L. reuteri, histamine synthesis and secretion requires L-histidine decarboxylase and a L-histidine/histamine exchanger. C...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1264-0
更新日期:2019-12-12 00:00:00
abstract:BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1257-z
更新日期:2019-11-25 00:00:00
abstract:BACKGROUND:The thermostable serine protease pernisine originates from the hyperthermophilic Archaeaon Aeropyrum pernix and has valuable industrial applications. Due to its properties, A. pernix cannot be cultivated in standard industrial fermentation facilities. Furthermore, pernisine is a demanding target for heterolo...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1245-3
更新日期:2019-11-07 00:00:00
abstract:BACKGROUND:Oxygen-evolving photoautotrophic organisms, like cyanobacteria, protect their photosynthetic machinery by a number of regulatory mechanisms, including alternative electron transfer pathways. Despite the importance in modulating the electron flux distribution between the photosystems, alternative electron tra...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1238-2
更新日期:2019-11-05 00:00:00
abstract:BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1213-y
更新日期:2019-10-10 00:00:00
abstract:BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1214-x
更新日期:2019-09-23 00:00:00
abstract:BACKGROUND:Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database. Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, s...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1212-z
更新日期:2019-09-21 00:00:00
abstract:BACKGROUND:Fine-tuning the aeration for cultivations when oxygen-limited conditions are demanded (such as the production of vaccines, isobutanol, 2-3 butanediol, acetone, and bioethanol) is still a challenge in the area of bioreactor automation and advanced control. In this work, an innovative control strategy based on...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1198-6
更新日期:2019-09-04 00:00:00
abstract:BACKGROUND:Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their superior physical and mechanical properties. We engineered a reversed fatty acid β-oxidation pathway i...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1186-x
更新日期:2019-08-13 00:00:00
abstract:BACKGROUND:Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing attention, but there were no reports of successful implementation. Escherichia coli has been engin...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1166-1
更新日期:2019-07-05 00:00:00
abstract:BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1164-3
更新日期:2019-06-28 00:00:00
abstract:BACKGROUND:Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hos...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1158-1
更新日期:2019-06-13 00:00:00
abstract:BACKGROUND:The oleaginous yeast Yarrowia lipolytica is an organism of choice for the tailored production of various compounds such as biofuels or biopolymers. When properly engineered, it is capable of producing medium-chain-length polyhydroxyalkanoate (mcl-PHA), a biobased and biodegradable polymer that can be used as...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1140-y
更新日期:2019-05-31 00:00:00
abstract:BACKGROUND:Cell surface display of recombinant proteins has become a powerful tool for biotechnology and biomedical applications. As a model eukaryotic microorganism, Saccharomyces cerevisiae is an ideal candidate for surface display of heterologous proteins. However, the frequently used commercial yeast surface displa...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1133-x
更新日期:2019-05-18 00:00:00
abstract:BACKGROUND:Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermophilus α-amylase in B...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1119-8
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1113-1
更新日期:2019-03-28 00:00:00
abstract:BACKGROUND:Due to their high energy density and compatible physical properties, several monoterpenes have been investigated as potential renewable transportation fuels, either as blendstocks with petroleum or as drop-in replacements for use in vehicles (both heavy and light-weight) or in aviation. Sustainable microbial...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1099-8
更新日期:2019-03-18 00:00:00