Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae.

Abstract:

BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate how Suc2 activity is regulated in S. cerevisiae. RESULTS:The effect of SUC2 expression level on inulin hydrolysis was investigated by introducing different SUC2 genes or their corresponding promoters in S. cerevisiae strain BY4741 that can only weakly catabolize inulin. Both inulinase and invertase activities were increased with the rising SUC2 expression level. Variation in the promoter sequence has an obvious effect on the transcript level of the SUC2 gene. It was also found that the high expression level of SUC2 was beneficial to inulin degradation and ethanol yield. CONCLUSIONS:Suc2-mediated inulin catabolism is regulated at transcript level in S. cerevisiae. Our work should be valuable for engineering advanced yeast strains in application of inulin for ethanol production.

journal_name

Microb Cell Fact

journal_title

Microbial cell factories

authors

Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

doi

10.1186/s12934-015-0243-3

subject

Has Abstract

pub_date

2015-04-17 00:00:00

pages

59

issn

1475-2859

pii

10.1186/s12934-015-0243-3

journal_volume

14

pub_type

杂志文章
  • An improved secretion signal enhances the secretion of model proteins from Pichia pastoris.

    abstract:BACKGROUND:Proteins can be secreted from a host organism with the aid of N-terminal secretion signals. The budding yeast Pichia pastoris (Komagataella sp.) is widely employed to secrete proteins of academic and industrial interest. For this yeast, the most commonly used secretion signal is the N-terminal portion of pre...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-1009-5

    authors: Barrero JJ,Casler JC,Valero F,Ferrer P,Glick BS

    更新日期:2018-10-12 00:00:00

  • Molecular characterisation of new organisation of plnEF and plw loci of bacteriocin genes harbour concomitantly in Lactobacillus plantarum I-UL4.

    abstract:BACKGROUND:Bacteriocin-producing Lactic acid bacteria (LAB) have vast applications in human and animal health, as well as in food industry. The structural, immunity, regulatory, export and modification genes are required for effective bacteriocin biosynthesis. Variations in gene sequence, composition and organisation w...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-015-0280-y

    authors: Tai HF,Foo HL,Abdul Rahim R,Loh TC,Abdullah MP,Yoshinobu K

    更新日期:2015-06-16 00:00:00

  • Metabolic engineering of Acremonium chrysogenum for improving cephalosporin C production independent of methionine stimulation.

    abstract:BACKGROUND:Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-0936-5

    authors: Liu J,Gao W,Pan Y,Liu G

    更新日期:2018-06-07 00:00:00

  • A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis.

    abstract:BACKGROUND:Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether prot...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-017-0688-7

    authors: Cai D,Wang H,He P,Zhu C,Wang Q,Wei X,Nomura CT,Chen S

    更新日期:2017-04-24 00:00:00

  • Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine.

    abstract:BACKGROUND:Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives i...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-11-138

    authors: Peifer S,Barduhn T,Zimmet S,Volmer DA,Heinzle E,Schneider K

    更新日期:2012-10-24 00:00:00

  • Identification and utilization of two important transporters: SgvT1 and SgvT2, for griseoviridin and viridogrisein biosynthesis in Streptomyces griseoviridis.

    abstract:BACKGROUND:Griseoviridin (GV) and viridogrisein (VG, also referred as etamycin), both biosynthesized by a distinct 105 kb biosynthetic gene cluster (BGC) in Streptomyces griseoviridis NRRL 2427, are a pair of synergistic streptogramin antibiotics and very important in treating infections of many multi-drug resistant mi...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-017-0792-8

    authors: Xie Y,Ma J,Qin X,Li Q,Ju J

    更新日期:2017-10-25 00:00:00

  • Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor.

    abstract:BACKGROUND:Trypsin has many applications in food and pharmaceutical manufacturing. Although commercial trypsin is usually extracted from porcine pancreas, this source carries the risks of infectivity and immunogenicity. Microbial Streptomyces griseus trypsin (SGT) is a prime alternative because it possesses efficient h...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-020-01338-9

    authors: Zhang Y,Liang Q,Zhang C,Zhang J,Du G,Kang Z

    更新日期:2020-04-13 00:00:00

  • Improved fatty aldehyde and wax ester production by overexpression of fatty acyl-CoA reductases.

    abstract:BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-0869-z

    authors: Lehtinen T,Efimova E,Santala S,Santala V

    更新日期:2018-02-08 00:00:00

  • Engineering building blocks for self-assembling protein nanoparticles.

    abstract::Like natural viruses, manmade protein cages for drug delivery are to be ideally formed by repetitive subunits with self-assembling properties, mimicking viral functions and molecular organization. Naturally formed nanostructures (such as viruses, flagella or simpler protein oligomers) can be engineered to acquire spec...

    journal_title:Microbial cell factories

    pub_type: 社论

    doi:10.1186/1475-2859-9-101

    authors: Vázquez E,Villaverde A

    更新日期:2010-12-30 00:00:00

  • Homologous high-throughput expression and purification of highly conserved E coli proteins.

    abstract:BACKGROUND:Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal intestinal flora is crucial for the development of intestinal inflammation. However, due to the complexity of the intest...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-6-18

    authors: Ergin A,Büssow K,Sieper J,Thiel A,Duchmann R,Adam T

    更新日期:2007-06-06 00:00:00

  • A review on slurry bioreactors for bioremediation of soils and sediments.

    abstract:: The aim of this work is to present a critical review on slurry bioreactors (SB) and their application to bioremediation of soils and sediments polluted with recalcitrant and toxic compounds. The scope of the review encompasses the following subjects: (i) process fundamentals of SB and analysis of advantages and disad...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-7-5

    authors: Robles-González IV,Fava F,Poggi-Varaldo HM

    更新日期:2008-02-29 00:00:00

  • Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.

    abstract:BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-1032-6

    authors: Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

    更新日期:2018-11-24 00:00:00

  • Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli.

    abstract:BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-10-15

    authors: Menzella HG

    更新日期:2011-03-03 00:00:00

  • Engineering Escherichia coli to convert acetic acid to β-caryophyllene.

    abstract:BACKGROUND:Under aerobic conditions, acetic acid is the major byproduct produced by E. coli during the fermentation. And acetic acid is detrimental to cell growth as it destroys transmembrane pH gradients. Hence, how to reduce the production of acetic acid and how to utilize it as a feedstock are of intriguing interest...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-016-0475-x

    authors: Yang J,Nie Q

    更新日期:2016-05-05 00:00:00

  • Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization.

    abstract:BACKGROUND:Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-1023-7

    authors: Faria C,Borges N,Rocha I,Santos H

    更新日期:2018-11-16 00:00:00

  • A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation.

    abstract:INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-1036-2

    authors: Haq SF,Shanbhag AP,Karthikeyan S,Hassan I,Thanukrishnan K,Ashok A,Sukumaran S,Ramaswamy S,Bharatham N,Datta S,Samant S,Katagihallimath N

    更新日期:2018-12-03 00:00:00

  • Engineering E. coli-E. coli cocultures for production of muconic acid from glycerol.

    abstract:BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-015-0319-0

    authors: Zhang H,Li Z,Pereira B,Stephanopoulos G

    更新日期:2015-09-15 00:00:00

  • Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger.

    abstract:BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-016-0527-2

    authors: Hossain AH,Li A,Brickwedde A,Wilms L,Caspers M,Overkamp K,Punt PJ

    更新日期:2016-07-28 00:00:00

  • Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels.

    abstract:BACKGROUND:In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understood reasons, the produc...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-12-24

    authors: Schlegel S,Rujas E,Ytterberg AJ,Zubarev RA,Luirink J,de Gier JW

    更新日期:2013-03-12 00:00:00

  • Increased salt tolerance in Zymomonas mobilis strain generated by adaptative evolution.

    abstract:BACKGROUND:Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol production. Although Z. mobilis is robustly resilient to certain abiotic stress such as ethanol, ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-020-01406-0

    authors: Fuchino K,Bruheim P

    更新日期:2020-07-20 00:00:00

  • Production of eicosapentaenoic acid by application of a delta-6 desaturase with the highest ALA catalytic activity in algae.

    abstract::Dunaliella salina is a unicellular green alga with a high α-linolenic acid (ALA) level, but a low eicosapentaenoic acid (EPA) level. In a previous analysis of the catalytic activity of delta 6 fatty acid desaturase (FADS6) from various species, FADS6 from Thalassiosira pseudonana (TpFADS6), a marine diatom, showed the...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-0857-3

    authors: Shi H,Luo X,Wu R,Yue X

    更新日期:2018-01-13 00:00:00

  • Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract:BACKGROUND:The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO2 for biotechnological applications, the genetic toolbox for Synechocystis 6803 is still a limited factor. RESULTS:We systematical...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-0897-8

    authors: Liu D,Pakrasi HB

    更新日期:2018-03-26 00:00:00

  • Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium.

    abstract:BACKGROUND:Cholesterol, the precursor of all steroid hormones, is the most abundant steroid in vertebrates and exhibits highly hydrophobic properties, rendering it a difficult substrate for aqueous microbial biotransformations. In the present study, we developed a Bacillus megaterium based whole-cell system that allows...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-015-0300-y

    authors: Gerber A,Kleser M,Biedendieck R,Bernhardt R,Hannemann F

    更新日期:2015-07-29 00:00:00

  • Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger.

    abstract:BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-13-11

    authors: van der Straat L,Vernooij M,Lammers M,van den Berg W,Schonewille T,Cordewener J,van der Meer I,Koops A,de Graaff LH

    更新日期:2014-01-17 00:00:00

  • Lactic acid fermentation as a tool to enhance the functional features of Echinacea spp.

    abstract:BACKGROUND:Extracts and products (roots and/or aerial parts) from Echinacea ssp. represent a profitable market sector for herbal medicines thanks to different functional features. Alkamides and polyacetylenes, phenols like caffeic acid and its derivatives, polysaccharides and glycoproteins are the main bioactive compou...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-12-44

    authors: Rizzello CG,Coda R,Macías DS,Pinto D,Marzani B,Filannino P,Giuliani G,Paradiso VM,Di Cagno R,Gobbetti M

    更新日期:2013-05-04 00:00:00

  • Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus.

    abstract:BACKGROUND:The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potenti...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-017-0758-x

    authors: Yang YJ,Wang Y,Li ZF,Gong Y,Zhang P,Hu WC,Sheng DH,Li YZ

    更新日期:2017-08-16 00:00:00

  • Mevalonate production from ethanol by direct conversion through acetyl-CoA using recombinant Pseudomonas putida, a novel biocatalyst for terpenoid production.

    abstract:BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-019-1213-y

    authors: Yang J,Son JH,Kim H,Cho S,Na JG,Yeon YJ,Lee J

    更新日期:2019-10-10 00:00:00

  • Microbial production host selection for converting second-generation feedstocks into bioproducts.

    abstract:BACKGROUND:Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors and salts that affect the performance of the microbial production host. The performance of six industr...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/1475-2859-8-64

    authors: Rumbold K,van Buijsen HJ,Overkamp KM,van Groenestijn JW,Punt PJ,van der Werf MJ

    更新日期:2009-12-04 00:00:00

  • Engineering the oleaginous yeast Yarrowia lipolytica to produce the aroma compound β-ionone.

    abstract:BACKGROUND:β-Ionone is a fragrant terpenoid that generates a pleasant floral scent and is used in diverse applications as a cosmetic and flavoring ingredient. A growing consumer desire for natural products has increased the market demand for natural β-ionone. To date, chemical extraction from plants remains the main ap...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-018-0984-x

    authors: Czajka JJ,Nathenson JA,Benites VT,Baidoo EEK,Cheng Q,Wang Y,Tang YJ

    更新日期:2018-09-01 00:00:00

  • Effect of aspartic acid and glutamate on metabolism and acid stress resistance of Acetobacter pasteurianus.

    abstract:BACKGROUND:Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the maj...

    journal_title:Microbial cell factories

    pub_type: 杂志文章

    doi:10.1186/s12934-017-0717-6

    authors: Yin H,Zhang R,Xia M,Bai X,Mou J,Zheng Y,Wang M

    更新日期:2017-06-15 00:00:00