Purification and characterization of a novel cold adapted fungal glucoamylase.


BACKGROUND:Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For example, the most commonly used industrial glucoamylases, a type of amylase that degrades starch to glucose, are produced by Aspergillus strains displaying optimal activities at 45-60 °C. Thus, isolating new amylases with optimal activity at ambient temperature is essential for improving industrial processes. In this report, a glucoamylase secreted by the cold-adapted yeast Tetracladium sp. was isolated and biochemically characterized. RESULTS:The effects of physicochemical parameters on enzyme activity were analyzed, and pH and temperature were found to be key factors modulating the glucoamylase activity. The optimal conditions for enzyme activity were 30 °C and pH 6.0, and the K m and k cat using soluble starch as substrate were 4.5 g/L and 45 min-1, respectively. Possible amylase or glucoamylase encoding genes were identified, and their transcript levels using glucose or soluble starch as the sole carbon source were analyzed. Transcription levels were highest in medium supplemented with soluble starch for the potential glucoamylase encoding gene. Comparison of the structural model of the identified Tetracladium sp. glucoamylase with the solved structure of the Hypocrea jecorina glucoamylase revealed unique structural features that may explain the thermal lability of the glucoamylase from Tetracladium sp. CONCLUSION:The glucoamylase secreted by Tetracladium sp. is a novel cold-adapted enzyme and its properties should render this enzyme suitable for use in industrial processes that require cold-active amylases, such as biofuel production.


Microb Cell Fact


Microbial cell factories


Carrasco M,Alcaíno J,Cifuentes V,Baeza M




Has Abstract


2017-05-02 00:00:00












  • Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid.

    abstract:BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Otto M,Teixeira PG,Vizcaino MI,David F,Siewers V

    更新日期:2019-11-25 00:00:00

  • ClC transporter activity modulates histidine catabolism in Lactobacillus reuteri by altering intracellular pH and membrane potential.

    abstract:BACKGROUND:Histamine is a key mediator of the anti-inflammatory activity conferred by the probiotic organism Lactobacillus reuteri ATCC PTA 6475 in animal models of colitis and colorectal cancer. In L. reuteri, histamine synthesis and secretion requires L-histidine decarboxylase and a L-histidine/histamine exchanger. C...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Hall AE,Engevik MA,Oezguen N,Haag A,Versalovic J

    更新日期:2019-12-12 00:00:00

  • Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus.

    abstract:BACKGROUND:The thermostable serine protease pernisine originates from the hyperthermophilic Archaeaon Aeropyrum pernix and has valuable industrial applications. Due to its properties, A. pernix cannot be cultivated in standard industrial fermentation facilities. Furthermore, pernisine is a demanding target for heterolo...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Šnajder M,Carrillo Rincón AF,Magdevska V,Bahun M,Kranjc L,Paš M,Juntes P,Petković H,Poklar Ulrih N

    更新日期:2019-11-07 00:00:00

  • Redirecting photosynthetic electron flux in the cyanobacterium Synechocystis sp. PCC 6803 by the deletion of flavodiiron protein Flv3.

    abstract:BACKGROUND:Oxygen-evolving photoautotrophic organisms, like cyanobacteria, protect their photosynthetic machinery by a number of regulatory mechanisms, including alternative electron transfer pathways. Despite the importance in modulating the electron flux distribution between the photosystems, alternative electron tra...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Thiel K,Patrikainen P,Nagy C,Fitzpatrick D,Pope N,Aro EM,Kallio P

    更新日期:2019-11-05 00:00:00

  • Production of Nα-acetylated thymosin α1 in Escherichia coli.

    abstract:BACKGROUND:Thymosin α1 (Tα1), a 28-amino acid Nα-acetylated peptide, has a powerful general immunostimulating activity. Although biosynthesis is an attractive means of large-scale manufacture, to date, Tα1 can only be chemosynthesized because of two obstacles to its biosynthesis: the difficulties in expressing small pe...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Ren Y,Yao X,Dai H,Li S,Fang H,Chen H,Zhou C

    更新日期:2011-04-22 00:00:00

  • Surface components and metabolites of probiotics for regulation of intestinal epithelial barrier.

    abstract::The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...

    journal_title:Microbial cell factories

    pub_type: 杂志文章,评审


    authors: Liu Q,Yu Z,Tian F,Zhao J,Zhang H,Zhai Q,Chen W

    更新日期:2020-02-05 00:00:00

  • Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli.

    abstract:BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Menzella HG

    更新日期:2011-03-03 00:00:00

  • Improved triacylglycerol production in Acinetobacter baylyi ADP1 by metabolic engineering.

    abstract:BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Santala S,Efimova E,Kivinen V,Larjo A,Aho T,Karp M,Santala V

    更新日期:2011-05-18 00:00:00

  • A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris.

    abstract:BACKGROUND:Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. To date, strain specific parameters, which are needed to set up feeding profiles for fed batch cultivations, are determined by time-consuming continuous cultures or consecutive ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Dietzsch C,Spadiut O,Herwig C

    更新日期:2011-03-03 00:00:00

  • Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyase.

    abstract:BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Papi RM,Chaitidou SA,Trikka FA,Kyriakidis DA

    更新日期:2005-12-14 00:00:00

  • Overexpression of a C4-dicarboxylate transporter is the key for rerouting citric acid to C4-dicarboxylic acid production in Aspergillus carbonarius.

    abstract:BACKGROUND:C4-dicarboxylic acids, including malic acid, fumaric acid and succinic acid, are valuable organic acids that can be produced and secreted by a number of microorganisms. Previous studies on organic acid production by Aspergillus carbonarius, which is capable of producing high amounts of citric acid from varie...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Yang L,Christakou E,Vang J,Lübeck M,Lübeck PS

    更新日期:2017-03-14 00:00:00

  • Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae.

    abstract:BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

    更新日期:2015-04-17 00:00:00

  • Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates.

    abstract::This article describes the use of biofilm reactors for the production of various chemicals by fermentation and wastewater treatment. Biofilm formation is a natural process where microbial cells attach to the support (adsorbent) or form flocs/aggregates (also called granules) without use of chemicals and form thick lay...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Qureshi N,Annous BA,Ezeji TC,Karcher P,Maddox IS

    更新日期:2005-08-25 00:00:00

  • A novel method to prepare L-arabinose from xylose mother liquor by yeast-mediated biopurification.

    abstract:BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Cheng H,Wang H,Lv J,Jiang M,Lin S,Deng Z

    更新日期:2011-06-07 00:00:00

  • A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC.

    abstract:BACKGROUND:Spore-forming bacteria of the Bacillus genus are widely used probiotics known to exert their beneficial effects also through the stimulation of the host immune response. The oral delivery of B. toyonensis spores has been shown to improve the immune response to a parenterally administered viral antigen in mic...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Santos FDS,Mazzoli A,Maia AR,Saggese A,Isticato R,Leite F,Iossa S,Ricca E,Baccigalupi L

    更新日期:2020-02-19 00:00:00

  • Production of eicosapentaenoic acid by application of a delta-6 desaturase with the highest ALA catalytic activity in algae.

    abstract::Dunaliella salina is a unicellular green alga with a high α-linolenic acid (ALA) level, but a low eicosapentaenoic acid (EPA) level. In a previous analysis of the catalytic activity of delta 6 fatty acid desaturase (FADS6) from various species, FADS6 from Thalassiosira pseudonana (TpFADS6), a marine diatom, showed the...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Shi H,Luo X,Wu R,Yue X

    更新日期:2018-01-13 00:00:00

  • Combination of uniform design with artificial neural network coupling genetic algorithm: an effective way to obtain high yield of biomass and algicidal compound of a novel HABs control actinomycete.

    abstract::Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Cai G,Zheng W,Yang X,Zhang B,Zheng T

    更新日期:2014-05-24 00:00:00

  • p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans.

    abstract:BACKGROUND:p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmf...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Kawai Y,Noda S,Ogino C,Takeshima Y,Okai N,Tanaka T,Kondo A

    更新日期:2013-05-07 00:00:00

  • Development of novel surface display platforms for anchoring heterologous proteins in Saccharomyces cerevisiae.

    abstract:BACKGROUND:Cell surface display of recombinant proteins has become a powerful tool for biotechnology and biomedical applications. As a model eukaryotic microorganism, Saccharomyces cerevisiae is an ideal candidate for surface display of heterologous proteins. However, the frequently used commercial yeast surface displa...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Yang X,Tang H,Song M,Shen Y,Hou J,Bao X

    更新日期:2019-05-18 00:00:00

  • Genetic manipulation of longevity-related genes as a tool to regulate yeast life span and metabolite production during winemaking.

    abstract:BACKGROUND:Yeast viability and vitality are essential for different industrial processes where the yeast Saccharomyces cerevisiae is used as a biotechnological tool. Therefore, the decline of yeast biological functions during aging may compromise their successful biotechnological use. Life span is controlled by a varie...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Orozco H,Matallana E,Aranda A

    更新日期:2013-01-02 00:00:00

  • Efficient production of extracellular pullulanase in Bacillus subtilis ATCC6051 using the host strain construction and promoter optimization expression system.

    abstract:BACKGROUND:Bacillus subtilis has been widely used as a host for heterologous protein expression in food industry. B. subtilis ATCC6051 is an alternative expression host for the production of industrial enzymes, and exhibits favorable growth properties compared to B. subtilis 168. Extracellular expression of pullulanase...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Liu X,Wang H,Wang B,Pan L

    更新日期:2018-10-22 00:00:00

  • Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum.

    abstract:BACKGROUND:A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS:Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. O...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Faijes M,Mars AE,Smid EJ

    更新日期:2007-08-20 00:00:00

  • The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis.

    abstract:BACKGROUND:Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed. To this end, a crucial process is translation initiation which takes place at the ribosome binding s...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Volkenborn K,Kuschmierz L,Benz N,Lenz P,Knapp A,Jaeger KE

    更新日期:2020-07-29 00:00:00

  • The functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit.

    abstract:BACKGROUND:We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investiga...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Butorac K,Banić M,Novak J,Leboš Pavunc A,Uroić K,Durgo K,Oršolić N,Kukolj M,Radović S,Scalabrin S,Žučko J,Starčević A,Šušković J,Kos B

    更新日期:2020-05-19 00:00:00

  • Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent.

    abstract:BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Upadhyay V,Singh A,Jha D,Singh A,Panda AK

    更新日期:2016-06-08 00:00:00

  • A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris.

    abstract:BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Rajamanickam V,Metzger K,Schmid C,Spadiut O

    更新日期:2017-09-13 00:00:00

  • Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure.

    abstract:BACKGROUND:The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intracellular protein for...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Pfeffer M,Maurer M,Köllensperger G,Hann S,Graf AB,Mattanovich D

    更新日期:2011-06-26 00:00:00

  • Bioprocess control in microscale: scalable fermentations in disposable and user-friendly microfluidic systems.

    abstract:BACKGROUND:The efficiency of biotechnological production processes depends on selecting the best performing microbial strain and the optimal cultivation conditions. Thus, many experiments have to be conducted, which conflicts with the demand to speed up drug development processes. Consequently, there is a great need fo...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Funke M,Buchenauer A,Mokwa W,Kluge S,Hein L,Müller C,Kensy F,Büchs J

    更新日期:2010-11-13 00:00:00

  • Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine.

    abstract:BACKGROUND:The rational design of L-phenylalanine (L-Phe) overproducing microorganisms has been successfully achieved by combining different genetic strategies such as inactivation of the phosphoenolpyruvate: phosphotransferase transport system (PTS) and overexpression of key genes (DAHP synthase, transketolase and cho...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Báez-Viveros JL,Flores N,Juárez K,Castillo-España P,Bolivar F,Gosset G

    更新日期:2007-09-19 00:00:00

  • Yeast-based assays for screening 11β-HSD1 inhibitors.

    abstract:BACKGROUND:Intracellular metabolism of glucocorticoid hormones plays an important role in the pathogenesis of metabolic syndrome and regulates, among many physiological processes, collagen metabolism in skin. At the peripheral level the concentration of active glucocorticoids is mainly regulated by the 11β-hydroxystero...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Vanella R,Callari R,Weston A,Heider H,Schwab MS,Kübler E

    更新日期:2016-03-15 00:00:00