当前位置： SCI文献检索 > Microbial Cell Factories期刊下所有文献 > Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes.

Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes.

Abstract：

BACKGROUND:Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by lignocellulose-degrading fungi including Pycnoporus cinnabarinus. We investigated the cellulolytic system of P. cinnabarinus, focusing on the involvement of CDH in the deconstruction of lignocellulosic biomass. RESULTS:First, P. cinnabarinus growth conditions were optimized for CDH production. Following growth under cellulolytic conditions, the main components secreted were cellulases, xylanases and CDH. To investigate the contribution of P. cinnabarinus secretome in saccharification processes, the Trichoderma reesei enzymatic cocktail was supplemented with the P. cinnabarinus secretome. A significant enhancement of the degradation of wheat straw was observed with (i) the production of a large amount of gluconic acid, (ii) increased hemicellulose degradation, and (iii) increased overall degradation of the lignocellulosic material. P. cinnabarinus CDH was heterologously expressed in Pichia pastoris to obtain large amounts of pure enzyme. In a bioreactor, the recombinant CDH (rCDH) expression level reached 7800 U/L. rCDH exhibited values of biochemical parameters similar to those of the natural enzyme, and was able to bind cellulose despite the absence of a carbohydrate-binding module (CBM). Following supplementation of purified rCDH to T. reesei enzymatic cocktail, formation of gluconic acid and increased hemicellulose degradation were observed, thus confirming the previous results observed with P. cinnabarinus secretome. CONCLUSIONS:We demonstrate that CDH offers an attractive tool for saccharification process enhancement due to gluconic acid production from raw lignocellulosic material.

journal_name

Microb Cell Fact

journal_title

Microbial cell factories

authors

Bey M,Berrin JG,Poidevin L,Sigoillot JC

doi

10.1186/1475-2859-10-113

subject

Has Abstract

pub_date

2011-12-28 00:00:00

pages

113

issn

1475-2859

pii

1475-2859-10-113

journal_volume

10

pub_type

杂志文章

相关文献

Microbial Cell Factories文献大全
  • ClC transporter activity modulates histidine catabolism in Lactobacillus reuteri by altering intracellular pH and membrane potential.

    abstract：BACKGROUND:Histamine is a key mediator of the anti-inflammatory activity conferred by the probiotic organism Lactobacillus reuteri ATCC PTA 6475 in animal models of colitis and colorectal cancer. In L. reuteri, histamine synthesis and secretion requires L-histidine decarboxylase and a L-histidine/histamine exchanger. C...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1264-0

    authors： Hall AE,Engevik MA,Oezguen N,Haag A,Versalovic J

    更新日期：2019-12-12 00:00:00

  • Production of biologically active human interleukin-10 by Bifidobacterium bifidum BGN4.

    abstract：BACKGROUND:Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been reported to have various in vivo and in vitro bio functionalities (e.g., anti-a...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01505-y

    authors： Hong N,Ku S,Yuk K,Johnston TV,Ji GE,Park MS

    更新日期：2021-01-19 00:00:00

  • Increased expression of the yeast multidrug resistance ABC transporter Pdr18 leads to increased ethanol tolerance and ethanol production in high gravity alcoholic fermentation.

    abstract：BACKGROUND:The understanding of the molecular basis of yeast tolerance to ethanol may guide the design of rational strategies to increase process performance in industrial alcoholic fermentations. A set of 21 genes encoding multidrug transporters from the ATP-Binding Cassette (ABC) Superfamily and Major Facilitator Sup...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-11-98

    authors： Teixeira MC,Godinho CP,Cabrito TR,Mira NP,Sá-Correia I

    更新日期：2012-07-27 00:00:00

  • Increased production of ganoderic acids by overexpression of homologous farnesyl diphosphate synthase and kinetic modeling of ganoderic acid production in Ganoderma lucidum.

    abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1164-3

    authors： Fei Y,Li N,Zhang DH,Xu JW

    更新日期：2019-06-28 00:00:00

  • Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyase.

    abstract：BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-4-35

    authors： Papi RM,Chaitidou SA,Trikka FA,Kyriakidis DA

    更新日期：2005-12-14 00:00:00

  • Systematic pathway engineering of Corynebacterium glutamicum S9114 for L-ornithine production.

    abstract：BACKGROUND:L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS:In this study, Corynebacterium gl...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0776-8

    authors： Zhang B,Yu M,Zhou Y,Li Y,Ye BC

    更新日期：2017-09-22 00:00:00

  • Redirecting photosynthetic electron flux in the cyanobacterium Synechocystis sp. PCC 6803 by the deletion of flavodiiron protein Flv3.

    abstract：BACKGROUND:Oxygen-evolving photoautotrophic organisms, like cyanobacteria, protect their photosynthetic machinery by a number of regulatory mechanisms, including alternative electron transfer pathways. Despite the importance in modulating the electron flux distribution between the photosystems, alternative electron tra...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1238-2

    authors： Thiel K,Patrikainen P,Nagy C,Fitzpatrick D,Pope N,Aro EM,Kallio P

    更新日期：2019-11-05 00:00:00

  • Genetic manipulation of longevity-related genes as a tool to regulate yeast life span and metabolite production during winemaking.

    abstract：BACKGROUND:Yeast viability and vitality are essential for different industrial processes where the yeast Saccharomyces cerevisiae is used as a biotechnological tool. Therefore, the decline of yeast biological functions during aging may compromise their successful biotechnological use. Life span is controlled by a varie...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-12-1

    authors： Orozco H,Matallana E,Aranda A

    更新日期：2013-01-02 00:00:00

  • Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae.

    abstract：BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0243-3

    authors： Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

    更新日期：2015-04-17 00:00:00

  • Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter.

    abstract：BACKGROUND:Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages, which is why current ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0292-7

    authors： Capone S,Horvat J,Herwig C,Spadiut O

    更新日期：2015-07-10 00:00:00

  • Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference.

    abstract：BACKGROUND:The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-018-0864-4

    authors： Huo YY,Jian SL,Cheng H,Rong Z,Cui HL,Xu XW

    更新日期：2018-01-30 00:00:00

  • Carbohydrate catabolic flexibility in the mammalian intestinal commensal Lactobacillus ruminis revealed by fermentation studies aligned to genome annotations.

    abstract：BACKGROUND:Lactobacillus ruminis is a poorly characterized member of the Lactobacillus salivarius clade that is part of the intestinal microbiota of pigs, humans and other mammals. Its variable abundance in human and animals may be linked to historical changes over time and geographical differences in dietary intake of...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-10-S1-S12

    authors： O'Donnell MM,Forde BM,Neville B,Ross PR,O'Toole PW

    更新日期：2011-08-30 00:00:00

  • Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger.

    abstract：BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-13-11

    authors： van der Straat L,Vernooij M,Lammers M,van den Berg W,Schonewille T,Cordewener J,van der Meer I,Koops A,de Graaff LH

    更新日期：2014-01-17 00:00:00

  • A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation.

    abstract：INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-018-1036-2

    authors： Haq SF,Shanbhag AP,Karthikeyan S,Hassan I,Thanukrishnan K,Ashok A,Sukumaran S,Ramaswamy S,Bharatham N,Datta S,Samant S,Katagihallimath N

    更新日期：2018-12-03 00:00:00

  • Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae.

    abstract：BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01405-1

    authors： Qiu C,Chen X,Rexida R,Shen Y,Qi Q,Bao X,Hou J

    更新日期：2020-07-20 00:00:00

  • Metabolic regulation of Escherichia coli and its gdhA, glnL, gltB, D mutants under different carbon and nitrogen limitations in the continuous culture.

    abstract：BACKGROUND:It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of practical interest for the heterologous protein production, PHB production, etc. Although the carbon and...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-9-8

    authors： Kumar R,Shimizu K

    更新日期：2010-01-27 00:00:00

  • A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris.

    abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0768-8

    authors： Rajamanickam V,Metzger K,Schmid C,Spadiut O

    更新日期：2017-09-13 00:00:00

  • Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice.

    abstract：BACKGROUND:Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. METHODS:We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxy...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0378-2

    authors： Shigemori S,Watanabe T,Kudoh K,Ihara M,Nigar S,Yamamoto Y,Suda Y,Sato T,Kitazawa H,Shimosato T

    更新日期：2015-11-25 00:00:00

  • Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures.

    abstract：BACKGROUND:The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. Recently, an HPLC-MS-based method to quantify IPTG in the medium and...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-11-58

    authors： Fernández-Castané A,Caminal G,López-Santín J

    更新日期：2012-05-09 00:00:00

  • Engineering E. coli-E. coli cocultures for production of muconic acid from glycerol.

    abstract：BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0319-0

    authors： Zhang H,Li Z,Pereira B,Stephanopoulos G

    更新日期：2015-09-15 00:00:00

  • Enantioselective cascade biocatalysis for deracemization of 2-hydroxy acids using a three-enzyme system.

    abstract：BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-016-0560-1

    authors： Xue YP,Zeng H,Jin XL,Liu ZQ,Zheng YG

    更新日期：2016-09-22 00:00:00

  • The functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit.

    abstract：BACKGROUND:We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investiga...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01365-6

    authors： Butorac K,Banić M,Novak J,Leboš Pavunc A,Uroić K,Durgo K,Oršolić N,Kukolj M,Radović S,Scalabrin S,Žučko J,Starčević A,Šušković J,Kos B

    更新日期：2020-05-19 00:00:00

  • Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.

    abstract：: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been th...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-7-11

    authors： Gasser B,Saloheimo M,Rinas U,Dragosits M,Rodríguez-Carmona E,Baumann K,Giuliani M,Parrilli E,Branduardi P,Lang C,Porro D,Ferrer P,Tutino ML,Mattanovich D,Villaverde A

    更新日期：2008-04-04 00:00:00

  • Rational selection and engineering of exogenous principal sigma factor (σ(HrdB)) to increase teicoplanin production in an industrial strain of Actinoplanes teichomyceticus.

    abstract：BACKGROUND:Transcriptional engineering has presented a strong ability of phenotypic improvement in microorganisms. However, it could not be directly applied to Actinoplanes teichomyceticus L-27 because of the paucity of endogenous transcription factors in the strain. In this study, exogenous transcription factors were ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-13-10

    authors： Wang H,Yang L,Wu K,Li G

    更新日期：2014-01-16 00:00:00

  • Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers.

    abstract：BACKGROUND:Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their superior physical and mechanical properties. We engineered a reversed fatty acid β-oxidation pathway i...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1186-x

    authors： Zhuang Q,Qi Q

    更新日期：2019-08-13 00:00:00

  • Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans.

    abstract：BACKGROUND:Lipases (EC 3.1.1.3) catalyze the hydrolysis of triacyl glycerol to glycerol and are involved in the synthesis of both short chain and long chain acylglycerols. They are widely used industrially in various applications, such as baking, laundry detergents and as biocatalysts in alternative energy strategies. ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-11-72

    authors： Selvin J,Kennedy J,Lejon DP,Kiran GS,Dobson AD

    更新日期：2012-06-01 00:00:00

  • Nanotechnology, bionanotechnology and microbial cell factories.

    abstract：:Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...

    journal_title：Microbial cell factories

    pub_type： 社论

    doi：10.1186/1475-2859-9-53

    authors： Villaverde A

    更新日期：2010-07-05 00:00:00

  • Characterization of a promiscuous cadmium and arsenic resistance mechanism in Thermus thermophilus HB27 and potential application of a novel bioreporter system.

    abstract：BACKGROUND:The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation. In this context, the bacterium Thermus thermophilus HB27 is a metal tolerant thermophile containing a set of genes involved in arsenic resistance which, differen...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-018-0918-7

    authors： Antonucci I,Gallo G,Limauro D,Contursi P,Ribeiro AL,Blesa A,Berenguer J,Bartolucci S,Fiorentino G

    更新日期：2018-05-18 00:00:00

  • A mesophilic anaerobic digester for treating food waste: process stability and microbial community analysis using pyrosequencing.

    abstract：BACKGROUND:Anaerobic digesters become unstable when operated at a high organi c loading rate (OLR). Investigating the microbial community response to OLR disturbance is helpful for achieving efficient and stable process operation. However, previous studies have only focused on community succession during different proc...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-016-0466-y

    authors： Li L,He Q,Ma Y,Wang X,Peng X

    更新日期：2016-04-25 00:00:00

  • Succinic acid production with Actinobacillus succinogenes: rate and yield analysis of chemostat and biofilm cultures.

    abstract：BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0111-6

    authors： Brink HG,Nicol W

    更新日期：2014-08-19 00:00:00