Abstract：
BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expression requires choosing from a vast number of possible DNA sequences and different codon optimization methods have been used in the past decade. Here, a comparative study of the two most common methods is presented using calf prochymosin as a model. RESULTS:Seven sequences encoding calf prochymosin have been designed, two using the "one amino acid-one codon" method and five using a "codon randomization" strategy. When expressed in Escherichia coli, the variants optimized by the codon randomization approach produced significantly more proteins than the native sequence including one gene that produced an increase of 70% in the amount of prochymosin accumulated. On the other hand, no significant improvement in protein expression was observed for the variants designed with the one amino acid-one codon method. The use of codon-optimized sequences did not affect the quality of the recovered inclusion bodies. CONCLUSIONS:The results obtained in this study indicate that the codon randomization method is a superior strategy for codon optimization. A significant improvement in protein expression was obtained for the largely established process of chymosin production, showing the power of this strategy to reduce production costs of industrial enzymes in microbial hosts.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Menzella HGdoi
10.1186/1475-2859-10-15subject
Has Abstractpub_date
2011-03-03 00:00:00pages
15issn
1475-2859pii
1475-2859-10-15journal_volume
10pub_type
杂志文章abstract：BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...
journal_title：Microbial cell factories
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doi：10.1186/s12934-019-1245-3
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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更新日期：2012-04-30 00:00:00
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journal_title：Microbial cell factories
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更新日期：2020-07-20 00:00:00
abstract：BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-10-43
更新日期：2011-06-07 00:00:00
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-10-14
更新日期：2011-03-03 00:00:00
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journal_title：Microbial cell factories
pub_type： 社论
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更新日期：2013-01-14 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0776-8
更新日期：2017-09-22 00:00:00
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doi：10.1186/s12934-015-0280-y
更新日期：2015-06-16 00:00:00
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journal_title：Microbial cell factories
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doi：10.1186/s12934-020-01419-9
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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journal_title：Microbial cell factories
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doi：10.1186/s12934-016-0466-y
更新日期：2016-04-25 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
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更新日期：2019-11-25 00:00:00
abstract：BACKGROUND:Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose r...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0982-z
更新日期：2018-08-29 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-014-0166-4
更新日期：2014-12-03 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-12-115
更新日期：2013-11-19 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-13-75
更新日期：2014-05-24 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0292-7
更新日期：2015-07-10 00:00:00
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journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0532-5
更新日期：2016-08-11 00:00:00
abstract：BACKGROUND:The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have problems such as screeni...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-9-30
更新日期：2010-05-11 00:00:00
