Increased salt tolerance in Zymomonas mobilis strain generated by adaptative evolution.

abstract：BACKGROUND:Streptomycetes produce a plethora of natural products including antibiotics and anticancer drugs, as well as many industrial enzymes. Their mycelial life style is a major bottleneck for industrial exploitation and over decades strain improvement programs have selected production strains with better growth pr...

journal_title：Microbial cell factories

authors： van Dissel D,Claessen D,Roth M,van Wezel GP

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...

journal_title：Microbial cell factories

authors： Bongers M,Chrysanthopoulos PK,Behrendorff JB,Hodson MP,Vickers CE,Nielsen LK

更新日期：2015-11-26 00:00:00

abstract：BACKGROUND:Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In f...

journal_title：Microbial cell factories

authors： Csörgo B,Fehér T,Tímár E,Blattner FR,Pósfai G

更新日期：2012-01-20 00:00:00

abstract：BACKGROUND:Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hos...

journal_title：Microbial cell factories

authors： Jin H,Jeong Y,Yoo SH,Johnston TV,Ku S,Ji GE

更新日期：2019-06-13 00:00:00

abstract：BACKGROUND:Transcriptional engineering has presented a strong ability of phenotypic improvement in microorganisms. However, it could not be directly applied to Actinoplanes teichomyceticus L-27 because of the paucity of endogenous transcription factors in the strain. In this study, exogenous transcription factors were ...

journal_title：Microbial cell factories

authors： Wang H,Yang L,Wu K,Li G

更新日期：2014-01-16 00:00:00

abstract：BACKGROUND:Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications deriv...

journal_title：Microbial cell factories

authors： Sun H,Zhao D,Xiong B,Zhang C,Bi C

更新日期：2016-08-24 00:00:00

abstract：BACKGROUND:Spore-forming bacteria of the Bacillus genus are widely used probiotics known to exert their beneficial effects also through the stimulation of the host immune response. The oral delivery of B. toyonensis spores has been shown to improve the immune response to a parenterally administered viral antigen in mic...

journal_title：Microbial cell factories

authors： Santos FDS,Mazzoli A,Maia AR,Saggese A,Isticato R,Leite F,Iossa S,Ricca E,Baccigalupi L

更新日期：2020-02-19 00:00:00

abstract：:Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features...

journal_title：Microbial cell factories

authors： Song AA,In LLA,Lim SHE,Rahim RA

更新日期：2017-04-04 00:00:00

abstract：BACKGROUND:Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward...

journal_title：Microbial cell factories

authors： Faria C,Borges N,Rocha I,Santos H

更新日期：2018-11-16 00:00:00

abstract：BACKGROUND:Acetic acid is a byproduct of Saccharomyces cerevisiae alcoholic fermentation. Together with high concentrations of ethanol and other toxic metabolites, acetic acid may contribute to fermentation arrest and reduced ethanol productivity. This weak acid is also a present in lignocellulosic hydrolysates, a high...

journal_title：Microbial cell factories

authors： Mira NP,Palma M,Guerreiro JF,Sá-Correia I

更新日期：2010-10-25 00:00:00

abstract：BACKGROUND:Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the mitochondrial thiol oxidase ERV1 gene was the most effective for high GSSG production in Saccharomyc...

journal_title：Microbial cell factories

authors： Kobayashi J,Sasaki D,Hara KY,Hasunuma T,Kondo A

更新日期：2017-03-15 00:00:00

abstract：BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...

journal_title：Microbial cell factories

authors： Cheng H,Wang H,Lv J,Jiang M,Lin S,Deng Z

更新日期：2011-06-07 00:00:00

abstract：BACKGROUND:Proteins can be secreted from a host organism with the aid of N-terminal secretion signals. The budding yeast Pichia pastoris (Komagataella sp.) is widely employed to secrete proteins of academic and industrial interest. For this yeast, the most commonly used secretion signal is the N-terminal portion of pre...

journal_title：Microbial cell factories

authors： Barrero JJ,Casler JC,Valero F,Ferrer P,Glick BS

更新日期：2018-10-12 00:00:00

abstract：BACKGROUND:Streptomyces venezuelae ATCC 10712 produces antibiotics chloramphenicol (Cml) and jadomycin (Jad) in response to nutrient limitation and ethanol shock (ES), respectively. Biosynthesis of Cml and Jad was shown to be reciprocally regulated via the action of regulatory proteins JadR1 and JadR2 encoded by the ja...

journal_title：Microbial cell factories

authors： Sekurova ON,Zhang J,Kristiansen KA,Zotchev SB

更新日期：2016-05-20 00:00:00

abstract：BACKGROUND:In spite of the substantial metabolic engineering effort previously devoted to the development of Saccharomyces cerevisiae strains capable of fermenting both the hexose and pentose sugars present in lignocellulose hydrolysates, the productivity of reported strains for conversion of the naturally most abundan...

journal_title：Microbial cell factories

authors： Krahulec S,Petschacher B,Wallner M,Longus K,Klimacek M,Nidetzky B

更新日期：2010-03-10 00:00:00

abstract：:The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...

journal_title：Microbial cell factories

authors： Liu Q,Yu Z,Tian F,Zhao J,Zhang H,Zhai Q,Chen W

更新日期：2020-02-05 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potenti...

journal_title：Microbial cell factories

authors： Yang YJ,Wang Y,Li ZF,Gong Y,Zhang P,Hu WC,Sheng DH,Li YZ

更新日期：2017-08-16 00:00:00

abstract：:Recent evidence has demonstrated that cell-to-cell signaling is a fundamental activity carried out by numerous microorganisms. A number of specialized processes are reported to be regulated by density-dependent signaling molecules including antibiotic production, bioluminescence, biofilm formation, genetic competence,...

journal_title：Microbial cell factories

authors： DeLisa MP,Bentley WE

更新日期：2002-12-20 00:00:00

abstract：BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...

journal_title：Microbial cell factories

authors： Wu S,Gu W,Huang A,Li Y,Kumar M,Lim PE,Huan L,Gao S,Wang G

更新日期：2019-09-23 00:00:00

abstract：BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...

journal_title：Microbial cell factories

authors： Nowrouzi B,Li RA,Walls LE,d'Espaux L,Malcı K,Liang L,Jonguitud-Borrego N,Lerma-Escalera AI,Morones-Ramirez JR,Keasling JD,Rios-Solis L

更新日期：2020-11-02 00:00:00

abstract：:Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In t...

journal_title：Microbial cell factories

authors： Yan S,Wu G

更新日期：2017-07-19 00:00:00

abstract：BACKGROUND:Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing attention, but there were no reports of successful implementation. Escherichia coli has been engin...

journal_title：Microbial cell factories

authors： Wang W,He P,Zhao D,Ye L,Dai L,Zhang X,Sun Y,Zheng J,Bi C

更新日期：2019-07-05 00:00:00

abstract：:Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use of strong methanol-...

journal_title：Microbial cell factories

authors： Hartner FS,Glieder A

更新日期：2006-12-14 00:00:00

abstract：BACKGROUND:Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages, which is why current ...

journal_title：Microbial cell factories

authors： Capone S,Horvat J,Herwig C,Spadiut O

更新日期：2015-07-10 00:00:00

abstract：BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...

journal_title：Microbial cell factories

authors： Brink HG,Nicol W

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...

journal_title：Microbial cell factories

authors： Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

更新日期：2015-04-17 00:00:00

abstract：BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...

journal_title：Microbial cell factories

authors： Yang CH,Liu EJ,Chen YL,Ou-Yang FY,Li SY

更新日期：2016-08-02 00:00:00

abstract：BACKGROUND:The solubility of recombinant proteins expressed in bacteria is often disappointingly low. Several strategies have been developed to improve the yield and one of the most common strategies is the fusion of the target protein with a suitable partner. Despite several reports on the successful use of each of th...

journal_title：Microbial cell factories

authors： Dümmler A,Lawrence AM,de Marco A

更新日期：2005-12-13 00:00:00

abstract：BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...

journal_title：Microbial cell factories

authors： Yang J,Son JH,Kim H,Cho S,Na JG,Yeon YJ,Lee J

更新日期：2019-10-10 00:00:00

abstract：BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...

journal_title：Microbial cell factories

authors： Silva R,Aguiar TQ,Coelho E,Jiménez A,Revuelta JL,Domingues L

更新日期：2019-03-28 00:00:00