A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC.

abstract：BACKGROUND:Rhodosporidium and Rhodotorula are two genera of oleaginous red yeast with great potential for industrial biotechnology. To date, there is no effective method for inducible expression of proteins and RNAs in these hosts. RESULTS:We have developed a luciferase gene reporter assay based on a new codon-optimiz...

journal_title：Microbial cell factories

authors： Liu Y,Koh CMJ,Ngoh ST,Ji L

更新日期：2015-10-26 00:00:00

abstract：BACKGROUND:To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-thr...

journal_title：Microbial cell factories

authors： Navarro D,Couturier M,da Silva GG,Berrin JG,Rouau X,Asther M,Bignon C

更新日期：2010-07-16 00:00:00

abstract：:Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...

journal_title：Microbial cell factories

authors： Cai G,Zheng W,Yang X,Zhang B,Zheng T

更新日期：2014-05-24 00:00:00

abstract：BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...

journal_title：Microbial cell factories

authors： Cheng H,Wang H,Lv J,Jiang M,Lin S,Deng Z

更新日期：2011-06-07 00:00:00

abstract：BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...

journal_title：Microbial cell factories

authors： Wu S,Gu W,Huang A,Li Y,Kumar M,Lim PE,Huan L,Gao S,Wang G

更新日期：2019-09-23 00:00:00

abstract：BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

journal_title：Microbial cell factories

authors： Menzella HG

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...

journal_title：Microbial cell factories

authors： Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

更新日期：2012-11-04 00:00:00

abstract：BACKGROUND:Mucosal delivery of therapeutic proteins using genetically modified strains of lactic acid bacteria (gmLAB) is being investigated as a new therapeutic strategy. METHODS:We developed a strain of gmLAB, Lactococcus lactis NZ9000 (NZ-HO), which secretes the anti-inflammatory molecule recombinant mouse heme oxy...

journal_title：Microbial cell factories

authors： Shigemori S,Watanabe T,Kudoh K,Ihara M,Nigar S,Yamamoto Y,Suda Y,Sato T,Kitazawa H,Shimosato T

更新日期：2015-11-25 00:00:00

abstract：BACKGROUND:The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation. In this context, the bacterium Thermus thermophilus HB27 is a metal tolerant thermophile containing a set of genes involved in arsenic resistance which, differen...

journal_title：Microbial cell factories

authors： Antonucci I,Gallo G,Limauro D,Contursi P,Ribeiro AL,Blesa A,Berenguer J,Bartolucci S,Fiorentino G

更新日期：2018-05-18 00:00:00

abstract：BACKGROUND:The rational design of L-phenylalanine (L-Phe) overproducing microorganisms has been successfully achieved by combining different genetic strategies such as inactivation of the phosphoenolpyruvate: phosphotransferase transport system (PTS) and overexpression of key genes (DAHP synthase, transketolase and cho...

journal_title：Microbial cell factories

authors： Báez-Viveros JL,Flores N,Juárez K,Castillo-España P,Bolivar F,Gosset G

更新日期：2007-09-19 00:00:00

abstract：BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...

journal_title：Microbial cell factories

authors： Yue YC,Yang BY,Lu J,Zhang SW,Liu L,Nassar K,Xu XX,Pang XY,Lv JP

更新日期：2020-11-23 00:00:00

abstract：BACKGROUND:In past years research has focused on the development of alternative Gram positive bacterial expression systems to produce industrially relevant proteins. Brevibacillus choshinensis is an easy to handle non-sporulating bacterium, lacking extracellular proteases, that has been already shown to provide a high ...

journal_title：Microbial cell factories

authors： D'Urzo N,Martinelli M,Nenci C,Brettoni C,Telford JL,Maione D

更新日期：2013-02-01 00:00:00

abstract：BACKGROUND:A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered ...

journal_title：Microbial cell factories

authors： Liu JL,Shriver-Lake LC,Anderson GP,Zabetakis D,Goldman ER

更新日期：2017-12-12 00:00:00

abstract：BACKGROUND:Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether prot...

journal_title：Microbial cell factories

authors： Cai D,Wang H,He P,Zhu C,Wang Q,Wei X,Nomura CT,Chen S

更新日期：2017-04-24 00:00:00

abstract：BACKGROUND:Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. To date, strain specific parameters, which are needed to set up feeding profiles for fed batch cultivations, are determined by time-consuming continuous cultures or consecutive ...

journal_title：Microbial cell factories

authors： Dietzsch C,Spadiut O,Herwig C

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complica...

journal_title：Microbial cell factories

authors： Zhu Y,Hua Y,Zhang B,Sun L,Li W,Kong X,Hong J

更新日期：2017-01-03 00:00:00

abstract：BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...

journal_title：Microbial cell factories

authors： Xue YP,Zeng H,Jin XL,Liu ZQ,Zheng YG

更新日期：2016-09-22 00:00:00

abstract：BACKGROUND:In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration (FDA)-approved for human applications. Widespread use of E. coli in ...

journal_title：Microbial cell factories

authors： Marisch K,Bayer K,Cserjan-Puschmann M,Luchner M,Striedner G

更新日期：2013-06-11 00:00:00

abstract：:The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...

journal_title：Microbial cell factories

authors： Liu Q,Yu Z,Tian F,Zhao J,Zhang H,Zhai Q,Chen W

更新日期：2020-02-05 00:00:00

abstract：BACKGROUND:Most bacteria can use various compounds as carbon sources. These carbon sources can be either co-metabolized or sequentially metabolized, where the latter phenomenon typically occurs as catabolite repression. From the practical application point of view of utilizing lignocellulose for the production of biofu...

journal_title：Microbial cell factories

authors： Yao R,Hirose Y,Sarkar D,Nakahigashi K,Ye Q,Shimizu K

更新日期：2011-08-11 00:00:00

abstract：BACKGROUND:The Gram-positive bacterium Bacillus subtilis has been widely used as a cell factory for the production of proteins due to its generally regarded as safe (GRAS) nature and secretion capability. Of the known secretory pathways in B. subtilis, the majority of proteins are exported from the cytoplasm by Sec pat...

journal_title：Microbial cell factories

authors： Chen J,Zhao L,Fu G,Zhou W,Sun Y,Zheng P,Sun J,Zhang D

更新日期：2016-04-28 00:00:00

abstract：BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...

journal_title：Microbial cell factories

authors： Rohe P,Venkanna D,Kleine B,Freudl R,Oldiges M

更新日期：2012-10-31 00:00:00

abstract：BACKGROUND:Histamine is a key mediator of the anti-inflammatory activity conferred by the probiotic organism Lactobacillus reuteri ATCC PTA 6475 in animal models of colitis and colorectal cancer. In L. reuteri, histamine synthesis and secretion requires L-histidine decarboxylase and a L-histidine/histamine exchanger. C...

journal_title：Microbial cell factories

authors： Hall AE,Engevik MA,Oezguen N,Haag A,Versalovic J

更新日期：2019-12-12 00:00:00

abstract：: Microbes utilize enzymes to perform a variety of functions. Enzymes are biocatalysts working as highly efficient machines at the molecular level. In the past, enzymes have been viewed as static entities and their function has been explained on the basis of direct structural interactions between the enzyme and the sub...

journal_title：Microbial cell factories

authors： Agarwal PK

更新日期：2006-01-12 00:00:00

abstract：BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...

journal_title：Microbial cell factories

authors： Yang CH,Liu EJ,Chen YL,Ou-Yang FY,Li SY

更新日期：2016-08-02 00:00:00

abstract：BACKGROUND:The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H:gm. The SefA pro...

journal_title：Microbial cell factories

authors： Jarmander J,Gustavsson M,Do TH,Samuelson P,Larsson G

更新日期：2012-09-03 00:00:00

abstract：BACKGROUND:The understanding of the molecular basis of yeast tolerance to ethanol may guide the design of rational strategies to increase process performance in industrial alcoholic fermentations. A set of 21 genes encoding multidrug transporters from the ATP-Binding Cassette (ABC) Superfamily and Major Facilitator Sup...

journal_title：Microbial cell factories

authors： Teixeira MC,Godinho CP,Cabrito TR,Mira NP,Sá-Correia I

更新日期：2012-07-27 00:00:00

abstract：BACKGROUND:A cellular stress response is triggered upon induction of recombinant protein expression which feedback inhibits both growth as well as protein synthesis. In order to separate these two effects, it was decided to study "quiescent cultures" which continue to be metabolically active and express recombinant pro...

journal_title：Microbial cell factories

authors： Mahalik S,Sharma AK,Jain P,Mukherjee KJ

更新日期：2017-07-28 00:00:00

abstract：BACKGROUND:Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an ...

journal_title：Microbial cell factories

authors： Dong W,Cai Y,Xu Z,Fu B,Chen Q,Cui Y,Ruan Z,Liang Y,Peng N,Zhao S

更新日期：2020-10-07 00:00:00

abstract：BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

journal_title：Microbial cell factories

authors： Upadhyay V,Singh A,Jha D,Singh A,Panda AK

更新日期：2016-06-08 00:00:00