A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC.

abstract：BACKGROUND:The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics and a work-intense s...

journal_title：Microbial cell factories

authors： Schwarzhans JP,Wibberg D,Winkler A,Luttermann T,Kalinowski J,Friehs K

更新日期：2016-05-20 00:00:00

abstract：INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...

journal_title：Microbial cell factories

authors： Haq SF,Shanbhag AP,Karthikeyan S,Hassan I,Thanukrishnan K,Ashok A,Sukumaran S,Ramaswamy S,Bharatham N,Datta S,Samant S,Katagihallimath N

更新日期：2018-12-03 00:00:00

abstract：BACKGROUND:Strains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination and modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced in the ...

journal_title：Microbial cell factories

authors： Ma L,Li Y,Chen X,Ding M,Wu Y,Yuan YJ

更新日期：2019-03-11 00:00:00

abstract：BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...

journal_title：Microbial cell factories

authors： Lehtinen T,Efimova E,Santala S,Santala V

更新日期：2018-02-08 00:00:00

abstract：:Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fu...

journal_title：Microbial cell factories

authors： Hou X,Shen Z,Li N,Kong X,Sheng K,Wang J,Wang Y

更新日期：2020-04-06 00:00:00

abstract：BACKGROUND:Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors and salts that affect the performance of the microbial production host. The performance of six industr...

journal_title：Microbial cell factories

authors： Rumbold K,van Buijsen HJ,Overkamp KM,van Groenestijn JW,Punt PJ,van der Werf MJ

更新日期：2009-12-04 00:00:00

abstract：BACKGROUND:It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of practical interest for the heterologous protein production, PHB production, etc. Although the carbon and...

journal_title：Microbial cell factories

authors： Kumar R,Shimizu K

更新日期：2010-01-27 00:00:00

abstract：BACKGROUND:The expression of heterologous proteins in Escherichia coli is strongly affected by codon bias. This phenomenon occurs when the codon usage of the mRNA coding for the foreign protein differs from that of the bacterium. The ribosome pauses upon encountering a rare codon and may detach from the mRNA, thereby t...

journal_title：Microbial cell factories

authors： Rosano GL,Ceccarelli EA

更新日期：2009-07-24 00:00:00

abstract：BACKGROUND:Bioethanol produced by the yeast Saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. Lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. However, hemicellulosic materials cannot be...

journal_title：Microbial cell factories

authors： Hasunuma T,Hori Y,Sakamoto T,Ochiai M,Hatanaka H,Kondo A

更新日期：2014-10-12 00:00:00

abstract：BACKGROUND:Bacterial surface display is of interest in many applications, including live vaccine development, screening of protein libraries and the development of whole cell biocatalysts. The goal of this work was to understand which parameters result in production of large quantities of cells that at the same time ex...

journal_title：Microbial cell factories

authors： Gustavsson M,Bäcklund E,Larsson G

更新日期：2011-09-14 00:00:00

abstract：BACKGROUND:We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investiga...

journal_title：Microbial cell factories

authors： Butorac K,Banić M,Novak J,Leboš Pavunc A,Uroić K,Durgo K,Oršolić N,Kukolj M,Radović S,Scalabrin S,Žučko J,Starčević A,Šušković J,Kos B

更新日期：2020-05-19 00:00:00

abstract：BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...

journal_title：Microbial cell factories

authors： Yue YC,Yang BY,Lu J,Zhang SW,Liu L,Nassar K,Xu XX,Pang XY,Lv JP

更新日期：2020-11-23 00:00:00

abstract：BACKGROUND:Protoilludene is a valuable sesquiterpene and serves as a precursor for several medicinal compounds and antimicrobial chemicals. It can be synthesized by heterologous expression of protoilludene synthase in Escherichia coli with overexpression of mevalonate (MVA) or methylerythritol-phosphate (MEP) pathway, ...

journal_title：Microbial cell factories

authors： Yang L,Wang C,Zhou J,Kim SW

更新日期：2016-01-19 00:00:00

abstract：BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...

journal_title：Microbial cell factories

authors： Silva R,Aguiar TQ,Coelho E,Jiménez A,Revuelta JL,Domingues L

更新日期：2019-03-28 00:00:00

abstract：:Recent evidence has demonstrated that cell-to-cell signaling is a fundamental activity carried out by numerous microorganisms. A number of specialized processes are reported to be regulated by density-dependent signaling molecules including antibiotic production, bioluminescence, biofilm formation, genetic competence,...

journal_title：Microbial cell factories

authors： DeLisa MP,Bentley WE

更新日期：2002-12-20 00:00:00

abstract：BACKGROUND:The Bacillus subtilis spore has long been used to display antigens and enzymes. Spore display can be accomplished by a recombinant and a non-recombinant approach, with the latter proved more efficient than the recombinant one. We used the non-recombinant approach to independently adsorb two thermophilic enzy...

journal_title：Microbial cell factories

authors： Mattossovich R,Iacono R,Cangiano G,Cobucci-Ponzano B,Isticato R,Moracci M,Ricca E

更新日期：2017-11-28 00:00:00

abstract：BACKGROUND:Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study...

journal_title：Microbial cell factories

authors： Ferndahl C,Bonander N,Logez C,Wagner R,Gustafsson L,Larsson C,Hedfalk K,Darby RA,Bill RM

更新日期：2010-06-17 00:00:00

abstract：BACKGROUND:Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, constituting direct repeats (D...

journal_title：Microbial cell factories

authors： Ding T,Huang C,Liang Z,Ma X,Wang N,Huo YX

更新日期：2020-03-10 00:00:00

abstract：BACKGROUND:L-tryptophan is an aromatic amino acid widely used in the food, chemical and pharmaceutical industries. In Escherichia coli, L-tryptophan is synthesized from phosphoenolpyruvate and erythrose 4-phosphate by enzymes in the shikimate pathway and L-tryptophan branch pathway, while L-serine and phosphoribosylpyr...

journal_title：Microbial cell factories

authors： Gu P,Yang F,Kang J,Wang Q,Qi Q

更新日期：2012-03-02 00:00:00

abstract：: The aim of this work is to present a critical review on slurry bioreactors (SB) and their application to bioremediation of soils and sediments polluted with recalcitrant and toxic compounds. The scope of the review encompasses the following subjects: (i) process fundamentals of SB and analysis of advantages and disad...

journal_title：Microbial cell factories

authors： Robles-González IV,Fava F,Poggi-Varaldo HM

更新日期：2008-02-29 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, ...

journal_title：Microbial cell factories

authors： Yamaoka M,Osawa S,Morinaga T,Takenaka S,Yoshida K

更新日期：2011-09-07 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：BACKGROUND:A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS:Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. O...

journal_title：Microbial cell factories

authors： Faijes M,Mars AE,Smid EJ

更新日期：2007-08-20 00:00:00

abstract：BACKGROUND:Green microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entiti...

journal_title：Microbial cell factories

authors： Sivakumar G,Jeong K,Lay JO Jr

更新日期：2014-06-03 00:00:00

abstract：BACKGROUND:Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by lignocellulose-degrading fungi including Pycnoporus cinnabarinus. We investigated the cellulolytic system of P. cinnabarinus, focusing on the involvement of CDH in the deconstruction of lignocellulosic biomass. RESULTS:First, P. ...

journal_title：Microbial cell factories

authors： Bey M,Berrin JG,Poidevin L,Sigoillot JC

更新日期：2011-12-28 00:00:00

abstract：BACKGROUND:Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of medical interest, as it is "Generally Recognized As Safe". Furthermore, as L. lactis naturally secretes only one major endoge...

journal_title：Microbial cell factories

authors： Samazan F,Rokbi B,Seguin D,Telles F,Gautier V,Richarme G,Chevret D,Varela PF,Velours C,Poquet I

更新日期：2015-07-16 00:00:00

abstract：BACKGROUND:Cell surface display of recombinant proteins has become a powerful tool for biotechnology and biomedical applications. As a model eukaryotic microorganism, Saccharomyces cerevisiae is an ideal candidate for surface display of heterologous proteins. However, the frequently used commercial yeast surface displa...

journal_title：Microbial cell factories

authors： Yang X,Tang H,Song M,Shen Y,Hou J,Bao X

更新日期：2019-05-18 00:00:00

abstract：BACKGROUND:Calreticulin (CRT) resides in the endoplasmic reticulum (ER) and functions to chaperone proteins, ensuring proper folding, and intracellular Ca(2+) homeostasis. Emerging evidence shows that CRT is a multifunctional protein with significant roles in physiological and pathological processes with presence both ...

journal_title：Microbial cell factories

authors： Čiplys E,Žitkus E,Gold LI,Daubriac J,Pavlides SC,Højrup P,Houen G,Wang WA,Michalak M,Slibinskas R

更新日期：2015-10-15 00:00:00

abstract：BACKGROUND:The understanding of the molecular basis of yeast tolerance to ethanol may guide the design of rational strategies to increase process performance in industrial alcoholic fermentations. A set of 21 genes encoding multidrug transporters from the ATP-Binding Cassette (ABC) Superfamily and Major Facilitator Sup...

journal_title：Microbial cell factories

authors： Teixeira MC,Godinho CP,Cabrito TR,Mira NP,Sá-Correia I

更新日期：2012-07-27 00:00:00

abstract：BACKGROUND:Thymosin α1 (Tα1), a 28-amino acid Nα-acetylated peptide, has a powerful general immunostimulating activity. Although biosynthesis is an attractive means of large-scale manufacture, to date, Tα1 can only be chemosynthesized because of two obstacles to its biosynthesis: the difficulties in expressing small pe...

journal_title：Microbial cell factories

authors： Ren Y,Yao X,Dai H,Li S,Fang H,Chen H,Zhou C

更新日期：2011-04-22 00:00:00