A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC.


BACKGROUND:Spore-forming bacteria of the Bacillus genus are widely used probiotics known to exert their beneficial effects also through the stimulation of the host immune response. The oral delivery of B. toyonensis spores has been shown to improve the immune response to a parenterally administered viral antigen in mice, suggesting that probiotics may increase the efficiency of systemic vaccines. We used the C fragment of the tetanus toxin (TTFC) as a model antigen to evaluate whether a treatment with B. toyonensis spores affected the immune response to a mucosal antigen. RESULTS:Purified TTFC was given to mice by the nasal route either as a free protein or adsorbed to B. subtilis spores, a mucosal vaccine delivery system proved effective with several antigens, including TTFC. Spore adsorption was extremely efficient and TTFC was shown to be exposed on the spore surface. Spore-adsorbed TTFC was more efficient than the free antigen in inducing an immune response and the probiotic treatment improved the response, increasing the production of TTFC-specific secretory immunoglobin A (sIgA) and causing a faster production of serum IgG. The analysis of the induced cytokines indicated that also the cellular immune response was increased by the probiotic treatment. A 16S RNA-based analysis of the gut microbial composition did not show dramatic differences due to the probiotic treatment. However, the abundance of members of the Ruminiclostridium 6 genus was found to correlate with the increased immune response of animals immunized with the spore-adsorbed antigen and treated with the probiotic. CONCLUSION:Our results indicate that B. toyonensis spores significantly contribute to the humoral and cellular responses elicited by a mucosal immunization with spore-adsorbed TTFC, pointing to the probiotic treatment as an alternative to the use of adjuvants for mucosal vaccinations.


Microb Cell Fact


Microbial cell factories


Santos FDS,Mazzoli A,Maia AR,Saggese A,Isticato R,Leite F,Iossa S,Ricca E,Baccigalupi L




Has Abstract


2020-02-19 00:00:00












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    abstract:BACKGROUND:Rhodosporidium and Rhodotorula are two genera of oleaginous red yeast with great potential for industrial biotechnology. To date, there is no effective method for inducible expression of proteins and RNAs in these hosts. RESULTS:We have developed a luciferase gene reporter assay based on a new codon-optimiz...

    journal_title:Microbial cell factories

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    authors: Liu Y,Koh CMJ,Ngoh ST,Ji L

    更新日期:2015-10-26 00:00:00

  • Automated assay for screening the enzymatic release of reducing sugars from micronized biomass.

    abstract:BACKGROUND:To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-thr...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Navarro D,Couturier M,da Silva GG,Berrin JG,Rouau X,Asther M,Bignon C

    更新日期:2010-07-16 00:00:00

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    abstract::Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...

    journal_title:Microbial cell factories

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    authors: Cai G,Zheng W,Yang X,Zhang B,Zheng T

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  • A novel method to prepare L-arabinose from xylose mother liquor by yeast-mediated biopurification.

    abstract:BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Cheng H,Wang H,Lv J,Jiang M,Lin S,Deng Z

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  • Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum.

    abstract:BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Wu S,Gu W,Huang A,Li Y,Kumar M,Lim PE,Huan L,Gao S,Wang G

    更新日期:2019-09-23 00:00:00

  • Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli.

    abstract:BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Menzella HG

    更新日期:2011-03-03 00:00:00

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    pub_type: 杂志文章


    authors: Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

    更新日期:2012-11-04 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Shigemori S,Watanabe T,Kudoh K,Ihara M,Nigar S,Yamamoto Y,Suda Y,Sato T,Kitazawa H,Shimosato T

    更新日期:2015-11-25 00:00:00

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    authors: Antonucci I,Gallo G,Limauro D,Contursi P,Ribeiro AL,Blesa A,Berenguer J,Bartolucci S,Fiorentino G

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    abstract:BACKGROUND:The rational design of L-phenylalanine (L-Phe) overproducing microorganisms has been successfully achieved by combining different genetic strategies such as inactivation of the phosphoenolpyruvate: phosphotransferase transport system (PTS) and overexpression of key genes (DAHP synthase, transketolase and cho...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: D'Urzo N,Martinelli M,Nenci C,Brettoni C,Telford JL,Maione D

    更新日期:2013-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Liu JL,Shriver-Lake LC,Anderson GP,Zabetakis D,Goldman ER

    更新日期:2017-12-12 00:00:00

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    journal_title:Microbial cell factories

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    authors: Cai D,Wang H,He P,Zhu C,Wang Q,Wei X,Nomura CT,Chen S

    更新日期:2017-04-24 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Dietzsch C,Spadiut O,Herwig C

    更新日期:2011-03-03 00:00:00

  • Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD.

    abstract:BACKGROUND:Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complica...

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    pub_type: 杂志文章


    authors: Xue YP,Zeng H,Jin XL,Liu ZQ,Zheng YG

    更新日期:2016-09-22 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Marisch K,Bayer K,Cserjan-Puschmann M,Luchner M,Striedner G

    更新日期:2013-06-11 00:00:00

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    pub_type: 杂志文章,评审


    authors: Liu Q,Yu Z,Tian F,Zhao J,Zhang H,Zhai Q,Chen W

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Yao R,Hirose Y,Sarkar D,Nakahigashi K,Ye Q,Shimizu K

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  • A novel strategy for protein production using non-classical secretion pathway in Bacillus subtilis.

    abstract:BACKGROUND:The Gram-positive bacterium Bacillus subtilis has been widely used as a cell factory for the production of proteins due to its generally regarded as safe (GRAS) nature and secretion capability. Of the known secretory pathways in B. subtilis, the majority of proteins are exported from the cytoplasm by Sec pat...

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    pub_type: 杂志文章


    authors: Chen J,Zhao L,Fu G,Zhou W,Sun Y,Zheng P,Sun J,Zhang D

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    abstract:BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Rohe P,Venkanna D,Kleine B,Freudl R,Oldiges M

    更新日期:2012-10-31 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Hall AE,Engevik MA,Oezguen N,Haag A,Versalovic J

    更新日期:2019-12-12 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Agarwal PK

    更新日期:2006-01-12 00:00:00

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    abstract:BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Yang CH,Liu EJ,Chen YL,Ou-Yang FY,Li SY

    更新日期:2016-08-02 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Jarmander J,Gustavsson M,Do TH,Samuelson P,Larsson G

    更新日期:2012-09-03 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Teixeira MC,Godinho CP,Cabrito TR,Mira NP,Sá-Correia I

    更新日期:2012-07-27 00:00:00

  • Identifying genomic targets for protein over-expression by "omics" analysis of Quiescent Escherichia coli cultures.

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Mahalik S,Sharma AK,Jain P,Mukherjee KJ

    更新日期:2017-07-28 00:00:00

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    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Dong W,Cai Y,Xu Z,Fu B,Chen Q,Cui Y,Ruan Z,Liang Y,Peng N,Zhao S

    更新日期:2020-10-07 00:00:00

  • Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent.

    abstract:BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

    journal_title:Microbial cell factories

    pub_type: 杂志文章


    authors: Upadhyay V,Singh A,Jha D,Singh A,Panda AK

    更新日期:2016-06-08 00:00:00