Regulation of methanol utilisation pathway genes in yeasts.

abstract：BACKGROUND:Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complica...

journal_title：Microbial cell factories

authors： Zhu Y,Hua Y,Zhang B,Sun L,Li W,Kong X,Hong J

更新日期：2017-01-03 00:00:00

abstract：BACKGROUND:In spite of the substantial metabolic engineering effort previously devoted to the development of Saccharomyces cerevisiae strains capable of fermenting both the hexose and pentose sugars present in lignocellulose hydrolysates, the productivity of reported strains for conversion of the naturally most abundan...

journal_title：Microbial cell factories

authors： Krahulec S,Petschacher B,Wallner M,Longus K,Klimacek M,Nidetzky B

更新日期：2010-03-10 00:00:00

abstract：BACKGROUND:The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis. RESULTS:The presence of preformed biomass precursor mol...

journal_title：Microbial cell factories

authors： Li Z,Nimtz M,Rinas U

更新日期：2014-03-23 00:00:00

abstract：BACKGROUND:The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose....

journal_title：Microbial cell factories

authors： Ying H,Tao S,Wang J,Ma W,Chen K,Wang X,Ouyang P

更新日期：2017-03-27 00:00:00

abstract：BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...

journal_title：Microbial cell factories

authors： Hossain AH,Li A,Brickwedde A,Wilms L,Caspers M,Overkamp K,Punt PJ

更新日期：2016-07-28 00:00:00

abstract：BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...

journal_title：Microbial cell factories

authors： Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

更新日期：2015-04-17 00:00:00

abstract：BACKGROUND:Thymosin α1 (Tα1), a 28-amino acid Nα-acetylated peptide, has a powerful general immunostimulating activity. Although biosynthesis is an attractive means of large-scale manufacture, to date, Tα1 can only be chemosynthesized because of two obstacles to its biosynthesis: the difficulties in expressing small pe...

journal_title：Microbial cell factories

authors： Ren Y,Yao X,Dai H,Li S,Fang H,Chen H,Zhou C

更新日期：2011-04-22 00:00:00

abstract：BACKGROUND:The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. Recently, an HPLC-MS-based method to quantify IPTG in the medium and...

journal_title：Microbial cell factories

authors： Fernández-Castané A,Caminal G,López-Santín J

更新日期：2012-05-09 00:00:00

abstract：BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...

journal_title：Microbial cell factories

authors： Nowrouzi B,Li RA,Walls LE,d'Espaux L,Malcı K,Liang L,Jonguitud-Borrego N,Lerma-Escalera AI,Morones-Ramirez JR,Keasling JD,Rios-Solis L

更新日期：2020-11-02 00:00:00

abstract：BACKGROUND:Ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. These compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. Bio...

journal_title：Microbial cell factories

authors： Nielsen CA,Folly C,Hatsch A,Molt A,Schröder H,O'Connor SE,Naesby M

更新日期：2014-08-12 00:00:00

abstract：BACKGROUND:Microalgae has been concerned as a potential source of biodiesel in the recent years. However, it is costly to harvest microalgae as it is commonly cultured in water and the cells are too small to harvest. In order to reduce the cost of cultivation and harvesting, it is important to improve the biomass produ...

journal_title：Microbial cell factories

authors： Chen X,Liu T,Wang Q

更新日期：2014-10-01 00:00:00

abstract：:L-arginine (ARG) is an important amino acid for both medicinal and industrial applications. For almost six decades, the research has been going on for its improved industrial level production using different microorganisms. While the initial approaches involved random mutagenesis for increased tolerance to ARG and con...

journal_title：Microbial cell factories

authors： Shin JH,Lee SY

更新日期：2014-12-03 00:00:00

abstract：:Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features...

journal_title：Microbial cell factories

authors： Song AA,In LLA,Lim SHE,Rahim RA

更新日期：2017-04-04 00:00:00

abstract：BACKGROUND:Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In f...

journal_title：Microbial cell factories

authors： Csörgo B,Fehér T,Tímár E,Blattner FR,Pósfai G

更新日期：2012-01-20 00:00:00

abstract：BACKGROUND:Phasin (PhaP), a kind of polyhydroxyalkanoate (PHA) granule-associated proteins, has a role in controlling the properties of PHA granules surface, and is thought to have influence on PHA biosynthesis in PHA-producing bacteria. This study focused on the phaP1(Re) locus in Ralstonia eutropha as a site of chrom...

journal_title：Microbial cell factories

authors： Kawashima Y,Orita I,Nakamura S,Fukui T

更新日期：2015-11-23 00:00:00

abstract：BACKGROUND:Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation. The aim of this study was the production in high yields of a recombinant pectin lyase that is efficiently s...

journal_title：Microbial cell factories

authors： Papi RM,Chaitidou SA,Trikka FA,Kyriakidis DA

更新日期：2005-12-14 00:00:00

abstract：BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...

journal_title：Microbial cell factories

authors： Claes IJ,Segers ME,Verhoeven TL,Dusselier M,Sels BF,De Keersmaecker SC,Vanderleyden J,Lebeer S

更新日期：2012-12-15 00:00:00

abstract：BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...

journal_title：Microbial cell factories

authors： Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

更新日期：2012-11-04 00:00:00

abstract：BACKGROUND:The efficiency of biotechnological production processes depends on selecting the best performing microbial strain and the optimal cultivation conditions. Thus, many experiments have to be conducted, which conflicts with the demand to speed up drug development processes. Consequently, there is a great need fo...

journal_title：Microbial cell factories

authors： Funke M,Buchenauer A,Mokwa W,Kluge S,Hein L,Müller C,Kensy F,Büchs J

更新日期：2010-11-13 00:00:00

abstract：BACKGROUND:We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investiga...

journal_title：Microbial cell factories

authors： Butorac K,Banić M,Novak J,Leboš Pavunc A,Uroić K,Durgo K,Oršolić N,Kukolj M,Radović S,Scalabrin S,Žučko J,Starčević A,Šušković J,Kos B

更新日期：2020-05-19 00:00:00

abstract：BACKGROUND:Saccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase (PDH) complex ex...

journal_title：Microbial cell factories

authors： Zhang Y,Su M,Qin N,Nielsen J,Liu Z

更新日期：2020-12-10 00:00:00

abstract：BACKGROUND:Messenger RNA decay is an important mechanism for controlling gene expression in all organisms. The rate of the mRNA degradation directly affects the steady state concentration of mRNAs and therefore influences the protein synthesis. RNaseE has a key importance for the general mRNA decay in E. coli. While RN...

journal_title：Microbial cell factories

authors： Kemmer C,Neubauer P

更新日期：2006-12-12 00:00:00

abstract：BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...

journal_title：Microbial cell factories

authors： Rohe P,Venkanna D,Kleine B,Freudl R,Oldiges M

更新日期：2012-10-31 00:00:00

abstract：BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...

journal_title：Microbial cell factories

authors： Otto M,Teixeira PG,Vizcaino MI,David F,Siewers V

更新日期：2019-11-25 00:00:00

abstract：BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...

journal_title：Microbial cell factories

authors： Wons E,Koscielniak D,Szadkowska M,Sektas M

更新日期：2018-09-21 00:00:00

abstract：BACKGROUND:p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmf...

journal_title：Microbial cell factories

authors： Kawai Y,Noda S,Ogino C,Takeshima Y,Okai N,Tanaka T,Kondo A

更新日期：2013-05-07 00:00:00

abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

journal_title：Microbial cell factories

authors： Fei Y,Li N,Zhang DH,Xu JW

更新日期：2019-06-28 00:00:00

abstract：BACKGROUND:Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors and salts that affect the performance of the microbial production host. The performance of six industr...

journal_title：Microbial cell factories

authors： Rumbold K,van Buijsen HJ,Overkamp KM,van Groenestijn JW,Punt PJ,van der Werf MJ

更新日期：2009-12-04 00:00:00

abstract：BACKGROUND:Green microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entiti...

journal_title：Microbial cell factories

authors： Sivakumar G,Jeong K,Lay JO Jr

更新日期：2014-06-03 00:00:00

abstract：BACKGROUND:Emergence of antibiotic resistance and growing consumer trend towards foods containing biopreservatives stimulated the search for alternative antimicrobials. This research is aimed at characterizing, investigating the mechanism of action, scale up optimization and evaluating the biopreservative potential of ...

journal_title：Microbial cell factories

authors： Wayah SB,Philip K

更新日期：2018-08-13 00:00:00