The growth of Scenedesmus sp. attachment on different materials surface.

abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...

journal_title：Microbial cell factories

authors： Wu W,Xing L,Zhou B,Lin Z

更新日期：2011-02-15 00:00:00

abstract：BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...

journal_title：Microbial cell factories

authors： Qiu C,Chen X,Rexida R,Shen Y,Qi Q,Bao X,Hou J

更新日期：2020-07-20 00:00:00

abstract：:The gut microbiota can significantly affect the function of the intestinal barrier. Some intestinal probiotics (such as Lactobacillus, Bifidobacteria, a few Escherichia coli strains, and a new generation of probiotics including Bacteroides thetaiotaomicron and Akkermansia muciniphila) can maintain intestinal epithelia...

journal_title：Microbial cell factories

authors： Liu Q,Yu Z,Tian F,Zhao J,Zhang H,Zhai Q,Chen W

更新日期：2020-02-05 00:00:00

abstract：BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...

journal_title：Microbial cell factories

authors： Bongers M,Chrysanthopoulos PK,Behrendorff JB,Hodson MP,Vickers CE,Nielsen LK

更新日期：2015-11-26 00:00:00

abstract：BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...

journal_title：Microbial cell factories

authors： Bruschi M,Krömer JO,Steen JA,Nielsen LK

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...

journal_title：Microbial cell factories

authors： Yang CH,Liu EJ,Chen YL,Ou-Yang FY,Li SY

更新日期：2016-08-02 00:00:00

abstract：BACKGROUND:Despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. Shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. Thu...

journal_title：Microbial cell factories

authors： Flitsch D,Ladner T,Lukacs M,Büchs J

更新日期：2016-02-24 00:00:00

abstract：BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...

journal_title：Microbial cell factories

authors： Zhang F,Qian X,Si H,Xu G,Han R,Ni Y

更新日期：2015-11-05 00:00:00

abstract：BACKGROUND:β-Ionone is a fragrant terpenoid that generates a pleasant floral scent and is used in diverse applications as a cosmetic and flavoring ingredient. A growing consumer desire for natural products has increased the market demand for natural β-ionone. To date, chemical extraction from plants remains the main ap...

journal_title：Microbial cell factories

authors： Czajka JJ,Nathenson JA,Benites VT,Baidoo EEK,Cheng Q,Wang Y,Tang YJ

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complica...

journal_title：Microbial cell factories

authors： Zhu Y,Hua Y,Zhang B,Sun L,Li W,Kong X,Hong J

更新日期：2017-01-03 00:00:00

abstract：BACKGROUND:Understanding the metabolic mechanism of sterols to produce valuable steroid intermediates in mycobacterium by a noncoding small RNA (sRNA) view is still limited. In the work, RNA-seq was implemented to investigate the noncoding transcriptome of Mycobacterium neoaurum (Mn) in the transformation process of st...

journal_title：Microbial cell factories

authors： Liu M,Zhu ZT,Tao XY,Wang FQ,Wei DZ

更新日期：2016-04-25 00:00:00

abstract：BACKGROUND:A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered ...

journal_title：Microbial cell factories

authors： Liu JL,Shriver-Lake LC,Anderson GP,Zabetakis D,Goldman ER

更新日期：2017-12-12 00:00:00

abstract：BACKGROUND:We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investiga...

journal_title：Microbial cell factories

authors： Butorac K,Banić M,Novak J,Leboš Pavunc A,Uroić K,Durgo K,Oršolić N,Kukolj M,Radović S,Scalabrin S,Žučko J,Starčević A,Šušković J,Kos B

更新日期：2020-05-19 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...

journal_title：Microbial cell factories

authors： Rajamanickam V,Metzger K,Schmid C,Spadiut O

更新日期：2017-09-13 00:00:00

abstract：:A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tiss...

journal_title：Microbial cell factories

authors： Villaverde A,García-Fruitós E,Rinas U,Seras-Franzoso J,Kosoy A,Corchero JL,Vazquez E

更新日期：2012-06-11 00:00:00

abstract：:The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community easy and versatile access to the sequence, two web-sites have been installed as a resource for genomic sequence, gene and protein...

journal_title：Microbial cell factories

authors： Mattanovich D,Callewaert N,Rouzé P,Lin YC,Graf A,Redl A,Tiels P,Gasser B,De Schutter K

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:Although the citric acid fermentation by Aspergillus niger is one of the most important industrial microbial processes and various aspects of the fermentation appear in a very large number of publications since the 1950s, the effect of the spore inoculum level on fungal morphology is a rather neglected area....

journal_title：Microbial cell factories

authors： Papagianni M,Mattey M

更新日期：2006-01-25 00:00:00

abstract：BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...

journal_title：Microbial cell factories

authors： Baritugo KA,Kim HT,David Y,Khang TU,Hyun SM,Kang KH,Yu JH,Choi JH,Song JJ,Joo JC,Park SJ

更新日期：2018-08-21 00:00:00

abstract：BACKGROUND:4-Hydroxycinnamaldehydes are important intermediates in several secondary metabolism pathways, including those involved in the biosynthesis of phenolic acids, flavonoids, terpenoids and monolignols. They are also involved in the biosynthesis and degradation of lignins, which are important limiting factors du...

journal_title：Microbial cell factories

authors： Liu S,Qi Q,Chao N,Hou J,Rao G,Xie J,Lu H,Jiang X,Gai Y

更新日期：2015-08-12 00:00:00

abstract：BACKGROUND:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for ...

journal_title：Microbial cell factories

authors： Lee D,Lloyd ND,Pretorius IS,Borneman AR

更新日期：2016-03-04 00:00:00

abstract：BACKGROUND:The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potenti...

journal_title：Microbial cell factories

authors： Yang YJ,Wang Y,Li ZF,Gong Y,Zhang P,Hu WC,Sheng DH,Li YZ

更新日期：2017-08-16 00:00:00

abstract：BACKGROUND:Extracts and products (roots and/or aerial parts) from Echinacea ssp. represent a profitable market sector for herbal medicines thanks to different functional features. Alkamides and polyacetylenes, phenols like caffeic acid and its derivatives, polysaccharides and glycoproteins are the main bioactive compou...

journal_title：Microbial cell factories

authors： Rizzello CG,Coda R,Macías DS,Pinto D,Marzani B,Filannino P,Giuliani G,Paradiso VM,Di Cagno R,Gobbetti M

更新日期：2013-05-04 00:00:00

abstract：BACKGROUND:Bioethanol produced by the yeast Saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. Lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. However, hemicellulosic materials cannot be...

journal_title：Microbial cell factories

authors： Hasunuma T,Hori Y,Sakamoto T,Ochiai M,Hatanaka H,Kondo A

更新日期：2014-10-12 00:00:00

abstract：BACKGROUND:The Bacillus subtilis spore has long been used to display antigens and enzymes. Spore display can be accomplished by a recombinant and a non-recombinant approach, with the latter proved more efficient than the recombinant one. We used the non-recombinant approach to independently adsorb two thermophilic enzy...

journal_title：Microbial cell factories

authors： Mattossovich R,Iacono R,Cangiano G,Cobucci-Ponzano B,Isticato R,Moracci M,Ricca E

更新日期：2017-11-28 00:00:00

abstract：:Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...

journal_title：Microbial cell factories

authors： Cai G,Zheng W,Yang X,Zhang B,Zheng T

更新日期：2014-05-24 00:00:00

abstract：BACKGROUND:L-tryptophan is an aromatic amino acid widely used in the food, chemical and pharmaceutical industries. In Escherichia coli, L-tryptophan is synthesized from phosphoenolpyruvate and erythrose 4-phosphate by enzymes in the shikimate pathway and L-tryptophan branch pathway, while L-serine and phosphoribosylpyr...

journal_title：Microbial cell factories

authors： Gu P,Yang F,Kang J,Wang Q,Qi Q

更新日期：2012-03-02 00:00:00

abstract：BACKGROUND:Under N-limiting conditions, Saccharomyces cerevisiae strains display a substantial variability in their biomass yield from consumed nitrogen -in particular wine yeasts exhibit high growth abilities- that is correlated with their capacity to complete alcoholic fermentation, a trait of interest for fermented ...

journal_title：Microbial cell factories

authors： Crépin L,Sanchez I,Nidelet T,Dequin S,Camarasa C

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by lignocellulose-degrading fungi including Pycnoporus cinnabarinus. We investigated the cellulolytic system of P. cinnabarinus, focusing on the involvement of CDH in the deconstruction of lignocellulosic biomass. RESULTS:First, P. ...

journal_title：Microbial cell factories

authors： Bey M,Berrin JG,Poidevin L,Sigoillot JC

更新日期：2011-12-28 00:00:00

abstract：BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...

journal_title：Microbial cell factories

authors： Claes IJ,Segers ME,Verhoeven TL,Dusselier M,Sels BF,De Keersmaecker SC,Vanderleyden J,Lebeer S

更新日期：2012-12-15 00:00:00

abstract：BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...

journal_title：Microbial cell factories

authors： Li P,Sun H,Chen Z,Li Y,Zhu T

更新日期：2015-02-21 00:00:00