Open access to sequence: browsing the Pichia pastoris genome.

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：BACKGROUND:Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the maj...

journal_title：Microbial cell factories

authors： Yin H,Zhang R,Xia M,Bai X,Mou J,Zheng Y,Wang M

更新日期：2017-06-15 00:00:00

abstract：BACKGROUND:Calreticulin (CRT) resides in the endoplasmic reticulum (ER) and functions to chaperone proteins, ensuring proper folding, and intracellular Ca(2+) homeostasis. Emerging evidence shows that CRT is a multifunctional protein with significant roles in physiological and pathological processes with presence both ...

journal_title：Microbial cell factories

authors： Čiplys E,Žitkus E,Gold LI,Daubriac J,Pavlides SC,Højrup P,Houen G,Wang WA,Michalak M,Slibinskas R

更新日期：2015-10-15 00:00:00

abstract：:Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fu...

journal_title：Microbial cell factories

authors： Hou X,Shen Z,Li N,Kong X,Sheng K,Wang J,Wang Y

更新日期：2020-04-06 00:00:00

abstract：:A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tiss...

journal_title：Microbial cell factories

authors： Villaverde A,García-Fruitós E,Rinas U,Seras-Franzoso J,Kosoy A,Corchero JL,Vazquez E

更新日期：2012-06-11 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inosi...

journal_title：Microbial cell factories

authors： Tanaka K,Natsume A,Ishikawa S,Takenaka S,Yoshida KI

更新日期：2017-04-21 00:00:00

abstract：BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...

journal_title：Microbial cell factories

authors： Lehtinen T,Efimova E,Santala S,Santala V

更新日期：2018-02-08 00:00:00

abstract：BACKGROUND:Acetic acid is a byproduct of Saccharomyces cerevisiae alcoholic fermentation. Together with high concentrations of ethanol and other toxic metabolites, acetic acid may contribute to fermentation arrest and reduced ethanol productivity. This weak acid is also a present in lignocellulosic hydrolysates, a high...

journal_title：Microbial cell factories

authors： Mira NP,Palma M,Guerreiro JF,Sá-Correia I

更新日期：2010-10-25 00:00:00

abstract：BACKGROUND:Chitinases are ubiquitous enzymes that have gained a recent biotechnological attention due to their ability to transform biological waste from chitin into valued chito-oligomers with wide agricultural, industrial or medical applications. The biological activity of these molecules is related to their size and...

journal_title：Microbial cell factories

authors： Kidibule PE,Santos-Moriano P,Jiménez-Ortega E,Ramírez-Escudero M,Limón MC,Remacha M,Plou FJ,Sanz-Aparicio J,Fernández-Lobato M

更新日期：2018-03-22 00:00:00

abstract：:Lactococcus lactis has progressed a long way since its discovery and initial use in dairy product fermentation, to its present biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites that transcends the heterologous species barrier. Key desirable features...

journal_title：Microbial cell factories

authors： Song AA,In LLA,Lim SHE,Rahim RA

更新日期：2017-04-04 00:00:00

abstract：BACKGROUND:Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermophilus α-amylase in B...

journal_title：Microbial cell factories

authors： Yao D,Su L,Li N,Wu J

更新日期：2019-04-11 00:00:00

abstract：BACKGROUND:Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. RESULTS:In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-produci...

journal_title：Microbial cell factories

authors： Lu H,Liu X,Huang M,Xia J,Chu J,Zhuang Y,Zhang S,Noorman H

更新日期：2015-09-17 00:00:00

abstract：BACKGROUND:Recent years have seen a huge growth in the market of industrial yeasts with the need for strains affording better performance or to be used in new applications. Stress tolerance of commercial Saccharomyces cerevisiae yeasts is, without doubt, a trait that needs improving. Such trait is, however, complex, an...

journal_title：Microbial cell factories

authors： Pérez-Torrado R,Panadero J,Hernández-López MJ,Prieto JA,Randez-Gil F

更新日期：2010-07-13 00:00:00

abstract：BACKGROUND:Cell surface display of recombinant proteins has become a powerful tool for biotechnology and biomedical applications. As a model eukaryotic microorganism, Saccharomyces cerevisiae is an ideal candidate for surface display of heterologous proteins. However, the frequently used commercial yeast surface displa...

journal_title：Microbial cell factories

authors： Yang X,Tang H,Song M,Shen Y,Hou J,Bao X

更新日期：2019-05-18 00:00:00

abstract：BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...

journal_title：Microbial cell factories

authors： Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

更新日期：2012-11-04 00:00:00

abstract：:Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use of strong methanol-...

journal_title：Microbial cell factories

authors： Hartner FS,Glieder A

更新日期：2006-12-14 00:00:00

abstract：BACKGROUND:Predictable control of gene expression is necessary for the rational design and optimization of cell factories. In the yeast Saccharomyces cerevisiae, the promoter is one of the most important tools available for controlling gene expression. However, the complex expression patterns of yeast promoters have no...

journal_title：Microbial cell factories

authors： Peng B,Williams TC,Henry M,Nielsen LK,Vickers CE

更新日期：2015-06-26 00:00:00

abstract：BACKGROUND:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for ...

journal_title：Microbial cell factories

authors： Lee D,Lloyd ND,Pretorius IS,Borneman AR

更新日期：2016-03-04 00:00:00

abstract：BACKGROUND:The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics and a work-intense s...

journal_title：Microbial cell factories

authors： Schwarzhans JP,Wibberg D,Winkler A,Luttermann T,Kalinowski J,Friehs K

更新日期：2016-05-20 00:00:00

abstract：BACKGROUND:Recombinant protein expression in bacteria often leads to the formation of intracellular insoluble protein deposits, a major bottleneck for the production of soluble and active products. However, in recent years, these bacterial protein aggregates, commonly known as inclusion bodies (IBs), have been shown to...

journal_title：Microbial cell factories

authors： Gil-Garcia M,Navarro S,Ventura S

更新日期：2020-06-01 00:00:00

abstract：BACKGROUND:Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis in the filamentous fungal cell factory Aspergillus niger indicated that low a...

journal_title：Microbial cell factories

authors： Sui YF,Schütze T,Ouyang LM,Lu H,Liu P,Xiao X,Qi J,Zhuang YP,Meyer V

更新日期：2020-10-23 00:00:00

abstract：BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...

journal_title：Microbial cell factories

authors： Bruschi M,Krömer JO,Steen JA,Nielsen LK

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...

journal_title：Microbial cell factories

authors： Wu S,Gu W,Huang A,Li Y,Kumar M,Lim PE,Huan L,Gao S,Wang G

更新日期：2019-09-23 00:00:00

abstract：:Industrial development may result in the increase of environmental risks. The enzymatic transformation of polluting compounds to less toxic or even innocuous products is an alternative to their complete removal. In this regard, a number of different redox enzymes are able to transform a wide variety of toxic pollutant...

journal_title：Microbial cell factories

authors： Garcia-Arellano H,Alcalde M,Ballesteros A

更新日期：2004-08-02 00:00:00

abstract：BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...

journal_title：Microbial cell factories

authors： Zhang F,Qian X,Si H,Xu G,Han R,Ni Y

更新日期：2015-11-05 00:00:00

abstract：BACKGROUND:The Gram-positive bacterium Bacillus subtilis has been widely used as a cell factory for the production of proteins due to its generally regarded as safe (GRAS) nature and secretion capability. Of the known secretory pathways in B. subtilis, the majority of proteins are exported from the cytoplasm by Sec pat...

journal_title：Microbial cell factories

authors： Chen J,Zhao L,Fu G,Zhou W,Sun Y,Zheng P,Sun J,Zhang D

更新日期：2016-04-28 00:00:00

abstract：BACKGROUND:Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose r...

journal_title：Microbial cell factories

authors： Cheng H,Wang S,Bilal M,Ge X,Zhang C,Fickers P,Cheng H

更新日期：2018-08-29 00:00:00

abstract：BACKGROUND:A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS:Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. O...

journal_title：Microbial cell factories

authors： Faijes M,Mars AE,Smid EJ

更新日期：2007-08-20 00:00:00

abstract：BACKGROUND:Numerous challenges remain to achieve industrially competitive space-time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development and biocatalyst selection; studies at conventional lab scale are ti...

journal_title：Microbial cell factories

authors： Kolmar JF,Thum O,Baganz F

更新日期：2017-10-10 00:00:00

abstract：BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...

journal_title：Microbial cell factories

authors： Santala S,Efimova E,Kivinen V,Larjo A,Aho T,Karp M,Santala V

更新日期：2011-05-18 00:00:00