Development of novel surface display platforms for anchoring heterologous proteins in Saccharomyces cerevisiae.

abstract：:Innovative new genome engineering technologies for manipulating chromosomes have appeared in the last decade. One of these technologies, recombination mediated genetic engineering (recombineering) allows for precision DNA engineering of chromosomes and plasmids in Escherichia coli. Single-stranded DNA recombineering (...

journal_title：Microbial cell factories

authors： van Pijkeren JP,Britton RA

更新日期：2014-08-29 00:00:00

abstract：BACKGROUND:Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For ...

journal_title：Microbial cell factories

authors： Carrasco M,Alcaíno J,Cifuentes V,Baeza M

更新日期：2017-05-02 00:00:00

abstract：BACKGROUND:Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose r...

journal_title：Microbial cell factories

authors： Cheng H,Wang S,Bilal M,Ge X,Zhang C,Fickers P,Cheng H

更新日期：2018-08-29 00:00:00

abstract：BACKGROUND:In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understood reasons, the produc...

journal_title：Microbial cell factories

authors： Schlegel S,Rujas E,Ytterberg AJ,Zubarev RA,Luirink J,de Gier JW

更新日期：2013-03-12 00:00:00

abstract：BACKGROUND:Gene therapy has gained an increasing interest in its anti-tumor efficiency. However, numerous efforts are required to promote them to clinics. In this study, a novel and efficient delivery platform based on bacterial magnetosomes (BMs) were developed, and the efficiency of BMs in delivering small interferin...

journal_title：Microbial cell factories

authors： Dai Q,Long R,Wang S,Kankala RK,Wang J,Jiang W,Liu Y

更新日期：2017-11-28 00:00:00

abstract：BACKGROUND:The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. Recently, an HPLC-MS-based method to quantify IPTG in the medium and...

journal_title：Microbial cell factories

authors： Fernández-Castané A,Caminal G,López-Santín J

更新日期：2012-05-09 00:00:00

abstract：BACKGROUND:The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-...

journal_title：Microbial cell factories

authors： Huo YY,Jian SL,Cheng H,Rong Z,Cui HL,Xu XW

更新日期：2018-01-30 00:00:00

abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...

journal_title：Microbial cell factories

authors： Wu W,Xing L,Zhou B,Lin Z

更新日期：2011-02-15 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Bot...

journal_title：Microbial cell factories

authors： Rajamanickam V,Metzger K,Schmid C,Spadiut O

更新日期：2017-09-13 00:00:00

abstract：BACKGROUND:The six-carbon circular non-proteinogenic compound L-pipecolic acid is an important chiral drug intermediate with many applications in the pharmaceutical industry. In the present study, we developed a metabolically engineered strain of Escherichia coli for the overproduction of L-pipecolic acid from glucose....

journal_title：Microbial cell factories

authors： Ying H,Tao S,Wang J,Ma W,Chen K,Wang X,Ouyang P

更新日期：2017-03-27 00:00:00

abstract：BACKGROUND:Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database. Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, s...

journal_title：Microbial cell factories

authors： Yang Y,Yang J,Wang R,Liu J,Zhang Y,Liu L,Wang F,Yuan H

更新日期：2019-09-21 00:00:00

abstract：BACKGROUND:Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry to develop new molecules using structure-based drug design studies. Specifically, membrane-bound catechol-O-methyltransferase (MBCOMT) is an...

journal_title：Microbial cell factories

authors： Pedro AQ,Martins LM,Dias JM,Bonifácio MJ,Queiroz JA,Passarinha LA

更新日期：2015-08-07 00:00:00

abstract：BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...

journal_title：Microbial cell factories

authors： Zhang F,Qian X,Si H,Xu G,Han R,Ni Y

更新日期：2015-11-05 00:00:00

abstract：BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...

journal_title：Microbial cell factories

authors： Li P,Sun H,Chen Z,Li Y,Zhu T

更新日期：2015-02-21 00:00:00

abstract：BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...

journal_title：Microbial cell factories

authors： Silva R,Aguiar TQ,Coelho E,Jiménez A,Revuelta JL,Domingues L

更新日期：2019-03-28 00:00:00

abstract：BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...

journal_title：Microbial cell factories

authors： Bongers M,Chrysanthopoulos PK,Behrendorff JB,Hodson MP,Vickers CE,Nielsen LK

更新日期：2015-11-26 00:00:00

abstract：BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...

journal_title：Microbial cell factories

authors： Claes IJ,Segers ME,Verhoeven TL,Dusselier M,Sels BF,De Keersmaecker SC,Vanderleyden J,Lebeer S

更新日期：2012-12-15 00:00:00

abstract：:All of humans and other mammalian species are colonized by some types of microorganisms such as bacteria, archaea, unicellular eukaryotes like fungi and protozoa, multicellular eukaryotes like helminths, and viruses, which in whole are called microbiota. These microorganisms have multiple different types of interactio...

journal_title：Microbial cell factories

authors： Baghbani T,Nikzad H,Azadbakht J,Izadpanah F,Haddad Kashani H

更新日期：2020-11-26 00:00:00

abstract：BACKGROUND:The thermostable serine protease pernisine originates from the hyperthermophilic Archaeaon Aeropyrum pernix and has valuable industrial applications. Due to its properties, A. pernix cannot be cultivated in standard industrial fermentation facilities. Furthermore, pernisine is a demanding target for heterolo...

journal_title：Microbial cell factories

authors： Šnajder M,Carrillo Rincón AF,Magdevska V,Bahun M,Kranjc L,Paš M,Juntes P,Petković H,Poklar Ulrih N

更新日期：2019-11-07 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, ...

journal_title：Microbial cell factories

authors： Yamaoka M,Osawa S,Morinaga T,Takenaka S,Yoshida K

更新日期：2011-09-07 00:00:00

abstract：BACKGROUND:Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. To date, strain specific parameters, which are needed to set up feeding profiles for fed batch cultivations, are determined by time-consuming continuous cultures or consecutive ...

journal_title：Microbial cell factories

authors： Dietzsch C,Spadiut O,Herwig C

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...

journal_title：Microbial cell factories

authors： Scalcinati G,Partow S,Siewers V,Schalk M,Daviet L,Nielsen J

更新日期：2012-08-31 00:00:00

abstract：BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

journal_title：Microbial cell factories

authors： Upadhyay V,Singh A,Jha D,Singh A,Panda AK

更新日期：2016-06-08 00:00:00

abstract：BACKGROUND:Zygosaccharomyces bailii is a diploid budding yeast still poorly characterized, but widely recognised as tolerant to several stresses, most of which related to industrial processes of production. Because of that, it would be very interesting to develop its ability as a cell factory. Gas1p is a beta-1,3-gluca...

journal_title：Microbial cell factories

authors： Passolunghi S,Riboldi L,Dato L,Porro D,Branduardi P

更新日期：2010-01-26 00:00:00

abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

journal_title：Microbial cell factories

authors： Fei Y,Li N,Zhang DH,Xu JW

更新日期：2019-06-28 00:00:00

abstract：BACKGROUND:It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of practical interest for the heterologous protein production, PHB production, etc. Although the carbon and...

journal_title：Microbial cell factories

authors： Kumar R,Shimizu K

更新日期：2010-01-27 00:00:00

abstract：BACKGROUND:Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors and salts that affect the performance of the microbial production host. The performance of six industr...

journal_title：Microbial cell factories

authors： Rumbold K,van Buijsen HJ,Overkamp KM,van Groenestijn JW,Punt PJ,van der Werf MJ

更新日期：2009-12-04 00:00:00

abstract：BACKGROUND:Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. RESULTS:In this stu...

journal_title：Microbial cell factories

authors： Zhang H,Liu Q,Cao Y,Feng X,Zheng Y,Zou H,Liu H,Yang J,Xian M

更新日期：2014-02-10 00:00:00

abstract：BACKGROUND:Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed. To this end, a crucial process is translation initiation which takes place at the ribosome binding s...

journal_title：Microbial cell factories

authors： Volkenborn K,Kuschmierz L,Benz N,Lenz P,Knapp A,Jaeger KE

更新日期：2020-07-29 00:00:00

abstract：BACKGROUND:The classic AOX1 replacement approach is still one of the most often used techniques for expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Although this approach is largely successful, it frequently delivers clones with unpredicted production characteristics and a work-intense s...

journal_title：Microbial cell factories

authors： Schwarzhans JP,Wibberg D,Winkler A,Luttermann T,Kalinowski J,Friehs K

更新日期：2016-05-20 00:00:00