当前位置： SCI文献检索 > Microbial Cell Factories期刊下所有文献 > Coiled-coil inspired functional inclusion bodies.

Coiled-coil inspired functional inclusion bodies.

Abstract：

BACKGROUND:Recombinant protein expression in bacteria often leads to the formation of intracellular insoluble protein deposits, a major bottleneck for the production of soluble and active products. However, in recent years, these bacterial protein aggregates, commonly known as inclusion bodies (IBs), have been shown to be a source of stable and active protein for biotechnological and biomedical applications. The formation of these functional IBs is usually facilitated by the fusion of aggregation-prone peptides or proteins to the protein of interest, leading to the formation of amyloid-like nanostructures, where the functional protein is embedded. RESULTS:In order to offer an alternative to the classical amyloid-like IBs, here we develop functional IBs exploiting the coiled-coil fold. An in silico analysis of coiled-coil and aggregation propensities, net charge, and hydropathicity of different potential tags identified the natural homo-dimeric and anti-parallel coiled-coil ZapB bacterial protein as an optimal candidate to form assemblies in which the native state of the fused protein is preserved. The protein itself forms supramolecular fibrillar networks exhibiting only α-helix secondary structure. This non-amyloid self-assembly propensity allows generating innocuous IBs in which the recombinant protein of interest remains folded and functional, as demonstrated using two different fluorescent proteins. CONCLUSIONS:Here, we present a proof of concept for the use of a natural coiled-coil domain as a versatile tool for the production of functional IBs in bacteria. This α-helix-based strategy excludes any potential toxicity drawback that might arise from the amyloid nature of β-sheet-based IBs and renders highly active and homogeneous submicrometric particles.

journal_name

Microb Cell Fact

journal_title

Microbial cell factories

authors

Gil-Garcia M,Navarro S,Ventura S

doi

10.1186/s12934-020-01375-4

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

117

issue

1

issn

1475-2859

pii

10.1186/s12934-020-01375-4

journal_volume

19

pub_type

杂志文章

相关文献

Microbial Cell Factories文献大全
  • Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli.

    abstract：BACKGROUND:Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a vola...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1080-6

    authors： Duell ER,D'Agostino PM,Shapiro N,Woyke T,Fuchs TM,Gulder TAM

    更新日期：2019-02-07 00:00:00

  • Engineering building blocks for self-assembling protein nanoparticles.

    abstract：:Like natural viruses, manmade protein cages for drug delivery are to be ideally formed by repetitive subunits with self-assembling properties, mimicking viral functions and molecular organization. Naturally formed nanostructures (such as viruses, flagella or simpler protein oligomers) can be engineered to acquire spec...

    journal_title：Microbial cell factories

    pub_type： 社论

    doi：10.1186/1475-2859-9-101

    authors： Vázquez E,Villaverde A

    更新日期：2010-12-30 00:00:00

  • Catabolic regulation analysis of Escherichia coli and its crp, mlc, mgsA, pgi and ptsG mutants.

    abstract：BACKGROUND:Most bacteria can use various compounds as carbon sources. These carbon sources can be either co-metabolized or sequentially metabolized, where the latter phenomenon typically occurs as catabolite repression. From the practical application point of view of utilizing lignocellulose for the production of biofu...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-10-67

    authors： Yao R,Hirose Y,Sarkar D,Nakahigashi K,Ye Q,Shimizu K

    更新日期：2011-08-11 00:00:00

  • Bottleneck in secretion of α-amylase in Bacillus subtilis.

    abstract：:Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In t...

    journal_title：Microbial cell factories

    pub_type： 杂志文章,评审

    doi：10.1186/s12934-017-0738-1

    authors： Yan S,Wu G

    更新日期：2017-07-19 00:00:00

  • Genome-scale metabolic reconstructions and theoretical investigation of methane conversion in Methylomicrobium buryatense strain 5G(B1).

    abstract：BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0377-3

    authors： de la Torre A,Metivier A,Chu F,Laurens LM,Beck DA,Pienkos PT,Lidstrom ME,Kalyuzhnaya MG

    更新日期：2015-11-25 00:00:00

  • Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger.

    abstract：BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-016-0527-2

    authors： Hossain AH,Li A,Brickwedde A,Wilms L,Caspers M,Overkamp K,Punt PJ

    更新日期：2016-07-28 00:00:00

  • Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae.

    abstract：BACKGROUND:Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the mitochondrial thiol oxidase ERV1 gene was the most effective for high GSSG production in Saccharomyc...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0658-0

    authors： Kobayashi J,Sasaki D,Hara KY,Hasunuma T,Kondo A

    更新日期：2017-03-15 00:00:00

  • Mevalonate production from ethanol by direct conversion through acetyl-CoA using recombinant Pseudomonas putida, a novel biocatalyst for terpenoid production.

    abstract：BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-019-1213-y

    authors： Yang J,Son JH,Kim H,Cho S,Na JG,Yeon YJ,Lee J

    更新日期：2019-10-10 00:00:00

  • The growth of Scenedesmus sp. attachment on different materials surface.

    abstract：BACKGROUND:Microalgae has been concerned as a potential source of biodiesel in the recent years. However, it is costly to harvest microalgae as it is commonly cultured in water and the cells are too small to harvest. In order to reduce the cost of cultivation and harvesting, it is important to improve the biomass produ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0142-z

    authors： Chen X,Liu T,Wang Q

    更新日期：2014-10-01 00:00:00

  • Production of the short peptide surfactant DAMP4 from glucose or sucrose in high cell density cultures of Escherichia coli BL21(DE3).

    abstract：BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0099-y

    authors： Bruschi M,Krömer JO,Steen JA,Nielsen LK

    更新日期：2014-08-19 00:00:00

  • Modulation of endogenous pathways enhances bioethanol yield and productivity in Escherichia coli.

    abstract：BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-11-145

    authors： Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

    更新日期：2012-11-04 00:00:00

  • Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium.

    abstract：BACKGROUND:Cholesterol, the precursor of all steroid hormones, is the most abundant steroid in vertebrates and exhibits highly hydrophobic properties, rendering it a difficult substrate for aqueous microbial biotransformations. In the present study, we developed a Bacillus megaterium based whole-cell system that allows...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0300-y

    authors： Gerber A,Kleser M,Biedendieck R,Bernhardt R,Hannemann F

    更新日期：2015-07-29 00:00:00

  • Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite.

    abstract：BACKGROUND:High-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product and is not affected by secondary phenotypes. However, metabolic engineering can result in unintended phenotypes that may go unnoticed in initial screening. The red pigme...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-015-0381-7

    authors： Bongers M,Chrysanthopoulos PK,Behrendorff JB,Hodson MP,Vickers CE,Nielsen LK

    更新日期：2015-11-26 00:00:00

  • A new-generation of Bacillus subtilis cell factory for further elevated scyllo-inositol production.

    abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inosi...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0682-0

    authors： Tanaka K,Natsume A,Ishikawa S,Takenaka S,Yoshida KI

    更新日期：2017-04-21 00:00:00

  • Systematic pathway engineering of Corynebacterium glutamicum S9114 for L-ornithine production.

    abstract：BACKGROUND:L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS:In this study, Corynebacterium gl...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0776-8

    authors： Zhang B,Yu M,Zhou Y,Li Y,Ye BC

    更新日期：2017-09-22 00:00:00

  • Heterologous expression of pikromycin biosynthetic gene cluster using Streptomyces artificial chromosome system.

    abstract：BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0708-7

    authors： Pyeon HR,Nah HJ,Kang SH,Choi SS,Kim ES

    更新日期：2017-05-31 00:00:00

  • Metabolic engineering of microorganisms for the production of L-arginine and its derivatives.

    abstract：:L-arginine (ARG) is an important amino acid for both medicinal and industrial applications. For almost six decades, the research has been going on for its improved industrial level production using different microorganisms. While the initial approaches involved random mutagenesis for increased tolerance to ARG and con...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0166-4

    authors： Shin JH,Lee SY

    更新日期：2014-12-03 00:00:00

  • Production in yeast of pseudotype virus-like particles harboring functionally active antibody fragments neutralizing the cytolytic activity of vaginolysin.

    abstract：BACKGROUND:Recombinant antibodies can be produced in different formats and different expression systems. Single chain variable fragments (scFvs) represent an attractive alternative to full-length antibodies and they can be easily produced in bacteria or yeast. However, the scFvs exhibit monovalent antigen-binding prope...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-10-109

    authors： Pleckaityte M,Zvirbliene A,Sezaite I,Gedvilaite A

    更新日期：2011-12-15 00:00:00

  • Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins.

    abstract：BACKGROUND:A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in order to maintain adequate life-long immune memory: this demands for high production levels and degre...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-12-115

    authors： Piubelli L,Campa M,Temporini C,Binda E,Mangione F,Amicosante M,Terreni M,Marinelli F,Pollegioni L

    更新日期：2013-11-19 00:00:00

  • Genetic manipulation of longevity-related genes as a tool to regulate yeast life span and metabolite production during winemaking.

    abstract：BACKGROUND:Yeast viability and vitality are essential for different industrial processes where the yeast Saccharomyces cerevisiae is used as a biotechnological tool. Therefore, the decline of yeast biological functions during aging may compromise their successful biotechnological use. Life span is controlled by a varie...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-12-1

    authors： Orozco H,Matallana E,Aranda A

    更新日期：2013-01-02 00:00:00

  • Nanotechnology, bionanotechnology and microbial cell factories.

    abstract：:Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...

    journal_title：Microbial cell factories

    pub_type： 社论

    doi：10.1186/1475-2859-9-53

    authors： Villaverde A

    更新日期：2010-07-05 00:00:00

  • Increased salt tolerance in Zymomonas mobilis strain generated by adaptative evolution.

    abstract：BACKGROUND:Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol production. Although Z. mobilis is robustly resilient to certain abiotic stress such as ethanol, ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01406-0

    authors： Fuchino K,Bruheim P

    更新日期：2020-07-20 00:00:00

  • RNA-Seq analysis uncovers non-coding small RNA system of Mycobacterium neoaurum in the metabolism of sterols to accumulate steroid intermediates.

    abstract：BACKGROUND:Understanding the metabolic mechanism of sterols to produce valuable steroid intermediates in mycobacterium by a noncoding small RNA (sRNA) view is still limited. In the work, RNA-seq was implemented to investigate the noncoding transcriptome of Mycobacterium neoaurum (Mn) in the transformation process of st...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-016-0462-2

    authors： Liu M,Zhu ZT,Tao XY,Wang FQ,Wei DZ

    更新日期：2016-04-25 00:00:00

  • Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies.

    abstract：BACKGROUND:Activation of silent biosynthetic gene clusters (BGCs) in marine-derived actinomycete strains is a feasible strategy to discover bioactive natural products. Actinoalloteichus sp. AHMU CJ021, isolated from the seashore, was shown to contain an intact but silent caerulomycin A (CRM A) BGC-cam in its genome. Th...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01418-w

    authors： Xie Y,Chen J,Wang B,Chen T,Chen J,Zhang Y,Liu X,Chen Q

    更新日期：2020-08-06 00:00:00

  • Marinobacter sp. from marine sediments produce highly stable surface-active agents for combatting marine oil spills.

    abstract：BACKGROUND:The application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, ...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-017-0797-3

    authors： Raddadi N,Giacomucci L,Totaro G,Fava F

    更新日期：2017-11-02 00:00:00

  • Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicel

    abstract：BACKGROUND:Bioethanol produced by the yeast Saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. Lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. However, hemicellulosic materials cannot be...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-014-0145-9

    authors： Hasunuma T,Hori Y,Sakamoto T,Ochiai M,Hatanaka H,Kondo A

    更新日期：2014-10-12 00:00:00

  • Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure.

    abstract：BACKGROUND:The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intracellular protein for...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-10-47

    authors： Pfeffer M,Maurer M,Köllensperger G,Hann S,Graf AB,Mattanovich D

    更新日期：2011-06-26 00:00:00

  • Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization.

    abstract：BACKGROUND:Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-018-1023-7

    authors： Faria C,Borges N,Rocha I,Santos H

    更新日期：2018-11-16 00:00:00

  • Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures.

    abstract：BACKGROUND:The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. Recently, an HPLC-MS-based method to quantify IPTG in the medium and...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/1475-2859-11-58

    authors： Fernández-Castané A,Caminal G,López-Santín J

    更新日期：2012-05-09 00:00:00

  • Expressing a cytosolic pyruvate dehydrogenase complex to increase free fatty acid production in Saccharomyces cerevisiae.

    abstract：BACKGROUND:Saccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase (PDH) complex ex...

    journal_title：Microbial cell factories

    pub_type： 杂志文章

    doi：10.1186/s12934-020-01493-z

    authors： Zhang Y,Su M,Qin N,Nielsen J,Liu Z

    更新日期：2020-12-10 00:00:00