Abstract:
BACKGROUND:Emergence of antibiotic resistance and growing consumer trend towards foods containing biopreservatives stimulated the search for alternative antimicrobials. This research is aimed at characterizing, investigating the mechanism of action, scale up optimization and evaluating the biopreservative potential of a bacteriocin from Lactobacillus fermentum. RESULTS:Fermencin SA715 is a novel, broad-spectrum, non-pore-forming and cell wall-associated bacteriocin isolated from L. fermentum GA715 of goat milk origin. A combination of hydrophobic interaction chromatography, solid-phase extraction and reversed-phase HPLC was necessary for purification of the bacteriocin to homogeneity. It has a molecular weight of 1792.537 Da as revealed by MALDI-TOF mass spectrometry. Fermencin SA715 is potent at micromolar concentration, possesses high thermal and pH stability and inactivated by proteolytic enzymes thereby revealing its proteinaceous nature. Biomass accumulation and production of fermencin SA715 was optimum in a newly synthesized growth medium. Fermencin SA715 did not occur in the absence of manganese(II) sulphate. Tween 80, ascorbic acid, sodium citrate and magnesium sulphate enhanced the production of fermencin SA715. Sucrose is the preferred carbon source for growth and bacteriocin production. Sodium chloride concentration higher than 1% suppressed growth and production of fermencin SA715. Optimum bacteriocin production occurred at 37 °C and pH 6-7. Scale up of fermencin SA715 production involved batch fermentation in a bioreactor at a constant pH of 6.5 which resulted in enhanced production. Fermencin SA715 doubled the shelf life and improved the microbiological safety of fresh banana. Bacteriocin application followed by refrigeration tripled the shell life of banana. CONCLUSIONS:This study reveals the huge potential of fermencin SA715 as a future biopreservative for bananas and reveals other interesting characteristics which can be exploited in the preservation of other foods. Furthermore insights on the factors influencing the production of fermencin SA715 have been revealed and optimized condition for its production has been established facilitating future commercial production.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Wayah SB,Philip Kdoi
10.1186/s12934-018-0972-1subject
Has Abstractpub_date
2018-08-13 00:00:00pages
125issue
1issn
1475-2859pii
10.1186/s12934-018-0972-1journal_volume
17pub_type
杂志文章abstract::Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In t...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/s12934-017-0738-1
更新日期:2017-07-19 00:00:00
abstract:BACKGROUND:Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis in the filamentous fungal cell factory Aspergillus niger indicated that low a...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01450-w
更新日期:2020-10-23 00:00:00
abstract:BACKGROUND:Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0446-2
更新日期:2016-03-04 00:00:00
abstract::The success of recombinant protein expression seems unpredictable and even good yields of soluble proteins do not guarantee the correct folding. The search for soluble constructs can be performed by exploiting libraries and speeded up by automation, but these approaches are money and time consuming and the tags used f...
journal_title:Microbial cell factories
pub_type: 社论
doi:10.1186/1475-2859-3-12
更新日期:2004-10-07 00:00:00
abstract:BACKGROUND:Most bacteria can use various compounds as carbon sources. These carbon sources can be either co-metabolized or sequentially metabolized, where the latter phenomenon typically occurs as catabolite repression. From the practical application point of view of utilizing lignocellulose for the production of biofu...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-67
更新日期:2011-08-11 00:00:00
abstract:BACKGROUND:Fumaric acid is a commercially important component of foodstuffs, pharmaceuticals and industrial materials, yet the current methods of production are unsustainable and ecologically destructive. RESULTS:In this study, the fumarate biosynthetic pathway involving reductive reactions of the tricarboxylic acid c...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-24
更新日期:2012-02-15 00:00:00
abstract:BACKGROUND:Oil palm empty fruit bunch (EFB) is a lignocellulosic waste produced in palm oil industry. EFB mainly consists of cellulose, hemicellulose (mainly xylan) and lignin and has a great potential to be reused. Converting EFB to fermentable sugars and value-added chemicals is a much better choice than treating EFB...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0157-5
更新日期:2014-11-11 00:00:00
abstract:BACKGROUND:Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0532-5
更新日期:2016-08-11 00:00:00
abstract:BACKGROUND:Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing attention, but there were no reports of successful implementation. Escherichia coli has been engin...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1166-1
更新日期:2019-07-05 00:00:00
abstract::The biotechnology industry has become a key element in modern societies. Within this industry, the production of recombinant enzymes and biopharmaceutical proteins is of major importance. The global markets for such recombinant proteins are growing rapidly and, accordingly, there is a continuous need for new productio...
journal_title:Microbial cell factories
pub_type: 社论
doi:10.1186/1475-2859-12-3
更新日期:2013-01-14 00:00:00
abstract::Recent evidence has demonstrated that cell-to-cell signaling is a fundamental activity carried out by numerous microorganisms. A number of specialized processes are reported to be regulated by density-dependent signaling molecules including antibiotic production, bioluminescence, biofilm formation, genetic competence,...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-1-5
更新日期:2002-12-20 00:00:00
abstract:BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0099-y
更新日期:2014-08-19 00:00:00
abstract:BACKGROUND:Spore-forming bacteria of the Bacillus genus are widely used probiotics known to exert their beneficial effects also through the stimulation of the host immune response. The oral delivery of B. toyonensis spores has been shown to improve the immune response to a parenterally administered viral antigen in mic...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01308-1
更新日期:2020-02-19 00:00:00
abstract:BACKGROUND:Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. RESULTS:In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-produci...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0329-y
更新日期:2015-09-17 00:00:00
abstract:BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-36
更新日期:2011-05-18 00:00:00
abstract:BACKGROUND:Although the citric acid fermentation by Aspergillus niger is one of the most important industrial microbial processes and various aspects of the fermentation appear in a very large number of publications since the 1950s, the effect of the spore inoculum level on fungal morphology is a rather neglected area....
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-5-3
更新日期:2006-01-25 00:00:00
abstract:BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01405-1
更新日期:2020-07-20 00:00:00
abstract::Innovative new genome engineering technologies for manipulating chromosomes have appeared in the last decade. One of these technologies, recombination mediated genetic engineering (recombineering) allows for precision DNA engineering of chromosomes and plasmids in Escherichia coli. Single-stranded DNA recombineering (...
journal_title:Microbial cell factories
pub_type: 杂志文章,评审
doi:10.1186/1475-2859-13-S1-S10
更新日期:2014-08-29 00:00:00
abstract:BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0111-6
更新日期:2014-08-19 00:00:00
abstract:BACKGROUND:Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose r...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0982-z
更新日期:2018-08-29 00:00:00
abstract:BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0560-1
更新日期:2016-09-22 00:00:00
abstract:BACKGROUND:The expression of heterologous proteins in Escherichia coli is strongly affected by codon bias. This phenomenon occurs when the codon usage of the mRNA coding for the foreign protein differs from that of the bacterium. The ribosome pauses upon encountering a rare codon and may detach from the mRNA, thereby t...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-8-41
更新日期:2009-07-24 00:00:00
abstract:BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1032-6
更新日期:2018-11-24 00:00:00
abstract::Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...
journal_title:Microbial cell factories
pub_type: 社论
doi:10.1186/1475-2859-9-53
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:The thermostable serine protease pernisine originates from the hyperthermophilic Archaeaon Aeropyrum pernix and has valuable industrial applications. Due to its properties, A. pernix cannot be cultivated in standard industrial fermentation facilities. Furthermore, pernisine is a demanding target for heterolo...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1245-3
更新日期:2019-11-07 00:00:00
abstract:BACKGROUND:Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis and hepatocellular carcinoma. Current therapies to treat HCV infection are shown to be partially effective and poorly tolerated. Therefore, ample efforts are underway to rationally design therapies targeting the HCV non-structural prot...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-111
更新日期:2013-11-16 00:00:00
abstract:BACKGROUND:The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H:gm. The SefA pro...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-118
更新日期:2012-09-03 00:00:00
abstract:BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01458-2
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:Lipases (EC 3.1.1.3) catalyze the hydrolysis of triacyl glycerol to glycerol and are involved in the synthesis of both short chain and long chain acylglycerols. They are widely used industrially in various applications, such as baking, laundry detergents and as biocatalysts in alternative energy strategies. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-72
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-9
更新日期:2011-02-15 00:00:00