Abstract:
:Recent evidence has demonstrated that cell-to-cell signaling is a fundamental activity carried out by numerous microorganisms. A number of specialized processes are reported to be regulated by density-dependent signaling molecules including antibiotic production, bioluminescence, biofilm formation, genetic competence, sporulation, swarming motility and virulence. However, a more centralized role for quorum sensing is emerging where quorum signaling pathways overlap with stress and starvation circuits to regulate cellular adaptation to changing environmental conditions. The interplay of these phenomena is especially critical in the expression of recombinant proteins where elicitation of stress responses can dramatically impact cellular productivity.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
DeLisa MP,Bentley WEdoi
10.1186/1475-2859-1-5keywords:
subject
Has Abstractpub_date
2002-12-20 00:00:00pages
5issue
1issn
1475-2859journal_volume
1pub_type
杂志文章abstract:BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-36
更新日期:2011-05-18 00:00:00
abstract::Industrial development may result in the increase of environmental risks. The enzymatic transformation of polluting compounds to less toxic or even innocuous products is an alternative to their complete removal. In this regard, a number of different redox enzymes are able to transform a wide variety of toxic pollutant...
journal_title:Microbial cell factories
pub_type: 社论
doi:10.1186/1475-2859-3-10
更新日期:2004-08-02 00:00:00
abstract:BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-161
更新日期:2012-12-15 00:00:00
abstract:BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0869-z
更新日期:2018-02-08 00:00:00
abstract:BACKGROUND:The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potenti...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0758-x
更新日期:2017-08-16 00:00:00
abstract:BACKGROUND:Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutu...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1214-x
更新日期:2019-09-23 00:00:00
abstract:BACKGROUND:Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0936-5
更新日期:2018-06-07 00:00:00
abstract:BACKGROUND:Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages, which is why current ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0292-7
更新日期:2015-07-10 00:00:00
abstract:BACKGROUND:Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metaboli...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01466-2
更新日期:2020-11-23 00:00:00
abstract:BACKGROUND:In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration (FDA)-approved for human applications. Widespread use of E. coli in ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-12-58
更新日期:2013-06-11 00:00:00
abstract:BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0560-1
更新日期:2016-09-22 00:00:00
abstract:BACKGROUND:Lipases (EC 3.1.1.3) catalyze the hydrolysis of triacyl glycerol to glycerol and are involved in the synthesis of both short chain and long chain acylglycerols. They are widely used industrially in various applications, such as baking, laundry detergents and as biocatalysts in alternative energy strategies. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-72
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Messenger RNA decay is an important mechanism for controlling gene expression in all organisms. The rate of the mRNA degradation directly affects the steady state concentration of mRNAs and therefore influences the protein synthesis. RNaseE has a key importance for the general mRNA decay in E. coli. While RN...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-5-38
更新日期:2006-12-12 00:00:00
abstract:BACKGROUND:Bacterial surface display is of interest in many applications, including live vaccine development, screening of protein libraries and the development of whole cell biocatalysts. The goal of this work was to understand which parameters result in production of large quantities of cells that at the same time ex...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-72
更新日期:2011-09-14 00:00:00
abstract:BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0111-6
更新日期:2014-08-19 00:00:00
abstract:BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-13-11
更新日期:2014-01-17 00:00:00
abstract:BACKGROUND:Targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the N-terminus of a secretory protein. These signal sequences usually contain an N-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signa...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0203-y
更新日期:2015-02-14 00:00:00
abstract:BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01405-1
更新日期:2020-07-20 00:00:00
abstract:BACKGROUND:Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the maj...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0717-6
更新日期:2017-06-15 00:00:00
abstract:BACKGROUND:Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a spec...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1113-1
更新日期:2019-03-28 00:00:00
abstract:BACKGROUND:Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their superior physical and mechanical properties. We engineered a reversed fatty acid β-oxidation pathway i...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1186-x
更新日期:2019-08-13 00:00:00
abstract:: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been th...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-7-11
更新日期:2008-04-04 00:00:00
abstract:BACKGROUND:Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. RESULTS:In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-produci...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0329-y
更新日期:2015-09-17 00:00:00
abstract:BACKGROUND:Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis in the filamentous fungal cell factory Aspergillus niger indicated that low a...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01450-w
更新日期:2020-10-23 00:00:00
abstract:BACKGROUND:Oil palm empty fruit bunch (EFB) is a lignocellulosic waste produced in palm oil industry. EFB mainly consists of cellulose, hemicellulose (mainly xylan) and lignin and has a great potential to be reused. Converting EFB to fermentable sugars and value-added chemicals is a much better choice than treating EFB...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0157-5
更新日期:2014-11-11 00:00:00
abstract:BACKGROUND:The Gram-positive bacterium Bacillus subtilis has been widely used as a cell factory for the production of proteins due to its generally regarded as safe (GRAS) nature and secretion capability. Of the known secretory pathways in B. subtilis, the majority of proteins are exported from the cytoplasm by Sec pat...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0469-8
更新日期:2016-04-28 00:00:00
abstract:BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-117
更新日期:2012-08-31 00:00:00
abstract:BACKGROUND:Strains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination and modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced in the ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1102-4
更新日期:2019-03-11 00:00:00
abstract:BACKGROUND:Ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. These compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. Bio...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0095-2
更新日期:2014-08-12 00:00:00
abstract:BACKGROUND:Griseoviridin (GV) and viridogrisein (VG, also referred as etamycin), both biosynthesized by a distinct 105 kb biosynthetic gene cluster (BGC) in Streptomyces griseoviridis NRRL 2427, are a pair of synergistic streptogramin antibiotics and very important in treating infections of many multi-drug resistant mi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0792-8
更新日期:2017-10-25 00:00:00