Abstract：
BACKGROUND:Bacterial surface display is of interest in many applications, including live vaccine development, screening of protein libraries and the development of whole cell biocatalysts. The goal of this work was to understand which parameters result in production of large quantities of cells that at the same time express desired levels of the chosen protein on the cell surface. For this purpose, staphylococcal protein Z was expressed using the AIDA autotransporter in Escherichia coli. RESULTS:The use of an OmpT-negative E. coli mutant resulted in successful expression of the protein on the surface, while a clear degradation pattern was found in the wild type. The expression in the mutant resulted also in a more narrow distribution of the surface-anchored protein within the population. Medium optimisation showed that minimal medium with glucose gave more than four times as high expression as LB-medium. Glucose limited fed-batch was used to increase the cell productivity and the highest protein levels were found at the highest feed rates. A maintained high surface expression up to cell dry weights of 18 g l-1 could also be achieved by repeated glucose additions in batch cultivation where production was eventually reduced by low oxygen levels. In spite of this, the distribution in the bacterial population of the surface protein was narrower using the batch technique. CONCLUSIONS:A number of parameters in recombinant protein production were seen to influence the surface expression of the model protein with respect both to the productivity and to the display on the individual cell. The choice of medium and the cell design to remove proteolytic cleavage were however the most important. Both fed-batch and batch processing can be successfully used, but prolonged batch processing is probably only possible if the chosen strain has a low acetic acid production.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Gustavsson M,Bäcklund E,Larsson Gdoi
10.1186/1475-2859-10-72subject
Has Abstractpub_date
2011-09-14 00:00:00pages
72issn
1475-2859pii
1475-2859-10-72journal_volume
10pub_type
杂志文章abstract：BACKGROUND:Chinese Nong-flavor (NF) liquor is continuously and stably produced by solid-state fermentation technology for 1000 years, resulting in enrichment of special microbial community and enzymes system in its starter. Based on traditional culture-dependent methods, these functional enzymes are hardly obtained. Ac...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0878-y
更新日期：2018-02-22 00:00:00
abstract：BACKGROUND:Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a vola...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-019-1080-6
更新日期：2019-02-07 00:00:00
abstract：BACKGROUND:Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry to develop new molecules using structure-based drug design studies. Specifically, membrane-bound catechol-O-methyltransferase (MBCOMT) is an...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0304-7
更新日期：2015-08-07 00:00:00
abstract：:Dunaliella salina is a unicellular green alga with a high α-linolenic acid (ALA) level, but a low eicosapentaenoic acid (EPA) level. In a previous analysis of the catalytic activity of delta 6 fatty acid desaturase (FADS6) from various species, FADS6 from Thalassiosira pseudonana (TpFADS6), a marine diatom, showed the...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0857-3
更新日期：2018-01-13 00:00:00
abstract：BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0206-8
更新日期：2015-02-21 00:00:00
abstract：BACKGROUND:Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complica...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0620-6
更新日期：2017-01-03 00:00:00
abstract：BACKGROUND:L-Alanyl-L-glutamine (AQ) is a functional dipeptide with high water solubility, good thermal stability and high bioavailability. It is widely used in clinical treatment, post-operative rehabilitation, sports health care and other fields. AQ is mainly produced via chemical synthesis which is complicated, time...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01369-2
更新日期：2020-06-11 00:00:00
abstract：BACKGROUND:Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages, which is why current ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0292-7
更新日期：2015-07-10 00:00:00
abstract：BACKGROUND:Zygosaccharomyces bailii is a diploid budding yeast still poorly characterized, but widely recognised as tolerant to several stresses, most of which related to industrial processes of production. Because of that, it would be very interesting to develop its ability as a cell factory. Gas1p is a beta-1,3-gluca...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-9-7
更新日期：2010-01-26 00:00:00
abstract：BACKGROUND:D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0822-6
更新日期：2017-11-21 00:00:00
abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inosi...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0682-0
更新日期：2017-04-21 00:00:00
abstract：BACKGROUND:Bacillus subtilis is an important industrial workhorse applied in the production of many different commercially relevant proteins, especially enzymes. Virtually all of these proteins are secreted via the general secretion (Sec) pathway. Studies from different laboratories have demonstrated essential or non-e...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01315-2
更新日期：2020-02-28 00:00:00
abstract：BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-13-11
更新日期：2014-01-17 00:00:00
abstract：BACKGROUND:L-tryptophan is an aromatic amino acid widely used in the food, chemical and pharmaceutical industries. In Escherichia coli, L-tryptophan is synthesized from phosphoenolpyruvate and erythrose 4-phosphate by enzymes in the shikimate pathway and L-tryptophan branch pathway, while L-serine and phosphoribosylpyr...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-30
更新日期：2012-03-02 00:00:00
abstract：BACKGROUND:A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in order to maintain adequate life-long immune memory: this demands for high production levels and degre...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-12-115
更新日期：2013-11-19 00:00:00
abstract：BACKGROUND:Fine-tuning the aeration for cultivations when oxygen-limited conditions are demanded (such as the production of vaccines, isobutanol, 2-3 butanediol, acetone, and bioethanol) is still a challenge in the area of bioreactor automation and advanced control. In this work, an innovative control strategy based on...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-019-1198-6
更新日期：2019-09-04 00:00:00
abstract：BACKGROUND:Cholesterol, the precursor of all steroid hormones, is the most abundant steroid in vertebrates and exhibits highly hydrophobic properties, rendering it a difficult substrate for aqueous microbial biotransformations. In the present study, we developed a Bacillus megaterium based whole-cell system that allows...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0300-y
更新日期：2015-07-29 00:00:00
abstract：BACKGROUND:Under aerobic conditions, acetic acid is the major byproduct produced by E. coli during the fermentation. And acetic acid is detrimental to cell growth as it destroys transmembrane pH gradients. Hence, how to reduce the production of acetic acid and how to utilize it as a feedstock are of intriguing interest...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0475-x
更新日期：2016-05-05 00:00:00
abstract：BACKGROUND:Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In f...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-11-11
更新日期：2012-01-20 00:00:00
abstract：BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-015-0368-4
更新日期：2015-11-05 00:00:00
abstract：BACKGROUND:Oxidized glutathione (GSSG) is the preferred form for industrial mass production of glutathione due to its high stability compared with reduced glutathione (GSH). In our previous study, over-expression of the mitochondrial thiol oxidase ERV1 gene was the most effective for high GSSG production in Saccharomyc...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-017-0658-0
更新日期：2017-03-15 00:00:00
abstract：BACKGROUND:Transcriptional engineering has presented a strong ability of phenotypic improvement in microorganisms. However, it could not be directly applied to Actinoplanes teichomyceticus L-27 because of the paucity of endogenous transcription factors in the strain. In this study, exogenous transcription factors were ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-13-10
更新日期：2014-01-16 00:00:00
abstract：:Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use of strong methanol-...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-5-39
更新日期：2006-12-14 00:00:00
abstract：BACKGROUND:The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO2 for biotechnological applications, the genetic toolbox for Synechocystis 6803 is still a limited factor. RESULTS:We systematical...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0897-8
更新日期：2018-03-26 00:00:00
abstract：BACKGROUND:Cinnamyl alcohol is not only a kind of flavoring agent and fragrance, but also a versatile chemical applied in the production of various compounds. At present, the preparation of cinnamyl alcohol depends on plant extraction and chemical synthesis, which have several drawbacks, including limited scalability, ...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-020-01419-9
更新日期：2020-08-12 00:00:00
abstract：BACKGROUND:Catalase (EC 1.11.1.6) is one of the important industrial enzyme employed in diagnostic and analytical methods in the form of biomarkers and biosensors in addition to their enormous applications in textile, paper, food and pharmaceutical sectors. The present study demonstrates the utility of a newly isolated...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-016-0410-1
更新日期：2016-01-14 00:00:00
abstract：BACKGROUND:The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intracellular protein for...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-10-47
更新日期：2011-06-26 00:00:00
abstract：BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-014-0111-6
更新日期：2014-08-19 00:00:00
abstract：BACKGROUND:To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-thr...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/1475-2859-9-58
更新日期：2010-07-16 00:00:00
abstract：BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...
journal_title：Microbial cell factories
pub_type： 杂志文章
doi：10.1186/s12934-018-0977-9
更新日期：2018-08-21 00:00:00
