Abstract:
BACKGROUND:Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. RESULTS:In this study, sabinene was significantly produced by assembling a biosynthetic pathway using the methylerythritol 4-phosphate (MEP) or heterologous mevalonate (MVA) pathway combining the GPP and sabinene synthase genes in an engineered Escherichia coli strain. Subsequently, the culture medium and process conditions were optimized to enhance sabinene production with a maximum titer of 82.18 mg/L. Finally, the fed-batch fermentation of sabinene was evaluated using the optimized culture medium and process conditions, which reached a maximum concentration of 2.65 g/L with an average productivity of 0.018 g h⁻¹ g⁻¹ dry cells, and the conversion efficiency of glycerol to sabinene (gram to gram) reached 3.49%. CONCLUSIONS:This is the first report of microbial synthesis of sabinene using an engineered E. coli strain with the renewable carbon source as feedstock. Therefore, a green and sustainable production strategy has been established for sabinene.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Zhang H,Liu Q,Cao Y,Feng X,Zheng Y,Zou H,Liu H,Yang J,Xian Mdoi
10.1186/1475-2859-13-20subject
Has Abstractpub_date
2014-02-10 00:00:00pages
20issn
1475-2859pii
1475-2859-13-20journal_volume
13pub_type
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