Combinatory optimization of chromosomal integrated mevalonate pathway for β-carotene production in Escherichia coli.

abstract：BACKGROUND:Xylanases randomly cleave the internal β-1,4-glycosidic bonds in the xylan backbone and are grouped into different families in the carbohydrate-active enzyme (CAZy) database. Although multiple xylanases are detected in single strains of many filamentous fungi, no study has been reported on the composition, s...

journal_title：Microbial cell factories

authors： Yang Y,Yang J,Wang R,Liu J,Zhang Y,Liu L,Wang F,Yuan H

更新日期：2019-09-21 00:00:00

abstract：BACKGROUND:The metabolic capabilities of acetogens to ferment a wide range of sugars, to grow autotrophically on H2/CO2, and more importantly on synthesis gas (H2/CO/CO2) make them very attractive candidates as production hosts for biofuels and biocommodities. Acetogenic metabolism is considered one of the earliest mod...

journal_title：Microbial cell factories

authors： Nagarajan H,Sahin M,Nogales J,Latif H,Lovley DR,Ebrahim A,Zengler K

更新日期：2013-11-25 00:00:00

abstract：BACKGROUND:Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complica...

journal_title：Microbial cell factories

authors： Zhu Y,Hua Y,Zhang B,Sun L,Li W,Kong X,Hong J

更新日期：2017-01-03 00:00:00

abstract：BACKGROUND:To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-thr...

journal_title：Microbial cell factories

authors： Navarro D,Couturier M,da Silva GG,Berrin JG,Rouau X,Asther M,Bignon C

更新日期：2010-07-16 00:00:00

abstract：:Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fu...

journal_title：Microbial cell factories

authors： Hou X,Shen Z,Li N,Kong X,Sheng K,Wang J,Wang Y

更新日期：2020-04-06 00:00:00

abstract：BACKGROUND:In our previous study, the feasibility of Rubisco-based engineered E. coli (that contains heterologous phosphoribulokinase (PrkA) and Rubisco) for in situ CO2 recycling during the fermentation of pentoses or hexoses was demonstrated. Nevertheless, it is perplexing to see that only roughly 70 % of the carbon ...

journal_title：Microbial cell factories

authors： Yang CH,Liu EJ,Chen YL,Ou-Yang FY,Li SY

更新日期：2016-08-02 00:00:00

abstract：BACKGROUND:Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol production. Although Z. mobilis is robustly resilient to certain abiotic stress such as ethanol, ...

journal_title：Microbial cell factories

authors： Fuchino K,Bruheim P

更新日期：2020-07-20 00:00:00

abstract：BACKGROUND:Microalgae has been concerned as a potential source of biodiesel in the recent years. However, it is costly to harvest microalgae as it is commonly cultured in water and the cells are too small to harvest. In order to reduce the cost of cultivation and harvesting, it is important to improve the biomass produ...

journal_title：Microbial cell factories

authors： Chen X,Liu T,Wang Q

更新日期：2014-10-01 00:00:00

abstract：BACKGROUND:Numerous challenges remain to achieve industrially competitive space-time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development and biocatalyst selection; studies at conventional lab scale are ti...

journal_title：Microbial cell factories

authors： Kolmar JF,Thum O,Baganz F

更新日期：2017-10-10 00:00:00

abstract：BACKGROUND:For the cultivation of Escherichia coli in bioreactors trace element solutions are generally designed for optimal growth under aerobic conditions. They do normally not contain selenium and nickel. Molybdenum is only contained in few of them. These elements are part of the formate hydrogen lyase (FHL) complex...

journal_title：Microbial cell factories

authors： Soini J,Ukkonen K,Neubauer P

更新日期：2008-08-07 00:00:00

abstract：BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...

journal_title：Microbial cell factories

authors： Otto M,Teixeira PG,Vizcaino MI,David F,Siewers V

更新日期：2019-11-25 00:00:00

abstract：BACKGROUND:Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages, which is why current ...

journal_title：Microbial cell factories

authors： Capone S,Horvat J,Herwig C,Spadiut O

更新日期：2015-07-10 00:00:00

abstract：BACKGROUND:Messenger RNA decay is an important mechanism for controlling gene expression in all organisms. The rate of the mRNA degradation directly affects the steady state concentration of mRNAs and therefore influences the protein synthesis. RNaseE has a key importance for the general mRNA decay in E. coli. While RN...

journal_title：Microbial cell factories

authors： Kemmer C,Neubauer P

更新日期：2006-12-12 00:00:00

abstract：BACKGROUND:A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in order to maintain adequate life-long immune memory: this demands for high production levels and degre...

journal_title：Microbial cell factories

authors： Piubelli L,Campa M,Temporini C,Binda E,Mangione F,Amicosante M,Terreni M,Marinelli F,Pollegioni L

更新日期：2013-11-19 00:00:00

abstract：BACKGROUND:Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study...

journal_title：Microbial cell factories

authors： Ferndahl C,Bonander N,Logez C,Wagner R,Gustafsson L,Larsson C,Hedfalk K,Darby RA,Bill RM

更新日期：2010-06-17 00:00:00

abstract：BACKGROUND:Nicotinamide adenine dinucleotide phosphate (NADPH) is an important cofactor ensuring intracellular redox balance, anabolism and cell growth in all living systems. Our recent multi-omics analyses of glucoamylase (GlaA) biosynthesis in the filamentous fungal cell factory Aspergillus niger indicated that low a...

journal_title：Microbial cell factories

authors： Sui YF,Schütze T,Ouyang LM,Lu H,Liu P,Xiao X,Qi J,Zhuang YP,Meyer V

更新日期：2020-10-23 00:00:00

abstract：BACKGROUND:Trypsin has many applications in food and pharmaceutical manufacturing. Although commercial trypsin is usually extracted from porcine pancreas, this source carries the risks of infectivity and immunogenicity. Microbial Streptomyces griseus trypsin (SGT) is a prime alternative because it possesses efficient h...

journal_title：Microbial cell factories

authors： Zhang Y,Liang Q,Zhang C,Zhang J,Du G,Kang Z

更新日期：2020-04-13 00:00:00

abstract：BACKGROUND:The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-...

journal_title：Microbial cell factories

authors： Huo YY,Jian SL,Cheng H,Rong Z,Cui HL,Xu XW

更新日期：2018-01-30 00:00:00

abstract：:Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In t...

journal_title：Microbial cell factories

authors： Yan S,Wu G

更新日期：2017-07-19 00:00:00

abstract：BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...

journal_title：Microbial cell factories

authors： Claes IJ,Segers ME,Verhoeven TL,Dusselier M,Sels BF,De Keersmaecker SC,Vanderleyden J,Lebeer S

更新日期：2012-12-15 00:00:00

abstract：BACKGROUND:Corynebacterium glutamicum is a well-known producer of various L-amino acids in industry. During the fermenting process, C. glutamicum unavoidably encounters oxidative stress due to a specific reactive oxygen species (ROS) produced by consistent adverse conditions. To combat the ROS, C. glutamicum has develo...

journal_title：Microbial cell factories

authors： Si M,Su T,Chen C,Liu J,Gong Z,Che C,Li G,Yang G

更新日期：2018-12-26 00:00:00

abstract：BACKGROUND:The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. Recently, an HPLC-MS-based method to quantify IPTG in the medium and...

journal_title：Microbial cell factories

authors： Fernández-Castané A,Caminal G,López-Santín J

更新日期：2012-05-09 00:00:00

abstract：BACKGROUND:Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH buffers, detergents and ...

journal_title：Microbial cell factories

authors： Upadhyay V,Singh A,Jha D,Singh A,Panda AK

更新日期：2016-06-08 00:00:00

abstract：BACKGROUND:Nowadays, microbial infections have caused increasing economic losses in aquaculture industry and deteriorated worldwide environments. Many of these infections are caused by opportunistic pathogens through cell-density mediated quorum sensing (QS). The disruption of QS, known as quorum quenching (QQ), is an ...

journal_title：Microbial cell factories

authors： Dong W,Cai Y,Xu Z,Fu B,Chen Q,Cui Y,Ruan Z,Liang Y,Peng N,Zhao S

更新日期：2020-10-07 00:00:00

abstract：BACKGROUND:Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications deriv...

journal_title：Microbial cell factories

authors： Sun H,Zhao D,Xiong B,Zhang C,Bi C

更新日期：2016-08-24 00:00:00

abstract：BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...

journal_title：Microbial cell factories

authors： Rohe P,Venkanna D,Kleine B,Freudl R,Oldiges M

更新日期：2012-10-31 00:00:00

abstract：BACKGROUND:Anaerobic digesters become unstable when operated at a high organi c loading rate (OLR). Investigating the microbial community response to OLR disturbance is helpful for achieving efficient and stable process operation. However, previous studies have only focused on community succession during different proc...

journal_title：Microbial cell factories

authors： Li L,He Q,Ma Y,Wang X,Peng X

更新日期：2016-04-25 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol (SI), has been regarded as a promising therapeutic agent for Alzheimer's disease. However, this compound is relatively rare, whereas another stereoisomer of inositol, myo-inositol (MI) is abundant in nature. Bacillus subtilis 168 has the ability to metabolize inosi...

journal_title：Microbial cell factories

authors： Tanaka K,Natsume A,Ishikawa S,Takenaka S,Yoshida KI

更新日期：2017-04-21 00:00:00

abstract：BACKGROUND:The understanding of the molecular basis of yeast tolerance to ethanol may guide the design of rational strategies to increase process performance in industrial alcoholic fermentations. A set of 21 genes encoding multidrug transporters from the ATP-Binding Cassette (ABC) Superfamily and Major Facilitator Sup...

journal_title：Microbial cell factories

authors： Teixeira MC,Godinho CP,Cabrito TR,Mira NP,Sá-Correia I

更新日期：2012-07-27 00:00:00

abstract：BACKGROUND:Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accur...

journal_title：Microbial cell factories

authors： de la Torre A,Metivier A,Chu F,Laurens LM,Beck DA,Pienkos PT,Lidstrom ME,Kalyuzhnaya MG

更新日期：2015-11-25 00:00:00