Abstract:
BACKGROUND:The deep-sea environment harbors a vast pool of novel enzymes. Owing to the limitations of cultivation, cultivation-independent has become an effective method for mining novel enzymes from the environment. Based on a deep-sea sediment metagenomics library, lipolytic-positive clones were obtained by activity-based screening methods. RESULTS:Two novel esterases, DMWf18-543 and DMWf18-558, were obtained from a deep-sea metagenomic library through activity-based screening and high-throughput sequencing methods. These esterases shared 80.7% amino acid identity with each other and were determined to be new members of bacterial lipolytic enzyme family IV. The two enzymes showed the highest activities toward p-nitrophenyl (p-NP) butyrate at pH 7.0 and 35-40 °C and were found to be resistant to some metal ions (Ba2+, Mg2+, and Sr2+) and detergents (Triton X-100, Tween 20, and Tween 80). DMWf18-543 and DMWf18-558 exhibited distinct substrate specificities and preferences. DMWf18-543 showed a catalytic range for substrates of C2-C8, whereas DMWf18-558 presented a wider range of C2-C14. Additionally, DMWf18-543 preferred p-NP butyrate, whereas DMWf18-558 preferred both p-NP butyrate and p-NP hexanoate. To investigate the mechanism underlying the phenotypic differences between the esterases, their three-dimensional structures were compared by using homology modeling. The results suggested that residue Leu199 of DMWf18-543 shortens and blocks the substrate-binding pocket. This hypothesis was confirmed by the finding that the DMWf18-558-A199L mutant showed a similar substrate specificity profile to that of DMWf18-543. CONCLUSIONS:This study characterized two novel homologous esterases obtained from a deep-sea sediment metagenomic library. The structural modeling and mutagenesis analysis provided insight into the determinants of their substrate specificity and preference. The characterization and mechanistic analyses of these two novel enzymes should provide a basis for further exploration of their potential biotechnological applications.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Huo YY,Jian SL,Cheng H,Rong Z,Cui HL,Xu XWdoi
10.1186/s12934-018-0864-4subject
Has Abstractpub_date
2018-01-30 00:00:00pages
16issue
1issn
1475-2859pii
10.1186/s12934-018-0864-4journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) containing various chain length monomers from C6 to C14 have more applications besides sustainable and environmental-friendly biomaterials owing to their superior physical and mechanical properties. We engineered a reversed fatty acid β-oxidation pathway i...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1186-x
更新日期:2019-08-13 00:00:00
abstract:BACKGROUND:A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS:Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. O...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-6-27
更新日期:2007-08-20 00:00:00
abstract:BACKGROUND:Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing attention, but there were no reports of successful implementation. Escherichia coli has been engin...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1166-1
更新日期:2019-07-05 00:00:00
abstract:BACKGROUND:Under N-limiting conditions, Saccharomyces cerevisiae strains display a substantial variability in their biomass yield from consumed nitrogen -in particular wine yeasts exhibit high growth abilities- that is correlated with their capacity to complete alcoholic fermentation, a trait of interest for fermented ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0109-0
更新日期:2014-08-19 00:00:00
abstract::Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-13-75
更新日期:2014-05-24 00:00:00
abstract:BACKGROUND:Saccharomyces cerevisiae is being exploited as a cell factory to produce fatty acids and their derivatives as biofuels. Previous studies found that both precursor supply and fatty acid metabolism deregulation are essential for enhanced fatty acid synthesis. A bacterial pyruvate dehydrogenase (PDH) complex ex...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01493-z
更新日期:2020-12-10 00:00:00
abstract:BACKGROUND:Glucose is the main carbon source of E. coli and a typical substrate in production processes. The main glucose uptake system is the glucose specific phosphotransferase system (Glc-PTS). The PTS couples glucose uptake with its phosphorylation. This is achieved by the concomitant conversion of phosphoenolpyruv...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0980-1
更新日期:2018-08-28 00:00:00
abstract::The biotechnology industry has become a key element in modern societies. Within this industry, the production of recombinant enzymes and biopharmaceutical proteins is of major importance. The global markets for such recombinant proteins are growing rapidly and, accordingly, there is a continuous need for new productio...
journal_title:Microbial cell factories
pub_type: 社论
doi:10.1186/1475-2859-12-3
更新日期:2013-01-14 00:00:00
abstract:BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-145
更新日期:2012-11-04 00:00:00
abstract:BACKGROUND:Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by lignocellulose-degrading fungi including Pycnoporus cinnabarinus. We investigated the cellulolytic system of P. cinnabarinus, focusing on the involvement of CDH in the deconstruction of lignocellulosic biomass. RESULTS:First, P. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-113
更新日期:2011-12-28 00:00:00
abstract:BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0999-3
更新日期:2018-09-21 00:00:00
abstract:BACKGROUND:Although the citric acid fermentation by Aspergillus niger is one of the most important industrial microbial processes and various aspects of the fermentation appear in a very large number of publications since the 1950s, the effect of the spore inoculum level on fungal morphology is a rather neglected area....
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-5-3
更新日期:2006-01-25 00:00:00
abstract:BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-9
更新日期:2011-02-15 00:00:00
abstract:BACKGROUND:The biocontrol strain Pseudomonas chlororaphis GP72 isolated from the green pepper rhizosphere synthesizes three antifungal phenazine compounds, 2-Hydroxyphenazine (2-OH-PHZ), 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) and phenazine-1-carboxylic acid (PCA). PCA has been a commercialized antifungal pest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0529-0
更新日期:2016-07-28 00:00:00
abstract:BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0319-0
更新日期:2015-09-15 00:00:00
abstract:BACKGROUND:Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs)...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0869-z
更新日期:2018-02-08 00:00:00
abstract:BACKGROUND:Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0936-5
更新日期:2018-06-07 00:00:00
abstract:BACKGROUND:With the development of engineering the microbial cell factories, biosensors have been used widely for regulation of cellular metabolism and high-throughput screening. However, most of the biosensors constructed in Saccharomyces cerevisiae are designed for transcriptional activation. Very few studies have de...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01405-1
更新日期:2020-07-20 00:00:00
abstract:BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0560-1
更新日期:2016-09-22 00:00:00
abstract:BACKGROUND:Cholesterol, the precursor of all steroid hormones, is the most abundant steroid in vertebrates and exhibits highly hydrophobic properties, rendering it a difficult substrate for aqueous microbial biotransformations. In the present study, we developed a Bacillus megaterium based whole-cell system that allows...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0300-y
更新日期:2015-07-29 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0099-y
更新日期:2014-08-19 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0797-3
更新日期:2017-11-02 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01346-9
更新日期:2020-04-06 00:00:00
abstract:BACKGROUND:For the cultivation of Escherichia coli in bioreactors trace element solutions are generally designed for optimal growth under aerobic conditions. They do normally not contain selenium and nickel. Molybdenum is only contained in few of them. These elements are part of the formate hydrogen lyase (FHL) complex...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-7-26
更新日期:2008-08-07 00:00:00
abstract:BACKGROUND:Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hos...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1158-1
更新日期:2019-06-13 00:00:00
abstract:BACKGROUND:The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis. RESULTS:The presence of preformed biomass precursor mol...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-13-45
更新日期:2014-03-23 00:00:00
abstract:BACKGROUND:Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gam...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0977-9
更新日期:2018-08-21 00:00:00
abstract:BACKGROUND:Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors and salts that affect the performance of the microbial production host. The performance of six industr...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-8-64
更新日期:2009-12-04 00:00:00
abstract:BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1257-z
更新日期:2019-11-25 00:00:00
abstract:BACKGROUND:Gene therapy has gained an increasing interest in its anti-tumor efficiency. However, numerous efforts are required to promote them to clinics. In this study, a novel and efficient delivery platform based on bacterial magnetosomes (BMs) were developed, and the efficiency of BMs in delivering small interferin...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0830-6
更新日期:2017-11-28 00:00:00