Abstract:
BACKGROUND:A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, is abundantly available. RESULTS:Bacillus subtilis possesses a unique inositol metabolism involving both stereoisomers. We manipulated the inositol metabolism in B. subtilis to permit the possible bioconversion from myo-inositol to scyllo-inositol. Within 48 h of cultivation, the engineered strain was able to convert almost half of 10 g/L myo-inositol to scyllo-inositol that accumulated in the culture medium. CONCLUSIONS:The engineered B. subtilis serves as a prototype of cell factory enabling a novel and inexpensive supply of scyllo-inositol.
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Yamaoka M,Osawa S,Morinaga T,Takenaka S,Yoshida Kdoi
10.1186/1475-2859-10-69subject
Has Abstractpub_date
2011-09-07 00:00:00pages
69issn
1475-2859pii
1475-2859-10-69journal_volume
10pub_type
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