Abstract:
:Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of certain membrane proteins hijack these regulation networks, leading to the formation of impressive intracellular membrane structures in both prokaryotic and eukaryotic cells. The proteins triggering an abnormal accumulation of membrane structures inside the cells (or membrane proliferation) share two major common features: (1) they promote the formation of highly curved membrane domains and (2) they lead to an enrichment in anionic, cone-shaped phospholipids (cardiolipin or phosphatidic acid) in the newly formed membranes. Taking into account the available examples of membrane proliferation upon protein overproduction, together with the latest biochemical, biophysical and structural data, we explore the relationship between protein synthesis and membrane biogenesis. We propose a mechanism for the formation of these non-physiological intracellular membranes that shares similarities with natural inner membrane structures found in α-proteobacteria, mitochondria and some viruses-infected cells, pointing towards a conserved feature through evolution. We hope that the information discussed in this review will give a better grasp of the biophysical mechanisms behind physiological and induced intracellular membrane proliferation, and inspire new applications, either for academia (high-yield membrane protein production and nanovesicle production) or industry (biofuel production and vaccine preparation).
journal_name
Microb Cell Factjournal_title
Microbial cell factoriesauthors
Royes J,Biou V,Dautin N,Tribet C,Miroux Bdoi
10.1186/s12934-020-01433-xsubject
Has Abstractpub_date
2020-09-04 00:00:00pages
176issue
1issn
1475-2859pii
10.1186/s12934-020-01433-xjournal_volume
19pub_type
杂志文章,评审abstract:BACKGROUND:β-Ionone is a fragrant terpenoid that generates a pleasant floral scent and is used in diverse applications as a cosmetic and flavoring ingredient. A growing consumer desire for natural products has increased the market demand for natural β-ionone. To date, chemical extraction from plants remains the main ap...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0984-x
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors and salts that affect the performance of the microbial production host. The performance of six industr...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-8-64
更新日期:2009-12-04 00:00:00
abstract:BACKGROUND:D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0822-6
更新日期:2017-11-21 00:00:00
abstract:BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0206-8
更新日期:2015-02-21 00:00:00
abstract:BACKGROUND:Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In f...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-11-11
更新日期:2012-01-20 00:00:00
abstract:BACKGROUND:L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS:In this study, Corynebacterium gl...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0776-8
更新日期:2017-09-22 00:00:00
abstract:BACKGROUND:Gene therapy has gained an increasing interest in its anti-tumor efficiency. However, numerous efforts are required to promote them to clinics. In this study, a novel and efficient delivery platform based on bacterial magnetosomes (BMs) were developed, and the efficiency of BMs in delivering small interferin...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0830-6
更新日期:2017-11-28 00:00:00
abstract:BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-9
更新日期:2011-02-15 00:00:00
abstract:BACKGROUND:Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0532-5
更新日期:2016-08-11 00:00:00
abstract:BACKGROUND:Protoilludene is a valuable sesquiterpene and serves as a precursor for several medicinal compounds and antimicrobial chemicals. It can be synthesized by heterologous expression of protoilludene synthase in Escherichia coli with overexpression of mevalonate (MVA) or methylerythritol-phosphate (MEP) pathway, ...
journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2016-01-19 00:00:00
abstract:BACKGROUND:L-Alanyl-L-glutamine (AQ) is a functional dipeptide with high water solubility, good thermal stability and high bioavailability. It is widely used in clinical treatment, post-operative rehabilitation, sports health care and other fields. AQ is mainly produced via chemical synthesis which is complicated, time...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01369-2
更新日期:2020-06-11 00:00:00
abstract:BACKGROUND:The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have problems such as screeni...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-9-30
更新日期:2010-05-11 00:00:00
abstract:BACKGROUND:Microalgae has been concerned as a potential source of biodiesel in the recent years. However, it is costly to harvest microalgae as it is commonly cultured in water and the cells are too small to harvest. In order to reduce the cost of cultivation and harvesting, it is important to improve the biomass produ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-014-0142-z
更新日期:2014-10-01 00:00:00
abstract::The success of recombinant protein expression seems unpredictable and even good yields of soluble proteins do not guarantee the correct folding. The search for soluble constructs can be performed by exploiting libraries and speeded up by automation, but these approaches are money and time consuming and the tags used f...
journal_title:Microbial cell factories
pub_type: 社论
doi:10.1186/1475-2859-3-12
更新日期:2004-10-07 00:00:00
abstract:BACKGROUND:The biocontrol strain Pseudomonas chlororaphis GP72 isolated from the green pepper rhizosphere synthesizes three antifungal phenazine compounds, 2-Hydroxyphenazine (2-OH-PHZ), 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) and phenazine-1-carboxylic acid (PCA). PCA has been a commercialized antifungal pest...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-016-0529-0
更新日期:2016-07-28 00:00:00
abstract:BACKGROUND:The application of chemical dispersants as a response to marine oil spills is raising concerns related to their potential toxicity also towards microbes involved in oil biodegradation. Hence, oil spills occurring under marine environments necessitate the application of biodispersants that are highly active, ...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0797-3
更新日期:2017-11-02 00:00:00
abstract:BACKGROUND:Escherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one. RESULTS:We used global transcription machinery engineering (gTME) to imp...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0368-4
更新日期:2015-11-05 00:00:00
abstract:BACKGROUND:Recombinant protein expression in bacteria often leads to the formation of intracellular insoluble protein deposits, a major bottleneck for the production of soluble and active products. However, in recent years, these bacterial protein aggregates, commonly known as inclusion bodies (IBs), have been shown to...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01375-4
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully app...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0708-7
更新日期:2017-05-31 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2012-07-27 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0381-7
更新日期:2015-11-26 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
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更新日期:2020-11-23 00:00:00
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journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01418-w
更新日期:2020-08-06 00:00:00
abstract:INTRODUCTION:Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductase...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-1036-2
更新日期:2018-12-03 00:00:00
abstract:BACKGROUND:Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed. To this end, a crucial process is translation initiation which takes place at the ribosome binding s...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-020-01404-2
更新日期:2020-07-29 00:00:00
abstract:BACKGROUND:Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host to produce proteins of medical interest, as it is "Generally Recognized As Safe". Furthermore, as L. lactis naturally secretes only one major endoge...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-015-0271-z
更新日期:2015-07-16 00:00:00
abstract:BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/1475-2859-10-36
更新日期:2011-05-18 00:00:00
abstract:BACKGROUND:Griseoviridin (GV) and viridogrisein (VG, also referred as etamycin), both biosynthesized by a distinct 105 kb biosynthetic gene cluster (BGC) in Streptomyces griseoviridis NRRL 2427, are a pair of synergistic streptogramin antibiotics and very important in treating infections of many multi-drug resistant mi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-017-0792-8
更新日期:2017-10-25 00:00:00
abstract:BACKGROUND:Bioethanol is one of the most representative eco-friendly fuels developed to replace the non-renewable fossil fuels and is the most successful commercially available bio-conversion technology till date. With the availability of inexpensive carbon sources, such as cellulosic biomass, bioethanol production has...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-019-1213-y
更新日期:2019-10-10 00:00:00
abstract:BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...
journal_title:Microbial cell factories
pub_type: 杂志文章
doi:10.1186/s12934-018-0999-3
更新日期:2018-09-21 00:00:00